The transcription factor activating protein 2 beta (TFAP2B) mediates neuronal differentiation in neuroblastoma cells

In all vertebrate species, the homeobox gene goosecoid serves as a marker of the Spemann organizer tissue. One function of the organizer is the induction of neural tissue. To investigate the role of goosecoid in neuronal differentiation of mammalian cells, we have introduced goosecoid into PC12 cells. Expression of goosecoid resulted in reduced cell proliferation and enhanced neurite outgrowth in response to NGF. Expression of goosecoid led to a decrease in the percentage of S-phase cells and to upregulation of the expression of the neuron-specific markers MAP-1b and neurofilament-L. Analysis of goosecoid mutants revealed that these effects were independent of either DNA binding or homodimerization of Goosecoid. Coexpression of the N-terminal portion of the ets transcription factor PU.1, a protein that can bind to Goosecoid, repressed neurite outgrowth and rescued the proliferation of PC12 cultures. In contrast, expression of the bHLH transcription factor HES-1 repressed goosecoid-mediated neurite outgrowth without changing the proportion of S-phase cells. These results suggest that goosecoid is involved in neuronal differentiation in two ways, by slowing the cell cycle and stimulating neurite outgrowth, and that these two events are separately regulated.

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Transcriptomic profile of Pea3 family members reveal regulatory codes for axon outgrowth and neuronal connection specificity

Scientific Reports ◽

10.1038/s41598-020-75089-3 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Başak Kandemir ◽

Gizem Gulfidan ◽

Kazim Yalcin Arga ◽

Bayram Yilmaz ◽

Isil Aksan Kurnaz

Keyword(s):

Transcription Factor ◽

Target Genes ◽

Neural Circuit ◽

Molecular Model ◽

Expression Profiles ◽

Neuroblastoma Cells ◽

Branching Morphogenesis ◽

Subfamily Member ◽

Circuit Formation ◽

Cell Cell

Abstract PEA3 transcription factor subfamily is present in a variety of tissues with branching morphogenesis, and play a particularly significant role in neural circuit formation and specificity. Many target genes in axon guidance and cell–cell adhesion pathways have been identified for Pea3 transcription factor (but not for Erm or Er81); however it was not so far clear whether all Pea3 subfamily members regulate same target genes, or whether there are unique targets for each subfamily member that help explain the exclusivity and specificity of these proteins in neuronal circuit formation. In this study, using transcriptomics and qPCR analyses in SH-SY5Y neuroblastoma cells, hypothalamic and hippocampal cell line, we have identified cell type-specific and subfamily member-specific targets for PEA3 transcription factor subfamily. While Pea3 upregulates transcription of Sema3D and represses Sema5B, for example, Erm and Er81 upregulate Sema5A and Er81 regulates Unc5C and Sema4G while repressing EFNB3 in SH-SY5Y neuroblastoma cells. We furthermore present a molecular model of how unique sites within the ETS domain of each family member can help recognize specific target motifs. Such cell-context and member-specific combinatorial expression profiles help identify cell–cell and cell-extracellular matrix communication networks and how they establish specific connections.

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Neuronal ‘differentiation’ of murine neuroblastoma cells induced by neocarzinostatin: neural cell adhesion molecules

Brain Research ◽

10.1016/0006-8993(93)90462-v ◽

1993 ◽

Vol 613 (1) ◽

pp. 123-131 ◽

Cited By ~ 3

Author(s):

Jeffrey A. Lowengrub ◽

Nina Felice Schor

Keyword(s):

Cell Adhesion ◽

Neuronal Differentiation ◽

Adhesion Molecules ◽

Cell Adhesion Molecules ◽

Neuroblastoma Cells ◽

Neural Cell ◽

Neural Cell Adhesion Molecules ◽

Murine Neuroblastoma ◽

Neural Cell Adhesion

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Radiation-Induced Calreticulin Expression Leads to MYCN Suppression and Neuronal Differentiation in Neuroblastoma Cells

International Journal of Radiation Oncology*Biology*Physics ◽

10.1016/j.ijrobp.2018.07.690 ◽

2018 ◽

Vol 102 (3) ◽

pp. e190-e191

Author(s):

J.H. Hong ◽

A.C.L. Lee ◽

Y.Y. Shih

Keyword(s):

Neuronal Differentiation ◽

Neuroblastoma Cells ◽

Radiation Induced

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Neoglycosylated Collagen: Effect on Neuroblastoma F-11 Cell Lines

Molecules ◽

10.3390/molecules25194361 ◽

2020 ◽

Vol 25 (19) ◽

pp. 4361

Author(s):

Antonella Sgambato ◽

Valentina Pastori ◽

Laura Russo ◽

Simone Vesentini ◽

Marzia Lecchi ◽

...

Keyword(s):

Cell Proliferation ◽

Nervous System ◽

Regenerative Medicine ◽

Neuronal Differentiation ◽

Cell Lines ◽

Neuroblastoma Cells ◽

Biological Significance ◽

Great Promise ◽

Cell Behavior ◽

Carbohydrate Epitopes

The regeneration of the nervous system is a challenging task. Currently, regenerative medicine approaches that exploit nature-inspired cues are being studied and hold great promise. The possibility to use protein-based matrices functionalized with small oligo- and monosaccharides is of interest since these can be finely tuned to better mimic the native environment. Collagen has been selected as a promising material that has the potential to be further tailored to incorporate carbohydrates in order to drive cell behavior towards neuroregeneration. Indeed, the grafting of carbohydrates to collagen 2D matrices is proved to enhance its biological significance. In the present study, collagen 2D matrices were grafted with different carbohydrate epitopes, and their potential to drive F-11 neuroblastoma cells towards neuronal differentiation was evaluated. Collagen functionalized with α-glucosides was able to differentiate neuroblastoma cells into functional neurons, while sialyl α-(2→6)-galactosides stimulated cell proliferation.

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