Developmental alteration of hepatic UDP-glucuronosyltransferase and sulphotransferase towards androsterone and 4-nitrophenol in Wistar rats

Postnatal development of hepatic UDP-glucuronosyltransferase and sulphotransferase activities towards androsterone and 4-nitrophenol as well as cytochrome P-450 contents was studied in male and female Wistar rats. The rats with high and low UDP-glucuronosyltransferase activity towards androsterone were classified by the genotype of the parent animals. UDP-glucuronosyltransferase activity towards androsterone began rapidly to enhance after 30 days of age in the high-activity group, whereas the transferase activity remained low throughout in the low-activity group. Such a striking difference was not observed in UDP-glucuronosyltransferase activity towards 4-nitrophenol, sulphotransferase activity towards androsterone and 4-nitrophenol, and cytochrome P-450 contents. Sex-based difference in the sulphotransferase activity was marked after 30 days of age. Sulphotransferase activity towards androsterone was much higher in adult females than in adult males, whereas higher sulphation activity towards 4-nitrophenol was found in adult males. The results also indicate that the low level of the UDP-glucuronosyltransferase activity did not lead to compensatory stimulation of the sulphotransferase activity.

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Classification and genetic expression of Wistar rats with high and low hepatic microsomal UDP-glucuronosyltransferase activity towards androsterone

Biochemical Journal ◽

10.1042/bj2020171 ◽

1982 ◽

Vol 202 (1) ◽

pp. 171-174 ◽

Cited By ~ 15

Author(s):

Michio Matsui ◽

Hiroshi K. Watanabe

Keyword(s):

High Activity ◽

Wistar Rats ◽

Autosomal Dominant ◽

Transferase Activity ◽

Male And Female ◽

Genetic Expression ◽

Autosomal Dominant Fashion ◽

Female Wistar Rats ◽

Udp Glucuronosyltransferase ◽

Low Activity

Male and female Wistar rats with high and low hepatic microsomal UDP-glucuronosyltransferase activity towards androsterone were classified by partial hepatectomy. The breeding experiments between the classified high-activity and low-activity rats show that the genetic expression of the high transferase activity is inherited in an autosomal dominant fashion.

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Temperature Acclimation of Xenobiotic Metabolizing Enzymes in Cultured Hepatocytes and Whole Liver of the Gulf Toadfish, Opsanus beta

Canadian Journal of Fisheries and Aquatic Sciences ◽

10.1139/f91-146 ◽

1991 ◽

Vol 48 (7) ◽

pp. 1212-1219 ◽

Cited By ~ 21

Author(s):

Christopher J. Kennedy ◽

Kenneth A. Gill ◽

Patrick J. Walsh

Keyword(s):

Temperature Acclimation ◽

Interindividual Variation ◽

Transferase Activity ◽

Cultured Hepatocytes ◽

Metabolizing Enzymes ◽

Xenobiotic Metabolizing Enzymes ◽

Opsanus Beta ◽

Udp Glucuronosyltransferase ◽

Cytochrome P 450 ◽

Gulf Toadfish

Changes in cytochrome P-450 and microsomal and soluble protein content and the activities of several microsomal and cytosolic xenobiotic metabolizing enzymes were examined in the whole livers of gulf toadfish, Opsanus beta, following acclimation to 18 or 28 °C and in isolated hepatocytes (prepared from 18 or 28 °C acclimated gulf toadfish) which were maintained in primary culture for 29 d on L-15 media. Cells isolated from both acclimation groups were cultured at 18 and 28 °C. In whole livers, significant differences were found in the activities of sulfotransferase (which showed perfect compensation) and UDP-glucuronosyltransferase (which showed "inverse compensation") between fish acclimated to 18 and 28 °C, but several other trends were obscured by interindividual variation. However, in hepatocyte cultures, temperature adaptations were seen in cytochrome P-450 content and in the activities of the microsomal enzymes aryl hydrocarbon hydroxylase, epoxide hydrolase, and UDP-glucuronosyltransferase. Glutathione-S-transferase activity appeared to be rather temperature insensitive in both whole livers and in cultured hepatocytes. Incomplete temperature acclimation for all enzymes was observed in cultured cells and this may be due to systemic factors which were not present in cell culture media.

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Regulation of onset of development of UDP-glucuronosyltransferase activity towards o-aminophenol by glucocorticoids in late-foetal rat liver in utero

Biochemical Journal ◽

10.1042/bj1680507 ◽

1977 ◽

Vol 168 (3) ◽

pp. 507-511 ◽

Cited By ~ 21

Author(s):

G J Wishart ◽

G J Dutton

Keyword(s):

Rat Liver ◽

Alimentary Tract ◽

Normal Development ◽

In Utero ◽

Transferase Activity ◽

Foetal Rat ◽

Udp Glucuronosyltransferase ◽

Foetal Lung ◽

Precocious Development ◽

Stimulation Of

1. A precocious development of UDP-glucuronosyltransferase activity (EC 2.4.1.17) towards o-aminophenol is demonstrated in 15-17 day foetal rat liver in utero after dexamethasone administration to the mother. 2. This stimulation of liver transferase activity in utero is directly proportional to the dose of dexamethasone infected. 3. Precocious development of transferase activity in utero can also be effected with the natural glucocorticoid cortisol by multiple injections of large amounts of this hormone into the mother. 4. Transferase activity towards o-aminophenolin foetal lung, kidney and upper alimentary tract can also be precociously stimulated by dexamethasone in 17-day foetuses in utero. 5. Natural development of hepatic transferase activity between days 18 and 20 of gestation is retarded after foetal hypophysectomy by decapitation in utero. 6. Overall glucuronidation of o-aminophenol, as observed in foetal rat liver, is also precociously stimulated by dexamethasone. 7. From this and from evidence previously presented we suggest that glucocorticoids, which are known to increase in rat foetuses between days 17 and 20 of gestation, trigger the normal development in utero of hepatic transferase activity towards o-aminophenol which occurs at that time. We also suggest that these hormones are responsible for the rise in activity of the enzyme in foetal lung, kidney and upper alimentary tract which occurs during the same gestational period.

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Genetic deficiency of androsterone UDP-glucuronosyltransferase activity in Wistar rats is due to the loss of enzyme protein

Biochemical Journal ◽

10.1042/bj2340139 ◽

1986 ◽

Vol 234 (1) ◽

pp. 139-144 ◽

Cited By ~ 20

Author(s):

M Matsui ◽

F Nagai

Keyword(s):

Affinity Chromatography ◽

High Activity ◽

Wistar Rats ◽

Enzyme Protein ◽

Genetic Deficiency ◽

A Subunit ◽

Udp Glucuronosyltransferase ◽

Low Activity ◽

Hydroxy Steroid

Hepatic microsomal UDP-glucuronosyltransferases towards androsterone and testosterone were purified by chromatofocusing and UDP-hexanolamine affinity chromatography in Wistar rats which had genetic deficiency of androsterone UDP-glucuronosyltransferase activity. In rats with the high-activity phenotype, androsterone (the 3-hydroxy androgen) UDP-glucuronosyltransferase was eluted at about pH 7.4 and had a subunit Mr of 52 000, whereas testosterone (the 17-hydroxy steroid) UDP-glucuronosyltransferase was eluted at about pH 8.4 and had a subunit Mr of 50 000. The transferase that conjugates both androsterone and testosterone was eluted at about pH 8.0, had subunit Mr values of 50 000 and 52 000, and appeared to be an aggregate or hybrid of androsterone and testosterone UDP-glucuronosyltransferases. In rats with the low-activity phenotype, androsterone UDP-glucuronosyltransferase was absent, whereas testosterone UDP-glucuronosyltransferase was eluted at around pH 8.5, with a subunit Mr of 50 000.

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ANTI-ESTROGENIC PROPERTIES OF ETHANOLIC LEAF EXTRACT OF BOERHAVIA DIFFUSA ON FEMALE WISTAR RATS.

10.1055/s-0037-1608549 ◽

2017 ◽

Author(s):

C Olajide Omolola ◽

J Okpuzor ◽

I Nnadozie Jane ◽

R Adewole Adeniyi

Keyword(s):

Wistar Rats ◽

Leaf Extract ◽

Female Wistar Rats ◽

Estrogenic Properties ◽

Ethanolic Leaf Extract ◽

Boerhavia Diffusa

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NEW DATA CONCERNING THE ROLE PLAYED BY PROGESTERONE IN THE CONTROL OF FOLLICULAR GROWTH IN THE RAT

Acta Endocrinologica ◽

10.1530/acta.0.0750569 ◽

1974 ◽

Vol 75 (3) ◽

pp. 569-578 ◽

Cited By ~ 29

Author(s):

G. Buffler ◽

S. Roser

Keyword(s):

Wistar Rats ◽

Venous Blood ◽

Oestrous Cycle ◽

Female Rats ◽

Cycle Duration ◽

Follicular Growth ◽

The Third ◽

Female Wistar Rats

ABSTRACT The mechanisms involved in the prolongation of the oestrous cycle following LH administration were studied in 4-day cyclic female Wistar rats. In females injected with LH on the morning of dioestrus I there was an increase in ovarian venous blood progesterone as compared with non-injected animals. In both LH-treated females, and those injected with progesterone on the morning of dioestrus I, a slowing up in follicular growth was observed from the afternoon of dioestrus I. The size of follicles greater than 400 urn present in LH or progesterone injected animals on the third day of cycle was similar to the size reached by the same range of follicles in non-injected animals on the second day of the cycle. Hence, the increase in endogenous ovarian progesterone elicited by LH was considered as the cause of the slowing up of follicular growth and therefore of the lengthening of the oestrous cycle duration in female rats injected with LH at the beginning of 4-day cycle.

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