Pharmacognostic Screening and Anti-inflammatory Investigation of the Methanol extract of stem bark of Blighia unijugata Baker (Sapindaceae)

Introduction: Blighia unijugata is a potent medicinal plant extensively employed in traditional herbal remedies for the treatment of various diseases such as fever, fertility, inflammation, hypertension, migraine and treatment of infections due to microorganisms. Aim: This work, investigates the pharmacognostic screening and anti-inflammatory activities of the methanol extract of B. unijugata stem bark. Method: Collection, drying, pulverization, and methanol extraction of the stem bark were done accordingly. The screening of phytochemical constituents and Pharmacognostic numerical data were carried out. The chromatographic analysis was carried out using TLC. The acute toxicity was determined using Lorke’s method. Methanol extract was investigated for anti-inflammatory effect in albino rats using egg-induced hind paw oedema at doses of 200, 400 and 600mg/kg body weight respectively. Result: The macroscopical investigation showed the stem bark outer layer is greyish and, the inner layer is pale reddish brown, disagreeable odour, bitter, Microscopical screening revealed the presence of starch grains, trichomes and sclerenchyma cells. Chemomicroscopic result revealed the presence of lignin, starch, calcium oxalate, cellulose, stone cells. Qualitative phytochemical screening of the powdered bark showed the presence of saponins, steroids, tannins, and resins. Numerical data: moisture content/ weight loss on drying gave value of 7.7%, percentage yield of 6.7%, alcoholic soluble extractive 5.6%, water soluble extractive 6.3%, total ash of 7.1%, acid insoluble ash 0.57% and water soluble ash 4.56%. The chromatographic screening results were close compared with the standard drug. Toxicity test established the lethal dose of greater than 5000mg/kg. There was a significant inhibition of the edema p>0.05, the presence of various bioactive constituents may have contributed to the anti-inflammatory properties of the plant extract. Conclusion: The result of this study confirms that the barks of B. unijugata have anti-inflammatory effect and justifies the use as traditional treatment of inflammation and pain. Keywords: Blighia unijugata, Inflammation, Inflammatory agents, Anti inflammatory

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Effects of Methanol Extract of Piper chaba Stem Bark on Acute Inflammation in rats

Faridpur Medical College Journal ◽

10.3329/fmcj.v7i1.10294 ◽

2012 ◽

Vol 7 (1) ◽

pp. 26-28

Author(s):

F Begum ◽

ZA Begum ◽

MR Uddin ◽

AHMZ Haider ◽

RC Barman

Keyword(s):

Body Weight ◽

Acute Inflammation ◽

Methanol Extract ◽

Stem Bark ◽

Oedema Formation ◽

Medicinal Value ◽

Anti Inflammatory ◽

Inflammatory Effect

The plant Piper chaba Hunter (Piperaceae), a climbing glabrous shrub grows in plenty in southern Bangladesh. Popularly known as 'Choi' it is used as spices and believed to have medicinal value in a wide variety of disease conditions including arthritis, asthma, bronchitis and piles. In the present study, effect of methanol extract of Piper chaba stem bark on acute inflammation has been reported. The anti-inflammatory effect was studied in rats by injecting 0.1ml of 1% carrageenan suspension into the planter surface, where oedema of the rat's hind paw was used as an index of acute inflammation. Methanol extract of Piper chaba stem bark given orally 1 hour before injection at doses of 125 and 250 mg/kg body weight, produced significant (p< 0.05) anti inflammatory effect compared to control and the percentage of inhibition of oedema formation was 33% and 35% respectively, which however was less compared to aspirin (46%) and hydrocortisone (56%). The result suggest that in case of acute inflammation, Piper chaba stem bark possess mild to moderate anti inflammatory effect compared to that of aspirin and hydrocortisone.DOI: http://dx.doi.org/10.3329/fmcj.v7i1.10294Faridpur Med. Coll. J. 2012;7(1): 26-28

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Anti-inflammatory effect of the methanol extract from Anthocephalus cadamba stem bark in animal models

International Journal of Plant Biology ◽

10.4081/pb.2010.e6 ◽

2010 ◽

Vol 1 (1) ◽

pp. 6 ◽

Cited By ~ 6

Author(s):

Kodangala Subraya Chandrashekar ◽

Kodangala Subraya Prasanna ◽

Borthakur Abinash

Keyword(s):

Methanol Extract ◽

Stem Bark ◽

Cellular Migration ◽

Inflammatory Activity ◽

Traditional Use ◽

Aerial Parts ◽

Anti Inflammatory ◽

Design And Methods ◽

Anthocephalus Cadamba ◽

Inflammatory Effect

Background: Anthocephalus cadamba (Rebox)Miq. (Rubiaceae) is widely distributed throughout the greater part of India, especially at low levels in wet place. Traditionally the bark is used as tonic, febrifuge and to reduce the pain and inflammation. The anti-inflammatory effect of methanol extract obtained from Anthocephalus cadamba aerial parts, MEAC, were investigated in this study. Design and methods: The effects of MEAC on the acute and chronic phases of inflammation were studied in carrageenan, dextran and mediators (histamine and serotonin) induced paw edema and cotton pallet-induced granuloma, respectively. The anti-edema effect of MEAC was compared with 10 mg/kg of indomethacin orally. Results: The results suggested that MEAC possess potent anti-inflammatory activity. The acute inflammatory model showed that all the doses of MEAC effectively suppressed the edema produced by histamine, so it may be suggested that its anti-inflammatory activity is possibly backed by its antihistaminic activity. In chronic inflammatory model the effect may be due to the cellular migration to injured sites and accumulation of collagen and mucopolysaccharide. Conclusions: On the basis of these findings, it may be inferred that Anthocephalus cadamba is an anti-inflammatory agent and the results are in agreement with its traditional use.

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Effects of Methanol Extract of Piper chaba Stem Bark on Chronic Inflamation in Rats

Ibrahim Medical College Journal ◽

10.3329/imcj.v2i2.2934 ◽

1970 ◽

Vol 2 (2) ◽

pp. 37-39 ◽

Cited By ~ 2

Author(s):

Fouzia Begum ◽

Kamal Uddin ◽

Syeeda Sultana ◽

Abul Hasnat Ferdous ◽

Zinnat Ara Begum

Keyword(s):

Chronic Inflammation ◽

Acute Inflammation ◽

Methanol Extract ◽

Stem Bark ◽

Granuloma Formation ◽

Medicinal Value ◽

Cotton Pellet ◽

Anti Inflammatory ◽

Implantation Method ◽

Inflammatory Effect

Piper chaba Hunter (Piperaceae), a climbing glabrous shrub grows in plenty in southern Bangladesh. Popularly known as ‘Choi' it is used as spices and believed to have medicinal value in a wide variety of disease conditions including arthritis, asthma, bronchitis and piles. Earlier studies on methanol extract of Piper chaba stem bark have reported anti-inflammatory activities against acute inflammation. In the present study, effect of methanol extract of Piper chaba stem bark on chronic inflammation has been reported. The anti-inflammatory effect was studied in rats using cotton pellet implantation method, where granuloma formation was used as an index of chronic inflammation. Methanol extracts of Piper chaba stem bark given orally for 14 days daily at doses of 125 and 250 mg/kg body weight produced statistically significant (p < 0.05 and p < 0.01) anti-inflammatory effect compared to control. The percent inhibition of granuloma formation was 25% and 28% respectively, which however was less compared to aspirin (41%) and hydrocortisone (58%). The results suggest that in case of chronic inflammation, Piper chaba stem bark possess mild to moderate anti-inflammatory effect compared to that of aspirin and hydrocortisone. Ibrahim Med. Coll. J. 2008; 2(2): 37-39 Key Words: Rats, chronic inflammation, Piper chaba, aspirin, hydrocortisone, choi doi: 10.3329/imcj.v2i2.2934

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Analgesic and Anti-Inflammatory Activities of the Methanolic Stem Bark Extract ofNyctanthes arbor-tristisLinn.

BioMed Research International ◽

10.1155/2013/826295 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 6

Author(s):

Bibhuti Bhusan Kakoti ◽

Paresh Pradhan ◽

Sudarshana Borah ◽

Kabita Mahato ◽

Mritunjay Kumar

Keyword(s):

Body Weight ◽

Diclofenac Sodium ◽

Stem Bark ◽

Bark Extract ◽

Albino Rats ◽

Tail Flick ◽

Immersion Method ◽

Standard Drug ◽

Stem Bark Extract ◽

Anti Inflammatory

Stem bark ofNyctanthes arbor-tristisLinn. was extracted in methanol to evaluate their analgesic and anti-inflammatory activities. The analgesic activity was determined on Wistar albino rats by hot plate method, tail flick assay, and tail immersion method using Morphine sulphate as standard drug at a dose of 5 mg/kg of body weight and the results were expressed as mean increase in latency after drug administration ± SEM. The anti-inflammatory activity was assessed by Carrageenan-induced rat paw oedema using diclofenac sodium as standard drug at a dose of 100 mg/kg of body weight and expressed in terms of mean increase in paw volume ± SEM. Stem bark extract was given at a dose of 250 mg/kg and 500 mg/kg of body weight. Both standard drugs and extract were administered orally to the animals. Control received distilled water orally. Results showed thatNyctanthes arbor-tristisLinn. had potent analgesic and anti-inflammatory activities.

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Anti inflammatory effect of rhizome of Curcuma longa. Linn, in Albino rats by the method of Carrageenin induced paw oedema

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20180032 ◽

2018 ◽

Vol 7 (2) ◽

pp. 229 ◽

Cited By ~ 1

Author(s):

Jayan Pariyani Savaringal ◽

Lally M. S.

Keyword(s):

Curcuma Longa ◽

Ethanolic Extract ◽

Inflammatory Activity ◽

Albino Rats ◽

Standard Drug ◽

Medical College ◽

Inflammatory Conditions ◽

Anti Inflammatory ◽

Animal House ◽

Inflammatory Effect

Background: Curcuma longa or turmeric is a popular Ayurvedic herb, traditionally used for various inflammatory conditions including rheumatoid arthritis and spondylitis. Turmeric which containing phytochemical ingredient curcumin is used in India for centuries as a topical anti inflammatory agent. Many of the currently used anti inflammatory agents like NSAID’s and glucocorticoids have many undesirable adverse effects, especially when they are used for long period. In the present study, Curcuma longa, a plant belonging to the Zingiberaceae family was chosen for investigating its anti-inflammatory effects.Methods: The rhizomes of Curcuma longa were collected locally. The extract was prepared by soxlet extraction with 50% ethanol. Albino rats of Wistar strain (170-250grams) obtained from the animal house of medical college Thiruvananthapuram were used. Aspirin was purchased from sigma Labs, Mumbai. Anti-inflammatory effect of the extract was done in rats by the method of Carrageenin induced paw oedema.Results: The ethanolic extract of Curcuma longa inhibited the development of oedema at the end of 3 hours. The anti-inflammatory activity exhibited by the extract was dose dependent and statistically significant at dose levels of 1000-mg/kg and comparable to that of standard drug used Aspirin.Conclusions: The present study with extract of Curcuma longa revealed that it has significant anti-inflammatory activity.

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in vivo Anti-inflammatory Effect of Purified Aqueous and Methanol Extract of Trachyspermum ammi (Linn.) and Dolichos biflorus Linn. on Albino Rats

Asian Journal of Organic & Medicinal Chemistry ◽

10.14233/ajomc.2017.ajomc-p82 ◽

2017 ◽

Vol 2 (4) ◽

pp. 156-159 ◽

Cited By ~ 1

Author(s):

Shibani Basu ◽

Sekhar Mandal ◽

Tamoghna Maiti ◽

Jhuma Ganguly

Keyword(s):

Methanol Extract ◽

Albino Rats ◽

Dolichos Biflorus ◽

Trachyspermum Ammi ◽

Anti Inflammatory ◽

Inflammatory Effect

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Anti-inflammatory effect of Mitragyna speciosa crude methanol extract on the guinea pig ileum

Planta Medica ◽

10.1055/s-0029-1234736 ◽

2009 ◽

Vol 75 (09) ◽

Cited By ~ 1

Author(s):

WM Shaik Mossadeq ◽

K Syamimi ◽

MP Azyyati ◽

ZA Zakaria ◽

AK Arifah ◽

...

Keyword(s):

Guinea Pig ◽

Methanol Extract ◽

Guinea Pig Ileum ◽

Mitragyna Speciosa ◽

Anti Inflammatory ◽

Crude Methanol Extract ◽

Inflammatory Effect

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Evaluation of reversible antiandrogenic and antispermatogenic activities of Annona squamosa (Linn) stem bark methanol extract in male albino rats

Planta Medica ◽

10.1055/s-2006-949734 ◽

2006 ◽

Vol 72 (11) ◽

Author(s):

RS Gupta ◽

A Sharma ◽

H Rehwani

Keyword(s):

Methanol Extract ◽

Stem Bark ◽

Albino Rats ◽

Annona Squamosa

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Evaluation of anti-pyretic activity of Dracaena sanderiana by Brewer’s yeast method

International Journal of Novel Trends in Pharmaceutical Sciences ◽

10.26452/ijntps.v9i2.1200 ◽

2020 ◽

Vol 9 (2) ◽

pp. 32-34

Author(s):

Pavani C H

Keyword(s):

Herbal Remedies ◽

Standard Drug ◽

Chemical Substances ◽

Beneficial Effects ◽

Potential Value ◽

Plant Effects ◽

Therapeutic Properties ◽

Dracaena Sanderiana ◽

Anti Inflammatory ◽

Inflammatory Action

These medicinal plants are used to develop a therapy for the disease. To improve the science, investigate the scientific proof and activities validation, therefore the use of various herbal remedies for their pain-relieving and anti-inflammatory action in these current days. includes influence, anti-inflammatory, anti effect, analgesia, effects and some beneficial effects on the GI system. show the potential value of pain relief, cancer prevention and weight loss. According to these plant effects, consider that this present study was mainly based on to investigate and likely to reduce the fever caused by the outdoor and indoor. potential of is evidenced in leave studies. The medicinal plant produces a variety of chemical substances which shows significant therapeutic properties with the standard drug paracetamol.

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