NEW DATA CONCERNING THE ROLE PLAYED BY PROGESTERONE IN THE CONTROL OF FOLLICULAR GROWTH IN THE RAT

1974 ◽  
Vol 75 (3) ◽  
pp. 569-578 ◽  
Author(s):  
G. Buffler ◽  
S. Roser
Keyword(s):  
Wistar Rats ◽  
Venous Blood ◽  
Oestrous Cycle ◽  
Female Rats ◽  
Cycle Duration ◽  
Follicular Growth ◽  
The Third ◽  
Female Wistar Rats

ABSTRACT The mechanisms involved in the prolongation of the oestrous cycle following LH administration were studied in 4-day cyclic female Wistar rats. In females injected with LH on the morning of dioestrus I there was an increase in ovarian venous blood progesterone as compared with non-injected animals. In both LH-treated females, and those injected with progesterone on the morning of dioestrus I, a slowing up in follicular growth was observed from the afternoon of dioestrus I. The size of follicles greater than 400 urn present in LH or progesterone injected animals on the third day of cycle was similar to the size reached by the same range of follicles in non-injected animals on the second day of the cycle. Hence, the increase in endogenous ovarian progesterone elicited by LH was considered as the cause of the slowing up of follicular growth and therefore of the lengthening of the oestrous cycle duration in female rats injected with LH at the beginning of 4-day cycle.

An analysis of the course of infection of Moniliformis dubius (Acanthocephala) in rats

Parasitology ◽  
1972 ◽  
Vol 64 (3) ◽  
pp. 517-523 ◽  
Author(s):  
D. W. T. Crompton ◽  
D. E. Walters
Keyword(s):  
Wistar Rats ◽  
Female Rats ◽  
Male Rats ◽  
Male And Female ◽  
Oral Infections ◽  
The Third ◽  
Male And Female Rats ◽  
Female Wistar Rats ◽  
Average Recovery Rate ◽  
Marked Decline

An analysis of the course of infection of mixed oral infections of 12 cystacanths of Moniliformis dubius in 174 male and 179 female Wistar rats has been undertaken.There was a marked decline in the average recovery rate of worms of both sexes from hosts of both sexes during the course of the infection.Female worms from both male and female rats showed, on average, a greater power of survival than male worms from the third period (10–13 weeks) onwards.Male rats were found to retain, on average, a greater number of worms of both sexes than female rats.We wish to thank Miss Susan Arnold and Mr David Barnard for excellent technical help.

Sex differences in spironolactone induction of rat intestinal and hepatic p-nitrophenol UDP-glucuronyltransferase

1990 ◽  
Vol 68 (10) ◽  
pp. 1385-1387 ◽  
Author(s):  
Viviana A. Catania ◽  
Marcelo G. Luquita ◽  
María C. Carrillo ◽  
Aldo D. Mottino
Keyword(s):  
Enzyme Activity ◽  
Sex Differences ◽  
Wistar Rats ◽  
Female Rats ◽  
Intestinal Tissue ◽  
Male And Female ◽  
Percent Increase ◽  
Female Wistar Rats ◽  
Related Response ◽  
Intestinal Enzyme

In the present study we analyzed the effect of spironolactone administration on hepatic and intestinal p-nitrophenol-UDP-glucuronyltransferase activity. We used microsomal preparations from male and female Wistar rats to establish whether or not this effect was sex dependent. Enzyme activity was measured in the presence of UDP-N-acetylglucosamine, a presumed physiological activator of the enzyme. Female but not male microsomes showed an increase in enzyme activity of both hepatic and intestinal tissue preparations in response to the inducer pretreatment. In addition, the inducer effect observed in female rats showed a tissue-related difference, since percent increase in the intestinal enzyme activity was greater than that in the liver (127 and 52%, respectively). These results suggest that factors regulating enzyme activity or mechanisms involved in the inducer effect of spironolactone could be different in the intestinal mucosa in comparison to the liver. A possible explanation of sex-related response to spironolactone administration was discussed.Key words: p-nitrophenol, UDP-glucuronyltransferase, spironolactone induction, sex differences.

scholarly journals Tolerance to hypothermic and antinoceptive effects of Δ9-tetrahydrocannabinol (THC) vapor inhalation in rats

2017 ◽  
Author(s):  
Jacques D. Nguyen ◽  
Yanabel Grant ◽  
Tony M. Kerr ◽  
Arnold Gutierrez ◽  
Maury Cole ◽  
...  
Keyword(s):  
Propylene Glycol ◽  
Wistar Rats ◽  
Female Rats ◽  
Male Rats ◽  
Preclinical Studies ◽  
Male And Female ◽  
Temperature Changes ◽  
Male And Female Rats ◽  
Antinociceptive Effects ◽  
Female Wistar Rats

AbstractRationaleA reduced effect of a given dose of Δ9-tetrahydrocannabinol (THC) emerges with repeated exposure to the drug. This tolerance can vary depending on THC dose, exposure chronicity and the behavioral or physiological measure of interest. A novel THC inhalation system based on e-cigarette technology has been recently shown to produce the hypothermic and antinociceptive effects of THC in rats.ObjectiveTo determine if tolerance to these effects can be produced with repeated vapor inhalation.MethodsGroups of male and female Wistar rats were exposed to 30 minutes of inhalation of the propylene glycol (PG) vehicle or THC (200 mg/mL in PG) two or three times per day for four days. Rectal temperature changes and nociception were assessed after the first exposure on the first and fourth days of repeated inhalation.ResultsFemale, but not male, rats developed tolerance to the hypothermic and antinociceptive effects of THC after four days of twice-daily THC vapor inhalation. Thrice daily inhalation for four days resulted in tolerance in both male and female rats. The plasma THC levels reached after a 30 minute inhalation session did not differ between the male and female rats.ConclusionsRepeated daily THC inhalation induces tolerance in female and male rats, providing further validation of the vapor inhalation method for preclinical studies.AbbreviationsPG, propylene glycol; THC; Δ9tetrahydrocannabinol;

scholarly journals Kefir as a Prevention of Arsenic-mediated Toxicity in Uterine Female Rats

2021 ◽  
Vol 4 (4) ◽  
Author(s):  
Sabatina Windyaningrum ◽  
◽  
Tri Yudani Mardining Raras ◽  
Bambang Rahardjo ◽  
Rose Khasana Dewi
Keyword(s):  
Protective Effect ◽  
Arsenic Trioxide ◽  
Stromal Cells ◽  
Wistar Rats ◽  
Endometrial Thickness ◽  
Normal Diet ◽  
Female Rats ◽  
Tissue Preparation ◽  
Endometrial Stromal Cells ◽  
Female Wistar Rats

Background: kefir is a fermented milk product that demonstrates numerous health benefits including antioxidant and immunomodulatory. Aim: to study the protective effect kefir on the expression of estrogen receptor alpha (ERα) in endometrial stromal cells and endometrial thickness on female rats that were exposed to arsenic. Methods: twenty-five female Wistar rats (Rattus norvegicus) were divided into five groups (CRL, As, T1, T2, T3). Control group (given a normal diet), As group (given the normal diet and exposed to arsenic trioxide 2 mg/kgBW/day). The T1; T2; T3 were exposed to arsenic trioxide 2 mg/kgBW/day and treated with different doses of kefir (1.25; 2.5; and 5 mL/kgBW/day, respectively) for 35 days. The rats of group As treated with arsenic trioxide only and group CRL served as control with normal feed in water. Cytological samples were taken after 35 days of treatment and examined every day to see the rat oestrus phase, and the proestrus phase of the oestrous cycle was chosen for termination. Uterine tissue fixed in 10% neutral buffered formalin for tissue preparation. ERα expression in endometrial stromal cells was analized using immunohistochemistry method, endometrial thickness was observed using histopathological methods. Results: significant reduction of ERα expression in endometrial stromal cells and endometrial thickness in female rats exposed to arsenic were observed in groups on treated rats (p ≤ 0.000; 0.009, respectively). Conclusion: the administration of kefir in female Wistar rats exposed to arsenic had shown significantly differences on ERα expressions and endometrial thickness. The smallest dose of kefir (1.25 mL/kgBW/day) could increase ERα expression and endometrial thickness in female Wistar rats with arsenic exposure. Therefore kefir has protective effect related to female reproductive system.

scholarly journals Estrogenic Effect of Bambusa arundinacea, Trichosanthes dioica and Punica granatum on Rats

2022 ◽  
Author(s):  
Akanksha Awasthi ◽  
Mamta F. Singh ◽  
Saurabh Sharma
Keyword(s):  
Wistar Rats ◽  
Estrogenic Activity ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Mature Female ◽  
Female Rats ◽  
Vaginal Opening ◽  
Estrogenic Effect ◽  
Female Wistar Rats ◽  
Trichosanthes Dioica

Background: Phytoestrogens have recently become a hot topic among scientists. Phytoestrogens’ estrogen-like properties have led to their widespread use in the reproductive system. The aim of this research was to see whether the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum had any estrogenic activity in female wistar rats. Methods: In female wistar rats, the estrogenic effect was studied using a uterotropic assay, vaginal cytology and vaginal opening. In ovariectomized immature and mature female wistar rats, a 400 mg/kg body weight (b.w.) dose of ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum was given. Result: When compared to ovariectomized control rats, the uterine wet weight increased significantly. The estrogen-treated rats had only cornified epithelial cells, indicating the existence of oestrogen, as well as 100% vaginal opening. At 400 mg/kg b.w., the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum demonstrated promising estrogenic activity, as evidenced by uterotropic assays, vaginal opening measurements and histopathological changes. As a result of this research, it’s possible to infer that the ethanolic extract of Bambusa arundinaceae, Trichosanthes dioica and Punica granatum play an important role in estrogenic activity in female rats.

scholarly journals Naloxone Breeding Effectiveness in Rat Suffering from Nitric Oxide-induced Polycystic Ovary Syndrome

2015 ◽  
Vol 6 (2) ◽  
pp. 67-72
Author(s):  
Manizheh Karami ◽  
Fatemeh Lakzaei ◽  
MohammadReza Jalali Nadoushan
Keyword(s):  
Nitric Oxide ◽  
Polycystic Ovary Syndrome ◽  
Wistar Rats ◽  
Polycystic Ovary ◽  
Treated Group ◽  
Female Rats ◽  
Control Group ◽  
Ovary Syndrome ◽  
Crown Rump Length ◽  
Female Wistar Rats

ABSTRACT Background and objective Polycystic ovary syndrome (PCOS) can be induced in Wistar rats by over production of nitric oxide (NO). This study evaluated the efficacy of naloxone on the breeding characteristics of rats suffering from nitric oxide induced PCOS. Materials and methods Twenty-four female Wistar rats(200–250 gm) were kept as virgin under standard conditions. They were divided into four groups (n = 6). One group of the animals received L-arginine (50 mg/kg) intraperitoneally (i.p.) for 9 days/once a day. Another group was administered naloxone hydrochloride (0.4 mg/kg, i.p.) prior to injection of L-arginine. The third group was injected solely naloxone. Control group received saline solution (1 ml/kg, i.p.). After the treatments, all female rats were coupled with the intact males. They were then separated by observation of vaginal plaques; it was considered as day 0 of pregnancy. Eventually, they were operated on days 18 to 19 of the gestation to collect the animals’ ovaries. The samples were studied for pathological evidence. The fetal number and weight along with the fetal crown-rump length (CRL) were measured. Results The ovaries obtained from the L-arginine treated group had large cysts with thickened granulosa cell layer in contrast to those of the control or naloxone treated rats (p < 0.0001). The number of fetus though showed a decrease in the L-arginine treated rats (3 ± 1), but the fetal weight or fetal CRL did not change (p > 0.05). Conclusion This study may clearly illustrate the polycystic characteristics in the L-arginine treated group. It may particularly display the breeding efficacy of naloxone in rats with PCOS. How to cite this article Karami M, Lakzaei F, Nadoushan MRJ. Naloxone Breeding Effectiveness in Rat Suffering from Nitric Oxide-induced Polycystic Ovary Syndrome. Int J Infertil Fetal Med 2015;6(2):67-72.

scholarly journals Effect of Penicillin on Reproductive Function in Female Wistar Rats

2021 ◽  
Vol 71 (2) ◽  
Author(s):  
Oyedeji K.O ◽  
Momoh R.O ◽  
Oderinde Gbenga
Keyword(s):  
Estrous Cycle ◽  
Wistar Rats ◽  
Reproductive Function ◽  
Daily Basis ◽  
Female Rats ◽  
Histopathological Study ◽  
Fertility Effect ◽  
Female Wistar Rats ◽  
Student’S T ◽  
Histopathological Studies

This study was designed to investigate the effect of penicillin on reproductive function in female Wistar rats. Fifteen female rats (120 – 160 g) were used for the estrous cycle and histopathological studies. Penicillin (17.14 mg/kg) was administered orally on daily basis for 21 and 50 days respectively for the estrous cycle and histological studies. Estrous cycle was carried out using the technique of Marcondes et al., histologies of the ovaries and uteri were also carried out. Data were analysed using descriptive statistics and student’s t-test at p=0.05. Treatment of rats for 21 days with penicillin (17.14 mg/kg) produced significant (p<0.05) increments in the estrous and metestrous phases as well as a significant (p<0.05) reduction in the proestrous phase of the estrous cycle relative to their respective controls. The histopathological study presented with a moderate endometrial congestion. It can therefore be concluded that penicillin probably has a pro-fertility effect with a moderate deleterious effect on the uteri at histological level in female Wistar rats.

Effect of imidacloprid on antioxidant status and histopathological changes in ovary and uterus of adult female wistar rats

2018 ◽  
Author(s):  
Archana Lohiya ◽  
Vinod Kumar ◽  
J.S. Punia
Keyword(s):  
Oxidative Stress ◽  
Adult Female ◽  
Wistar Rats ◽  
Control Programme ◽  
Female Rats ◽  
Histopathological Changes ◽  
Gum Acacia ◽  
Flea Control ◽  
Female Wistar Rats ◽  
Neonicotinoid Insecticide

Imidacloprid is a neonicotinoid insecticide and has been extensively used as a crop pest and in pet flea control programme. In the present study, the effects of imidacloprid on ovary and uteri tissue was analyzed in adult female Wistar rats at two dose levels (19 and 38 mg/kg/day) administered orally for 10, 20 and 30 days. Effects were compared with respective control animals administered daily with 2% gum acacia. Parameters undertaken were organ weight, levels of cytoplasmic and membrane proteins, oxidative stress parameters viz. activities of SOD, GPx and levels of GSH and MDA and histopathological changes. IMI (38 mg/kg, 30 days) reduced cytoplamic proteins in both ovaries and uteri whereas this dose reduced membrane protein in ovaries only. IMI (38 mg/kg, 20 and 30 days) decreased SOD enzyme in both ovaries and uteri and GSH-Px levels in ovaries only. The GSH-Px levels were also seen with decreased levels in uteri with IMI (38 mg/kg) for 30 days. IMI (38 mg/kg, 20 and 30 days) induces degenerative changes in ovaries of rats. Hence, it is concluded from the present studies that administration of higher doses (38 mg/kg/day) of IMI for 20 and 30 days generated oxidative stress in ovaries and uteri of female rats.

A Preliminary 13-Week Oral Toxicity Study of Ginger Oil in Male and Female Wistar Rats

2011 ◽  
Vol 30 (6) ◽  
pp. 662-670 ◽  
Author(s):  
Kottarapat Jeena ◽  
Vijayastelter B. Liju ◽  
Ramadasan Kuttan
Keyword(s):  
Wistar Rats ◽  
Hematological Parameters ◽  
Zingiber Officinale ◽  
Female Rats ◽  
Oral Toxicity ◽  
Male And Female ◽  
Male And Female Rats ◽  
Adverse Effect Level ◽  
Female Wistar Rats ◽  
Effect Level

Zingiber officinale Roscoe, ginger, is a major spice extensively used in traditional medicine. The toxicity profile of ginger oil was studied by subchronic oral administration for 13 weeks at doses of 100, 250, and 500 mg/kg per day to 6 groups of Wistar rats (5/sex per dose). Separate groups of rats (5/sex per group) received either paraffin oil (vehicle) or were untreated and served as comparative control groups. There was no mortality and no decrease in body weight or food consumption as well as selective organ weights during the study period. Administration of ginger oil to rats did not produce any treatment-related changes in hematological parameters, hepatic, renal functions, serum electrolytes, or in histopathology of selected organs. The major component of ginger oil was found to be zingiberene (31.08%), and initial studies indicated the presence of zingiberene in the serum after oral dosing. These results confirmed that ginger oil is not toxic to male and female rats following subchronic oral administrations of up to 500 mg/kg per day (no observed adverse effect level [NOAEL]).

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