Evaluation of 2 Hematology Analyzers in Body Fluid Mode versus Flow Cytometry Immunophenotyping of Mainly Neurosurgical Cerebrospinal Fluid Samples

2012 ◽  
Vol 73 (02) ◽  
pp. 093-098 ◽  
Author(s):  
B. Zur ◽  
L. Eichhorn ◽  
E. Albers ◽  
B. Stoffel-Wagner
Keyword(s):  
Flow Cytometry ◽  
Cerebrospinal Fluid ◽  
Body Fluid ◽  
Mode Versus ◽  
Hematology Analyzers

scholarly journals Study of body fluid samples using flow cytometry: Six years of experience at the Hospital Universitario San Ignacio - Pontificia Universidad Javeriana Bogota - Colombia

2017 ◽  
Vol 22 (2) ◽  
pp. 123
Author(s):  
Alba Campos ◽  
Laura Trujillo ◽  
Derly López ◽  
Lina Beltrán ◽  
Eliana Arias ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Cerebrospinal Fluid ◽  
Bronchoalveolar Lavage ◽  
Solid Tumors ◽  
Tumor Cells ◽  
Body Fluid ◽  
Hematological Malignancies ◽  
Pericardial Fluid ◽  
Malignant Diseases

Flow cytometry (FCM) was implemented in 2008 at the Pontificia Universidad Javeriana and later at the Hospital Universitario San Ignacio to examine special samples of patients with hematological malignancies and solid tumors other than bone marrow and peripheral blood for diagnosis and monitoring. This study describes the main findings of special sample evaluation over a six-year period. In all, 1070 samples of body fluids from patients with benign and malignant diseases were examined by FCM. These samples were stabilized with TransFixTM and stained with six-color immunophenotyping panels. Samples included cerebrospinal fluid, bronchoalveolar lavage, pleural fluid, pericardial fluid and ascite fluid from patients with acute and chronic leukemia, myelodysplastic syndromes, lymphomas, myeloma, autoimmune diseases, immunodeficiencies and solid tumors, among others. Flow cytometry provided important information for the classification and detection of minimal numbers of tumor cells in leukemia and lymphoma cases. This work represents the first national report describing FCM implementation in special samples for diagnosis and clinical monitoring of patients with malignant and benign pathologies.

scholarly journals EpCAM-based flow cytometry in cerebrospinal fluid greatly improves diagnostic accuracy of leptomeningeal metastases from epithelial tumors

2015 ◽  
Vol 18 (6) ◽  
pp. 855-862 ◽  
Author(s):  
Bojana Milojkovic Kerklaan ◽  
Dick Pluim ◽  
Mijke Bol ◽  
Ingrid Hofland ◽  
Johan Westerga ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cerebrospinal Fluid ◽  
Diagnostic Accuracy ◽  
Leptomeningeal Metastases ◽  
Epithelial Tumors

Cerebrospinal Fluid Cytology of Lyme Neuroborreliosis: A Report of 3 Cases with Literature Review

2015 ◽  
Vol 59 (4) ◽  
pp. 339-344 ◽  
Author(s):  
Juan Xing ◽  
Lisa Radkay ◽  
Sara E. Monaco ◽  
Christine G. Roth ◽  
Liron Pantanowitz
Keyword(s):  
Central Nervous System ◽  
Flow Cytometry ◽  
Cerebrospinal Fluid ◽  
Nervous System ◽  
Lyme Disease ◽  
B Cell ◽  
Plasma Cells ◽  
Lyme Neuroborreliosis ◽  
Chain Analysis ◽  
The Central Nervous System

Lyme disease can affect the central nervous system causing a B-cell-predominant lymphocytic pleocytosis. Since most reactions to infection in the cerebrospinal fluid (CSF) are typically T-cell predominant, a B-cell-predominant lymphocytosis raises concern for lymphoma. We present 3 Lyme neuroborreliosis cases in order to illustrate the challenging cytomorphological and immunophenotypic features of their CSF specimens. Three male patients who presented with central nervous system manifestations were diagnosed with Lyme disease. The clinical presentation, laboratory tests, CSF cytological examination and flow-cytometric studies were described for each case. CSF cytology showed lymphocytic pleocytosis with increased plasmacytoid cells and/or plasma cells. Flow cytometry showed the presence of polytypic B lymphocytes with evidence of plasmacytic differentiation in 2 cases. In all cases, Lyme disease was confirmed by the Lyme screening test and Western blotting. In such cases of Lyme neuroborreliosis, flow cytometry of CSF samples employing plasmacytic markers and cytoplasmic light-chain analysis is diagnostically helpful to exclude lymphoma.

Preliminary evaluation of UF-5000 Body Fluid Mode for automated cerebrospinal fluid cell counting

2017 ◽  
Vol 473 ◽  
pp. 133-138 ◽  
Author(s):  
Michela Seghezzi ◽  
Barbara Manenti ◽  
Giulia Previtali ◽  
Maria Grazia Alessio ◽  
Paola Dominoni ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Body Fluid ◽  
Cell Counting ◽  
Preliminary Evaluation ◽  
Fluid Cell

Cerebrospinal fluid analysis by 8-color flow cytometry in children with acute lymphoblastic leukemia

2019 ◽  
Vol 60 (11) ◽  
pp. 2825-2828 ◽  
Author(s):  
Maria Gabelli ◽  
Silvia Disarò ◽  
Pamela Scarparo ◽  
Samuela Francescato ◽  
Andrea Zangrando ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cerebrospinal Fluid ◽  
Acute Lymphoblastic Leukemia ◽  
Lymphoblastic Leukemia ◽  
Cerebrospinal Fluid Analysis ◽  
Color Flow ◽  
Fluid Analysis

scholarly journals Cerebrospinal Fluid Flow Cytometry: Utility in Central Nervous System Lymphoma Diagnosis

2020 ◽  
Vol 47 (3) ◽  
pp. 382-388
Author(s):  
Ka Loong Kelvin Au ◽  
Sarah Latonas ◽  
Afshin Shameli ◽  
Iwona Auer ◽  
Christopher Hahn
Keyword(s):  
Central Nervous System ◽  
Flow Cytometry ◽  
Cerebrospinal Fluid ◽  
Nervous System ◽  
Hematological Malignancy ◽  
Previous History ◽  
Primary Cns Lymphoma ◽  
Cns Lymphoma ◽  
Csf Flow ◽  
Neurologic Symptoms

ABSTRACT:Background:Flow cytometry of the cerebrospinal fluid (CSF) is used in isolation or as an adjunct to cytology to increase the sensitivity of detecting central nervous system (CNS) lymphoma. We aimed to evaluate the sensitivity of CSF flow cytometry as a diagnostic screening tool for primary CNS lymphoma in patients presenting with undifferentiated neurologic symptoms.Methods:We retrospectively reviewed all CSF samples received by the Calgary Laboratory Services Flow Cytometry Laboratory from 2012 to 2015. Clinical data, laboratory investigations, radiologic imaging studies, and pathological data were analyzed. Clinical review extended to 2 years post-CSF flow cytometric testing.Results:Only 43/763 (5.6%) samples of CSF flow cytometry in 28/573 (4.9%) patients were found to be positive for a hematological malignancy in patients with undifferentiated neurologic symptoms. The overall sensitivity of the test was 13.8% with 25 patients with negative CSF flow cytometry later having a positive biopsy for CNS lymphoma. CSF flow cytometry was negative in all cases when at the time of CSF examination the patient did not have a previous hematological malignancy or findings of abnormal enhancement on MRI (n = 249).Conclusion:CSF flow cytometry has low utility in screening for primary CNS lymphoma in the absence of a previous history of hematologic malignancy or findings of abnormal enhancement on MRI.

scholarly journals Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction

2019 ◽  
Vol 129 (5) ◽  
pp. e150-e154 ◽  
Author(s):  
Miles Berger ◽  
David M. Murdoch ◽  
Janet S. Staats ◽  
Cliburn Chan ◽  
Jake P. Thomas ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cerebrospinal Fluid ◽  
Cognitive Dysfunction ◽  
Postoperative Cognitive Dysfunction
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