A new model of orthotopic penetrating corneal transplantation in the sheep: Graft survival, phenotypes of graft-infiltrating cells and local cytokine production

1999 ◽  
Vol 27 (2) ◽  
pp. 127-135 ◽  
Author(s):  
Ka Williams ◽  
Sd Standfield ◽  
MedLabCert ◽  
Rad Mills ◽  
T Takano ◽  
...  
Keyword(s):  
Graft Survival ◽  
Cytokine Production ◽  
Corneal Transplantation ◽  
New Model ◽  
Infiltrating Cells ◽  
Local Cytokine

Ripasudil zur antiangiogenen Therapie bei Keratoplastik

2021 ◽  
pp. 1-2
Author(s):  
Björn Bachmann
Keyword(s):  
Graft Survival ◽  
Immune Cell ◽  
Kinase Inhibitor ◽  
Healing Process ◽  
Corneal Transplantation ◽  
Inflammatory Factors ◽  
Wound Healing Process ◽  
Mrna Levels ◽  
Allograft Survival ◽  
Corneal Allograft

Corneal allograft survival is mediated by the variety of immunological reactions and wound healing process. Our aim was to explore the effects of topical administration of ripasudil, a selective Rho-associated coiled-coil protein kinase inhibitor, on corneal allograft survival. Ripasudil was administered to mice thrice a day after allogeneic corneal transplantation. Corneal graft survival, opacity, neovascularization, re-epithelization, immune cell infiltration, and mRNA levels of angiogenic and pro-inflammatory factors in the grafted cornea and draining lymph nodes (dLNs) were evaluated with slit-lamp microscopy, immunohistochemistry, flow cytometry, and polymerase chain reaction. Graft survival was significantly prolonged with lower graft opacity and neovascularization scores in 0.4% and 2.0% ripasudil-treated groups, and mRNA levels of angiogenic and pro-inflammatory factors in ripasudil-treated grafted corneas were reduced. Moreover, 0.4% and 2.0% ripasudil reduced CD45<sup>+</sup>-infiltrated leukocyte frequency, <i>Cd11b</i> and <i>Cd11c</i> mRNA levels, and the frequencies of mature dendritic cells, IFNγ-, and IL-17- producing CD4<sup>+</sup>T cells in the dLNs of recipients. Re-epithelization rate of the grafted cornea was significantly higher in the 0.4% and 2.0% ripasudil groups than in the control. Topically applied ripasudil prolonged graft survival by downregulating neovascularization and inflammation factors, while promoting corneal re-epithelization, suggesting that ripasudil may be useful for suppressing immunological rejection in corneal transplantation.

scholarly journals TCT-157 Increased Local Cytokine Production at Culprit Superficial Femoral Artery Plaques

2012 ◽  
Vol 60 (17) ◽  
pp. B45-B46
Author(s):  
Cameron Donaldson ◽  
David Schneider ◽  
Daniel Bertges ◽  
Julie Adams ◽  
Nader Elgharib ◽  
...  
Keyword(s):  
Femoral Artery ◽  
Superficial Femoral Artery ◽  
Cytokine Production ◽  
Local Cytokine

Exacerbates kidney injury in mice subjected to sepsis: critical impact of used heparin

2018 ◽  
Vol 6 (4) ◽  
pp. 323-335
Author(s):  
Peter Weighardt ◽  
Niels Hayashi
Keyword(s):  
Oxidative Stress ◽  
Acute Kidney Injury ◽  
Cytokine Production ◽  
Multiorgan Failure ◽  
Kidney Injury ◽  
Tubular Dysfunction ◽  
Cytokine Storm ◽  
Peritubular Capillary ◽  
Inflammatory Reactions ◽  
Local Cytokine

Sepsis is the leading cause of death in critically ill patients, and the incidence of sepsis is increasing causes multiorgan failure, including acute kidney injury (AKI) and patients with both sepsis and AKI have an especially high mortality rate. Several different pathophysiological mechanisms have been proposed for sepsis-induced AKI: vasodilation-induced glomerular hypoperfusion, dysregulated circulation within the peritubular capillary network, inflammatory reactions by systemic cytokine storm or local cytokine production, and tubular dysfunction induced by oxidative stress animal sepsis models have been developed using LPS infusion. Renal dysfunction evaluated by serum creatinine and BUN was found in acute non-survivors (<24 hours) and decreased urine output in subacute non-survivors (24–96 hours). The study show that increased AKI in animal with blood collected heparin (P=0.002) compared to citrate, furthermore; sham mice that received heparin did not develop AKI.

scholarly journals Combined effects of IL-12 and IL-18 on the clinical course and local cytokine production in murine pulmonary infection withCryptococcus neoformans

1999 ◽  
Vol 29 (2) ◽  
pp. 643-649 ◽  
Author(s):  
Mahboob Hossain Qureshi ◽  
Tiantuo Zhang ◽  
Yoshinobu Koguchi ◽  
Kazutoshi Nakashima ◽  
Haruki Okamura ◽  
...  
Keyword(s):  
Pulmonary Infection ◽  
Cytokine Production ◽  
Clinical Course ◽  
Combined Effects ◽  
Local Cytokine

Epithelial Herpetic Simplex Keratitis Recurrence and Graft Survival After Corneal Transplantation in Patients With and Without Atopy

2007 ◽  
Vol 143 (4) ◽  
pp. 623-628.e1 ◽  
Author(s):  
Renata A. Rezende ◽  
Tiago Bisol ◽  
Kristin Hammersmith ◽  
Ana Luisa Hofling-Lima ◽  
Guy F. Webster ◽  
...  
Keyword(s):  
Graft Survival ◽  
Corneal Transplantation

scholarly journals Corneal graft survival analysis after penetrating keratoplasty in a retrospective cohort study

2021 ◽  
pp. 12-20
Author(s):  
B.E. Malyugin ◽  
◽  
S.N. Sakhnov ◽  
V.V. Myasnikova ◽  
A.V. Klokov ◽  
...  
Keyword(s):  
Cohort Study ◽  
Survival Analysis ◽  
High Risk ◽  
Graft Survival ◽  
Retrospective Cohort Study ◽  
Retrospective Cohort ◽  
Penetrating Keratoplasty ◽  
Corneal Transplantation ◽  
Minimal Risk ◽  
General Group

Purpose. To assess the results of penetrating keratoplasty and identify the risk factors for the graft disease (GD) development. Material and methods. Data of 582 patients after corneal transplantation performed in the period since 2011 to 2019 for keratoconus (41%), as well for corneal leucorrhoea and dystrophies (59%), aggravated by concomitant pathology, the so-called high-risk keratoplasty (HRK) were analyzed in a retrospective cohort study. We estimated the functional results and incidence of GD. The calculation of the t-criterion; cross-tabulation method; Kaplan–Meier survival analysis and multivariate analysis were applied. Results. As a result of penetrating keratoplasty, the BCVA (best corrected visual acuity) in patients with keratoconus increased by 20%; in patients with HRK – by 8%. In the general group the graft survival rate was 72%, while the 8-year successful graft engraftment in patients with keratoconus comprised 91%, with HRK – 60%. A significant relationship of the GD development with preoperative diagnosis and rekeratoplasty was determined. The risk of GD incidence was minimal in patients under 30 years of age and maximum in patients aged from 50 to 70 years. Survival rates for corneal transplants were better in men than in women. Conclusion. Penetrating keratoplasty in patients with keratoconus provides a good functional result with a minimal risk of GD development. With penetrating keratoplasty in high-risk patients, the effectiveness of surgical interventions is significantly lower (by ~30%). Key words: keratoconus, penetrating keratoplasty, corneal transplantation, survival analysis

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