scholarly journals Application of Photoacoustic Imaging for Lymphedema Treatment

2021 ◽  
Author(s):  
Yushi Suzuki ◽  
Hiroki Kajita ◽  
Shiho Watanabe ◽  
Keisuke Okabe ◽  
Hisashi Sakuma ◽  
...  
Keyword(s):  
High Resolution ◽  
Imaging System ◽  
Photoacoustic Imaging ◽  
Three Dimensional ◽  
Lymphatic Vessels ◽  
Small Diameter ◽  
Detailed Structure ◽  
High Resolution Images ◽  
Major Application ◽  
Lymph Fluid

Abstract Background Lymphatic vessels are difficult to identify using existing modalities as because of their small diameter and the transparency of the lymph fluid flowing through them. Methods Here, we introduce photoacoustic lymphangiography (PAL), a new modality widely used for lymphedema treatment, to observe limb lymphatic vessels. The photoacoustic imaging system used in this study can simultaneously visualize lymphatic vessels and veins with a high resolution (0.2 mm) and can also observe their three-dimensional relationship with each other. Results High-resolution images of the lymphatic vessels, detailed structure of the dermal back flow, and the three-dimensional positional relationship between the lymphatic vessels and veins were observed by PAL. Conclusion The clear image provided by PAL could have a major application in pre- and postoperative use during lymphaticovenular anastomosis for lymphedema treatment.

Electron crystallographic determination of the 3-D structure of staurolite at atomic resolution

1991 ◽  
Vol 49 ◽  
pp. 540-541
Author(s):  
Kenneth H. Downing ◽  
Hu Meisheng ◽  
Hans-Rudolf Went ◽  
Michael A. O'Keefe
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
High Resolution Electron Microscopy ◽  
Atomic Resolution ◽  
Dimensional Structure ◽  
Structure Information ◽  
Resolution Electron ◽  
Scattering Effects ◽  
High Resolution Images ◽  
Effects Of Radiation

With current advances in electron microscope design, high resolution electron microscopy has become routine, and point resolutions of better than 2Å have been obtained in images of many inorganic crystals. Although this resolution is sufficient to resolve interatomic spacings, interpretation generally requires comparison of experimental images with calculations. Since the images are two-dimensional representations of projections of the full three-dimensional structure, information is invariably lost in the overlapping images of atoms at various heights. The technique of electron crystallography, in which information from several views of a crystal is combined, has been developed to obtain three-dimensional information on proteins. The resolution in images of proteins is severely limited by effects of radiation damage. In principle, atomic-resolution, 3D reconstructions should be obtainable from specimens that are resistant to damage. The most serious problem would appear to be in obtaining high-resolution images from areas that are thin enough that dynamical scattering effects can be ignored.

High-resolution transmission electron microscopic investigations of molybdenum thin films on faceted α-Al2O3

2005 ◽  
Vol 38 (2) ◽  
pp. 260-265 ◽  
Author(s):  
Leonore Wiehl ◽  
Jens Oster ◽  
Michael Huth
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
Epitaxial Relationship ◽  
Electron Microscopic ◽  
Force Microscopy ◽  
Atomic Force ◽  
Transmission Electron ◽  
High Resolution Images ◽  
Α Al2o3 ◽  
Relationship Of

Epitaxially grown Mo films on a faceted corundum (α-Al2O3)mplane were investigated by transmission electron microscopy. Low- and high-resolution images were taken from a cross-section specimen cut perpendicular to the facets. It was possible to identify unambiguously the crystallographic orientation of these facets and explain the considerable deviation (∼10°) of the experimental interfacet angle, as measured with atomic force microscopy (AFM), from the expected value. For the first time, proof is given for a smooth \{10\bar{1}1\} facet and a curvy facet with orientation near to \{10\bar{1}\bar{2}\}. Moreover, the three-dimensional epitaxial relationship of an Mo film on a faceted corundummsurface was determined.

scholarly journals Line of Sight and Image Motion Compensation for Step and Stare Imaging System

2020 ◽  
Vol 10 (20) ◽  
pp. 7119
Author(s):  
Jihong Xiu ◽  
Pu Huang ◽  
Jun Li ◽  
Hongwen Zhang ◽  
Youyi Li
Keyword(s):  
High Resolution ◽  
Motion Compensation ◽  
Imaging System ◽  
Control Flow ◽  
Image Motion ◽  
Line Of Sight ◽  
Pointing Accuracy ◽  
High Resolution Images ◽  
Pointing Control ◽  
And Control

In recent years, applications such as marine search and rescue, border patrol, etc. require electro-optical equipment to have both high resolution and precise geographic positioning abilities. The step and stare working based on a composite control system is a preferred solution. This paper proposed a step and stare system composed of two single-axis fast steering mirrors and a two-axis gimbal. The fast steering mirrors (FSMs) realize image motion compensation and the gimbal completes pointing control. The working principle and the working mode of the system are described first. According to the imaging optical path, the algorithm and control flow of the line of sight (LOS) and image motion compensation are developed. The proposed method is verified through ground imaging and flight tests. Under the condition of flight, the pointing accuracy of the target can be controlled within 15 m. The proposed algorithm can achieve effective motion compensation and get high-resolution images. This achieves high resolution and accurate LOS simultaneously.

A System for Computer-based Reconstruction of 3-Dimensional Structures from Serial Tissue Sections: an Application to the Study of Normal and Neoplastic Mammary Gland Biology

2001 ◽  
Vol 7 (S2) ◽  
pp. 964-965
Author(s):  
Rodrigo Fernandez-Gonzalez ◽  
Arthur Jones ◽  
Enrique Garcia-Rodriguez ◽  
Davis Knowles ◽  
Damir Sudar ◽  
...  
Keyword(s):  
Mammary Gland ◽  
High Resolution ◽  
Imaging System ◽  
Contextual Information ◽  
Three Dimensional ◽  
Tissue Level ◽  
Disease Heterogeneity ◽  
Tissue Sections ◽  
Virtual Reconstruction ◽  
Analytical Microscopy

Tissue heterogeneity and three-dimensionality are generally neglected by most traditional analytical microscopy methods in Biology. These often disregard contextual information important for understanding most biological systems. in breast cancer, which is a tissue level disease, heterogeneity and three dimensionality are at the very base of cancer initiation and clonal progression. Thus, a three dimensional quantitative system that allows low resolution virtual reconstruction of the mammary gland from serial sections, followed by high resolution cell-level reconstruction and quantitative analysis of the ductal epithelium emerges as an essential tool in studying the disease. We present here a distributed microscopic imaging system which allows acquiring and registering low magnification (1 pixel = 5 μm) conventional (bright field or fluorescence) images of entire tissue sections; then it allows tracing (in 3D) the ducts of the mammary gland from adjacent sections, to create a 3D virtual reconstruction of the gland; finally it allows revisiting areas of interest for high resolution (1 pixel = 0.5 μm) imaging and automatic analysis. We illustrate the use of the system for the reconstruction of a small volume of breast tissue.

High-resolution X-ray topographic images of dislocations in a silicon crystal recorded using an X-ray zooming tube

1998 ◽  
Vol 5 (3) ◽  
pp. 1079-1081
Author(s):  
Shigeru Kimura ◽  
Tatsuya Matsumura ◽  
Katsuyuki Kinoshita ◽  
Keiichi Hirano ◽  
Hiroshi Kihara
Keyword(s):  
High Resolution ◽  
Digital Imaging ◽  
Imaging System ◽  
Silicon Crystal ◽  
Stage Structure ◽  
Magnification Factor ◽  
Double Crystal ◽  
X Ray ◽  
Imaging Detector ◽  
High Resolution Images

A Be-window-type X-ray zooming tube is an X-ray digital imaging system whose magnification factor of X-ray images can be easily varied from 10 to 200, and whose spatial resolution is less than 0.5 µm. This zooming tube was used as an imaging detector in double-crystal X-ray topography to obtain high-resolution images of dislocations in a silicon crystal. X-ray interference images of about 5 µm were observed even though optimal performance of the X-ray zooming tube could not be achieved. The results indicate that the X-ray zooming tube might make a good detector for X-ray topography with minor improvements in its stage structure.

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