A Micro-Method for the Determination of the Combined Activity of Factor II and VII (Prothrombin and Proconvertin)

1959 ◽  
Vol 03 (02) ◽  
pp. 297-301
Author(s):  
E. T Yin ◽  
N. J Senn
Keyword(s):  
Blood Plasma ◽  
Venous Blood ◽  
Capillary Blood ◽  
Dilution Curve ◽  
Blood Samples ◽  
Factor Ii ◽  
Flowing Blood ◽  
Finger Tip

SummaryA Micro-method for the determination of the combined activity of factors II and VII (prothrombin-proconvertin) based on the techniques of Owren and Koller et al. using capillary blood plasma, is being described. 0.18 ml of the free flowing blood from either the ear lobe or the finger tip is sucked into a micro-pipette previously containing 0.02 ml of anticoagulant. 0.05 ml of the centrifuged plasma is used to make a 1 : 10 dilution with buffered saline for the test. The clotting times in seconds are converted to percentages from the standard dilution curve plotted on double log. paper. Results obtained on over 300 capillary blood samples from in-patients receiving long term anticoagulant therapy were compared with those done by both Owren’s technique and the described micro-method on venous blood plasmas. These results were closely identical with each other.

Ferritin concentrations in plasma from capillary (finger prick) blood and venous blood compared.

1983 ◽  
Vol 29 (5) ◽  
pp. 871-873 ◽  
Author(s):  
L A Mejía ◽  
F E Viteri
Keyword(s):  
Blood Plasma ◽  
Close Association ◽  
Venous Blood ◽  
Capillary Blood ◽  
Ferritin Concentration ◽  
Antecubital Vein ◽  
Finger Prick ◽  
Adults And Children ◽  
Venous Plasma

Abstract Investigating the feasibility and validity of determining plasma ferritin concentration in blood obtained by finger prick, we studied 29 adults (ages 21-49 years) and 35 children (ages 14-66 months). Blood was sampled simultaneously in the same subject from both the antecubital vein (venous blood) and by finger pricking (capillary blood). The plasma was obtained by centrifugation. Ferritin concentration was determined by immunoradiometric analysis. Ferritin concentration in plasma from capillary blood was significantly higher than in venous plasma (p less than 0.01). This difference was more marked in children. The correlation between ferritin from the two blood sources was highly significant (r2 = 0.945 and 0.994 for samples from adults and children, respectively), and the slopes of the respective regression lines in both children and adults were significantly different from 1 (p less than 0.0001). We conclude that, despite the close association between the two procedures, the determination of ferritin concentration in capillary blood plasma overestimates the concentration of ferritin in venous blood plasma.

scholarly journals Comparison of capillary, venous and buffy coat blood samples in detecting Plasmodium species among malaria suspected patients attending at Hamusite health center. A cross-sectional study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Getu Abeje ◽  
Woyneshet Gelaye ◽  
Getaneh Alemu
Keyword(s):  
Health Center ◽  
Detection Rate ◽  
Venous Blood ◽  
Cross Sectional Study ◽  
Buffy Coat ◽  
Blood Film ◽  
Capillary Blood ◽  
Sectional Study ◽  
Cross Sectional ◽  
Blood Samples

Abstract Background Both capillary and venous blood samples have been interchangeably used for the diagnosis of malaria in Ethiopia. However, Plasmodium parasites are thought to be more concentrated in capillary than in venous blood. Hence, selecting a sample source where parasites are more concentrated is indispensable approach in order to maximize the accuracy of blood film microscopy. Therefore, the present study aimed to compare the detection rate and the parasitemia level of Plasmodium species from conventional capillary and venous blood films, and buffy coat preparations. Methods A facility based cross-sectional study was conducted from Feburary to March 2020 among 210 febrile patients attending Hamusite health center, northwest Ethiopia. Capillary and venous blood samples were collected and buffy coat was prepared from each sample. Thin and thick blood films were prepared, stained, and examined microscopically following standard protocol. Data were analysed using Statistical Package for Social Sciences Software version 20 and Med-Calc software version 19.3. Results Capillary blood buffy coat (61/210, 29.0%) had significantly higher detection rate as compared to capillary (48/210, 22.9%) and venous (42/210, 20.0%) blood films (p < 0.001). However, no significant difference was observed between capillary and venous blood films (p = 0.070) in detecting Plasmodium species. The highest and the lowest mean asexual stage parasite counts were found in capillary blood buffy coat (4692.88) and venous blood (631.43) films, respectively showing significant variations (p < 0.001). Mean gametocyte count was also highest in capillary blood buffy coat (3958.44). As compared to capillary blood buffy coat, the sensitivity of venous blood buffy coat, capillary blood film and venous blood film were 73.8, 78.7, 68.9%, respectively. Conclusion Capillary blood buffy coat samples showed the highest sensitivity in detecting and quantitating malaria parasites that its use should be promoted in clinical settings. However, conventional capillary and venous blood films could be used interchangeably.

Effects of Different Methods Used to Take Blood Samples on Blood Glucose Measurements

2021 ◽  
pp. 105477382110247
Author(s):  
Eda Ergin ◽  
Ayten Zaybak
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance Test ◽  
Venous Blood ◽  
Tolerance Test ◽  
Capillary Blood ◽  
Oral Glucose ◽  
Blood Samples ◽  
Significant Difference ◽  
Measurement Results ◽  
Quasi Experimental

The purpose of this study is to compare whether or not there is a difference between venous and capillary blood samples in blood glucose measurements and investigate the effects of different aseptic methods used in skin cleaning before collecting blood samples on measurement results. This quasi-experimental study was conducted with 109 patients. The capillary first and second blood drop values taken from the patients after fasting and at 2 hours following 75 g oral glucose tolerance test (OGTT) and capillary and venous blood glucose values were compared. There was no significant difference between the median venous blood glucose value and the capillary second blood drop value taken after wiping the finger with alcohol. There was no significant difference between the first and second blood drop values of capillary blood glucose 2 hours after OGTT.

LRF and TRF test during long-term danazol treatment: increase of the LH and FSH responses but decrease of the prolactin and TSH responses

1983 ◽  
Vol 104 (1) ◽  
pp. 1-5 ◽  
Author(s):  
J. Leppäluoto ◽  
L. Rönnberg ◽  
P. Ylöstalo
Keyword(s):  
Clinical Symptoms ◽  
Follicular Phase ◽  
Blood Samples ◽  
Hormone Levels ◽  
Coefficients Of Variation ◽  
The Mean ◽  
Thyroid Hormone Levels ◽  
Low Serum

Abstract. Seven patients suffering from severe endometriosis were treated with danazol 200 mg × 3 daily for 6 months. Clinical symptoms were alleviated and menses disappeared in response to the treatment. After cessation of the treatment the menstrual bleedings returned in 1–3 months. Blood samples for determination of gonadotrophins, prolactin (Prl), oestradiol (E2), progesterone, thyroid hormones and thyrotrophin in radioimmunoassays were taken and a combined TRF and LRF test carried out in the follicular phase before treatment, at the 6th month of treatment and after reappearance of the first menses. There were no statistically significant changes in the basal levels of serum FSH, LH or TSH during the danazol treatment. Neither was there any change in episodic secretions of FSH, LH or Prl, as determined by the mean coefficients of variation of the hormone levels in seven consecutive samples taken at 20 min intervals. On the other hand, serum E2, Prl and thyroid hormone levels were significantly decreased in the 6th month of treatment. In the TRF-LRF test the responses of serum FSH and LH were significantly higher and those of serum Prl and TSH significantly lower during danazol treatment than before. Prl responses remained lowered after the treatment. It appears that low serum oestrogen levels, induced by the danazol treatment, sensitize the pituitary gonadotrophs to exogenous LRF, but make the sensitivity of thyrotrophs and lactotrophs lower to exogenous TRF. These results thus indicate that danazol does not make the pituitary gonadotrophs insensitive to LRF, but danazol may rather inhibit the secretion of hypothalamic LRF.

scholarly journals Blood Glucose Assay Using Dextrostix and a Reflectance Meter

1972 ◽  
Vol 9 (1-6) ◽  
pp. 91-94 ◽  
Author(s):  
I. W. Percy-Robb ◽  
R. S. McMaster ◽  
A. D. B. Harrower ◽  
L. J. P. Duncan
Keyword(s):  
Blood Glucose ◽  
Venous Blood ◽  
Reference Method ◽  
Capillary Blood ◽  
Blood Samples ◽  
Laboratory Staff ◽  
Glucose Assay ◽  
Reflectance Meter ◽  
High Degree ◽  
Blood Glucose Concentrations

The ‘Dextrostix’-reflectance meter system for blood glucose analysis has been evaluated using a blood glucose reference method. A high degree of concordance between the two methods was obtained when analyses were performed by skilled laboratory staff on venous blood samples containing fluoride, with a 75 s contact time. Skilled laboratory staff performed significantly better than unskilled staff. Capillary blood glucose concentrations correlated poorly with concentrations in venous blood samples taken at the same time as the capillary blood.

scholarly journals Great Obstetrical Syndromes In G20210a Mutation Carriers Associated With High Prothrombin Activity

2020 ◽  
Author(s):  
Mariya Nikolaeva ◽  
Andrey Pavlovich Momot ◽  
Marina Sabirovna Zainulina ◽  
Natalia Nikolaevna Yasafova ◽  
Irina Alekseevna Taranenko
Keyword(s):  
Blood Plasma ◽  
Venous Blood ◽  
Threshold Value ◽  
Main Group ◽  
Trophoblast Invasion ◽  
Control Group ◽  
G20210a Mutation ◽  
Factor Ii ◽  
Invasion Waves ◽  
In Blood Plasma

Abstract Objective: to study the association between high activity of Factor II (prothrombin) in blood plasma with G20210A mutation and the development of great obstetrical syndromes.Material and methods: A prospective clinical cohort study was conducted on 290 pregnant women (average age 31.7±4.7 years old). The main group was made up of 140 G20210A patients, while the control group comprised 150 women with the wild G20210G type. The aim was to evaluate the activity of Factor II in the venous blood plasma during the stages of pregnancy with regard to trophoblast invasion waves. As per results, association analysis of Factor II activity value and gestational complications was carried out.Results: In the control group, the median (Me) of Factor II activity ranged from 108% (preconception period) to 144% (pregnancy) [95% CI 130-150]. In patients with the GA type, the value was significantly higher in related periods, ranging from 149% to 181% [95% CI 142-195], p<0.0001. With Factor II activity ranging from 148.5% to 180.6%, pregnancies in the main group had no complications. Higher levels of Factor II activity were associated with the development of early and/or severe preeclampsia (PE) and fetal growth retardation (FGR).Conclusion: The data obtained regarding Factor II activity in blood plasma, juxtaposed with the development of great obstetrical syndromes, allow to assume that manifestation of G20210A in early and/or severe PE and FGR is associated with this coagulation factor's level of activity. Threshold value of the Factor II activity with G20210A mutation, allowing to predict the development of PE, comprised 171.0% at the preconception stage (AUC – 0.86; p<0.0001) and within 7-8 weeks of gestation it was 181.3% (AUC – 0.84; p<0.0001).

scholarly journals Use of Capillary Blood Samples Leads to Higher Parasitemia Estimates and Higher Diagnostic Sensitivity of Microscopic and Molecular Diagnostics of Malaria Than Venous Blood Samples

2018 ◽  
Vol 218 (8) ◽  
pp. 1296-1305 ◽  
Author(s):  
Johannes Mischlinger ◽  
Paul Pitzinger ◽  
Luzia Veletzky ◽  
Mirjam Groger ◽  
Rella Zoleko-Manego ◽  
...  
Keyword(s):  
Molecular Diagnostics ◽  
Venous Blood ◽  
Capillary Blood ◽  
Diagnostic Sensitivity ◽  
Blood Samples

Comparison of capillary blood versus venous blood samples in the assessment of immunity to measles

1996 ◽  
Vol 61 (1-2) ◽  
pp. 73-77 ◽  
Author(s):  
F. Novello ◽  
B. Ridolfi ◽  
L. Fiore ◽  
G. Buttinelli ◽  
E. Medda ◽  
...  
Keyword(s):  
Venous Blood ◽  
Capillary Blood ◽  
Blood Samples

Accuracy and precision of analyses for total cholesterol as measured with the Reflotron cholesterol method.

1989 ◽  
Vol 35 (8) ◽  
pp. 1734-1739 ◽  
Author(s):  
P S Bachorik ◽  
R H Bradford ◽  
T Cole ◽  
I Frantz ◽  
A M Gotto ◽  
...  
Keyword(s):  
Plasma Cholesterol ◽  
Venous Blood ◽  
Reference Method ◽  
Capillary Blood ◽  
Average Difference ◽  
Blood Samples ◽  
Laboratory Values ◽  
Accuracy And Precision ◽  
Single Finger ◽  
Made In

Abstract We compared plasma cholesterol measurements made with the Boehringer Mannheim Reflotron reflectance photometric analyzer in 1298 capillary blood samples with measurements made in venous blood samples collected at the same time and analyzed in four standardized Lipid Research Clinics laboratories. The Reflotron measurements averaged 0.8% to 7.8% lower than the laboratory values. Correlations (r) between the two sets of measurements ranged from 0.92 to 0.96. In some samples, however, the Reflotron values differed from the laboratory values by greater than or equal to 12%; the cholesterol concentrations in these samples tended to be higher than in those for which better agreement was observed. The smaller negative biases were observed when test strips were used that were calibrated with reference to the Centers for Disease Control Reference Method for cholesterol. The agreement between sequential Reflotron values averaged less than or equal to 4.3%. There was an average difference of less than or equal to 1.0% between Reflotron measurements made in each of two sequential capillary blood samples taken from a single finger puncture.

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