Die Umweltprobenbank des Bundes: Die Bedeutung physiologischer Parameter für die Bewertung zeitlicher Trends der internen Schadstoffexposition durch Human-Biomonitoring

AbstractA simple and effective approach for HPLC-MS/MS based multi-mycotoxin analysis in human urine samples was developed by application of dried urine spots (DUS) as alternative on-site sampling strategy. The newly developed method enables the detection and quantitation of 14 relevant mycotoxins and mycotoxin metabolites, including citrinin (CIT), dihydrocitrinone (DH-CIT), deoxynivalenol (DON), fumonisin B1 (FB1), T-2 Toxin (T-2), HT-2 Toxin (HT-2), ochratoxin A (OTA), 2′R-ochratoxin A (2′R-OTA), ochratoxin α (OTα), tenuazonic acid and allo-tenuazonic acid (TeA + allo-TeA), zearalenone (ZEN), zearalanone (ZAN), α-zearalenol (α-ZEL), and β-zearalenol (β-ZEL). Besides the spotting procedure, sample preparation includes enzymatic cleavage of glucuronic acid conjugates and stable isotope dilution analysis. Method validation revealed low limits of detection in the range of pg/mL urine and excellent apparent recovery rates for most analytes. Stability investigation of DUS displayed no or only slight decrease of the analyte concentration over a period of 28 days at room temperature. The new method was applied to the analysis of a set of urine samples (n = 91) from a Swedish cohort. The four analytes, DH-CIT, DON, OTA, and TeA + allo-TeA, could be detected and quantified in amounts ranging from 0.06 to 0.97 ng/mL, 3.03 to 136 ng/mL, 0.013 to 0.434 ng/mL and from 0.36 to 47 ng/mL in 38.5%, 70.3%, 68.1%, and 94.5% of the samples, respectively. Additional analysis of these urine samples with an established dilute and shoot (DaS) approach displayed a high consistency of the results obtained with both methods. However, due to higher sensitivity, a larger number of positive samples were observed using the DUS method consequently providing a suitable approach for human biomonitoring of mycotoxin exposure.

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Citrinin Dietary Exposure Assessment Approach through Human Biomonitoring High-Resolution Mass Spectrometry-Based Data

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.1c01776 ◽

2021 ◽

Author(s):

Alfonso Narváez ◽

Luana Izzo ◽

Yelko Rodríguez-Carrasco ◽

Alberto Ritieni

Keyword(s):

Mass Spectrometry ◽

High Resolution ◽

Exposure Assessment ◽

High Resolution Mass Spectrometry ◽

Dietary Exposure ◽

Human Biomonitoring ◽

High Resolution Mass ◽

Assessment Approach ◽

Resolution Mass

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Exposure assessment of Spanish lactating mothers to acrylamide via human biomonitoring

Environmental Research ◽

10.1016/j.envres.2021.111832 ◽

2021 ◽

pp. 111832

Author(s):

Sandra F. Fernández ◽

Olga Pardo ◽

Clara Coscollà ◽

Vicent Yusà

Keyword(s):

Exposure Assessment ◽

Human Biomonitoring ◽

Lactating Mothers

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Pentachlorophenol and nine other chlorophenols in urine of children and adolescents in Germany – Human biomonitoring results of the German Environmental Survey 2014–2017 (GerES V)

Environmental Research ◽

10.1016/j.envres.2021.110958 ◽

2021 ◽

Vol 196 ◽

pp. 110958

Author(s):

Maria I.H. Schmied-Tobies ◽

Aline Murawski ◽

Lukas Schmidt ◽

Enrico Rucic ◽

Gerda Schwedler ◽

...

Keyword(s):

Children And Adolescents ◽

Human Biomonitoring ◽

Environmental Survey

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Chemical Exposure: European Citizens’ Perspectives, Trust, and Concerns on Human Biomonitoring Initiatives, Information Needs, and Scientific Results

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18041532 ◽

2021 ◽

Vol 18 (4) ◽

pp. 1532

Author(s):

Maria Uhl ◽

Ricardo R. Santos ◽

Joana Costa ◽

Osvaldo Santos ◽

Ana Virgolino ◽

...

Keyword(s):

Decision Making ◽

Policy Development ◽

Information Needs ◽

Policy Decision ◽

Human Biomonitoring ◽

Chemical Exposure ◽

The European Union ◽

Cross Sectional ◽

The United Kingdom ◽

Scientific Results

Over the last few decades, citizen awareness and perception of chemical products has been a topic of interest, particularly concerning national and international policy decision makers, expert/scientific platforms, and the European Union itself. To date, few qualitative studies on human biomonitoring have analysed communication materials, made recommendations in terms of biomonitoring surveillance, or asked for feedback in terms of specific biomonitoring methods. This paper provides in-depth insight on citizens’ perceptions of knowledge of biomonitoring, impact of chemical exposure on daily life, and claims on how results of research should be used. Four semi-structured focus groups were held in Austria, Portugal, Ireland, and the United Kingdom (UK). The cross-sectional observational qualitative design of this study allows for better understanding of public concern regarding chemicals, application, and use of human biomonitoring. The main findings of this study include citizens’ clear articulation on pathways of exposure, the demand on stakeholders for transparent decision-making, and sensitivity in communication of results to the public. Validated and trustful communication is perceived as key to empowering citizens to take action. The results can be used to facilitate decision-making and policy development, and feeds into the awareness needs of similar and future projects in human biomonitoring. Furthermore, it also brings to light ideas and concepts of citizens’ in shaping collaborative knowledge between citizens’, experts, scientists, and policy makers on equal terms.

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Metabolic syndrome and endocrine disrupting chemicals: exposure and health effects

European Journal of Public Health ◽

10.1093/eurpub/ckaa166.086 ◽

2020 ◽

Vol 30 (Supplement_5) ◽

Author(s):

E Haverinen ◽

R Lange ◽

H Tolonen

Keyword(s):

Metabolic Syndrome ◽

Bisphenol A ◽

Health Effects ◽

Endocrine Disrupting Chemicals ◽

Positive Association ◽

Human Biomonitoring ◽

European Population ◽

Priority Substances ◽

Metabolic Disturbances ◽

Endocrine Disrupting

Abstract Increasing prevalence of metabolic syndrome (MetS) is causing significant health burden among the European population. Current knowledge supports the notion that endocrine disrupting chemicals (EDCs) interfere with human metabolism and hormonal balance, contributing to the conventionally recognized life-style related risk factors for MetS. In relation to the Human biomonitoring initiative (HBM4EU) five priority substances (Bisphenol A, Per- and polyfluoroalkyl substances (PFASs), Phthalates, Cadmium and Arsenic) and their association with adverse metabolic health effects were examined. A methodological framework for scoping reviews was followed to increase consistency and transparency throughout the process. A literature review was conducted to identify epidemiological studies focusing on the association between MetS or its individual components and the five HBM4EU priority substances. Human biomonitoring studies have been able to present evidence supporting EDC exposure and development of individual MetS components; however the strength of the association varies between the components and EDCs. Most of the identified literature examined Bisphenol A and Phthalate exposure, usually targeting obesity, anthropometrics or glucose metabolism. Evidence suggests a positive association between Bisphenol A and Phthalate exposure and obesity-related components. The substance group of PFASs indicated weakest association, as the results were inconsistent and were suggestive only for a positive association with development of dyslipidaemia. Current evidence on metabolic disturbances and EDCs are inconclusive and fragmented, hence establishing harmonized and standardized human biomonitoring procedures among the European population are needed. Rigorous and ongoing human biomonitoring in combination with health monitoring could provide comprehensive information on EDC exposure and association of metabolic disturbances. Key messages EDC exposure is ubiquitous within European population, hence more human biomonitoring in combination with health surveys is needed to strengthen knowledge on human’s metabolic health. MetS is an increasing global health concern, which requires novel approaches to tackle the challenge.

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