Improvement of platelet storage conditions by using new polyolefin containers

Transfusion ◽  
1997 ◽  
Vol 37 (5) ◽  
pp. 476-481 ◽  
Author(s):  
J Wildt-Eggen ◽  
JG Schrijver ◽  
HJ Bouter-Valk ◽  
R Fijnheer ◽  
M Bins ◽  
...  
Keyword(s):  
Storage Conditions ◽  
Platelet Storage

scholarly journals Platelet storage and functional integrity

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Gianmatteo Vit ◽  
Harald Klüter ◽  
Patrick Wuchter
Keyword(s):  
Platelet Transfusion ◽  
Structural Characteristics ◽  
Storage Conditions ◽  
Transfusion Practice ◽  
Common Interest ◽  
Laboratory Staff ◽  
German Medical Association ◽  
Platelet Storage ◽  
Technological Advances ◽  
Assessment Procedures

AbstractPlatelet transfusion is a topic of common interest for many specialists involved in patient care, from laboratory staff to clinical physicians. Various aspects make this type of transfusion different from those of other blood components. In this review, the challenges in platelet transfusion practice that are relevant for laboratory colleagues will be discussed, highlighting how the biochemical and structural characteristics of these blood elements directly affect their function and consequently the clinical outcome. More than 1,300 platelet concentrates are transfused in Germany every day, and several types are offered by their respective manufacturers. We describe the technological advances in platelet concentrate production, with a focus on how the storage conditions of platelets can be improved. Laboratory quality assessment procedures for a safe transfusion are discussed in detail. For this purpose, we will refer to the Hemotherapy Directives (Richtlinie Hämotherapie) of the German Medical Association.

Transfer of arachidonic acid from phosphatidylcholine to phosphatidylethanolamine during storage of human platelets for 5 days

1990 ◽  
Vol 68 (1) ◽  
pp. 117-122 ◽  
Author(s):  
Julie Lacasse ◽  
Rosalind S. Labow ◽  
Morris Kates ◽  
George A. Adams
Keyword(s):  
Arachidonic Acid ◽  
Storage Conditions ◽  
Phospholipid Metabolism ◽  
Human Platelets ◽  
Acyl Transfer ◽  
Acid Uptake ◽  
Molar Composition ◽  
Platelet Storage ◽  
Head Groups ◽  
Glycerol Uptake

Human platelets are routinely stored for 5 days prior to transfusion, but they deteriorate during storage. Since very little information is available concerning the effect of storage on platelet phospholipid metabolism, the biosynthesis and remodelling of platelet phospholipids were studied. Platelets were incubated separately with [14C]glycerol, [14C]arachidonic acid, or a mixture of [14C]glycerol and [3H]arachidonic acid, and stored in a platelet storage medium at 22 °C. Maximum glycerol uptake (20%) was attained after 6 h. [14C]Glycerol was incorporated into phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol, and to a much lesser extent phosphatidylserine, under storage conditions for 5 days. The distribution of the initial arachidonic acid uptake was not as would be expected based on the molar composition of endogenous phospholipids. The arachidonic acid (75%) which was taken up within 10 min of incubation distributed 55% into the phosphatidylcholine and only 14% into the phosphatidylethanolamine; the molar composition is actually 18% phosphatidylcholine and 47% phosphatidylethanolamine. During storage, there was a continuous transfer of the radiolabeled arachidonic acid from phosphatidylcholine to phosphatidylethanolamine until, after 5 days, the distribution of arachidonic acid was identical to the endogenous distribution. In contrast, no change in the glycerol incorporation pattern was detected during storage. This suggested that the mechanism for arachidonic acid redistribution was not through exchange of polar head groups, but through acyl transfer of arachidonic acid from phosphatidylcholine to phosphatidylethanolamine.Key words: human, platelet, storage, arachidonate, phospholipids.

In Vivo Evaluation of Extended Stored Platelet Concentrates.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 946-946 ◽  
Author(s):  
Sherrill J. Slichter ◽  
Doug Bolgiano ◽  
Jill Corson ◽  
Mary Kay Jones ◽  
Todd Christoffel ◽  
...  
Keyword(s):  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Storage Conditions ◽  
Mean Value ◽  
Platelet Concentrates ◽  
In Vivo Evaluation ◽  
Platelet Storage ◽  
Volunteer Donor ◽  
Simple Ratio

Abstract Background: With the introduction of bacterial testing, extended storage of platelets is now possible as long as platelet viability is maintained. Although apheresis platelets have recently been licensed for seven days of storage, platelet concentrates are still only licensed for five days. We performed studies to determine the post-storage viability of platelets stored as concentrates. As yet, there are no established criteria for platelet viability that must be met at the end of storage. However, two different criteria for evaluating post-storage platelet viability have been suggested: compare the stored platelet results with each donor’s fresh platelet results; or establish a fixed platelet standard with an expected mean value along with the lower limit of the 95% confidence interval (lower 95% CI) of the mean. Methods: Forty-four normal volunteers donated a unit of whole blood, the whole blood was centrifuged to give platelet-rich plasma (PRP), and the PRP was then centrifuged to produce a platelet concentrate. The platelet concentrates were re-suspended and stored in either 100% plasma or in 20% plasma and 80% Plasmalyte (a platelet storage solution) for five to eight days in Terumo Teruflex bags. At the end of storage, a sample of blood was obtained from each volunteer to prepare fresh platelets to compare the results of a fresh platelet transfusion with the transfusion of the same volunteer’s stored platelets. The fresh and stored platelets were alternately radiolabeled with either 51Cr or 111In, re-injected into their volunteer donor, and serial blood samples were drawn from the volunteer after transfusion of the fresh and stored platelets to determine platelet recoveries and survivals. Results: Results are shown in Table 1. Conclusions: After seven days of storage in plasma, the platelets could qualify for standards of: 74% and 53% as a simple ratio of fresh to stored platelet recoveries and survivals, respectively; according to Dumont’s method of analysis (Transfusion, in press), achievable stored platelet recoveries would be 63% of fresh and survivals would be 40% of fresh; and as a fixed standard, platelet recoveries averaged 45% and survivals 4.4 days with lower 95% CI’s of 35% and 3.3 days, respectively. After eight days of storage in plasma, platelet recoveries and survivals gave unacceptable results. Instead of increasing or at least maintaining post-storage platelet viability, Plasmalyte markedly reduced platelet viability compared to plasma stored platelets at each storage interval. In Vivo Radiolabeled Autologous Platelet Recoveries and Survivals STORAGE CONDITIONS PLATELET RECOVERIES (%) PLATELET SURVIVALS (Days) Solution Time (Days) N Fresh Stored Fresh Stored Data are given as the average ±1 S.D. Plasma 5 10 62±12 55±11 7.7±1.5 6.1±1.0 Plasma 6 10 61±7 46±12 8.2±1.5 5.1±1.6 Plasmalyte 6 3 47±30 29±16 5.1±3.4 1.8±0.2 Plasma 7 10 61±11 45±14 8.3±1.0 4.4±1.5 Plasmalyte 7 5 62±17 30±12 7.4±0.6 2.2±1.1 Plasma 8 3 67±12 31±8 8.3±0.6 2.9±0.8 Plasmalyte 8 3 70±3 21±8 8.2±0.8 0.7±0.1

Co-Culture of Acinetobacter calcoaceticus-baumannii complex and Staphylococcus saprophyticus Supports Simple Point Contamination Model in Recent Cases of Transfusion-Related Sepsis

2020 ◽  
Vol 154 (Supplement_1) ◽  
pp. S14-S14
Author(s):  
Christopher Kerantzas ◽  
Jacob Merwede ◽  
Edward Snyder ◽  
Jeanne Hendrickson ◽  
Christopher Tormey ◽  
...  
Keyword(s):  
Acinetobacter Calcoaceticus ◽  
Storage Conditions ◽  
Luria Bertani ◽  
Growth Media ◽  
Columbia Blood Agar ◽  
Staphylococcus Saprophyticus ◽  
Platelet Storage ◽  
Solid Media ◽  
Apheresis Platelets ◽  
Transfusion Reactions

Abstract The CDC recently reported a series of four septic transfusion reactions across three states resulting from contamination of apheresis platelet products. The apheresis platelets were contaminated with strains of both Acinetobacter calcoaceticus-baumannii complex (ACBC) and Staphylococcus saprophyticus (Ss). Two of the reported septic transfusion reactions occurred at our institution. The CDC investigation showed that isolates of each species were genetically related and suggested a point source of contamination. However, the contamination of blood products with ACBC is rare and the co-occurrence of these two species in all four cases was unusual. We hypothesized that there was an augmentative interaction between the clinical isolates of ACBC and Ss from these cases that contributed to their repeated co-occurrence. To test this hypothesis, we compared the growth characteristics of ACBC and Ss when cultured together versus independently. We used isolates from the contaminated platelets for our studies and performed experiments using both solid and liquid growth media. Experiments on solid media assessed density of growth and macroscopic morphology after cross-streaking on Columbia Blood Agar (CBA) and Luria-Bertani (LB) agar. Experiments in liquid media assessed growth by CFU counts in LB broth, platelet-poor plasma, and apheresis platelets. Growth in apheresis platelets was performed using standard, room temperature blood bank platelet storage conditions. Results of these experiments showed a higher CFU concentration of Ss when co-cultured with ACBC in LB broth only after several days, as compared to Ss alone under the same conditions. Otherwise, there was no evidence of augmented growth by either CFU concentration or growth rate in LB broth, plasma, or platelets at other time points. Similarly, there was no evidence of augmented growth by colony density or morphology when cross-streaking strains on either CBA or LB agar. As a result, we conclude that the co-occurrence of these two species in platelets is likely a coincidence of the point contamination suggested by the CDC investigation and not the result of growth augmentation between the two species.

Staphylococcus epidermidis forms biofilms under simulated platelet storage conditions

Transfusion ◽  
2007 ◽  
Vol 47 (7) ◽  
pp. 1143-1153 ◽  
Author(s):  
Carey Greco ◽  
Irene Martincic ◽  
Arjeta Gusinjac ◽  
Miloslav Kalab ◽  
Ann-Fook Yang ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Storage Conditions ◽  
Platelet Storage

A Review Study of Dendrimer Nanoparticles Influences on Stored Platelet in Order to Treat Patients (2001-2020)

2021 ◽  
Vol 17 ◽  
Author(s):  
Tahereh Zadeh Mehrizi ◽  
Mehdi Shafiee Ardestani ◽  
Sedigheh Amini Kafiabad
Keyword(s):  
Molecular Weight ◽  
Storage Time ◽  
Storage Temperature ◽  
Storage Conditions ◽  
Normal Function ◽  
Platelet Storage ◽  
Review Study ◽  
And Function ◽  
Positively Charged

Background: Platelets are sensitive to chilling, therefore, the optimal storage temperature for maintaining normal function and structure in platelets is 22-24 °C up to 3-5 days. Introduction: Platelets are important blood cells involved in immunity, inflammation, and thrombosis. Today, platelet products are widely used to prevent bleeding in patients with thrombocytopenia and coagulopathy disorders. As a result, maintaining the quality of these products is very important. Method: In this review study, the reported influences of various dendrimers on platelets from 2001 to 2020 were investigated. Result: The results showed that positively charged dendrimers could cause platelet aggregation and activation during platelet storage time through their amine residues. In addition to surface charge, high generations, molecular weight and concentration are not recommended in the field of platelet storage and treatment. In contrast, negatively charged dendrimers, usually used at lower generations with proper molecular weight, lower size (less than 100 nm) and their carboxyl residues, cannot induce adverse effects on platelets during storage time. In addition, the results of this study revealed that PEGylation of dendrimers and platelets could improve platelet storage conditions. Conclusion: As anionic dendrimers can improve platelet storage time without inducing significant changes in morphology and function of platelets, they are recommended in the field of platelet storage and treatment.

scholarly journals Influence of Oxidative Stress on Stored Platelets

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
K. Manasa ◽  
R. Vani
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Storage Conditions ◽  
Oxygen Species ◽  
Defense System ◽  
Platelet Storage ◽  
Storage Solutions ◽  
Insight Into

Platelet storage and its availability for transfusion are limited to 5-6 days. Oxidative stress (OS) is one of the causes for reduced efficacy and shelf-life of platelets. The studies on platelet storage have focused on improving the storage conditions by altering platelet storage solutions, temperature, and materials. Nevertheless, the role of OS on platelet survival during storage is still unclear. Hence, this study was conducted to investigate the influence of storage on platelets. Platelets were stored for 12 days at 22°C. OS markers such as aggregation, superoxides, reactive oxygen species, glucose, pH, lipid peroxidation, protein oxidation, and antioxidant enzymes were assessed. OS increased during storage as indicated by increments in aggregation, superoxides, pH, conjugate dienes, and superoxide dismutase and decrements in glucose and catalase. Thus, platelets could endure OS till 6 days during storage, due to the antioxidant defense system. An evident increase in OS was observed from day 8 of storage, which can diminish the platelet efficacy. The present study provides an insight into the gradual changes occurring during platelet storage. This lays the foundation towards new possibilities of employing various antioxidants as additives in storage solutions.

Novel platelet storage conditions

2014 ◽  
Vol 21 (6) ◽  
pp. 491-496 ◽  
Author(s):  
Kelley E. Capocelli ◽  
Larry J. Dumont
Keyword(s):  
Storage Conditions ◽  
Platelet Storage
Sign in / Sign up

Export Citation Format

Share Document