scholarly journals Harmless treatment method of dead chickens in scale chicken farm

2021 ◽  
Vol 245 ◽  
pp. 02008
Author(s):  
Chunlin Yu ◽  
Bo Xia ◽  
Mohan Qiu ◽  
Longhuan Du ◽  
Zengrong Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Treatment Method ◽  
Poultry Production ◽  
Calcium Hypochlorite ◽  
Chicken Farm ◽  
Practical Guidance ◽  
And Staphylococcus Aureus ◽  
Total Bacteria ◽  
Better Than

The harmless treatment of dead chickens is an important content in poultry production. This paper compared the effects of the harmless treatment of dead chickens by adding different disinfectants, including quicklime and calcium hypochlorite. The results showed that both quicklime and calcium hypochlorite could increase the total bacteria content, improve the harmless treatment capacity, and shorten the killing time of Escherichia coli and Staphylococcus aureus. The effect of quicklime was better than that of calcium hypochlorite. The experiment provided practical guidance for harmless treatment of scale chicken farm.

Persistent contamination of Salmonella, Campylobacter, Escherichia coli, and Staphylococcus aureus at a broiler farm in New Zealand

2020 ◽  
Vol 66 (3) ◽  
pp. 171-185 ◽  
Author(s):  
Kristine Castañeda-Gulla ◽  
Evelyn Sattlegger ◽  
Anthony N. Mutukumira
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Quantitative Polymerase Chain Reaction ◽  
Control Measures ◽  
Poultry Production ◽  
Chain Reaction ◽  
Pathogen Control ◽  
Broiler Farm ◽  
Polymerase Chain ◽  
And Staphylococcus Aureus

Intensive poultry production due to public demand raises the risk of contamination, creating potential foodborne hazards to consumers. The prevalence and microbial load of the pathogens Campylobacter, Salmonella, Staphylococcus aureus, and Escherichia coli was determined by standard methods at the farm level. After disinfection, swab samples collected from wall crevices, drinkers, and vents were heavily contaminated, as accumulated organic matter and dust likely protected the pathogens from the disinfectants used. The annex floor also showed high microbial concentrations, suggesting the introduction of pathogens from external environments, highlighting the importance of erecting hygiene barriers at the entrance of the main shed. Therefore, pathogen control measures and proper application of disinfectants are recommended as intervention strategies. Additionally, quantitative polymerase chain reaction (qPCR) was evaluated as a quantification tool. qPCR showed limitations with samples containing low microbial counts because of the low detection limit of the method. Thus, bacterial pre-enrichment of test samples may be necessary to improve the detection of pathogens by qPCR.

Preparation, Characterization and Antibacterial Activity of Chitosan Schiffbases

2011 ◽  
Vol 287-290 ◽  
pp. 1947-1951 ◽  
Author(s):  
Jing Xian Xu ◽  
Ye Ting Lin ◽  
Qiang Lin ◽  
Wen Yuan ◽  
Xue Qiong Yin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Schiff Bases ◽  
Optical Density ◽  
Elemental Analysis ◽  
Antibacterial Activities ◽  
Cp Mas Nmr ◽  
And Staphylococcus Aureus ◽  
Better Than

Two Schiff bases of chitosan (CTS) were synthesized from 4-methoxylbenzylaldehyde (CH3O-CTS) and 4-methylbenzylaldehyde(CH3-CTS). The Schiff bases were characterized by FTIR, DSC-TGA, solid13C CP-Mas NMR, and elemental analysis. Antibacterial activities of the Schiff bases against Escherichia coli and Staphylococcus aureus were measured by the optical density method. The antibacterial activity of the Schiff bases is better than that of the original CTS. The IC50of CH3O-CTS and CH3-CTS againstEscherichia coliis respectively 40.3 ppm and 43 ppm, which being 38.5 ppm and 39.5 ppm against Staphylococcus aureus, lower than IC50of chitosan, being 59.5 ppm and 52 ppm againstEscherichia coliand Staphylococcus aureus, respectively.

scholarly journals Inhibitory effect and disinfectant activity of Syzygium aromaticum L. and Ocimum gratissimum L. essential oils against Escherichia coli and Staphylococcus aureus isolated from sheep carcasses

2019 ◽  
Vol 11 ◽  
pp. 1-12
Author(s):  
Larissa de Oliveira ◽  
Sabrina de Souza Sales
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Essential Oils ◽  
Inhibitory Effect ◽  
Syzygium Aromaticum ◽  
Ocimum Gratissimum ◽  
Broth Macrodilution ◽  
And Staphylococcus Aureus ◽  
Suspension Test ◽  
Better Than

Syzygium aromaticum L. and Ocimum gratissimum L. essential oils were tested for their effectiveness in reduction the counts of inoculated Staphylococcus aureus and Escherichia coli in sheepmeat. The inhibitory effect was analysed by disk diffusion and broth macrodilution method with four strains and seven concentrations. Disinfectant activity of the oils was assessed using the suspension test with two strains and two concentrations. The inhibition was observed at concentrations 400, 200 and 100 μL/mL. Syzygium aromaticum L. oil was better than Ocimum gratissimum L. against bacteria isolated from sheep carcasses. The minimum bactericidal concentration of the essential oils aginst inocula ranged from 50 to 200 μL/mL. The suspension test showed that after 5 minutes of contact, Syzygium aromaticum L. disinfectant at 400 μL/mL deactivated the inoculants completely. The results demonstrated that essential oils exerted a significant bactericidal and bacteriostatic action against pathogens.

scholarly journals Perbedaan Daya Hambat Bakteri dari Propolis Cair yang Ada di Pasaran Terhadap Escherichia Coli dan Staphylococcus Aureus Secara In Vitro

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Bangun Azhari Yusuf ◽  
Aziz Djamal ◽  
Asterina Asterina
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Market Research ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Breeding Location ◽  
Main Component ◽  
And Staphylococcus Aureus ◽  
Better Than

Abstrak Propolis adalah zat yang digunakan lebah melindungi sarangnya dari berbagai ancaman. Komponen utama propolis adalah resin yang dikumpulkan lebah dan dicampur dengan air liurnya. Manusia juga menggunakan propolis sebagai obat berbagai penyakit, seperti infeksi bakteri. Propolis memiliki kemampuan antibakteri terhadap Escherichiacoli dan Staphylococcus aureus. Kualitas dan jenis propolis sesuai dengan komposisi kimia, iklim dan tempat berkembang biak. Tujuan penelitian ini adalah melihat perbedaan daya hambat bakteri Propolis cair 1 dan Propolis cair 2 yang ada di pasaran terhadap Escherichia coli dan Staphylococcus aureus secara in vitro. Propolis yang akan diuji adalah propolis cair yang ada di pasaran dipilih dua secara acak. Penelitian dilaksanakan pada bulan Januari 2013 di laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas. Jenis penelitian eksperimentalmenggunakan metode difusi (metode cakram) dan analitik dengan menghubungkan perbedaan kedua propolis sebagai antibakteri. Hasil penelitian ini menunjukkan propolis cair 1 dan propolis cair 2 yang ada di pasaran tidak memiliki efek antibakteri terhadap Escherichia coli dan terdapat perbedaan daya hambat bakteri dari kedua propolisterhadap Staphylococcus aureus. Propolis cair 2 (diklaim berasal dari Selandia baru) memiliki daya hambat bakteri yang lebih baik daripada propolis cair 1 (diklaim berasal dari Malaysia).Kata kunci: propolis cair, perbedaan daya hambat bakteri, Escherichia coli, Staphylococcus aureusAbstract Propolis is substance which use to protect bee hive from various threat. The main component of propolis is resin which is collected and mixed with saliva. The human also use propolis as a treatment against various illness such as bacterial infection. Propolis has an antibacterial effect to Escherichia coli and Stahpylococcus aureus. The quality and type of propolis is also in accordance with chemical composition, climate and their breeding location. The objective of this study was to find out the differentiation of the antibacterial effect of Propolis 1 and Propolis 2 in the market to Escherichia coli and Staphylococcus aureus by in vitro. Propolis to be tested for this study is random liquid propolis in the market. Research was conducted in January 2013 in the laboratory of Microbiology Faculty of Medicine, University of Andalas. This type of study was the experimental diffusion method (disc method) and the analytic distinction owned by linking two of propolis as an antibacterial effect. These results indicate that propolis 1 and propolis 2 have no ability antibacterial effect to Escherichia coli, and both of propolis have differences antibacterial effect to Staphylococcus aureus. Propolis 2 (from New Zealand claimed) has antibacterial effect better than propolis 1 (from Malaysia claimed).Keywords: propolis, differentiation of the antibacterial effect, Escherichia coli, Staphylococcus aureus

Comparison of the Effects of Silver in Nanostructured and Ultrahigh Diluted Form on Growth and Volatile Compounds Produced by Escherichia coli and Staphylococcus aureus

2020 ◽  
Vol 10 (3) ◽  
pp. 316-329
Author(s):  
Fateme Mirzajani ◽  
Amin Hamidi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Life Cycles ◽  
Gas Chromatography Mass Spectrometry ◽  
Untreated Sample ◽  
E Coli ◽  
Volatile Metabolites ◽  
Octyl Acetate ◽  
Volatile Organic ◽  
And Staphylococcus Aureus

Introduction: In this project, the growth and volatile metabolites profiles of Escherichia coli (E. coli ) and Staphylococcus aureus were monitored under the influence of silver base chemical, nanoparticle and ultra-highly diluted compounds. Materials & Methods: The treatments were done for 12000 life cycles using silver nanoparticles (AgNPs) as well as ultra-highly diluted Argentum nitricum (Arg-n). Volatile organic metabolites analysis was performed using gas chromatography mass spectrometry (GC-MS). The results indicated that AgNPs treatment made the bacteria resistant and adapted to growth in the nanoparticle condition. The use of ultra-highly diluted Arg-n initially increased growth but it decreased later. Also, with the continuous usage of these materials, no more bacterial growth was observed. Results: The most important compounds produced by E. coli are Acetophenone, Octyl acetate, Styrene, 1,8-cineole, 4-t-butyl-2-(1-methyl-2-nitroethyl)cyclohexane, hexadecane and 2-Undecanol. The main compounds derived from S. aureus are Acetophenone,1,8-cineole, Benzaldehyde, 2-Hexan-1-ol, Tridecanol, Dimethyl Octenal and tetradecane. Acetophenone and 1,8-cineole were common and produced by both organisms. Conclusion: Based on the origin of the produced volatiles, main volatiles percentage of untreated sample is hydrocarbon (>50%), while bacteria treatments convert the ratio in to aldehydes, ketones and alcohols in the case of AgNPs, (>80%) and aldehydes, ketones and terpenes in the case of Arg-n (>70%).

Penggunaan Limbah Logam Tembaga yang Didaur Ulang untuk Antibakteri dan Degradasi Metil Merah Secara Fotolisis

2019 ◽  
Vol 4 (1) ◽  
pp. 15
Author(s):  
Ariyetti Ariyetti ◽  
Muhammad Nasir ◽  
Safni Safni ◽  
Syukri Darajat
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nitrate ◽  
Copper Sulfate ◽  
Bacterial Growth ◽  
Methyl Red ◽  
Copper Metal ◽  
Sulfate Hydrate ◽  
Red Dye ◽  
And Staphylococcus Aureus

<p><em>Metil merah merupakan salah satu zat warna golongan azo yang sering digunakan dalam industri dan laboratorium. Penggunaan metil merah dapat menimbulkan efek terhadap kesehatan dan lingkungan. Oleh sebab itu dilakukan metode fotodegradasi dengan menggunakan semikonduktor dan radiasi sinar tampak. Semikonduktor yang digunakan yaitu berbahan dasar tembaga sulfat hidrat dan perak nitrat. Prekusor tembaga sulfat hidrat dibuat dari pengolahan limbah logam tembaga hasil pemotongan tembaga yang ada di bengkel Lembaga Ilmu Pengetahuan Indonesia (LIPI) Bandung. Bahan semikonduktor juga memiliki kemampuan dalam menghambat pertumbuhan bakteri. Hasil optimum yang didapatkan dalam proses fotodegradasi dan antibakteri merupakan gabungan antara kedua prekusor tembaga sulfat hidrat dan perak nitrat dengan bantuan penyinaran. Kemampuan dalam menghambat pertumbuhan bakteri didapatkan persentase kematian 100 % untuk masing-masing bakteri, yaitu Escherichia coli dan Staphylococcus aureus. Aktifitas fotokatalitiknya dengan konsentrasi semikonduktor 10 ppm untuk mendegradasi zat warna metil merah 5 ppm, selama 23 jam, dimana persentase degradasi yang didapatkan dengan penyinaran lebih tinggi dibandingkan dengan tanpa penyinaran. Pengaruh pH larutan terhadap degradasi metil merah yaitu optimum pada pH 12 (basa).</em></p><p><em><br /></em></p><p><em>Methyl red is one of the azo group dyes that is often used in industry and laboratories. The use of methyl red can have an effect on health and the environment. Therefore photodegradation method is done by using semiconductor and visible light radiation. The semiconductor used is based on copper sulfate hydrate and silver nitrate. The copper sulphate hydrate precursor is made from the processing of copper-cut copper metal waste in the workshop of the Indonesian Institute of Sciences (LIPI) in Bandung. Semiconductor materials also have the ability to inhibit bacterial growth. The optimum results obtained in the photodegradation and antibacterial process are a combination of both copper sulfate hydrate precursor and silver nitrate with the help of irradiation. The ability to inhibit bacterial growth obtained 100% mortality for each bacterium, namely Escherichia coli and Staphylococcus aureus. Photocatalytic activity with 10 ppm semiconductor concentration to degrade methyl red dye 5 ppm, for 23 hours, where the percentage of degradation obtained by irradiation is higher than without irradiation. The effect of pH of the solution on the degradation of methyl red is optimum at pH 12 (base).</em></p>

scholarly journals Sulfaquinoxaline Oxidation and Toxicity Reduction by Photo-Fenton Process

2021 ◽  
Vol 18 (3) ◽  
pp. 1005
Author(s):  
Vanessa Ribeiro Urbano ◽  
Milena Guedes Maniero ◽  
José Roberto Guimarães ◽  
Luis J. del Valle ◽  
Montserrat Pérez-Moya
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pilot Plant ◽  
Environmental Water ◽  
Fenton Process ◽  
Experimental Conditions ◽  
Laboratory Bench ◽  
Degradation Rates ◽  
Bacterial Growth Inhibition ◽  
And Staphylococcus Aureus

Sulfaquinoxaline (SQX) has been detected in environmental water samples, where its side effects are still unknown. To the best of our knowledge, its oxidation by Fenton and photo-Fenton processes has not been previously reported. In this study, SQX oxidation, mineralization, and toxicity (Escherichia coli and Staphylococcus aureus bacteria) were evaluated at two different setups: laboratory bench (2 L) and pilot plant (15 L). The experimental design was used to assess the influence of the presence or absence of radiation source, as well as different H2O2 concentrations (94.1 to 261.9 mg L−1). The experimental conditions of both setups were: SQX = 25 mg L−1, Fe(II) = 10 mg L−1, pH 2.8 ± 0.1. Fenton and photo-Fenton were suitable for SQX oxidation and experiments resulted in higher SQX mineralization than reported in the literature. For both setups, the best process was the photo-Fenton (178.0 mg L−1 H2O2), for which over 90% of SQX was removed, over 50% mineralization, and bacterial growth inhibition less than 13%. In both set-ups, the presence or absence of radiation was equally important for sulfaquinoxaline oxidation; however, the degradation rates at the pilot plant were between two to four times higher than the obtained at the laboratory bench.

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