scholarly journals Decolourization of chicken compost derived liquid fertilizer via synergic ultraviolet (UV) irradiation and ozonation for enhanced microalgae cultivation

2021 ◽  
Vol 287 ◽  
pp. 04013
Author(s):  
Yik Lam Kam ◽  
Man Kee Lam ◽  
Yoke Wang Cheng ◽  
Yaleeni Kanna Dasan ◽  
Sie Yon Lau ◽  
...  
Keyword(s):  
Growth Rate ◽  
Uv Irradiation ◽  
Specific Growth ◽  
Biomass Productivity ◽  
Dry Weight ◽  
Optimal Parameters ◽  
Microalgae Cultivation ◽  
Liquid Fertilizer ◽  
Decolorization Efficiency ◽  
Initial Ph

Compost-derived liquid fertilizers are uncostly and nutrient-enriched; however, its dark brown appearance limits light uptake of microalgae during autotrophic cultivation. Here, integrated UV irradiation/ozonation pretreatment was employed to decolourize the compost solution prior to microalgae cultivation. Aforesaid pretreatment could accomplish 16.52 % (8 h) or 40.88 % (24 h) decolorization efficiency by using optimal parameters (initial pH of 12, ozone concentration of 30 mg/L, and ozone flow rate of 3 L/min. Compared to untreated compost solution, microalgae Chlorella vulgaris grew better in the medium supplemented with decolourized compost solution (after 24 h UV irradiation/ozonation). For the autotrophic cultivation of C. vulgaris with 10 vol.% compost solution, UV irradiation/ozonation pretreatment eventually increases the microalgae dry weight, specific growth rate, and biomass productivity from 0.58 g/L, 0.14 d-1, and 0.040 g/(L·d) to 0.88 g/L, 0.19 d-1, and 0.065 g/(L·d), respectively. Furthermore, the lipid content of microalgae has been increased by 33.33% with pretreatment of compost solution.

Evaluation of the Growth Performance of Microalgae Based on Fine pH Changes

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Filali R ◽  
◽  
Tian H ◽  
Micheils E ◽  
Taidi B ◽  
...  
Keyword(s):  
Growth Rate ◽  
Carbon Content ◽  
Specific Growth ◽  
Optimal Growth ◽  
Microalgae Cultivation ◽  
Photoautotrophic Growth ◽  
Microalgae Culture ◽  
Potential Applications ◽  
Photosynthetic Microorganisms ◽  
Range Of Values

Microalgae are photosynthetic microorganisms with many potential applications in the food, cosmetics, pharmaceutical and environmental industries. Currently, commercial microalgae production remains limited. Therefore, improving the growth and the culture density of the microalgae cultivation is one of the key enablers to open the way to mass production and commercialisation of these microorganisms. The effect of culture pH on the photoautotrophic growth of C. vulgaris over a large range of values has been investigated in pH-regulated cultures. For each microalgae culture, the specific growth rate, the cell density, the chlorophyll content, the intracellular carbon content and the nitrogen source consumption were monitored. Optimal growth and carbon incorporation have been observed at pH of 7.0. The fastest growth rate and highest biomass production of C. vulgaris were 0.074 h-1 and 0.896 g/L respectively. Under these conditions, a maximum carbon content of cells was 49 % (w/w).

scholarly journals Pengaruh Penambahan Pupuk Padat dan Cair terhadap Pertumbuhan, Jumlah Klorofil dan Kadar Protein Caulerpa racemosa, J.Agardh, 1873 (Ulvophyceae : Caulerpaceae)

2019 ◽  
Vol 8 (3) ◽  
pp. 269-276
Author(s):  
Vicky Dimas Pradhika ◽  
Suryono Suryono ◽  
Sri Sedjati
Keyword(s):  
Growth Rate ◽  
Protein Content ◽  
Chlorophyll Content ◽  
Specific Growth ◽  
Fertilizer Application ◽  
Average Weight ◽  
Caulerpa Racemosa ◽  
Daily Growth ◽  
Liquid Fertilizer ◽  
Wet Weight

Caulerpa racemosa merupakan salah satu jenis rumput laut hijau yang dapat hidup di daerah pasang surutmaupun daerah yang tenang/bebas dari pasang surut. Setiap tahun permintaan C. racemosa di Jepara terus meningkat. Usaha pengembangan budidaya C. racemosa perlu dilakukan supaya penyediaan kebutuhan pasar tidak bergantung pada alam. Upaya peningkatan produksi budidaya dapat dilakukan dengan penambahan nutrien di tambak budidaya. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian pupuk padat dan cair terhadap pertumbuhan (berat basah, laju pertumbuhan harian) dan kandungan klorofil serta kadar protein rumput laut C. racemosa. Perlakuan yang pada penelitian ini adalahpenambahan pupuk :A (pupuk padat), B (pupuk cair), C (pupuk padat dan cair). Pencapaian berat rata rata rumput laut sebagai berikut: Kontrol= 39,5 ± 6,36 gram; A= 11,5 ± 2,12 gram; B= 18,5 ± 10,6 gram; D= 52,6± 14,19 gram. Laju pertumbuhan spesifik C. racemosa adalah: A= 1,2 ± 0,37%; B= -1,64 ± 0,51%; C= -0,53 ± 0,89%; C= 1,7 ± 0,63%. Kandungan klorofil a C. racemosa pada minggu 6 yaitu: Kontrol= 0,022mg/gram; A=0,015 mg/gram; B= 0,017 mg/gram; C= 0,080 mg/gram. Kandungan klorofil b C. racemosa pada minggu ke 6 yaitu: A= 0,016 mg/gram; B= 0,018 mg/gram; C= 0,013 mg/gram; D=0,026. Kandungan protein sebelum pemberian perlakuan adalah 0,60 % sedangkan setelah diberiperlakuan yaitu: Kontrol= 0,70% ± 0,06; A= 0,75% ± 0,11; B= 0,60% ± 0,10; C= 0,73% ± 0,08. Hasil penelitian ini menunjukkan bahwa pemberian pupuk padat dan cair berpengaruh nyata pada berat basah dan laju pertumbuhan spesifik C. racemosa (p < 0,01) sedangkan pemberian pupuk tidak bepengaruh nyata pada kandungan klorofil dan kadar protein (p > 0,05).  Caulerpa racemosa is a type of green algae that can live’s in tidal areas and calm / free from tides. Every year the demand for C. racemosa in Jepara is continues to increase. Cultivation of C. racemosa need to be done so the market suply is not dependent only on nature. Efforts to increase culture production can be done by adding nutrients to aquaculture ponds. This study aims to determine the effect of solid and liquid fertilizer on growth (wet weight, daily growth rate), chlorophyll content and protein content of C. racemosa seaweed. Treathments of this study is fertilize additon: A (solid fertilizer), B (liquid fertilizer), C (solid and liquid fertilizer). The average weight of seaweed for 42 days is as follows: Control = 39.5 ± 6.36 grams; A = 11.5 ± 2.12 grams; B = 18.5 ± 10.6 grams; C = 52.6 ± 14.19 grams. Specific growth rates of C. racemosa produced for 42 days is: Control = 1.2 ± 0.37%; A = -1.64 ± 0.51%; B = -0.53 ± 0.89%; C = 1.7 ± 0.63%. C. racemosa’s Chlorophyll a content at week 6 is: Control = 0.022 mg/gram; A = 0.015 mg/gram; B = 0.017 mg/gram; C = 0.080 mg/gram. C. racemosa’s Chlorophyll b content at week 6 is: Control = 0.016 mg/gram; A = 0.018 mg/gram; B = 0.013 mg/gram; C = 0.026 mg/gram. Protein content before administration of treatment is 0.60% while after treatment, is: Control = 0.70% ± 0.06; A = 0.75% ± 0.11; B = 0.60% ± 0.10; C = 0.73% ± 0.08. The results of this study showed, the addition of solid and liquid fertilizers had a significant effect on wet weight and specific growth rate of C. racemosa (p <0.01) while fertilizer application did not significantly affect chlorophyll content and protein content (p> 0.05).

scholarly journals High-Throughput Optimization of Effective Microbes Nutrient Formulation via Automated Media Optimization System (AMOS)

2019 ◽  
Vol 7 (4.14) ◽  
pp. 1
Author(s):  
A I Saharuddin ◽  
N Z M N Rashid ◽  
N S Mohd Noor ◽  
N S Abdul Rahman ◽  
A Ismail ◽  
...  
Keyword(s):  
Growth Rate ◽  
Yeast Extract ◽  
Photosynthetic Bacteria ◽  
Animal Feed ◽  
Biomass Productivity ◽  
Dry Weight ◽  
Throughput Optimization ◽  
Beef Extract ◽  
Microwell Plate ◽  
Mrs Medium

Fortification of effective microbes (EM) such as Pediococcus acidilactici and photosynthetic bacteria in fertilizer and animal feed exhibiting effective outcomes in enhancing crops and livestock production as well as upholds sustainable agriculture concept. However, the productivity of the EM biomass stocks is unsurpassed to the demand due to non-optimized media. In this study, two sets of nutrient pair comprising of beef extract-manganese and yeast extract-glucose for P. acidilactici and photosynthetic bacteria respectively were studied. The optimum media formulated was based on the highest growth rate in 96-microwell plate system were subsequently tested in 1 L cultures volume for EM biomass production. Results showed that growth rate and dry weight of P. acidilactici in media supplemented with 64 g/L beef extract (0.145 h-1, 0.243 g) and 0.28 g/L manganese (0.1134 h-1, 0.32 g) were higher compared to control medium with an increment of 17.39 % and 54.59 % biomass productivity as compared to MRS medium respectively. Meanwhile, the growth rate and dry weight for Photosynthetic bacteria in media supplemented with 6.63 g/L glucose (0.2538 h-1, 0.167 g) and 0.44 g/L yeast extract (0.2733 h-1, 0.163 g) recorded 11.3 % and 8.73 % biomass productivity increment in comparison to the HCH medium.  

scholarly journals Poly(3-Hydroxybutyrate) Synthesis by Recombinant Escherichia coli arcA Mutants in Microaerobiosis

2006 ◽  
Vol 72 (4) ◽  
pp. 2614-2620 ◽  
Author(s):  
Pablo I. Nikel ◽  
M. Julia Pettinari ◽  
Miguel A. Galvagno ◽  
Beatriz S. Méndez
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Specific Growth Rate ◽  
Deletion Mutant ◽  
Specific Growth ◽  
Dry Weight ◽  
Low Oxygen ◽  
Oxygen Conditions ◽  
In The Wild ◽  
Microaerobic Conditions

ABSTRACT We assessed the effects of different arcA mutations on poly(3-hydroxybutyrate) (PHB) synthesis in recombinant Escherichia coli strains carrying the pha synthesis genes from Azotobacter sp. strain FA8. The arcA mutations used were an internal deletion and the arcA2 allele, a leaky mutation for some of the characteristics of the Arc phenotype which confers high respiratory capacity. PHB synthesis was not detected in the wild-type strain in shaken flask cultures under low-oxygen conditions, while ArcA mutants gave rise to polymer accumulation of up to 24% of their cell dry weight. When grown under microaerobic conditions in a bioreactor, the arcA deletion mutant reached a PHB content of 27% ± 2%. Under the same conditions, higher biomass and PHB concentrations were observed for the strain bearing the arcA2 allele, resulting in a PHB content of 35% ± 3%. This strain grew in a simple medium at a specific growth rate of 0.69 ± 0.07 h−1, whereas the deletion mutant needed several nutritional additives and showed a specific growth rate of 0.56 ± 0.06 h−1. The results presented here suggest that arcA mutations could play a role in heterologous PHB synthesis in microaerobiosis.

scholarly journals Cellular Microcystin Content in N-Limited Microcystis aeruginosa Can Be Predicted from Growth Rate

2001 ◽  
Vol 67 (1) ◽  
pp. 278-283 ◽  
Author(s):  
Benedict M. Long ◽  
Gary J. Jones ◽  
Philip T. Orr
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Cell Volume ◽  
Microcystis Aeruginosa ◽  
Specific Growth ◽  
Weight Ratio ◽  
Dry Weight ◽  
Cell Protein ◽  
Protein Ratio ◽  
Cell Quota

ABSTRACT Cell quotas of microcystin (Q MCYST; femtomoles of MCYST per cell), protein, and chlorophyll a(Chl a), cell dry weight, and cell volume were measured over a range of growth rates in N-limited chemostat cultures of the toxic cyanobacterium Microcystis aeruginosa MASH 01-A19. There was a positive linear relationship betweenQ MCYST and specific growth rate (μ), from which we propose a generalized model that enablesQ MCYST at any nutrient-limited growth rate to be predicted based on a single batch culture experiment. The model predicts Q MCYST from μ, μmax(maximum specific growth rate), Q MCYSTmax(maximum cell quota), and Q MCYSTmin (minimum cell quota). Under the conditions examined in this study, we predict aQ MCYSTmax of 0.129 fmol cell−1 at μmax and a Q MCYSTmin of 0.050 fmol cell−1 at μ = 0. Net MCYST production rate (R MCYST) asymptotes to zero at μ = 0 and reaches a maximum of 0.155 fmol cell−1 day−1at μmax. MCYST/dry weight ratio (milligrams per gram [dry weight]) increased linearly with μ, whereas the MCYST/protein ratio reached a maximum at intermediate μ. In contrast, the MCYST/Chla ratio remained constant. Cell volume correlated negatively with μ, leading to an increase in intracellular MCYST concentration at high μ. Taken together, our results show that fast-growing cells of N-limited M. aeruginosa are smaller, are of lower mass, and have a higher intracellular MCYST quota and concentration than slow-growing cells. The data also highlight the importance of determining cell MCYST quotas, as potentially confusing interpretations can arise from determining MCYST content as a ratio to other cell components.

scholarly journals Effect of the Carbon Concentration, Blend Concentration, and Renewal Rate in the Growth Kinetic ofChlorellasp.

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Adriano Arruda Henrard ◽  
Gabriel Martins da Rosa ◽  
Luiza Moraes ◽  
Michele Greque de Morais ◽  
Jorge Alberto Vieira Costa
Keyword(s):  
Specific Growth ◽  
Biomass Productivity ◽  
Cost Effective ◽  
Batch Cultivation ◽  
Maximum Specific Growth Rate ◽  
Residual Water ◽  
Microalgae Cultivation ◽  
Renewal Rate ◽  
Alternative Sources ◽  
Semicontinuous Cultivation

The microalgae cultivation can be used as alternative sources of food, in agriculture, residual water treatment, and biofuels production. Semicontinuous cultivation is little studied but is more cost-effective than the discontinuous (batch) cultivation. In the semicontinuous cultivation, the microalga is maintained in better concentration of nutrients and the photoinhibition by excessive cell is reduced. Thus, biomass productivity and biocompounds of interest, such as lipid productivity, may be higher than in batch cultivation. The objective of this study was to examine the influence of blend concentration, medium renewal rate, and concentration of sodium bicarbonate on the growth ofChlorellasp. during semicontinuous cultivation. The cultivation was carried out in Raceway type bioreactors of 6 L, for 40 d at 30°C, 41.6 µmol m−2 s−1, and a 12 h light/dark photoperiod. Maximum specific growth rate (0.149 d−1) and generating biomass (2.89 g L−1) were obtained when the blend concentration was 0.80 g L−1, the medium renewal rate was 40%, and NaHCO3was 1.60 g L−1. The average productivity (0.091 g L−1 d−1) was achieved with 0.8 g L−1of blend concentration and NaHCO3concentration of 1.6 g L−1, independent of the medium renewal rate.

THE KINETICS OF METABOLISM OF GIBBERELLA FUJIKUROI IN STIRRED CULTURE

1964 ◽  
Vol 10 (3) ◽  
pp. 407-444 ◽  
Author(s):  
A. Borrow ◽  
Sheila Brown ◽  
E. G. Jefferys ◽  
R. H. J. Kessell ◽  
Eithne C. Lloyd ◽  
...  
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Nitrogen Uptake ◽  
Exponential Growth ◽  
Ammonium Acetate ◽  
Specific Growth ◽  
Dry Weight ◽  
Gibberella Fujikuroi ◽  
Ammonium Tartrate ◽  
Kinetics Of

Some aspects are described of the kinetics of the growth of Gibberella fujikuroi in nitrogen-limited media containing either ammonium nitrate, ammonium acetate, ammonium tartrate, urea, or glycine. Also varied were inoculum size, agitation rate, pH, and initial concentrations of glucose and nitrogen source. The significance of kinetic parameters used in this, and published studies, is discussed.A lag phase was only found on ammonium acetate media or when high concentrations of glucose were present. Early growth was exponential on all nitrogen sources. On ammonium acetate the specific growth rate decreased at a dry weight of ca. 1 mg/g WS (Whole unfiltered Sample). On ammonium nitrate, early exponential growth utilized more NH3-nitrogen than NO3-nitrogen with a concomitant decrease in pH. In the range pH 3.0–2.8 NH3-nitrogen uptake and dry weight increase ceased, but NO3-nitrogen uptake continued, and the pH increased until growth and NH3-nitrogen uptake restarted. This pattern could be repeated. Finally, exponential growth was resumed at a low specific growth rate. On glycine, urea, and ammonium tartrate media, exponential growth continued to a dry weight of about 7 mg/g WS. During this period the uptakes relative to dry weight (contributions) of glucose, nitrogen, phosphate, and magnesium remained constant and were unaffected by the rate of agitation, as also was the specific growth rate, but the latter decreased with increasing glucose concentration.A period of linear growth could follow the exponential period. The contribution of glucose was greater, and that of phosphate and magnesium less, than during exponential growth. The dry weight at which exponential growth changed to linear growth was greater the higher the rate of agitation, and this change may be a response to oxygen restriction.After nitrogen exhaustion, fat and carbohydrate accumulation in the cells largely accounted for the increase in dry weight. The specific rates of dry weight increase and glucose uptake remained constant over the lower range of initial nitrogen concentrations. Both rates decreased with increasing nitrogen over the higher range.Gibberellic acid production began at, or soon after, nitrogen exhaustion. The amount present increased linearly with time. The productivity decreased with increasing glucose concentration, and first increased and then decreased with increasing initial nitrogen. The maximum amount produced was proportional to the initial nitrogen provided. Some published results are discussed in the light of these relations.

scholarly journals Effect of soil extracts concentration on specific growth rate and lipid content of chlorella vulgaris in bolds basal medium

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Ani Idris ◽  
M. Atta ◽  
A. Bukhari
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Chlorella Vulgaris ◽  
Mass Culture ◽  
Specific Growth ◽  
Basal Medium ◽  
Dry Weight ◽  
Soil Extract ◽  
Soil Extracts ◽  
Key Factor

Effective nutrient medium is the key factor that significantly influences the specific growth rate and the final concentration of microalgae. The main objective of this study was to optimize the microalgal growth in mass culture system under the effect of soil extract in modified Bolds Basal medium. Chlorella vulgaris was grown aseptically for 10 days at five different concentrations of soil extract (10, 20, 30, 40, 50ml/L) at 25°C cultivation temperature. After 10 days of cultivation, Chlorella vulgaris showed maximum cell concentration of 5x107/ml at 30ml/L of soil extract concentration which corresponds to the maximum specific growth rate 1.56μd-1 with the doubling rate of 2.25d-1   andlipid yield of 22.74 % of dry weight.________________________________________GRAPHICAL ABSTRACT

scholarly journals Influence of Culture Conditions and Medium Compositions on the Production of Bacteriocin-Like Inhibitory Substances by Lactococcus lactis Gh1

2020 ◽  
Vol 8 (10) ◽  
pp. 1454
Author(s):  
Roslina Jawan ◽  
Sahar Abbasiliasi ◽  
Joo Shun Tan ◽  
Shuhaimi Mustafa ◽  
Murni Halim ◽  
...  
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Lactococcus Lactis ◽  
Organic Nitrogen ◽  
Specific Growth ◽  
Cell Concentration ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Initial Ph

Antibacterial peptides or bacteriocins produced by many strains of lactic acid bacteria have been used as food preservatives for many years without any known adverse effects. Bacteriocin titres can be modified by altering the physiological and nutritional factors of the producing bacterium to improve the production in terms of yield and productivity. The effects of culture conditions (initial pH, inoculum age and inoculum size) and medium compositions (organic and inorganic nitrogen sources; carbon sources) were assessed for the production of bacteriocin-like inhibitory substances (BLIS) by Lactococcus lactis Gh1 in shake flask cultures. An inoculum of the mid-exponential phase culture at 1% (v/v) was the optimal age and size, while initial pH of culture media at alkaline and acidic state did not show a significant impact on BLIS secretion. Organic nitrogen sources were more favourable for BLIS production compared to inorganic sources. Production of BLIS by L. lactis Gh1 in soytone was 1.28-times higher as compared to that of organic nitrogen sources ((NH4)2SO4). The highest cell concentration (XmX = 0.69 ± 0.026 g·L−1) and specific growth rate (μmax = 0.14 h−1) were also observed in cultivation using soytone. By replacing carbon sources with fructose, BLIS production was increased up to 34.94% compared to BHI medium, which gave the biomass cell concentration and specific growth rate of 0.66 ± 0.002 g·L−1 and 0.11 h−1, respectively. It can be concluded that the fermentation factors have pronounced influences on the growth of L. lactis Gh1 and BLIS production. Results from this study could be used for subsequent application in process design and optimisation for improving BLIS production by L. lactis Gh1 at larger scale.

On-line viable biomass measurement and estimation of the specific growth rate of activated sludge from municipal wastewater treatment

2001 ◽  
Vol 43 (7) ◽  
pp. 97-102 ◽  
Author(s):  
E. J. November ◽  
J. F. Van Impe
Keyword(s):  
Growth Rate ◽  
Activated Sludge ◽  
Specific Growth Rate ◽  
Municipal Wastewater ◽  
Specific Growth ◽  
Dry Weight ◽  
Municipal Wastewater Treatment ◽  
Experimental Conditions ◽  
On Line ◽  
Viable Biomass

In order to control wastewater processes, on-line measurements of important process variables are crucial. This contribution focuses on the applicability of the Biomass Monitor for on-line viable biomass measurement of activated sludge from a municipal wastewater plant. In addition, the specific growth rate of the sludge is estimated on-line, based on the information derived from the device under study. Compared to dry weight measurements, the Biomass Monitor hardware offers the advantage of a biologically more appropriate observation of the biomass by only taking into account the viable cells in the population. The optimal measurement frequency of the biomass monitoring device for the given experimental conditions has been determined. Furthermore, the capacitance readings have been correlated with off-line analyses of dry weight of the sludge during the experimental phase in which no death of cells occurred. Finally, an evaluation of the estimator of the specific growth rate including its tuning is presented.

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