The allele frequencies of growth hormone gene on the parental and progeny of Ongole-crossbreed cattle population in the North Sulawesi of Indonesia using PCR-RFLP

Background: Growth Hormone (GH) gene codes for the growth hormone, an anabolic hormone synthesized and secreted by the somatotroph cells of pituitary. Growth hormone influences many processes such as growth, lactation, reproduction and metabolism. Genetic variation in the gene are utilized as markers for selecting animals that are superior in terms growth, production and reproduction traits. Information available on the polymorphism of growth hormone gene of Ganjam and Baigani goats of Odisha is scanty. The current investigation was done to explore the genetic variation of this gene and its association with morphometric traits. Methods: Genetic polymorphism in exon 2 and 3 of Growth hormone gene in Ganjam goat and Baigani goat was explored with sample size of 100 goats for each. The goats belonged to three locations: Khallikote, Rambha, Chattrapur of Ganjam district. The goats were recorded for their body weights, morphometric traits and morphological traits. Genomic DNA was isolated, the target segment comprising exons 2 and 3 was amplified and PCR-RFLP was carried out using Hae III restriction enzyme. Genotypes were scored. Result: PCR of the locus resulted in 422 bp PCR product. PCR-RFLP using Hae III restriction enzyme yielded only two variants in both the populations. The variant A had only one restriction recognition site on the target gene segment yielding two bands with size of 366bp and 56bp whereas the variant B did not have any restriction site with single band of 422bp. Three genotypes AA, AB and BB were found in both the population. In both the population, the gene and genotype frequency were significantly deviated from the Hardy Weinberg Equilibrium frequency. There was a significant difference in the genotype frequencies of growth hormone gene between Ganjam and Baigani goats. The AB genotype had higher mean value for all morphometric traits than AA and BB genotype though the differences were not found to be significant.

Download Full-text

Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia

Archives Animal Breeding ◽

10.5194/aab-61-481-2018 ◽

2018 ◽

Vol 61 (4) ◽

pp. 481-489 ◽

Cited By ~ 2

Author(s):

Sihem Amiri ◽

Bayrem Jemmali ◽

Mohamed Amine Ferchichi ◽

Hajer Jeljeli ◽

Rekik Boulbaba ◽

...

Keyword(s):

Growth Hormone ◽

Dairy Cattle ◽

Reproductive Traits ◽

Mixed Linear Model ◽

Favorable Effect ◽

Growth Hormone Gene ◽

Nucleotide Polymorphisms ◽

Pcr Rflp ◽

The Difference ◽

Bovine Species

Abstract. Research to assess the effect of single genes on reproductive traits in bovine species is imperative to elucidate genes' functions and acquire a better perspective of quantitative traits. The present study was undertaken to characterize genetic diversity in the bovine growth hormone (GH) gene in a population of 410 Holstein dairy cows in Tunisia. The analyses were based on single nucleotide polymorphisms, and GH-AluI and GH-MspI detections and genotyping were carried out using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Data were analyzed using a mixed linear model with the MIXED procedure to reveal the possible effect of GH genotypes on reproductive traits. The frequency data of AluI(L//V) and MspI(+//−) alleles were 87.04//12.96 and 70.06//29.94, respectively. The distribution of the frequency of GH genotypes for LL/LV/VV and (−/−)//(+/−)//(+/+) were 77.75//18.59//3.66 and 15.37//29.13//55.50, respectively. The results of the statistical analyses proved that GH-AluI showed a substantial favorable effect on exanimate traits except for the age at first calving; however, only a suggestive effect of GH-MspI on the calving interval (CI) and the days open (DI) was found. The homozygous LL genotype seemed to be advantageous with respect to the CI and the DI compared with LV and VV genotypes. Heterozygous MspI(+/−) cows tended to have a longer CI and DI than MspI(+/+) and MspI(−/−) cows, but the difference was not statistically significant. A significant effect of different GH-AluI–MspI combined genotypes was found on the number of inseminations per conception, the CI and the DI, and the LL/– combined genotype seemed to be associated with better reproductive performance. Based on these results, the LL genotype of the GH locus can be considered to be a favorable genotype for reproductive traits in Holstein dairy cattle, although these findings need to be confirmed by further research before polymorphisms can be used in a marker-assisted selection program.

Download Full-text

Associations between the AluI polymorphism of growth hormone gene and production and reproduction traits in a Hungarian Holstein-Friesian bull dam population

Archives Animal Breeding ◽

10.5194/aab-49-236-2006 ◽

2006 ◽

Vol 49 (3) ◽

pp. 236-249 ◽

Cited By ~ 2

Author(s):

K. Kovács ◽

J. Völgyi-Csík ◽

A. Zsolnai ◽

I. Györkös ◽

L. Fésüs

Keyword(s):

Growth Hormone ◽

Milk Fat ◽

Milk Composition ◽

Statistical Analyses ◽

Bovine Growth Hormone ◽

Growth Hormone Gene ◽

Genotype Distribution ◽

Holstein Friesian ◽

Pcr Rflp ◽

Reproduction Traits

Abstract. The aim of this paper was to study the polymorphisms of bovine growth hormone gene. The authors genotyped 363 Hungarian Holstein-Friesian bull dams from 6 farms all over the country. Two variants (L and V) of the bovine growth hormone gene digested with AluI enzyme were identified in the experiment. Genotyping was carried out by PCR-RFLP method. The frequency data of L and V allele was 0.93 and 0.07 respectively. Distribution of the three genotypes were 87.05% (LL), 12.40% (LV) and 0.55% (VV). The studied population was in H-W equilibrium considering the genotype distribution. SPSS 11.0 for Windows was used to reveal the possible correlations between GH genotypes and production and reproduction traits and further statistical analyses. On the basis of statistical analyses it can be found that VV genotype cows had the longest milking period and LL had the shortest dry period. Both differences were significant. Cows with LV genotype had significantly higher test milking data than LL cows. Furthermore, LV genotype seemed to be advantageous for 305 days lactation milk yield. While milk composition traits, as 305 days milk fat and protein percent showed the opposite tendency, since LL genotyped dams produced significantly higher values in these traits.

Download Full-text

Philogenic Relationship of Wild Pigs and Local Pig from North Sulawesi Based on the Growth Hormone Gene (GH Gene)

Materials Science Forum ◽

10.4028/www.scientific.net/msf.967.71 ◽

2019 ◽

Vol 967 ◽

pp. 71-82

Author(s):

Alexius Mege Revolson ◽

Yermia Semuel Mokosuli ◽

Jacqueline Jochebed Rayer Debby ◽

Ellen Hetie Adil ◽

Christny Rompas ◽

...

Keyword(s):

Growth Hormone ◽

Growth Hormone Gene ◽

Wild Pigs ◽

North Sulawesi ◽

Extraction And Purification ◽

Gh Gene ◽

Pcr Method ◽

Local Pigs ◽

Key Words Growth Hormone ◽

Relationship Of

Growth hormone regulates reproduction and growth in mammals. A study was conducted to obtain the characteristics of the GH gene, in local pigs in North Sulawesi. Pig samples were obtained from traditional farmers, from four districts in North Sulawesi. DNA extraction and purification, using pig pituitary tissue. Amplification of GH gene, performed by PCR method. Visualization of CO1 gene amplikon, performed by electrophoresis technique. Sequencing, conducted through the First BASE Singapore sequencing service. The results show that there is a variation of local pigs CO1 gene in North Sulawesi. Variations are also found in the amino acid sequence encoded by the GH gene. Knowledge of the characteristics of local pig gh gene, the basics of selection of local pigs superior to North Sulawesi. Key words : growth hormone gen, local pigs, Sulawesi Utara Abstrak Gen growth hormone meregulasi reproduksi dan pertumbuhan pada mamalia. Telah dilaksanakan penelitian yang bertujuan untuk mendapatkan karakteristik gen GH pada babi lokal di Sulawesi Utara. Sampel babi diperoleh dari peternak tradisonal dari empat kabupaten di Sulawesi Utara. Ekstraksi dan purifikasi DNA menggunakan jaringan hipofisis babi. Amplifikasi gen GH menggunakan metode PCR. Visualisasi amplikon gen CO1 dilakukan dengan teknik elektroforesis. Sekuensing dilakukan melalui jasa layanan sekuensing First BASE Singapura. Hasil penelitian menunjukkan bahwa terdapat variasi gen CO1 babi lokal di Sulawesi Utara. Variasi juga ditemukan pada urutan asam amino yang dikode oleh gen GH. Diketahuinya karakteristik gen GH babi lokal, menjadi dasar seleksi babi lokal unggul Sulawesi Utara.

Download Full-text

GROWTH HORMONE GENE GENOTYPING BY Msp I RESTRICTION ENZYME AND PCR-RFLP METHODS IN ACEH CATTLE BREED AT INDRAPURI DISTRICT OF ACEH PROVINCE

Journal of the Indonesian Tropical Animal Agriculture ◽

10.14710/jitaa.38.4.207-211 ◽

2013 ◽

Vol 38 (4) ◽

Author(s):

W.P.B. Putra ◽

T. Hartatik ◽

S. Sumadi

Keyword(s):

Growth Hormone ◽

Restriction Enzyme ◽

Cattle Breed ◽

Growth Hormone Gene ◽

Pcr Rflp ◽

Aceh Province

Download Full-text

Changthangi Goats: A rich source of pashmina production in Ladakh

Animal Genetic Resources Information ◽

10.1017/s1014233900001826 ◽

2004 ◽

Vol 35 ◽

pp. 75-85 ◽

Cited By ~ 1

Author(s):

T.K. Bhattacharya ◽

S.S. Misra ◽

F.D. Sheikh ◽

P. Kumar ◽

A. Sharma

Keyword(s):

Growth Hormone ◽

Large Scale ◽

Fibre Diameter ◽

The Body ◽

Whole Body ◽

Growth Hormone Gene ◽

Body Colour ◽

Male And Female ◽

Pcr Rflp ◽

Preliminary Study

SummaryPashmina, internationally known as “cashmere”, a fine luxury fibre, is being produced from Changthangi goats bred in the Ladakh region of India. The Leh district of Greater Ladakh produces around 30 000 kg of pashmina fibre which is harvested from about 0.15 million Changthangi goats reared by the Changpa nomads in Changthang region of Greater Ladakh. Changthangi goats are sometimes also called Changra goats. Pashmina producing goats are of great importance for revitalising the economy of the poverty stricken region of Changthang and the Leh district of Ladakh. The information on Changthangi goats was collected from both small and large-scale farmers in Ladakh, and 337 animals were included in the study.The body colour of Changthangi goats varies from white to light brown and nearly whole body is covered with pashmina and long hairs. The average birth weights of male and female kids were estimated as 2.11 ± 0.3 and 2.06 ± 0.2 kg, respectively while weight at 300 days was found to be 20.0 ± 2.1 and 18.7 ± 1.9 kg in male and females respectively.The pashmina yields of bucks, does, male hoggets and female hoggets were estimated as 402 ± 19 g, 248 ± 14 g, 255 ± 12 and 280 ± 16 g, respectively. The length of pashmina fibre was found to be 4.25 ± 1.2 cm in males and 4.02 ± 1.5 cm in females while fibre diameter in male and female goats was estimated as 12.9 ± 2.6 μ and 13.0 ± 3.0 μ, respectively.The twining rate was found to be very low, nearly 0.3%. A preliminary study at DNA level with PCR-RFLP indicated monomorphism at the growth hormone gene. The occurrence of disease was quite low although some genetic deformities in this breed were not uncommon.

Download Full-text

IDENTIFIKASI POLIMORFISME GEN GH (GROWTH HORMONE) SAPI BALI DENGAN METODE PCR-RFLP

Journal of Biological Researches ◽

10.23869/bphjbr.12.1.20062 ◽

2006 ◽

Vol 12 (1) ◽

pp. 7-11 ◽

Cited By ~ 1

Author(s):

Sri Rahayu ◽

Sutiman Bambang Sumitro ◽

T Susilawati ◽

Soemarno Soemarno

Keyword(s):

Growth Hormone ◽

Restriction Enzyme ◽

Growth Hormone Gene ◽

Salting Out ◽

Pcr Product ◽

Pcr Rflp ◽

Reverse Primer ◽

Total Dna ◽

Polymerase Chain ◽

Forward Primer

This study was conducted to identify polymorphism of growth hormone gene of Bali cattle. A PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) procedure was developed for determining polymorphism of growth hormone gene. The DNA was isolated from blood samples by salting out method. Total DNA were amplified with forward primer, 5’-TAGGGGAGGGTGGAAAATGGA-3’ and reverse primer, 5’-GACACCTACTCAGACAATGCG-3’. The PCR product was digested by HaeIII restriction enzyme. Result of the amplification was a specific single band with fragment 450 bp. Restriction with HaeIII restriction enzyme resulted four kinds of haplotype. Haplotype I was not cut by HaeIII restriction enzyme. Haplotype II were cut into two, 225 bp and 150 bp,. Haplotype III were cut into three size, 400 bp, 225 bp and 150 bp. Haplotype IV were cut into five fragments 450 bp, 400 bp, 275 bp, 225 bp and 150 bp.

Download Full-text

Genetic Polymorphism of Growth Hormone Gene Exon-4 in Surti and Mehsani Goats by PCR- RFLP

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.v14i1.12993 ◽

2018 ◽

Vol 14 (1) ◽

Author(s):

Jyotishree Bayan ◽

Vishnu Kharadi ◽

Umed Ramani ◽

Mamta Janmeda ◽

Kuldeep Tyagi ◽

...

Keyword(s):

Growth Hormone ◽

Fragment Length Polymorphism ◽

Allelic Frequency ◽

Growth Hormone Gene ◽

Chain Reaction ◽

Pcr Rflp ◽

Gh Gene ◽

Polymerase Chain ◽

Exon 4 ◽

Gene Exon

The present investigation was planned to study growth hormone (GH) gene exon-4 polymorphism using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) in Surti and Mehsani goats. GH gene exon-4 region was found to be monomorphic on restriction digestion with HaeIII, which revealed only one genotype CC in both Surti and Mehsani goat breeds. The allelic frequency of C was 1.00 in both the breeds of goats with absence of D allele.

Download Full-text