Regression of Schistosoma mansoni associated morbidity among Ugandan preschool children following praziquantel treatment: A randomised trial

Preschool children suffer from morbidity attributable to Schistosoma mansoni. We compared a single and double dose of praziquantel treatment on the regression of S. mansoni associated morbidity in children less than six years in Uganda. We measured the sizes of spleen and liver as well as liver fibrosis before treatment and 8 months after treatment among children who either received one dose (n = 201) or two doses (n = 184) of praziquantel (standard oral dose of 40 mg/kg body weight). Heamoglobin measurements were also taken. Overall, liver enlargement reduced from 52.2% (95% CI (Confidence interval) 45.1, 59.3) to 17.9% (95% CI 12.9, 23.9) with a single dose and from 48.4 (95% CI 40.9, 55.8) to 17.9% (95% CI 12.7, 24.3) with a double dose and there was no significant difference between the changes in proportion of children with enlarged liver between the two treatment groups. The proportion of children with enlarged spleen was not significantly reduced in the group treated with either one or two doses, 47.8% (95% CI 41.7, 54.9) to 45.3% (95% CI 38.3, 52.4) and 48.4% (95% CI 40.9,55.8) to 40.8% 95% CI 33.6, 48.2), respectively. Liver fibrosis detected among children getting single dose (n = 9) or double doses (n = 13) resolved after treatment with praziquantel. The number of children with low heamoglobin significantly reduced from 51.2% (95% CI 44.1, 58.3) to 0.5% (0.2, 0.8) and 61.4% (95% CI 53.9,68.5) to 1.1% (95% CI 0.1, 3.9) after single and double dose treatment, respectively. These results suggest that there is no evidence of a difference in effect between one dose of praziquantel and two doses in reversing morbidity attributable to S. mansoni among children less than six years of age.

Elys deficiency constrains Kras-driven tumour burden by amplifying oncogenic stress

ABSTRACTThe nucleoporin ELYS, encoded by AHCTF1, is a large multifunctional protein with essential roles in nuclear pore assembly and mitosis. Using a zebrafish model of hepatocellular carcinoma, in which the expression of an inducible mutant kras transgene (krasG12V) drives hepatocyte-specific hyperplasia and liver enlargement, we show that reducing ahctf1 gene dosage by 50% markedly shrinks tumour burden, while non-hyperplastic tissues are unaffected. We demonstrate that ahctf1 heterozygosity impairs nuclear pore formation, mitotic spindle assembly and chromosome segregation, leading to DNA damage and activation of TP53-dependent and independent mechanisms of cell death and cell cycle arrest. This selective vulnerability of cancer cells to mild disruption of Elys function uncovers a novel synthetic lethal interaction between ahctf1 and kras mutations that could be exploited therapeutically. Heterozygous expression of both ahctf1 and ranbp2, or treatment of heterozygous ahctf1 larvae with the nucleocytoplasmic transport inhibitor, Selinexor, completely blocked krasG12V-driven hepatocyte hyperplasia, revealing promising avenues for combinatorial treatments.

METHOD OF TELEPORTATION BASED ON NATURE-LIKE USING OF BIOLOGICAL OBJECTS

SEFER hA MINAGIM, p. 574 (photo 1), on behalf of an older book, writes that according to Jewish tradition, the navel does not close completely after childbirth, but remains partially open to extract various infections from the body. "From the "greenness" called "gable- zaht" (דזשאָנדַאַס איז קרענק די – disease jaundice), he will take a male pigeon for a man, and a dove for a woman; and put it on the navel, and the pigeon (dove), having drained all the infection from it (the patient), will die. Well-known veterinarian, Dr. Yekutiel Sharabi, owner of an elite veterinary clinic in Northern Tel Aviv, studied the causes of pigeon death after such a session. He found that immediately after treatment with pigeons, the patient's blood bilirubin level decreases by 25%, and recovery lasts an average of 3 days (as opposed to 28 days with conventional treatment). Autopsies of pigeons that died after the session showed liver enlargement and dysfunction. If the session was applied to a healthy person, not a single pigeon died. [1] In this paper, we consider a heuristic method for using quantum teleportation protocols in convergent dualuse biotechnologies based on new physical principles of operation [1-80]. The features of these biotechnologies will be considered within the framework of the possibilities of convalescence of pathological conditions (for example, nosological form: liver damage (lat. jecur, jecor, hepar, etc. - Greek ἧπαρ) by the hepatitis "C" virus (non-A, non-B hepatitis, or NANBH, or HCV infection). A hypothetical convalescence technology can be implemented by preparing cascades of entangled states of biological objects. In fact, this paper implies a certain information exchange in the planet's biogeosphere, hypotheses, protocols, and schemes for implementing these mechanisms based on new physical principles of action (NFP) [1-80].

Cucurbituril Ameliorates Liver Damage Induced by Microcystis aeruginosa in a Mouse Model

Microcystis aeruginosa is a cyanobacterium that produces a variety of cyclic heptapeptide toxins in freshwater. The protective effects of the macromolecular container cucurbit[7]uril (CB7) were evaluated using mouse models of cyanotoxin-induced liver damage. Biochemical analysis of liver function was performed to gauge the extent of liver damage after exposure to cyanobacterial crude extract [CCE; LD50 = 35 mg/kg body weight; intraperitoneal (i.p.)] in the absence or presence of CB7 (35 mg/kg body weight, i.p.). CCE injection resulted in liver enlargement, potentiated the activities of alanine aminotransferase (ALT) and glutathione S-transferase (GST), increased lipid peroxidation (LPO), and reduced protein phosphatase 1 (PP1) activity. CCE-induced liver enlargement, ALT and GST activities, and LPO were significantly reduced when CB7 was coadministered. Moreover, the CCE-induced decline of PP1 activity was also ameliorated in the presence of CB7. Treatment with CB7 alone did not affect liver function, which exhibited a dose tolerance of 100 mg/kg body wt. Overall, our results illustrated that the addition of CB7 significantly reduced CCE-induced hepatotoxicity (P < 0.05).

Environmental Exposure of Wild Carnivores to Zoonotic Pathogens: Leptospira Infection in the First Free Living Wolf (Canis lupus Linnaeus, 1758) Found Dead in the Friuli Venezia Giulia Region

Leptospirosis is a worldwide-spread zoonosis causing disease and death in dogs and in humans. A Leptospiral infection has been recorded in several wild carnivore species in Europe, but tissue pathological changes were not commonly described. The Grey wolf (Canis lupus) has been expanding its distribution range in north-eastern Italy during the last decade. A young wolf, representing the first individual handled in the region, was found road-killed and then submitted to necropsy. Pathological changes included erosive lesions of gingival mucosa, mild liver enlargement, and multifocal degenerative-necrotic areas along with hyperemic reactive lesions; multifocal interstitial nephritis and multifocal lung hemorrhages were observed. A Polymerase Chain Reaction (PCR) able to detect pathogenic species of Leptospira performed on a kidney sample was positive. Serological reactions for serogroup Gryppotyphosa (1:6400), Pomona (1:800), and Icterohaemorrhagiae (1:200) were evidenced by MAT. Genotyping by Multilocus Sequence Typing (MLST) performed on detected Leptospira characterized it as belonging to Sequence Type (ST) 117, which refers to L. kirschneri, serogroup Pomona, serovar Mozdok. Regardless of the role of Leptospira infection as an eventual predisposing factor to the road killing of this wolf, to the best of the authors’ knowledge, this is the first report of Leptospira-induced pathology in a wolf in Europe. Surveys on Leptospira infection in free-ranging wildlife species should be pursued in order to achieve further epidemiological knowledge on the circulation of the Leptospira strain.

METHOD OF TELEPORTATION BASED ON NATURE-LIKE USING OF BIOLOGICAL OBJECTS

SEFER hA MINAGIM, p. 574 (photo 1), on behalf of an older book, writes that according to Jewish tradition, the navel does not close completely after childbirth, but remains partially open to extract various infections from the body. "From the "greenness" called "gable- zaht" (די קרענק איז דזשאָנדאַס – disease jaundice), he will take a male pigeon for a man, and a dove for a woman; and put it on the navel, and the pigeon (dove), having drained all the infection from it (the patient), will die. Well-known veterinarian, Dr. Yekutiel Sharabi, owner of an elite veterinary clinic in Northern Tel Aviv, studied the causes of pigeon death after such a session. He found that immediately after treatment with pigeons, the patient's blood bilirubin level decreases by 25%, and recovery lasts an average of 3 days (as opposed to 28 days with conventional treatment). Autopsies of pigeons that died after the session showed liver enlargement and dysfunction. If the session was applied to a healthy person, not a single pigeon died. [1] In this paper, we consider a heuristic method for using quantum teleportation protocols in convergent dual-use biotechnologies based on new physical principles of operation [1-80]. The features of these biotechnologies will be considered within the framework of the possibilities of convalescence of pathological conditions (for example, nosological form: liver damage (lat. jecur, jecor, hepar, etc. Greek - ἧπαρ) by the hepatitis "C" virus (non-A, non-B hepatitis, or NANBH, or HCV infection). A hypothetical convalescence technology can be implemented by preparing cascades of entangled states of biological objects. In fact, this paper implies a certain information exchange in the planet's biogeosphere, hypotheses, protocols, and schemes for implementing these mechanisms based on new physical principles of action [1-80].

Demographic and clinical characteristics of dogs with centroblastic lymphoma

Background and Aim: Centroblastic lymphoma (CBL) is the most common morphological type of lymphoma found in dogs; it is usually identified through cytology in veterinary clinical practice. This study aimed to identify the demographic and clinical characteristics of dogs with CBL that was diagnosed with cytology and immunocytochemistry. Materials and Methods: Dogs with a suspicion of lymphoma were diagnosed by cytology supported by immunocytochemistry with the use of the updated Kiel classification adapted for dogs. During the analyzed time period, 336 lymphomas were diagnosed in dogs, including 171 cases of CBL. Epidemiological and clinical data from the dogs with CBL were provisionally collected. Results: The epidemiology analysis revealed an increased risk of CBL in Rottweilers, golden retrievers, and Bernese mountain dogs. At admission, most of the dogs displayed generalized lymphadenopathy with spleen and liver enlargement. The most common hematological abnormality was leukocytosis due to neutrophilia. The most common biochemical abnormality was elevated alanine aminotransferase and alkaline phosphatase activities and selective hypoproteinemia due to hypoalbuminemia. Conclusion: Rottweilers, Bernese mountain dogs, and golden retrievers appear to be overrepresented among dogs with CBL. CBL is usually diagnosed at an advanced clinical stage according to the World Health Organization; however, it is usually accompanied by only minor hematological and biochemical abnormalities.

Pathological features of cloned calves that died in the neonatal period

ABSTRACT: Somatic-cell nuclear transfer is a cloning technique that enables the creation of a viable embryo from a donor adult to produce a genetically identical individual. This technique opens numerous potential possibilities for medicine and animal reproduction. However, several reports have documented cloning-related issues. Embryo and fetal losses remain significantly higher than in other techniques, and there is a high incidence of dystocia and hydrops, which decreases efficiency and increases costs. Animals delivered at term often exhibit a syndrome known as macrosomia and experience difficulties in adapting to life outside the uterus, and death is a common outcome. In the present study, 41 cloned calves that died in the neonatal period were subjected to gross and histopathological examination. Most important gross lesions were found in the liver (enlargement, congestion, yellowish color), kidneys (brownish color at surface and cut, and cysts), lungs (atelectasis, parenchymal consolidation, and secretions in bronchi and bronchioles), and heart (concentric and eccentric hypertrophy, hematic cysts, persistence of ductus arteriosus). Primary microscopic findings were seen in the liver, kidneys, and lungs from neonatal calves. In the liver, 85% of the animals exhibited hepatic degeneration. The presence of a brownish pigment within the cortical tubules of the kidneys was found in approximately 90% of the samples; the presence of this pigment has not been previously reported in cloned calves. In the lungs, a large number of animals exhibiting lesions characteristic of pneumonia (55%). These changes were the pivotal causes of death, mainly due to problems in adapting to life outside the uterus and opportunistic infections in the neonatal period. Further investigation focusing on pathological anatomical changes is necessary to map these abnormalities in cloned animals.

Diagnosis of Pulmonary Hypertension Using Ultrasonography in the Management of Critical Patients in the Intensive Care Unit

Pulmonary arterial hypertension is most often diagnosed in its advanced stages because of the nonspecific nature of early symptoms and signs. Although clinical assessment is essential when evaluating patients with suspected pulmonary arterial hypertension, echocardiography is a key screening tool in the diagnostic algorithm. Echocardiography is valuable in assessing prognosis and treatment options, monitoring the efficacy of specific therapeutic interventions, and detecting the preclinical stages of disease. In this report, we reported A 35 years old woman admitted to the emergency unit with breathing difficulty which has felt since 2 days before. she transferred from rural hospital after hospitalized for a week. The patient has history of spinal surgery due to spondylitis tuberculosis 8 months ago and has an anti-tuberculosis drug for 2 months. The vital signs show respiratory failure with blood pressure 114/70 mmHg, heart rate 118 bpm, respiratory rate 35-40 bpm and pulse saturation 80-85% with non-rebreathing mask. Physical examination shows increasing work of breathing, without rales and wheezing, liver enlargement palpated 3 fingers below right ribs the chest x ray shows cardiomegaly and right pleural effusion