A PCR-RFLP typing method for adhesion molecule gene polymorphisms and allele frequencies in a normal UK population

2002 ◽  
Vol 29 (2) ◽  
pp. 109-111 ◽  
Author(s):  
R. A. Gbadegesin ◽  
C. J. Watson ◽  
S. A. Cotton ◽  
P. E. C. Brenchley ◽  
N. J. A. Webb
Keyword(s):  
Adhesion Molecule ◽  
Gene Polymorphisms ◽  
Allele Frequencies ◽  
Typing Method ◽  
Pcr Rflp ◽  
Adhesion Molecule Gene ◽  
Rflp Typing

Rapid Molecular Typing of Methicillin-ResistantStaphylococcus aureusby PCR-RFLP

2001 ◽  
Vol 22 (5) ◽  
pp. 294-298 ◽  
Author(s):  
Thomas A. Wichelhaus ◽  
Klaus-Peter Hunfeld ◽  
Boris Böddinghaus ◽  
Peter Kraiczy ◽  
Volker Schàfer ◽  
...  
Keyword(s):  
Fragment Length Polymorphism ◽  
Restriction Analysis ◽  
Protein A ◽  
Hypervariable Region ◽  
Epidemiological Surveillance ◽  
Typing Method ◽  
Rapid Pcr ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Rflp Typing

AbstractObjective:To establish a new, rapid, and reliable genotypic fingerprinting technique for methicillin-resistantStaphylococcus aureus(MRSA) typing in routine epidemiological surveillance.Design:The method is based on polymerase chain reaction (PCR) restriction fragment-length polymorphism (RFLP) followingHaeII digestion of simultaneously amplified parts of the protein A gene, the coagulase gene, and the hypervariable region adjacent tomecA. A total of 46 MRSA initial isolates were analyzed, including 14 isolates from five countries; the six German epidemic strains; 16 isolates from the Frankfurt metropolitan area, which were known to be heterogeneous by pulsed-field gel electrophoresis (PFGE); and 10 isolates obtained during three epidemics, all of which displayed an identical genotype.Results:Restriction analysis by PCR-RFLP permitted discrimination of 10 of 14 international isolates, all six German epidemic strains, and 15 of 16 national isolates. It also confirmed the homogeneous character of the 10 outbreak isolates.Conclusions:This new and rapid PCR-RFLP typing method is an attractive tool in routine epidemiological surveillance. Its impressive characteristics are ease of performance and interpretation, while at the same time guaranteeing good discriminatory power, reproducibility, and typeability.

scholarly journals Analysis of methicillin-resistant and methicillin-susceptibleStaphylococcus aureusby a molecular typing method based on coagulase gene polymorphisms

1995 ◽  
Vol 115 (3) ◽  
pp. 419-426 ◽  
Author(s):  
N. Kobayashi ◽  
K. Taniguchi ◽  
K. Kojima ◽  
S. Urasawa ◽  
N. Uehara ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Gene Polymorphisms ◽  
Fragment Length Polymorphism ◽  
Restriction Enzyme Digestion ◽  
Rflp Pattern ◽  
Typing Method ◽  
Methicillin Resistant ◽  
Serological Typing ◽  
Molecular Typing Method ◽  
Rflp Typing

SummaryA molecular typing method forStaphylococcus aureusbased on coagulase gene polymorphisms (coagulase gene typing) was evaluated by examining a total of 240 isolates which comprised 210 methicillin-resistantS. aureus(MRSA) and 30 methicillin-susceptibleS. aureus(MSSA) collected from a single hospital. ByAlulrestriction enzyme digestion of the PCR-amplified 3′-end region of the coagulase gene including 81-bp repeated units, the MRSA and MSSA isolates examined were divided into 6 and 12 restriction fragment length polymorphism (RFLP) patterns, respectively, whereas five patterns were commonly detected in MRSA and MSSA. MRSA isolates that showed a particular RFLP pattern were considered to be predominant in the hospital. Coagulase typing with type-specific antisera was also performed for allS. aureusisolates for comparison. Coagulase types II and VII were most frequently detected and included isolates with four and five differentAluIRFLP patterns, respectively, whereas each of the other coagulase types corresponded to a single RFLP pattern. These results indicated that RFLP typing was more discriminatory than serological typing, for typingS. aureusand demonstrated its utility in epidemiologic investigation ofS. aureusinfection in hospitals.

Association of cell adhesion molecule gene polymorphisms with recurrent aphthous stomatitis

2013 ◽  
Vol 42 (10) ◽  
pp. 741-746 ◽  
Author(s):  
Asem Alkhateeb ◽  
Jumana Karasneh ◽  
Hebah Abbadi ◽  
Ahmad Hassan ◽  
Martin Thornhill
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Gene Polymorphisms ◽  
Cell Adhesion Molecule ◽  
Recurrent Aphthous Stomatitis ◽  
Aphthous Stomatitis ◽  
Adhesion Molecule Gene

A PCR-RFLP typing method for the caprine Mhc class II DRB gene

1996 ◽  
Vol 55 (1-3) ◽  
pp. 255-260 ◽  
Author(s):  
M. Amills ◽  
O. Francino ◽  
A. Sànchez
Keyword(s):  
Mhc Class Ii ◽  
Class Ii ◽  
Typing Method ◽  
Pcr Rflp ◽  
Drb Gene ◽  
Rflp Typing

scholarly journals Investigation of an outbreak of ciprofloxacin-resistant Neisseria gonorrhoeae using a simplified opa-typing method

2001 ◽  
Vol 126 (2) ◽  
pp. 219-224 ◽  
Author(s):  
H. M. PALMER ◽  
J. P. LEEMING ◽  
A. TURNER
Keyword(s):  
United Kingdom ◽  
Neisseria Gonorrhoeae ◽  
Significant Problem ◽  
Resistant Clone ◽  
Typing Method ◽  
Antibiotic Resistant ◽  
The North ◽  
The United Kingdom ◽  
South Wales ◽  
Pcr Rflp

Ciprofloxacin-resistant gonococci have been isolated from patients in the United Kingdom since 1993. Until recently, evidence has suggested that the majority of infections are not endemic but have been acquired abroad. In October 1999, increasing numbers of ciprofloxacin resistant isolates of the non-requiring auxotype were reported in Oldham and Rochdale (Northwest England). These and similar isolates from elsewhere in England and Wales were genetically characterized using a simplified opa-typing method (a non-radioactive PCR–RFLP method targeting the opa family of genes). Of 73 isolates studied, 24 had unique opa-types (10 from infections acquired abroad), whilst the remaining 49 were indistinguishable (none were known to be acquired abroad). This cluster included 31 isolates from Oldham and Rochdale, 16 from elsewhere in the north of England, and 2 from Southern England and South Wales with known epidemiological links to cases from Manchester and Rochdale respectively. This study illustrates the potential for spread of an antibiotic resistant clone of N. gonorrhoeae both locally and nationally and demonstrates that endemic acquisition of ciprofloxacin-resistant gonococci is now a significant problem in the United Kingdom.

scholarly journals The Association of XRCC1 Gene Polymorphisms and Chronic Hepatitis C Induced Insulin Resistance in Egyptian Patients

Cells ◽  
2018 ◽  
Vol 7 (11) ◽  
pp. 185
Author(s):  
Salwa Abo El-khair ◽  
Mona Arafa ◽  
Tarek Besheer ◽  
Ahmed El-Eraky ◽  
Ayman Elsamanoudy
Keyword(s):  
Insulin Resistance ◽  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Chronic Hepatitis C ◽  
Gene Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Xrcc1 Gene ◽  
Egyptian Patients ◽  
Pcr Rflp ◽  
Chronic Hcv

Chronic hepatitis C is implicated in insulin resistance (IR) susceptibility. An X-ray repair cross-complementing group 1 gene (XRCC1) is proposed to be a candidate gene for a study of IR susceptibility. So, this study aims to investigate the possible association of the XRCC1 gene polymorphisms with the risk of IR related to chronic hepatitis C virus (HCV) infection in Egyptian patients. In a case-control study, a total of 210 subjects, including 140 chronic HCV patients (87 patients with IR and 53 without IR) and 70 healthy controls, were included. Two genetic polymorphisms (c.1254C > T and c.1517G > C) of the XRCC1 gene were genotyped via the PCR-restriction fragment length polymorphism (PCR-RFLP) technique. The result of the current study revealed that these two single nucleotide polymorphisms (SNPs) have statistically significant influences on susceptibility to IR in chronic HCV infected Egyptian patients. It could be concluded that c.1254C > T, the TT genotype, CT/CC carriers as well as c.1517G > C, the CC genotype and GC/GG carriers might be associated with increased IR susceptibility. Moreover, T-allele of c.1254C > T and the C-allele of c.1517G > C genetic variants might influence the susceptibility.

scholarly journals Evaluation of Frequency of PGC1-α and CKMM Genes Polymorphisms Among Iranian Elite Hockey Athletes

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Maryam Valipour ◽  
Parvaneh Azarali ◽  
Rostam Alizadeh
Keyword(s):  
Physical Activity ◽  
Environmental Factors ◽  
Sample Size ◽  
Dna Extraction ◽  
Gene Polymorphisms ◽  
Small Sample Size ◽  
Small Sample ◽  
Complex Phenotype ◽  
Genotype Frequencies ◽  
Pcr Rflp

Background: Physical activity is a complex phenotype influenced by millions of genes and environmental factors. It is well known that physical performance and sports ability are linked to genes variations. Objectives: The aim of this study was to evaluate the frequency of PGC1-α and CKMM gene polymorphisms in elite hockey athletes. Methods: Fifty Iranian elite national hockey athletes and 100 non-athletes participated in this study. To determine the genotypes of PGC1-α and CKMM gene polymorphisms, 2 ml of saliva was sampled and used for DNA extraction. To determine the genotypes, the PCR-RFLP method was employed. After examining the variants, the allele and genotype frequencies of subjects were measured. Results: The results showed no significant differences for the PPARGC1A gene in the percentage of AA, GG, and AG genotypes. Similarly, the percentage of these genotypes of the CKMM gene did not differ significantly between athletes and non-athletes. Conclusions: The results suggest that the gene polymorphisms of PGC1-α and CKMM are the same between the Iranian elite hockey athletes and non-athletes, which may be due to the overlapping effect of other genes and/or the small sample size of the study.

scholarly journals Association between osteoporosis and polymorphisms of the bone estrogen receptor 1, calcitonin receptor genes in Mongolian postmenopausal women

2014 ◽  
Author(s):  
Unentsatsral Lkhagvasuren ◽  
Sarantuya Jav ◽  
Ochbadrakh Batjargal ◽  
Myagmarsuren Batsukh
Keyword(s):  
Estrogen Receptor ◽  
Postmenopausal Women ◽  
Genomic Dna ◽  
Venous Blood ◽  
Allele Frequencies ◽  
Calcitonin Receptor ◽  
Significant Difference ◽  
Pcr Rflp ◽  
Estrogen Receptor 1 ◽  
Genotype And Allele Frequencies

In this study, we have investigated the association between osteoporosis and estrogen receptor 1 (ER1) 397 T>C, and calcitonin receptor (CALCR) 1340 T>C polymorphisms. Genomic DNA was obtained from 104 persons (52 osteoporotic and 52 healthy controls). Genomic DNA was extracted from EDTA-preserved peripheral venous blood of patients and controls and analyzed by PCR-RFLP. As a result, there was no statistically significant difference in the genotype and allele frequencies of patients and controls for ER1 397 T>C and CALCR 1340 T>C polymorphisms. ER1 CC and TC single nucleotides genotypes compared with TT genotypes was found more significantly women with osteoporosis [p=0.016; p=0.0046, OR=2.66; 0.44, 95% CI 1.185-5.988; 0.199-0.991)]. There was no statistically significant difference in the genotype and allele frequencies of patient and controls for ER1 combined nucleotides [p=0,148, OR=1.051, 95% CI (0.993–1.112)]. Our study showed that CALCR genes single and combined nucleotides genotypes were not significant women with osteoporotic and healthy postmenopausal women.

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