Regenerative Therapie von Sehnen- und Banderkrankungen bei Pferden

2011 ◽  
Vol 39 (06) ◽  
pp. 373-383 ◽  
Author(s):  
P. Stadler ◽  
F. Geburek
Keyword(s):  
Urinary Bladder ◽  
Urinary Bladder Matrix ◽  
Regenerative Therapie ◽  
Thrombozytenreiches Plasma

ZusammenfassungHerkömmliche Therapieansätze führen bei Pferden zu einer unbefriedigenden Sehnenheilung mit einer relativ hohen Rezidivrate. Deshalb werden seit einigen Jahren unter anderem in vitro und an Labortieren so genannte regenerative Therapieformen wissenschaftlich untersucht. Darunter wird der Einsatz von Substanzen verstanden, die idealerweise die Bildung eines Ersatzgewebes bewirken, das vergleichbare funktionelle Eigenschaften hat wie das Ursprungsgewebe und sich somit von minderwertigerem Narbengewebe abhebt. Derzeit ist eine Fülle verschiedener Substrate entweder kommerziell verfügbar oder mithilfe von „Kits“ unter Praxisbedingungen herstellbar. Es werden die wesentlichen Erkenntnisse zur Herstellung und zum regenerativen Potenzial von kernhaltigen Zellen bzw. Stammzellen aus Knochenmark und Fettgewebe sowie der Blutprodukte PRP (thrombozytenreiches Plasma), ACP (autologes konditioniertes Plasma), ACS (autologes konditioniertes Serum) und der Gerüstsubstanz UBM (urinary bladder matrix) vorgestellt. Zuletzt wird das Potenzial einzelner Wachstumsfaktoren und der Gentherapie beleuchtet. Bisher wird angenommen, dass die Regeneration von Sehnengewebe durch ein komplexes Zusammenspiel aus Gerüstsubstanzen, Wachstumsfaktoren und Zellen begünstigt wird. Es sind bislang nur wenige Untersuchungen verfügbar, die einen Vergleich zwischen den genannten Substanzen erlauben. Untersuchungen zur Wirkung der Substrate an lebenden Pferden werden an anderer Stelle vorgestellt.

Evaluation of the bioactivity about anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold for pelvic reconstruction

2018 ◽  
Vol 33 (6) ◽  
pp. 808-818 ◽  
Author(s):  
Jiankui Li ◽  
Xi Chen ◽  
Kaijian Ling ◽  
Zhiqing Liang ◽  
Huicheng Xu
Keyword(s):  
Growth Factor ◽  
Urinary Bladder ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Pelvic Organ ◽  
Fibroblast Growth ◽  
Pelvic Reconstruction ◽  
Urinary Bladder Matrix

Introduction and hypothesis: Pelvic support structure injury is the major cause of pelvic organ prolapse. At present, polypropylene-based filler material has been suggested as a common method to treat pelvic organ prolapse. However, it cannot functionally rehabilitate the pelvic support structure. In addition to its poor long-term efficiency, the urinary bladder matrix was the most suitable biological scaffold material for pelvic floor repair. Here, we hypothesize that anti-sca-1 monoclonal antibody and basic fibroblast growth factor were cross-linked to urinary bladder matrix to construct a two-factor bioscaffold for pelvic reconstruction. Methods Through a bispecific cross-linking reagent, sulfosuccinimidyl 4-[N-maleimidomethyl] cyclohexane-1-carboxylate (sulfo-smcc) immobilized anti-sca-1 and basic fibroblast growth factor to urinary bladder matrix. Then scanning electron microscope and plate reader were used to detect whether the anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold was built successfully. After that, the capacity of enriching sca-1 positive cells was measured both in vitro and in vivo. In addition, we evaluated the differentiation capacity and biocompatibility of the scaffold. Finally, western blotting was used to detect the level of fibulin-5 protein. Results The scanning electron microscope and plate reader revealed that the double-factor biological scaffold was built successfully. The scaffold could significantly enrich a large number of sca-1 positive cells both in vitro and in vivo, and obviously accelerate cells and differentiate functional tissue with good biocompatibility. Moreover, the western blotting showed that the scaffold could improve the expression of fibulin-5 protein. Conclusion The anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold revealed good biological properties and might serve as an ideal scaffold for pelvic reconstruction.

scholarly journals Urinary bladder matrix does not improve tenogenesis in an in vitro equine model

2019 ◽  
Author(s):  
Sarah M Khatibzadeh ◽  
Bruno C Menarim ◽  
Anne EC Nichols ◽  
Stephen R Werre ◽  
Linda A Dahlgren
Keyword(s):  
Urinary Bladder ◽  
Urinary Bladder Matrix

scholarly journals Urinary Bladder Matrix Does Not Improve Tenogenesis in an In Vitro Equine Model

2019 ◽  
Vol 37 (8) ◽  
pp. 1848-1859
Author(s):  
Sarah M. Khatibzadeh ◽  
Bruno C. Menarim ◽  
Anne E. C. Nichols ◽  
Stephen R. Werre ◽  
Linda A. Dahlgren
Keyword(s):  
Urinary Bladder ◽  
Urinary Bladder Matrix

scholarly journals Characterization of Porcine Urinary Bladder Matrix Hydrogels from Sodium Dodecyl Sulfate Decellularization Method

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3007
Author(s):  
Chen-Yu Kao ◽  
Huynh-Quang-Dieu Nguyen ◽  
Yu-Chuan Weng
Keyword(s):  
Urinary Bladder ◽  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Potential Candidate ◽  
Gelation Kinetics ◽  
Urinary Bladder Matrix ◽  
Dodecyl Sulfate ◽  
Almost All

Urinary bladder matrix (UBM) is one of the most studied extracellular matrixes (ECM) in the tissue engineering field. Although almost all of the UBM hydrogels were prepared by using peracetic acid (PAA), recent studies indicated that PAA was not a trustworthy way to decellularize UBM. A stronger detergent, such as sodium dodecyl sulfate (SDS), may help tackle this issue; however, its effects on the hydrogels’ characteristics remain unknown. Therefore, the objective of this study was to develop a more reliable protocol to decellularize UBM, using SDS, and to compare the characteristics of hydrogels obtained from this method to the widely employed technique, using PAA. The results indicated that SDS was superior to PAA in decellularization efficacy. Different decellularization methods led to dissimilar gelation kinetics; however, the methods did not affect other hydrogel characteristics in terms of biochemical composition, surface morphology and rheological properties. The SDS-treated hydrogels possessed excellent cytocompatibility in vitro. These results showed that the SDS decellularization method could offer a more stable and safer way to obtain acellular UBM, due to reducing immunogenicity. The hydrogels prepared from this technique had comparable characteristics as those from PAA and could be a potential candidate as a scaffold for tissue remodeling.

scholarly journals Evaluating the Effect of Tissue Selection on the Characteristics of Extracellular Matrix Hydrogels from Decellularized Porcine Bladders

2021 ◽  
Vol 11 (13) ◽  
pp. 5820
Author(s):  
Chen-Yu Kao ◽  
Huynh-Quang-Dieu Nguyen ◽  
Yu-Chuan Weng ◽  
Yu-Han Hung ◽  
Chun-Min Lo
Keyword(s):  
Extracellular Matrix ◽  
Urinary Bladder ◽  
Mass Production ◽  
Biochemical Composition ◽  
In Vitro Studies ◽  
Biochemical Effects ◽  
Gelation Kinetics ◽  
Urinary Bladder Matrix ◽  
Potential Use

Porcine urinary bladder is one of the most used organs to fabricate extracellular matrix (ECM) hydrogel. Although there are two different ECM types inside a bladder, i.e., urinary bladder matrix (UBM) and a subtype ECM (sECM), most studies have only employed UBM for hydrogel fabrication, and overlooked the potential use of sECM. In another aspect, the delamination of UBM from bladders is a time-consuming process; consequently, the use of the whole bladder (WB) will likely increase production yield. Therefore, the objective of this study was to fabricate hydrogels from sECM and WB and compare them to UBM. The results indicated that different layers of the bladder shared almost the same biochemical composition. In terms of gelation kinetics, rheology and morphology, although hydrogels from UBM and sECM exhibited some discrepancies, those from UBM and WB interestingly possessed almost the same characteristics. In in vitro studies, all the hydrogels possessed nearly the same biochemical effects towards L929 viability and C2C12 differentiation. These results could preliminarily indicate that the use of sECM should no longer be ignored, and WB could be a promising substitution for UBM hydrogels, eliminating the need for time-consuming delamination processes, as well as increasing the possibility of mass production.

Effect of Acellular Bovine Pericardium and Urinary Bladder Submucosa Matrixes in Reconstruction of Ventro-Lateral Hernias in Bucks; Molecular Evaluation

2019 ◽  
Vol 43 (1) ◽  
pp. 67-74
Author(s):  
Areeg K. M. Al-ebadi
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Treatment Group ◽  
Urinary Bladder ◽  
Bovine Pericardium ◽  
Vascular Endothelial ◽  
Fibroblast Growth ◽  
Urinary Bladder Matrix ◽  
Endothelial Growth Factor ◽  
Molecular Evaluation

The present study aimed to estimate the efficiency of both a cellular bovine pericardium and bovine urinary bladder matrix sheets in the reconstruction of large ventro-lateral hernias in Iraqi bucks by using of molecular evaluation depending on real time-polymerase chain reaction technique to investigate the level of basic-fibroblast growth factor  and vascular endothelial growth factor  genes during the healing process and reconstruction of the abdominal defects. Under sedation and local anesthesia, (6cm X 8cm size) of ventro-lateral hernias were induced in 24 of Iraqi bucks. The animals were divided randomly into two main equal groups. In bovine pericardium-treatment group, the hernias were treated with onlay implantation of bovine pericardium. While, the hernias in UBM-treatment group were treated with onlay implantation of urinary bladder matrix, 30 days post-inducing of hernias. The molecular evaluation along the period of following-up recorded a significant up-regulation of the level of basic-fibroblast growth factor gene specific for presence of fibroblasts, myofibroblasts and collagen deposition in urinary bladder matrix -treatment group in comparison to bovine pericardium -treatment group with significant difference even at the end of the study. While, a significant up regulation of the levels of angiogenesis classic gene vascular endothelial growth factor  were recorded in the bucks of bovine pericardium -treatment group compared to urinary bladder matrix -treatment group. In conclusion; molecular detection of the level of growth factors in target tissue can be used as an important criterion.

OSMOTIC INHIBITION OF THE NATRIFERIC RESPONSE OF THE TOAD URINARY BLADDER TO VASOPRESSIN

1975 ◽  
Vol 67 (1) ◽  
pp. 119-125
Author(s):  
P. J. BENTLEY
Keyword(s):  
Urinary Bladder ◽  
Water Movement ◽  
Short Circuit ◽  
Electrical Potential ◽  
Short Circuit Current ◽  
Toad Bladder ◽  
Toad Urinary Bladder ◽  
Electrical Potential Difference ◽  
Osmotic Gradient

SUMMARY The electrical potential difference and short-circuit current (scc, reflecting active transmural sodium transport) across the toad urinary bladder in vitro was unaffected by the presence of hypo-osmotic solutions bathing the mucosal (urinary) surface, providing that the transmural flow of water was small. Vasopressin increased the scc across the toad bladder (the natriferic response), but this stimulation was considerably reduced in the presence of a hypo-osmotic solution on the mucosal side, conditions under which water transfer across the membrane was also increased. This inhibition of the natriferic response did not depend on the direction of the water movement, for if the osmotic gradient was the opposite way to that which normally occurs, the response to vasopressin was still reduced. The natriferic response to cyclic AMP was also inhibited in the presence of an osmotic gradient. Aldosterone increased the scc and Na+ transport across the toad bladder but this response was not changed when an osmotic gradient was present. The physiological implications of these observations and the possible mechanisms involved are discussed.

scholarly journals Cystitis increases colorectal afferent sensitivity in the mouse

2009 ◽  
Vol 297 (6) ◽  
pp. G1250-G1258 ◽  
Author(s):  
Pablo Rodolfo Brumovsky ◽  
Bin Feng ◽  
Linjing Xu ◽  
Carly Jane McCarthy ◽  
G. F. Gebhart
Keyword(s):  
Urinary Bladder ◽  
Treated Group ◽  
Mechanical Sensitivity ◽  
Pelvic Nerve ◽  
Treatment Groups ◽  
Colon Inflammation ◽  
Afferent Fibers ◽  
Peripheral Mechanism ◽  
Inflammatory Soup

Studies in humans and rodents suggest that colon inflammation promotes urinary bladder hypersensitivity and, conversely, that cystitis contributes to colon hypersensitivity, events referred to as cross-organ sensitization. To investigate a potential peripheral mechanism, we examined whether cystitis alters the sensitivity of pelvic nerve colorectal afferents. Male C57BL/6 mice were treated with cyclophosphamide (CYP) or saline, and the mechanosensitive properties of single afferent fibers innervating the colorectum were studied with an in vitro preparation. In addition, mechanosensitive receptive endings were exposed to an inflammatory soup (IS) to study sensitization. Urinary bladder mechanosensitive afferents were also tested. We found that baseline responses of stretch-sensitive colorectal afferents did not differ between treatment groups. Whereas IS excited a proportion of colorectal afferents CYP treatment did not alter the magnitude of this response. However, the number of stretch-sensitive fibers excited by IS was increased relative to saline-treated mice. Responses to IS were not altered by CYP treatment, but the proportion of IS-responsive fibers was increased relative to saline-treated mice. In bladder, IS application increased responses of muscular afferents to stretch, although no differences were detected between saline- and CYP-treated mice. In contrast, their chemosensitivity to IS was decreased in the CYP-treated group. Histological examination revealed no changes in colorectum and modest edema and infiltration in the urinary bladder of CYP-treated mice. In conclusion, CYP treatment increased mechanical sensitivity of colorectal muscular afferents and increased the proportion of chemosensitive colorectal afferents. These data support a peripheral contribution to cross-organ sensitization of pelvic organs.

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