The role of histone acetylation in phosphate regulated expression from the PHOS gene of Saccharomyces cerevisiae

Abstract REC104 is a gene required for the initiation of meiotic recombination in Saccharomyces cerevisiae. To better understand the role of REC104 in meiosis, we used an in vitro mutagenesis technique to create a set of temperature-conditional mutations in REC104 and used one ts allele (rec104-8) in a screen for highcopy suppressors. An increased dosage of the early exchange gene REC102 was found to suppress the conditional recombinational reduction in rec104-8 as well as in several other conditional rec104 alleles. However, no suppression was observed for a null allele of REC104, indicating that the suppression by REC102 is not “bypass” suppression. Overexpression of the early meiotic genes REC114, RAD50, HOP1, and RED1 fails to suppress any of the rec104 conditional alleles, indicating that the suppression might be specific to REC102.

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Role of Oxidative Phosphorylation in Histatin 5-Induced Cell Death in Saccharomyces cerevisiae

Biotechnology Letters ◽

10.1007/s10529-004-4608-7 ◽

2004 ◽

Vol 26 (23) ◽

pp. 1781-1785 ◽

Cited By ~ 6

Author(s):

Kris De Smet ◽

Rieka Reekmans ◽

Roland Contreras

Keyword(s):

Saccharomyces Cerevisiae ◽

Cell Death ◽

Oxidative Phosphorylation ◽

Histatin 5

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The ketogenic diet increases Neuregulin 1 expression via elevating histone acetylation and its anti-seizure effect requires ErbB4 kinase activity

Cell & Bioscience ◽

10.1186/s13578-021-00611-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Jin Wang ◽

Jie Huang ◽

Shan Yao ◽

Jia-Hui Wu ◽

Hui-Bin Li ◽

...

Keyword(s):

Histone Acetylation ◽

Ketogenic Diet ◽

Chromatin Immunoprecipitation ◽

Kinase Activity ◽

Control Diet ◽

Synaptic Activity ◽

Therapeutic Interventions ◽

Type I ◽

Neuregulin 1

Abstract Background The ketogenic diet (KD)has been considered an effective treatment for epilepsy, whereas its underlying mechanisms remain obscure. We have previously reported that the KD feeding increased Neuregulin 1 (NRG1) expression in the hippocampus; disruption of NRG1 signaling by genetically deleting its receptor-ErbB4 abolished KD’s effects on inhibitory synaptic activity and seizures. However, it is still unclear about the mechanisms underlying the effect of KD on NRG1 expression and whether the effects of KD require ErbB4 kinase activity. Methods The effects of the KD on NRG1 expression were assessed via western blotting and real-time PCR. Acetylation level at the Nrg1 promoter locus was examined using the chromatin immunoprecipitation technique. Kainic acid (KA)-induced acute seizure model was utilized to examine the effects of KD and histone deacetylase inhibitor-TSA on seizures. Synaptic activities in the hippocampus were recorded with the technique of electrophysiology. The obligatory role of ErbB4 kinase activity in KD’s effects on seizures and inhibitory synaptic activity was evaluated by using ErbB kinase antagonist and transgenic mouse-T796G. Results We report that KD specifically increases Type I NRG1 expression in the hippocampus. Using the chromatin immunoprecipitation technique, we observe increased acetylated-histone occupancy at the Nrg1 promoter locus of KD-fed mice. Treatment of TSA dramatically elevates NRG1 expression and diminishes the difference between the effects of the control diet (CD) and KD. These data indicate that KD increases NRG1 expression via up-regulating histone acetylation. Moreover, both pharmacological and genetic inhibitions of ErbB4 kinase activity significantly block the KD’s effects on inhibitory synaptic activity and seizure, suggesting an essential role of ErbB4 kinase activity. Conclusion These results strengthen our understanding of the role of NRG1/ErbB4 signaling in KD and shed light on novel therapeutic interventions for epilepsy.

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Role of the CDC8 gene in the repair of single strand breaks in DNA of the yeast Saccharomyces cerevisiae

Current Genetics ◽

10.1007/bf00318376 ◽

1990 ◽

Vol 18 (3) ◽

pp. 175-179 ◽

Cited By ~ 2

Author(s):

H. Baranowska ◽

D. Zaborowska ◽

W. J. Jachymczyk ◽

J. Żuk

Keyword(s):

Saccharomyces Cerevisiae ◽

Single Strand ◽

Yeast Saccharomyces Cerevisiae ◽

Strand Breaks ◽

Single Strand Breaks

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Role of cations in the flocculation of Saccharomyces cerevisiae and discrimination of the corresponding proteins

Canadian Journal of Microbiology ◽

10.1139/m91-064 ◽

1991 ◽

Vol 37 (5) ◽

pp. 397-403 ◽

Cited By ~ 17

Author(s):

Hiroshi Kuriyama ◽

Itaru Umeda ◽

Harumi Kobayashi

Keyword(s):

Saccharomyces Cerevisiae ◽

Cell Surface ◽

Inhibitory Effect ◽

Surface Proteins ◽

Promoting Effect ◽

Yeast Strains ◽

Yeast Flocculation ◽

Different Types ◽

Anionic Groups

Asexual yeast flocculation was studied using strong flocculents of Saccharomyces cerevisiae. The inhibitory effect of cations on flocculation is considered to be caused by competition between those cations and Ca2+ at the binding site of the Ca2+-requiring protein that is involved in flocculation. Inhibition of flocculation by various cations occurred in the following order: La3+, Sr2+, Ba2+, Mn2+, Al3+, and Na+. Cations such as Mg2+, Co2+, and K+ promoted flocculation. This promoting effect may be based on the reduction of electrostatic repulsive force between cells caused by binding of these cations anionic groups present on the cell surface. In flocculation induced by these cations, trace amounts of Ca2+ excreted on the cell surface may activate the corresponding protein. The ratio of Sr2+/Ca2+ below which cells flocculated varied among strains: for strains having the FLO5 gene, it was 400 to 500; for strains having the FLO1 gene, about 150; and for two alcohol yeast strains, 40 to 50. This suggests that there are several different types of cell surface proteins involved in flocculation in different yeast strains. Key words: yeast, flocculation, protein, cation, calcium.

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