scholarly journals Evaluation of Erythrocytes Magnetized Technology for Measurement of ABO Isoagglutinin Titers

2021 ◽  
Author(s):  
Parul Chopra ◽  
Sunanda Bhardwaj ◽  
Ajay Samkaria ◽  
Asha Amoli ◽  
Anil Arora
Keyword(s):  
Blood Group ◽  
Antibody Titer ◽  
Immunoglobulin G ◽  
Patient Management ◽  
Immunoglobulin M ◽  
Reference Laboratory ◽  
Microsoft Excel ◽  
Consistent Method ◽  
Automated Instrument

Abstract Background A variation in the measurement of ABO antibody titer has been seen among different laboratories due to lack of standardization. In our study, we aim to evaluate automated ABO isoagglutinin titer measurements by erythrocytes magnetized technology (EMT) and compare with conventional tube technique (TT). Methods We performed ABO isoagglutinin titration on samples received in a reference laboratory during a period of 2 months. A total of 134 tests for immunoglobulin G (IgG) titer and 116 for immunoglobulin M (IgM) for anti-A or anti-B were included in the study. Samples were processed for ABO isoagglutination titers by both TT and EMT by QWALYS-3 (Diagast, France). Microsoft Excel was used to compile data, for all calculations, and to draw graphs and plots. The number and percentage of cases within ±1, ±2, or ±3 titer difference (TT-EMT) were calculated. Results Median titers and their ranges obtained by EMT were higher or equal to those by TT for all IgM and IgG ABO-antibodies in all blood group (BGs), except anti-A IgM in (BG) O that was lower by EMT (32 [4:128]) than TT (48 [8:256]). One twenty one (121/134, 90.3%) cases of IgG titer showed an agreement by both methods (within ± one titer difference). One hundred seven cases (107/116, 92.2%) for IgM titer were within one titer difference by both the methods. Conclusion Results of titration by EMT-based automated instrument QWALYS-3 and conventional TT may vary by one titer dilution in the majority of cases. Use of consistent method for patient management is, therefore, advised.

Detection of Immunoglobulin M and Immunoglobulin G Antibodies Against Orientia tsutsugamushi for Scrub Typhus Diagnosis and Serosurvey in Endemic Regions

2020 ◽  
Vol 57 (12) ◽  
pp. 1131-1134
Author(s):  
Mohan D. Gupte ◽  
Manish Gupte ◽  
Suchit Kamble ◽  
Arati Mane ◽  
Suvarna Sane ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Scrub Typhus ◽  
Immunoglobulin M ◽  
Orientia Tsutsugamushi

scholarly journals Seroprevalence of Severe Acute Respiratory Syndrome-Coronavirus-2 Immunoglobulin M and Immunoglobulin G Antibodies among the Population of Koya University

2021 ◽  
Vol 9 (2) ◽  
pp. 51-57
Author(s):  
Safia S. I. Blbas ◽  
Hiwa A. Ahmad ◽  
Dawan J. Hawezy ◽  
Hemn Shawgery ◽  
Hersh N. Bahadin
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Infection Rate ◽  
Immunoglobulin G ◽  
Prevalence Rate ◽  
Complete Blood Count ◽  
Immunoglobulin M ◽  
Teaching Staff ◽  
Serological Detection ◽  
Population Total ◽  
Elisa Technique

Coronavirus is a pandemic disease. In most cases, the exact infection rate cannot be determined as not everybody can be tested for the virus, even though some of them carry the virus silently. Therefore, detection of antibodies of this virus is more practical to give us a better clue about the rate of infection because the asymptomatic people can be tested too. The serological detection of anti-Severe Acute Respiratory Syndrome-Coronavirus (SARS-COV-2) antibodies among asymptomatic and moderate symptomatic individuals gives us the vital point to understanding the prevalence rate of COVID-19 among the population. Total of (436) volunteers were participated, (96) from teaching staff, (172) employee, and (168) students. Anti-SARS-COV-2 immunoglobulin G (IgG) and Immunoglobulin M (IgM) were detected in the serum by ELISA technique, and complete blood count was performed for all participants. The number of seropositive of anti-SARS-COV-2/IgG was (159), whereas IgM was (66). The highest prevalence rate of IgG detected among participants with family member infected with coronavirus (42.7%). Total WBCs count significantly increased among IgM positive participants. Many asymptomatic people were infected with coronavirus, which lead to more spreading of the virus among the population. Therefore, mass screening of the population for specific antibody against coronavirus is important to reduce the infection rate.

Serodiagnosis of viral hepatitis A by a modified competitive binding radioimmunoassay for immunoglobulin M anti-hepatitis A virus

1979 ◽  
Vol 9 (1) ◽  
pp. 120-127
Author(s):  
D W Bradley ◽  
H A Fields ◽  
K A McCaustland ◽  
J E Maynard ◽  
R H Decker ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Protein A ◽  
Competitive Binding ◽  
Immunoglobulin M ◽  
Test Results ◽  
Solid Phase Radioimmunoassay ◽  
Viral Hepatitis A

A competitive binding radioimmunoassay (CBA) for antibody to hepatitis A virus (HAV) was evaluated and compared with a standard solid-phase radioimmunoassay for anti-HAV, CBA was found to be sensitive and specific for the detection of anti-HAV, as demonstrated by the 98% concordance of CBA and solid-phase radioimmunoassay test results. The standard CBA test was modified for the differential detection of acute (immunoglobulin M) and convalescent (immunoglobulin G) anti-HAV by incorporation of a step in which immunoglobulin G anti-HAV was preferentially absorbed with S. aureus cells (protein A). The modified CBA test was shown to be capable of differentiating between acute- and convalescent-phase sera. The modified CBAM test was able to detect immunoglobulin M anti-HAV up to approximately 4 weeks after the onset of illness.

scholarly journals Antiphosphatidylserine Immunoglobulin M and Immunoglobulin G Antibodies Are Higher in Vivax Than Falciparum Malaria, and Associated With Early Anemia in Both Species

2019 ◽  
Vol 220 (9) ◽  
pp. 1435-1443 ◽  
Author(s):  
Bridget E Barber ◽  
Matthew J Grigg ◽  
Kim Piera ◽  
Fiona H Amante ◽  
Timothy William ◽  
...  
Keyword(s):  
Falciparum Malaria ◽  
Immunoglobulin G ◽  
Vivax Malaria ◽  
Immunoglobulin M

Anti-phosphatidylserine antibodies (PS-Abs) are elevated in Malaysian patients with vivax and falciparum malaria and are highest in vivax malaria. In vivax and falciparum malaria, PS-Abs correlate inversely with admission and nadir hemoglobin, suggesting that PS-Abs contribute to anemia from these species.

Triple positivity for nonstructural antigen 1, immunoglobulin M and immunoglobulin G is predictive of severe thrombocytopaenia related to dengue infection

2020 ◽  
Vol 129 ◽  
pp. 104509
Author(s):  
Sanjay de Mel ◽  
Basuru Uvindu Thilakawardana ◽  
Primesh de Mel ◽  
Choong Shi Hui Clarice ◽  
Malka Shalindi ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Dengue Infection ◽  
Immunoglobulin M

Major Immunoglobulin Ratios in Carcinoma of the Head and Neck

1978 ◽  
Vol 87 (3) ◽  
pp. 412-415 ◽  
Author(s):  
Arnold E. Katz ◽  
John O. Nysather ◽  
Lee A. Harker
Keyword(s):  
Head And Neck ◽  
Immunoglobulin G ◽  
Immunoglobulin A ◽  
Elevated Serum ◽  
Immunoglobulin M ◽  
Serum Immunoglobulin ◽  
Control Groups ◽  
Immunoglobulin Production ◽  
Patients With Cancer ◽  
Serum Iga

— Serum immunoglobulin A (IgA), immunoglobulin G (IgG), and immunoglobulin M (IgM) levels were determined on 245 patients with carcinoma of the head and neck and on 92 controls. Ratios of these levels were calculated for each subject. The patients with cancer demonstrated elevated serum IgA levels ( P <.0001) and elevated IgA/IgM and IgA/IgG ratios ( P <.05). No differences were noted when the IgM/IgG ratios were compared between the cancer and the control groups. These observations are offered as evidence that previously reported elevations of serum IgA levels in patients with carcinoma of the head and neck are not merely an index of nonspecific increased immunoglobulin production in these patients.

scholarly journals Significance of a Positive Toxoplasma Immunoglobulin M Test Result in the United States

2015 ◽  
Vol 53 (11) ◽  
pp. 3601-3605 ◽  
Author(s):  
Reshika Dhakal ◽  
Kiran Gajurel ◽  
Christelle Pomares ◽  
Jeanne Talucod ◽  
Cynthia J. Press ◽  
...  
Keyword(s):  
United States ◽  
Chronic Infection ◽  
Serological Test ◽  
Acute Infection ◽  
The United States ◽  
Immunoglobulin M ◽  
Reference Laboratory ◽  
Test Results ◽  
Chronic Infections ◽  
Content Type

A positiveToxoplasmaimmunoglobulin M (IgM) result is often interpreted as a marker of an acute infection. However, IgM can persist for several years, andToxoplasmacommercial IgM diagnostic test kits can yield a number of false-positive results. For these reasons, a chronicToxoplasmainfection can be erroneously classified as an acute infection, resulting in serious adverse consequences, especially in pregnant women, leading to emotional distress and unnecessary interventions, including termination of pregnancy. Interpretation ofToxoplasmaserology at a reference laboratory can help differentiate a recently acquired infection from a chronic infection. Serological test results for 451 patients with positiveToxoplasmaIgM and IgG test results obtained at nonreference laboratories (NRLs) that were referred to Palo Alto Medical Foundation Toxoplasma Serology Laboratory (PAMF-TSL) to determine whether the patient was acutely or chronically infected were retrospectively reviewed. PAMF-TSL results established that of the 451 patients, 335 (74%) had a chronic infection, 100 (22%) had an acute infection, and 7 (2%) were not infected, and for 9 (2%), results were indeterminate. PositiveToxoplasmaIgM and IgG test results obtained at NRLs cannot accurately distinguish between acute and chronic infections. To do so, testing at reference laboratories is required, as mandated in 1997 in a letter from the Food and Drug Administration (FDA) to clinicians and laboratories in the United States.

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