This study aimed to isolate indigenous Antagonistic Bacteria (AB) against common soil-borne phytopathogens, including Rhizoctonia solani, Phythium sp., Fusarium oxysporum. Biosynthesis of Indole-3-Acetic Acid (IAA), generation of Hydrogen Cyanide (HCN), and siderophores production were assessed for their involvement in the antagonistic activities. Rhizospheric soil of bean roots, sunflowers, wheat, rice, and humic and semi flooded soils were used to isolate twenty-one bacterial strains for phytopathogenic antagonism. It was found that nine isolates have potential antagonistic activity against three common soilborne pathogens. Antifungal productivity for IAA, HCN, and siderophores was screened on the isolates while nine ABs were identified. To choose statistically significant isolate for the formulation, the principal component analysis was performed with five variables (IAA production, siderophores production index, antagonistic activities against three phytopathogens). Among the nine isolates, the isolate Pseudomonas alicaligenes shew a positive correlation with all variables. In particular, the strain demonstrated to be an antagonistic strain against the fungal pathogens and a strong producer of siderophores, HCN and IAA, We prepared the biocontrol agents with rice flour, glutinous rice flour, Monosodium Glutamate and Chitosan that were found to maintain the up to three months for convenience of field applications.