Pharmacological Study of Santolina chamaecyparissus. I. Acute Toxicity, Antiinflammatory and Antiulcer Activity

For over a century, peptic ulcer has been one of the most common gastrointestinal tract (GIT) disorder. There are number of drugs are now available for treatment. Drugs of herbal origin reduce the offensive factors and have proved to be safe, clinically effective, relatively less expensive, globally competitive, and with better patient tolerance.This study was performed to assess the anti-ulcer activity on different parts of B.aristata. Apart from that, acute toxicity, qualitative chemical analysis, total phenolic content (TPC), total flavonoid content(TFC) and in vitro antioxidant activities were evaluated. The potentially active plant part was selected for screening as gastro protective, in vivo antioxidant and antisecretory activities in ulcerated rats.The 50% ethanolic extract of B. aristata were subjected to preliminary phytochemical screening, estimation of TFC and TPC. The crude extract from the leaves of B. aristata gave best antiulcer activity among flower and stem. In acute toxicity studies, the administration of the crude extract of B. aristata leaves did not reveal any adverse effects or toxicity in rats at fourteen days observations.The results of these studies have shown that ethylexract of B.aristata leaf (EEBAL) produced a significant dose dependent ulcerprotective, antioxidant and antisecretory activity by blocking the activity of proton pump, protecting from antioxidants produced during stress induced ulcer and by enhancing glycoprotein levels.

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Pharmacological study on the acute toxicity of isoproterenol (ISO) in miceM. Noyer, M. Vandevelde, M. Baldewijns and L. Brouwers. UCB-DIPHA, Biochemistry Department, 68, rue Berkendael, B-1060 Bruxelles, Belgium

Journal of Molecular and Cellular Cardiology ◽

10.1016/0022-2828(83)90987-2 ◽

1983 ◽

Vol 15 ◽

pp. 72-72

Keyword(s):

Acute Toxicity ◽

Pharmacological Study

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GC-MS, FTIR and Antiulcer Screening of Aqueous Seed Extract and Oil of Nigella sativa in Wistar Rats

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v11i6.5036 ◽

2021 ◽

Vol 11 (6) ◽

pp. 48-60

Author(s):

S.O. Bruce ◽

O.I. Nwafor ◽

M.A. Omoirri ◽

N.M. Adione ◽

I.P. Onyeka ◽

...

Keyword(s):

Acute Toxicity ◽

Aqueous Extract ◽

Wistar Rats ◽

Octadecadienoic Acid ◽

Nigella Sativa ◽

Safety Margin ◽

Seed Extract ◽

Antiulcer Activity ◽

Black Seed ◽

Oil Extract

Peptic ulcer disease (PUD) is a sore in the lining of the stomach or duodenal mucosa. This study was aimed at evaluating the antiulcer activity of the aqueous extract of Nigella sativa (black seed) and its constitutents. The pharmacognostic properties of the dry seeds were determined. The seeds were extracted using two methods digestion and hydro-distillation. The acute toxicity, phytochemical constituents and the antiulcer evaluation were performed on ethanol-induced ulcer in wistar rats using a standard method. FTIR and GC-MS analysis of the aqueous seed extract was also determined using standard methods. Nigella sativa seed shows the presence of starch grains, lignified tissues, tannins, cellulose, protein and oil globules. The aqueous extract has a high safety margin. The phytochemical studies revealed the presence of saponins, flavonoids, alkaloids, tannins, glycosides, fats and oil. The black seed aqueous and oil extract at 500 mg/kg significantly reduced the acidity, total acidity, and ulcer index, and pH of gastric content when compared with the positive control (Famotidine). The FTIR analysis identified the presence of the following functional groups chloro, ether, amine, carboxylic acid, nitriles, methylene, alcohol, while the GC-MS identified five compounds such as glycerin, n-Hexadecanoic acid, 9, 12-octadecadienoic acid-methyl ester, 9, 12-octadecadienoic acid and 9, 12-octadecadienoyl chloride. The pharmacognostic properties can act as a reliable tool for the standardization of the plant part. This study suggests that aqueous and oil extract possess antiulcer properties. Thus the aqueous and oil extract of black seed can be considered as antiulcer medication traditionally. Keywords: Nigella sativa, Black seed, Pharmacognostic, Phytochemical, Acute toxicity (LD50), Antiulcer.

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Pharmacological study of Kako-bushi-matsu: Analgesic action and acute toxicity.

Folia Pharmacologica Japonica ◽

10.1254/fpj.94.309 ◽

1989 ◽

Vol 94 (5) ◽

pp. 309-317 ◽

Cited By ~ 1

Author(s):

Mitsuo MURAYAMA ◽

Yoshinori NAMIKI

Keyword(s):

Acute Toxicity ◽

Pharmacological Study ◽

Analgesic Action

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A Pharmacological Study of Gastric Antiulcer Activity of the Leaf Extracts of Moringa oleifera Linn

Acta Scientific Pharmaceutical Sciences ◽

10.31080/asps.2021.05.0765 ◽

2021 ◽

Vol 5 (8) ◽

pp. 43-50

Author(s):

Dibyajyoti Deka ◽

Mangala Lahkar ◽

Himadri Sekhar Dasgupta ◽

Dhriti KR Brahma ◽

Tirtha Chaliha

Keyword(s):

Moringa Oleifera ◽

Pharmacological Study ◽

Antiulcer Activity ◽

Leaf Extracts

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Hydromethanolic Crude Extract of the Leaf of Urtica simensis Hochst. ex. A. Rich. (Urticaceae) Acquires Appreciable Antiulcer Effect: Validation for In Vivo Antiulcer Activity

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/6591070 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Woretaw Sisay ◽

Yared Andargie ◽

Mulugeta Molla ◽

Alefe Norahun

Keyword(s):

Acute Toxicity ◽

Crude Extract ◽

Ulcer Healing ◽

Negative Control ◽

Antiulcer Activity ◽

Dependent Manner ◽

Ulcer Disease ◽

Ulcer Model ◽

Oral Acute Toxicity

Background. Urtica simensis has been used for the treatment of peptic ulcer disease in Ethiopian folkloric medicine by drinking its juice after boiling the semicrushed leaf. To our latest understanding, no in vivo study was available regarding its antiulcer activity. The present study was done to appraise the ulcer-protective and ulcer healing activity of hydromethanolic crude extract of leaf of U. simensis in rats. Methods. Preliminary qualitative phytochemical screening and oral acute toxicity were carried out using a standard protocol. To validate U. simensis in vivo antiulcer potential pyloric ligature, cold restraint stress and acetic acid-induced ulcer models were employed. The extracts (100, 200, and 400 mg per kg of body weight per day), standard treatment (omeprazole 20 mg/kg/day), and vehicle (distilled water 10 ml/kg/day) were given to treatment, positive, and negative controls by oral gavage, respectively. Parameters were then evaluated accordingly after the humane scarification of rats. Results. Any sign of toxicity was not observed in the oral acute toxicity test. The crude extracts exerted a significant ( P < 0.05 ) inhibition of ulcer risk compared to the negative control. In the pylorus ligation-induced ulcer model, its antisecretory activity was in a dose-dependent manner. The highest gastroprotective effect (67.68%) was exhibited by the 400 mg/kg/day dose of 80% methanolic crude extract. Regarding the chronic ulcer model, treatment at a dosage of 100, 200, and 400 mg/kg/day cures ulcers by 33.54%, 58.33%, and 67.07%, respectively, as compared to the negative control groups remarkably. Conclusion. The findings of the present study confirmed the safety and a promising in vivo ulcer healing and antiulcerogenic activity of U. simensis, thus supporting the traditional claim. In-depth investigations on the plant, however, are highly recommended.

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