DNA damage in oral cancer cells induced by nitrogen atmospheric pressure plasma jets

AbstractRecently, many different plasma sources are being investigated for exhaust gases treatment, odor abatement, VOC removal, soil conditioning, surface decontamination or tissue disinfection and sterilization. Among many different plasma reactors investigated in laboratories, gliding arc discharges (GAD), dielectric barrier discharges (DBD), pulsed discharges (PD), atmospheric pressure glow discharges (APGD) and atmospheric pressure plasma jets (APPJ) seem to be the most promising for high pressure low temperature applications. They can be designed as multi-electrodes’ high power system that can be used in environment protection processes, like decontamination of large surfaces and treatment of large volume of polluted gases, as well as small size and low power devices for biomedical applications, like plasma healing, disinfection and sterilization. Paper presents review of power supply systems for cold plasma reactors. Dielectric Barrier Discharge (DBD), Gliding Arc Discharge (GAD) and atmospheric pressure plasma jet (APPJ) reactors with their supply systems have been discussed from the point view of their characteristics, possibility to control power to the discharge and efficiency. Taking into account the plasma reactor characteristics and nature (nonlinear resistive and/or capacitive) different solutions of power suppliers have been presented: transformer type, AC/DC/AC inverter, RF-frequency system and frequency resonant inverter.

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Inactivation of microbes and macromolecules by atmospheric-pressure plasma jets

Applied Microbiology and Biotechnology ◽

10.1007/s00253-014-5781-9 ◽

2014 ◽

Vol 98 (14) ◽

pp. 6205-6213 ◽

Cited By ~ 52

Author(s):

Jan-Wilm Lackmann ◽

Julia Elisabeth Bandow

Keyword(s):

Atmospheric Pressure ◽

Atmospheric Pressure Plasma ◽

Plasma Jets ◽

Pressure Plasma

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Large-Scale Image Analysis for Investigating Spatio-Temporal Changes in Nuclear DNA Damage Caused by Nitrogen Atmospheric Pressure Plasma Jets

International Journal of Molecular Sciences ◽

10.3390/ijms21114127 ◽

2020 ◽

Vol 21 (11) ◽

pp. 4127

Author(s):

Xu Han ◽

James Kapaldo ◽

Yueying Liu ◽

M. Sharon Stack ◽

Elahe Alizadeh ◽

...

Keyword(s):

Cell Cycle ◽

Dna Damage ◽

Image Analysis ◽

Cancer Cells ◽

Atmospheric Pressure ◽

Large Scale ◽

Nuclear Dna ◽

Atmospheric Pressure Plasma ◽

Plasma Jet ◽

Spatio Temporal

The effective clinical application of atmospheric pressure plasma jet (APPJ) treatments requires a well-founded methodology that can describe the interactions between the plasma jet and a treated sample and the temporal and spatial changes that result from the treatment. In this study, we developed a large-scale image analysis method to identify the cell-cycle stage and quantify damage to nuclear DNA in single cells. The method was then tested and used to examine spatio-temporal distributions of nuclear DNA damage in two cell lines from the same anatomic location, namely the oral cavity, after treatment with a nitrogen APPJ. One cell line was malignant, and the other, nonmalignant. The results showed that DNA damage in cancer cells was maximized at the plasma jet treatment region, where the APPJ directly contacted the sample, and declined radially outward. As incubation continued, DNA damage in cancer cells decreased slightly over the first 4 h before rapidly decreasing by approximately 60% at 8 h post-treatment. In nonmalignant cells, no damage was observed within 1 h after treatment, but damage was detected 2 h after treatment. Notably, the damage was 5-fold less than that detected in irradiated cancer cells. Moreover, examining damage with respect to the cell cycle showed that S phase cells were more susceptible to DNA damage than either G1 or G2 phase cells. The proposed methodology for large-scale image analysis is not limited to APPJ post-treatment applications and can be utilized to evaluate biological samples affected by any type of radiation, and, more so, the cell-cycle classification can be used on any cell type with any nuclear DNA staining.

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