Dietary soy protein effects on disease and IGF-I in male and female Han:SPRD-cy rats

Parathyroid hormone (PTH) exerts both catabolic and anabolic actions on bone. Studies on the skeletal effects of PTH have seldom considered the effects of gender. Our study was designed to determine whether the response of mouse bone to PTH differed according to sex. As a first step, we analyzed gender differences with respect to bone mass and structural properties of 4 month old PTH treated (80 μg/kg per day for 2 weeks) male and female CD-1 mice. PTH significantly increased fat free weight/body weight, periosteal bone formation rate, mineral apposition rate, and endosteal single labeling surface, while significantly decreasing medullary area in male mice compared with vehicle treated controls, but induced no significant changes in female mice. We then analyzed the gender differences in bone marrow stromal cells (BMSC) isolated from 4 month old male and female CD-1 mice following treatment with PTH (80 μg/kg per day for 2 weeks). PTH significantly increased the osteogenic colony number and the alkaline phosphatase (ALP) activity (ALP/cell) by day 14 in cultures of BMSCs from male and female mice. PTH also increased the mRNA level of receptor activator of nuclear factor κB ligand in the bone tissue (marrow removed) of both females and males. However, PTH increased the mRNA levels of IGF-I and IGF-IR only in the bones of male mice. Our results indicate that on balance a 2-weeks course of PTH is anabolic on cortical bone in this mouse strain. These effects are more evident in the male mouse. These differences between male and female mice may reflect the greater response to PTH of IGF-I and IGF-IR gene expression in males enhancing the anabolic effect on cortical bone.

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Expression patterns and immunolocalisation of IGF-I and IGF-II in male and female gonads of the Neotropical characid fish Astyanax fasciatus

Fish Physiology and Biochemistry ◽

10.1007/s10695-018-0550-9 ◽

2018 ◽

Vol 45 (1) ◽

pp. 167-176

Author(s):

Paula Suzanna Prado ◽

Ana Paula Barbosa Pinheiro ◽

André Alberto Weber ◽

Nilo Bazzoli ◽

Elizete Rizzo

Keyword(s):

Expression Patterns ◽

Male And Female ◽

Astyanax Fasciatus ◽

Igf I

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Transgenic Mice Overexpressing Des-Acyl Ghrelin Show Small Phenotype

Endocrinology ◽

10.1210/en.2004-0629 ◽

2005 ◽

Vol 146 (1) ◽

pp. 355-364 ◽

Cited By ~ 99

Author(s):

Hiroyuki Ariyasu ◽

Kazuhiko Takaya ◽

Hiroshi Iwakura ◽

Hiroshi Hosoda ◽

Takashi Akamizu ◽

...

Keyword(s):

Transgenic Mice ◽

Biological Effects ◽

Lower Body ◽

Acid Modification ◽

Male And Female ◽

Body Weights ◽

Igf I ◽

Actin Promoter ◽

Acyl Ghrelin ◽

Serum Gh

Ghrelin, a 28-amino acid acylated peptide, displays strong GH-releasing activity in concert with GHRH. The fatty acid modification of ghrelin is essential for the actions, and des-acyl ghrelin, which lacks the modification, has been assumed to be devoid of biological effects. Some recent reports, however, indicate that des-acyl ghrelin has effects on cell proliferation and survival. In the present study, we generated two lines of transgenic mice bearing the preproghrelin gene under the control of chicken β-actin promoter. Transgenic mice overexpressed des-acyl ghrelin in a wide variety of tissues, and plasma des-acyl ghrelin levels reached 10- and 44-fold of those in control mice. They exhibited lower body weights and shorter nose-to-anus lengths, compared with control mice. The serum GH levels tended to be lower, and the serum IGF-I levels were significantly lower in both male and female transgenic mice than control mice. The responses of GH to administered GHRH were normal, whereas those to administered ghrelin were reduced, especially in female transgenic mice, compared with control mice. These data suggest that overexpressed des-acyl ghrelin may modulate the GH-IGF-I axis and result in small phenotype in transgenic mice.

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Circulating IGF-I in plasma of growing male and female turkeys of medium and heavy weight lines

Domestic Animal Endocrinology ◽

10.1016/0739-7240(93)90031-6 ◽

1993 ◽

Vol 10 (4) ◽

pp. 267-277 ◽

Cited By ~ 12

Author(s):

W.L. Bacon ◽

K.E. Nestor ◽

D.A. Emmerson ◽

R. Vasilatos-Younken ◽

D.W. Long

Keyword(s):

Male And Female ◽

Heavy Weight ◽

Igf I

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Altered IGF-I and IGFBPs in Senescent Male and Female Rats

The Journals of Gerontology Series A ◽

10.1093/gerona/54.3.b111 ◽

1999 ◽

Vol 54 (3) ◽

pp. B111-B115 ◽

Cited By ~ 20

Author(s):

S. Severgnini ◽

D. T. Lowenthal ◽

W. J. Millard ◽

F. A. Simmen ◽

B. H. Pollock ◽

...

Keyword(s):

Female Rats ◽

Male And Female ◽

Male And Female Rats ◽

Igf I

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SH2-B Is Required for Both Male and Female Reproduction

Molecular and Cellular Biology ◽

10.1128/mcb.22.9.3066-3077.2002 ◽

2002 ◽

Vol 22 (9) ◽

pp. 3066-3077 ◽

Cited By ~ 49

Author(s):

Satoshi Ohtsuka ◽

Satoshi Takaki ◽

Masanori Iseki ◽

Kanta Miyoshi ◽

Naomi Nakagata ◽

...

Keyword(s):

Growth Factors ◽

Follicle Stimulating Hormone ◽

Critical Role ◽

Cumulus Cells ◽

Female Reproduction ◽

Targeted Disruption ◽

Male And Female ◽

Factor I ◽

Igf I

ABSTRACT Many growth factors and hormones modulate the reproductive status in mammals. Among these, insulin and insulin-like growth factor I (IGF-I) regulate the development of gonadal tissues. SH2-B has been shown to interact with insulin and IGF-I receptors, although the role of SH2-B in these signals has not been clarified. To investigate the role of SH2-B, we generated mice with a targeted disruption of the SH2-B gene. Both male and female SH2-B−/− mice showed slight retardation in growth and impaired fertility. Female knockout mice possess small, anovulatory ovaries with reduced numbers of follicles and male SH2-B−/− mice have small testes with a reduced number of sperm. SH2-B−/− cumulus cells do not respond to either follicle-stimulating hormone or IGF-I. These data suggest that SH2-B plays a critical role in the IGF-I-mediated reproductive pathway in mice.

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Intestinotrophic effects of exogenous IGF-I are not diminished in IGF binding protein-5 knockout mice

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00903.2006 ◽

2007 ◽

Vol 292 (6) ◽

pp. R2144-R2150 ◽

Cited By ~ 7

Author(s):

Sangita G. Murali ◽

Xiaowen Liu ◽

David W. Nelson ◽

Angela K. Hull ◽

Michael Grahn ◽

...

Keyword(s):

Body Weight ◽

Binding Protein ◽

Wild Type ◽

Intestinal Growth ◽

Male And Female ◽

Igf I ◽

Igf Binding ◽

Igf Binding Protein ◽

Stimulation Of ◽

Igfbp 3

IGF binding protein-5 (IGFBP-5) modulates the availability of IGF-I to its receptor and potentiates the intestinotrophic action of IGF-I. Our aim was to test the hypothesis that stimulation of intestinal growth due to coinfusion of IGF-I with total parenteral nutrition (TPN) solution is dependent on increased expression of IGFBP-5 through conducting our studies in IGFBP-5 knockout (KO) mice. IGFBP-5 KO, heterozygote (HT) and wild type (WT) male and female mice were maintained with TPN or TPN plus coinfusion of IGF-I [recombinant human (rh)IGF-I; 2.5 mg·kg−1·day−1] for 5 days. The concentration of IGF-I in serum was 73% greater ( P < 0.0001) in mice given TPN + IGF-I infusion compared with TPN alone. IGF-I attenuated the 2–3 g loss of body weight associated with TPN in WT mice, whereas KO and HT mice did not show improvement in body weight with IGF-I treatment. KO and HT mice had significantly greater levels of circulating IGF-I binding proteins (IGFBPs) compared with WT mice. Intestinal growth due to IGF-I was observed in all groups treated with IGF-I based on greater concentrations of protein and DNA in small intestine and colon and significantly greater crypt depth and muscularis thickness in jejunum. Jejunal expression of IGFBP-5 mRNA was greater in WT mice, whereas IGFBP-3 mRNA was greater in KO mice treated with IGF-I. In summary, the absence of the IGFBP-5 gene did not block the ability of IGF-I to stimulate intestinal growth, possibly because greater jejunal expression of IGFBP-3 compensates for the absence of IGFBP-5.

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Differential expression of IGF-I mRNA and peptide in the male and female gonad during early development of a bony fish, the tilapia Oreochromis niloticus

General and Comparative Endocrinology ◽

10.1016/j.ygcen.2005.11.008 ◽

2006 ◽

Vol 146 (3) ◽

pp. 204-210 ◽

Cited By ~ 59

Author(s):

Giorgi Berishvili ◽

Helena D’Cotta ◽

Jean-François Baroiller ◽

Helmut Segner ◽

Manfred Reinecke

Keyword(s):

Differential Expression ◽

Early Development ◽

Oreochromis Niloticus ◽

Bony Fish ◽

Female Gonad ◽

Male And Female ◽

Igf I

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Effect of diabetes mellitus on levels of insulin-like growth factors and their carrier proteins in Guerin’s carcinoma and it’s perifocal tissue in rats

Research n Practical Medicine Journal ◽

10.17709/2410-1893-2021-8-4-4 ◽

2021 ◽

Vol 8 (4) ◽

pp. 44-52

Author(s):

E. M. Frantsiyants ◽

V. A. Bandovkina ◽

I. V. Kaplieva ◽

E. I. Surikova ◽

I. V. Neskubina ◽

...

Keyword(s):

Diabetes Mellitus ◽

Growth Factors ◽

Growth Dynamics ◽

Female Rats ◽

Carrier Proteins ◽

Male And Female ◽

Male And Female Rats ◽

Igf I ◽

Guerin’S Carcinoma ◽

Insulin Like Growth Factors

Purpose of the study. Diabetes mellitus (DM) is considered an independent risk factor for higher cancer incidence and death rates. The system of insulin-like growth factors and their carrier proteins (IGF and IGFBP) and hyperglycemia create favorable conditions for the proliferation and metastasis of cancer cells.Materials and methods. Outbred male and female rats were divided into groups (n = 8 each): controls - with Guerin's carcinoma; main group - Guerin's carcinoma growing in presence of DM. Experimental DM was reproduces in animals by the single intraperitoneal alloxan injection (150 mg/kg body weight). After 10 days of the carcinoma growth, levels of IGF and IGFBP in the tumor and in it's perifocal area were measured using ELISA.Results. DM in females upregulated levels of glucose both in the tumor and in perifocal tissues by 1.8 (p < 0.05) and 8.1 times, respectively, but caused opposite changes in IGF-I - it's increase by 6.3 times in the tumor and decrease by 3.2 times in the perifocal area; as a result, such tumors with small primary nodes were more "aggressive" and actively metastasized. In males, induced DM downregulated levels of glucose, IGF-II and IGFBP2 in the carcinoma by 8.4, 3.1 and 1.7 (p < 0.05) times, respectively, and increased levels of IGF-I and IGFBP2 by 1.4 and 1.3 times (p < 0.05) in the perifocal area without changing glucose levels; as a result, tumor volumes exceeded the values in the standard growth, without metastasizing into visceral organs.Conclusion. We revealed gender differences in changing levels of glucose and IGF both in the tumor and in it's perifocal tissue in rats with Guerin's carcinoma growing in presence of DM; these differences could determine different tumor growth dynamics in male and female rats.

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Feeding soy protein isolate and treatment with estradiol have different effects on mammary gland morphology and gene expression in weanling male and female rats

Physiological Genomics ◽

10.1152/physiolgenomics.00096.2013 ◽

2013 ◽

Vol 45 (22) ◽

pp. 1072-1083 ◽

Cited By ~ 9

Author(s):

Isabelle R. Miousse ◽

Neha Sharma ◽

Michael Blackburn ◽

Jamie Vantrease ◽

Horacio Gomez-Acevedo ◽

...

Keyword(s):

Mammary Gland ◽

Soy Protein ◽

Serum Insulin ◽

Soy Protein Isolate ◽

Protein Isolate ◽

Female Rats ◽

Sprague Dawley ◽

Male And Female ◽

Receptor Modulator ◽

Male And Female Rats

Isoflavones are phytochemical components of soy diets that bind weakly to estrogen receptors (ERs). To study potential estrogen-like actions of soy in the mammary gland during early development, we fed weanling male and female Sprague-Dawley rats a semipurified diet with casein as the sole protein source from postnatal day 21 to 33, the same diet substituting soy protein isolate (SPI) for casein, or the casein diet supplemented with estradiol (E2) at 10 μg/kg/day. In contrast to E2, the SPI diet induced no significant change in mammary morphology. In males, there were 34 genes for which expression was changed ≥2-fold in the SPI group vs. 509 changed significantly by E2, and 8 vs. 174 genes in females. Nearly half of SPI-responsive genes in males were also E2 responsive, including adipogenic genes. Serum insulin was found to be decreased by the SPI diet in males. SPI and E2 both downregulated the expression of ERα ( Esr1) in males and females, and ERβ ( Esr2) only in males. Chromatin immunoprecipitation revealed an increased binding of ERα to the promoter of the progesterone receptor ( Pgr) and Esr1 in both SPI- and E2-treated males compared with the casein group but differential recruitment of ERβ. ER promoter binding did not correlate with differences in Pgr mRNA expression. This suggests that SPI fails to recruit appropriate co-activators at E2-inducible genes. Our results indicate that SPI behaves like a selective estrogen receptor modulator rather than a weak estrogen in the developing mammary gland.

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