On the Role of the Stable Plasminogen Activator of the Venous Wall in Occlusion Induced Fibrinolytic State

1969 ◽  
Vol 22 (03) ◽  
pp. 544-551 ◽  
Author(s):  
R Constantini ◽  
F Spöttl ◽  
F Holzknecht ◽  
H Braunsteiner
Keyword(s):  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Venous Blood ◽  
Venous Occlusion ◽  
Histochemical Method ◽  
Before And After ◽  
Venous Wall

SummaryThe fibrinolytic activity respectively the PA activity of normal veins before and after venous occlusion is measured by the histochemical method of Todd. The results show marked decreases of PA of the adventitia and an increase of PA of the endothelial type, after venous occlusion. This suggests a transfer of PA through the venous wall into the intravascular lumen, and may be the cause of increased fibrinolytic activity in venous blood after occlusion. Contrary assumptions are discussed.

Enhancement of Fibrinolysis by Venous Occlusion Results of Long-Term Thrombelastography and Activator Determination

1969 ◽  
Vol 21 (02) ◽  
pp. 304-310 ◽  
Author(s):  
F Holzknecht ◽  
F Spöttl ◽  
H Braunsteiner
Keyword(s):  
Strong Correlation ◽  
Fibrinolytic Activity ◽  
Considerable Increase ◽  
Venous Blood ◽  
Venous Occlusion ◽  
Venous Congestion ◽  
Low Speed ◽  
Before And After

SummaryGlobal fibrinolysis and activator activity were investigated in venous blood before and after occlusion. Global fibrinolysis was determinated by thrombelastography with a low speed of the paper drive (longterm thrombelastography). The fibrinolysis on the thrombelastographic curves were evaluated by the slant (a°) of the inflectional tangent at the thrombelastographic branch.A considerable increase of global fibrinolytic activity and also of the activator activity after venous congestion was found. There was a slight correlation between the global fibrinolysis and the activator before occlusion and a strong correlation after congestion. There was, however, no accordance between the regression lines of the two correlations. Other factors of activation of fibrinolysis may be involved.

FIBRINOLYTIC ACTIVITY OF ARMS AND LE6S IN PERIPHERAL VENOUS HYPERTENSION IN MAN

1987 ◽  
Author(s):  
M R Carriero ◽  
F Annoni ◽  
L Mussoni ◽  
C Cerletti ◽  
G de Gaetano
Keyword(s):  
Hydrostatic Pressure ◽  
Fibrinolytic Activity ◽  
Diastolic Pressure ◽  
Venous Blood ◽  
Normal Subjects ◽  
Venous Occlusion ◽  
Venous Hypertension ◽  
Average Increase ◽  
Before And After

Spontaneous fibrinolytic activity of venous specimens Is greater In the arms than in the legs of normal subjects. This difference might be caused by the different hydrostatic pressure In arms and legs. We tested, on standard fibrin plates, the fibrinolytic activity of euglobulins prepared from venous blood obtained from arms and legs of normal subjects and patients with chronic peripheral hypertension. Normal subjects (26-38 yrs old, n=5) were tested both before and after 10 min venous occlusion (V0) of an arm and after 10 min occlusion of a leg. V0 was obtained by applying the cuff of a sphlgmomanometer at a pressure value Intermediate between systolic and diastolic pressure. Patients (39-64 yrs old, n=7) were tested both before and after V0 of the arm and after 10 min orthostatic posture (mean 100 mmHg). For each Individual the fibrinolytic activity In the arm before V0 was considered as basal value of both the arm and the leg. In normal subjects fibrinolytic activity Induced by V0 was greater In the arm than In the leg (262.9°74.9 versus 165.5°52.9 mm2). The average Increase of fibrinolytic activity after V0 was 3.4 (arms) and 2.1 (legs). In patients with peripheral venous hypertension fibrinolytic activity was 298.3°46.7 mm2 In the arm and 131.1 °19.2 mm2 In the leg. The average Increase Induced by VO In the arm was 3.5 while the activity of the legs after orthostatic pressure was 1.6. In conclusion patients with peripheral venous hypertension did not show any reduced fibrinolytic response after VO of the arms. Fibrinolytic activity in patients" legs after orthostatic pressure was also similar to that In the legs of volunteers after venous occlusion.

Fibrinolytic Activity in Chinese Patients with Diabetes or Hyperlipidemia in Comparison with Healthy Controls

1991 ◽  
Vol 65 (01) ◽  
pp. 003-006 ◽  
Author(s):  
Chao Hung Ho ◽  
Tjin Shing Jap
Keyword(s):  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Plasminogen Activator Inhibitor ◽  
Venous Occlusion ◽  
Chinese Patients ◽  
Tissue Type ◽  
Diabetic Patients ◽  
Healthy Controls ◽  
Before And After ◽  
Patients With Diabetes

SummaryPlasma tissue-type plasminogen activator (tPA), plasminogen activator inhibitor (PAI) and euglobulin lysis time (ELT) were determined before and after the venous occlusion test (VOT) in 3 groups of patients with mean age about 60 years: 29 diabetic patients (D group), 8 hyperlipidemic patients (H group) and 19 healthy controls (C group). In the D and H groups, the mean of morning tPA was significantly higher than that of the C grogp, but the means of PAI were not significantly different among the 3 groups. ELI was significantly shortened and tPA was markedly increased after the VOT in all 3 groups whereas PAI had not significantly changed. In conclusion, high tPA activity and good fibrinolytic response without significant change of PAI .activity were found in the diabetic and hyperlipidemic patients, and no definite impairment of the fibrinolytic activity could be found in the Chinese patients with diabetes and hyperlipidemia. This might be one of the reasons why the Chinese has low incidence of thromboembolic diseases.

Fibrinolysis in Normal Subjects - Comparison Between Plasminogen Activator Inhibitor and Other Components of the Fibrinolytic System

1988 ◽  
Vol 59 (02) ◽  
pp. 299-303 ◽  
Author(s):  
Grazia Nicoloso ◽  
Jacques Hauert ◽  
Egbert K O Kruithof ◽  
Guy Van Melle ◽  
Fedor Bachmann
Keyword(s):  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Plasminogen Activator Inhibitor ◽  
Venous Occlusion ◽  
Venous Stasis ◽  
Plasminogen Activator Inhibitor 1 ◽  
Plate Assay ◽  
Fibrin Plate ◽  
Activator Inhibitor ◽  
Pai 1

SummaryWe analyzed fibrinolytic parameters in 20 healthy men and 20 healthy women, aged from 25 to 59, before and after 10 and 20 min venous occlusion. The 10 min post-occlusion fibrinolytic activity measured directly in diluted unfractionated plasma by a highly sensitive 125I-fibrin plate assay correlated well with the activity of euglobulins determined by the classical fibrin plate assay (r = 0.729), but pre-stasis activities determined with these two methods did not correlate (r = 0.084). The enhancement of fibrinolytic activity after venous occlusion was mainly due to an increase of t-PA in the occluded vessels (4-fold increase t-PA antigen after 10 min and 8-fold after 20 min venous occlusion). Plasminogen activator inhibitor (PAI) activity and plasminogen activator inhibitor 1 (PAI-1)1 antigen levels at rest showed considerable dispersion ranging from 1.9 to 12.4 U/ml, respectively 6.9 to 77 ng/ml. A significant increase of PAI-1 antigen levels was observed after 10 and 20 min venous occlusion. At rest no correlation was found between PAI activity or PAI-1 antigen levels and the fibrinolytic activity measured by 125I-FPA. However, a high level of PAI-1 at rest was associated with a high prestasis antigen level of t-PA and a low fibrinolytic response after 10 min of venous stasis. Since the fibrinolytic response inversely correlated with PAI activity at rest, we conclude that its degree depends mainly on the presence of free PAI.

PHYSICAL ACTIVITY RELEASES TISSUE PLASMINOGEN ACTIVATOR FROM EXERCISING PARTS OF BODY

1987 ◽  
Author(s):  
I Keber ◽  
K Potisk ◽  
D Keber ◽  
M Stegnar ◽  
N Vene
Keyword(s):  
Physical Activity ◽  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Venous Occlusion ◽  
Clot Lysis ◽  
Lysis Time ◽  
Tissue Plasminogen ◽  
Euglobulin Clot Lysis Time ◽  
The Difference

To determine the origin of tissue plasminogen activator (t-PA) release during physical activity, we studied the separate and combined effects of venous occlusion and acute physical activity on t-PA release in arm and leg. In 15 healthy volunteers 20 min venous occlusions of arm and leg were performed simultaneously before physical activity ( maximal stress testing on treadmill)(occlusion I), immediately after physical activity and 45 min later (occlusion II). Blood samples were drawn from unoccluded arm before occlusion and after physical activity, and from occluded arm and leg after occlusion. Fibrinolytic activity was measured by euglobulin clot lysis time (ECLT) and t-PA activity assay. The amount of released t-PA during different stimuli (fibrinolytic potential) was calculated as the difference between post- and prestimulation fibrinolytic activity. Before physical activity there was a great increase in fibrinolytic activity due to t-PA in the occluded arm but no increase in the occluded leg. Physical activity itself caused a similar increase of systemic fibrinolytic activity as arm occlusion locally. After physical activity arm occlusion evoked equally good response than before it. Fibrinolytic activity during leg occlusion behaved differently: there was an increase in t-PA activity in the occluded leg which persisted one hour after physical activity, when systemic fibrinolytic activity already fell to initial level.These results demonstrated that walking and running triggered t-PA release from the leg vessels. Since leg occlusion was not a stimulus for t-PA release, it served only as a method to demonstrate the effect of physical activity.

scholarly journals The Lysis of Early Fibrin by Eosinophils

1977 ◽  
Author(s):  
Hau C. Kwaan ◽  
Ali A. Hatem
Keyword(s):  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Cell Membranes ◽  
Red Cell ◽  
Platelet Thrombus ◽  
Platelet Aggregates ◽  
Platelet Thrombi ◽  
Arteries And Veins ◽  
Morphologic Changes

This study examins the role of leukocytes within a thrombus by demonstrating the morphologic detail of their activities, the chemotactic properties of thrombi and the presence of plasminogen and possible plasminogen activator within eosinophils. A model which produces discrete, reproducible platelet thrombi in arteries and veins of dogs allowed timed studies of their early evolution. In this model, the growth of the thrombus was constantly monitored by a flowmeter and the thrombus could thus be removed at a selected period in its formation. It was then studied histologically for fibrin activity and also ultrastructually. Little fibrinolytic activity was found. In contrast to neutrophils which are concerned particularly with the phagocytosis and disruption of platelet aggregates, we observed that eosinophils participate in the lysis and disruption of the fibrin within these aggregates. The fibrin is rarely phagocytosed but is acted on at the surfaces of the eosinophils, usually in shallow invaginations of the cell membranes. The fibrin shows morphologic changes of lysis. It appears that eosinophils and neutrophils are concerned with the transformation of the early fibrin and platelet thrombus, rather than with the resolution of the formed, mainly fibrin and red cell thrombus.

Fibrinolytic activity before and after venous occlusion in thrombotic cases

1990 ◽  
Vol 4 ◽  
pp. 61
Author(s):  
J. Zamboulakis ◽  
C. Tsoukala ◽  
S. Liapi ◽  
T. Mandalaki
Keyword(s):  
Fibrinolytic Activity ◽  
Venous Occlusion ◽  
Before And After

Hypofibrinolysis in Patients with a History of Idiopathic Deep Vein Thrombosis and/or Pulmonary Embolism

1992 ◽  
Vol 67 (04) ◽  
pp. 397-401 ◽  
Author(s):  
Vito Grimaudo ◽  
Fedor Bachmann ◽  
Jacques Hauert ◽  
Maria-Adele Christe ◽  
Egbert K O Kruithof
Keyword(s):  
Pulmonary Embolism ◽  
Deep Vein Thrombosis ◽  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Venous Occlusion ◽  
Vein Thrombosis ◽  
Molar Excess ◽  
History Of ◽  
Deep Vein ◽  
Pai 1

SummaryAn impaired fibrinolytic activity after a venous occlusion test is the most common abnormality associated with thomboembolic disease. To better characterize the causes of abnormal responses we have measured different fibrinolytic parameters, before and after 10 and 20 min of venous occlusion, in 77 patients with a history of idiopathic deep vein thrombosis and/or pulmonary embolism and in 38 healthy volunteers.The patients had a lower mean fibrinolytic response to venous occlusion than the controls and higher antigen levels of tissue-type plasminogen activator (t-PA: Ag) and plasminogen activator inhibitor type 1 (PAI-1:Ag). Before venous occlusion, PAI-1 levels were at a molar excess over those of t-PA in all patients and controls. After 20 min of venous occlusion, the release of t-PA from the vascular endothelium resulted in a molar excess of t-PA over PAI-1 in the majority of controls (72%) but only in a minority of patients (39%).To identify patients with fibrinolytic abnormalities, reference intervals (RI) for fibrinolytic activity, t-PA:Ag and PAI-1:Ag were established in healthy controls. None of the patients had low levels of t-PA:Ag, but 17 (22%) had elevated PAI-1:Ag levels before venous occlusion and 12 (16%) exhibited low fibrinolytic activity after 20 min of venous occlusion. Ten of these were among the 17 subjects with high PAI-1: Ag levels before venous occlusion. Thus, the measurement of PAI-1:Ag levels before venous occlusion (i.e. in samples taken without any stimulation) is a sensitive (83%) and specific (89%) assay for the detection of patients with an impaired fibrinolytic response to venous occlusion.

Fibrinoyltic Activity of Human Carcinomas

1973 ◽  
Vol 30 (01) ◽  
pp. 133-137 ◽  
Author(s):  
Hans-Inge Peterson ◽  
Björn Petrusson ◽  
Kristoffer Korsan-Bengtsen
Keyword(s):  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Extraction Procedure ◽  
Malignant Tumour ◽  
Histochemical Method ◽  
Advantages And Disadvantages ◽  
Good Accordance ◽  
Human Carcinomas

SummaryThe fibrinolytic activity of 25 human malignant tumour specimens was studied by a semi-quantitative histochemical method and by a plasminogen activator extraction procedure. A good accordance between the results obtained with the two methods was found. The advantages and disadvantages of the methods are discussed.

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