Inclusion of a blend of copaiba, cashew nut shell and castor oil in the protein-energy supplement for grazing beef cattle improves rumen fermentation, nutrient intake and fibre digestibility

2020 ◽  
Vol 60 (8) ◽  
pp. 1039
Author(s):  
R. W. Teobaldo ◽  
N. F. De Paula ◽  
J. T. Zervoudakis ◽  
M. A. Fonseca ◽  
L. S. Cabral ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Castor Oil ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
In Vitro Experiment ◽  
Cashew Nut ◽  
Protein Energy ◽  
Cashew Nut Shell

Context Essential oils are secondary plant compounds extracted from plants, with potential for the modulation of rumen fermentation. Aims Two experiments, namely one in vivo and another in vitro, were conducted to analyse the effects of a commercial blend of essential oils (EO; copaiba (Copaifera langsdorffii), cashew nut shell (Anacardium occidentale) and castor oil (Ricinus communis) and monensin as dietary feed additives in protein–energy supplements (PES) provided to grazing beef cattle, on ruminal fermentation, intake, total nutrient digestibility and protein dietary efficiency. Methods In the in vivo experiment, four entire Nellore bulls cannulated in the rumen (374 ± 15.66 kg; mean ± s.d.) were used in a 4 × 4 Latin-square design to evaluate the effects of EO concentration and monensin on voluntary intake, digestibility, and rumen and metabolic characteristics of grazing beef cattle provided with supplementation during the rainy season. Treatments were as follows: control (CON; PES without additives); monensin (MON; PES with inclusion of monensin at 20 mg/kg DM consumed); EO150 (PES with inclusion of EO at 150 mg/kg DM consumed); EO300 (PES with inclusion of EO at 300 mg/kg DM consumed). In the in vitro experiment, the effects EO150, EO300 and EO450, MON and CON on DM and neutral detergent-fibre (NDF) digestibility, and total gas production, were evaluated in four consecutive runs using a gas-production (GP) system. Key results In the in vivo experiment, DM intake, forage DM intake, crude protein intake and NDF intake were similar (P > 0.05) between EO150 and MON, but both were greater than those in EO300 and CON (P < 0.05). A lower EO concentration (EO150) increased (P < 0.05) NDF digestibility and improved nitrogen utilisation efficiency. In the in vitro experiment, the addition of MON and EO150 did not modify (P > 0.05) GP, DM and NDF digestibility compared with the control, but EO300 and EO450 decreased GP at 12 and 24 h and decreased DM and NDF digestibility at 48 h compared with the control, MON and EO150. Conclusions In vivo and in vitro results suggested that EO (copaiba oil, cashew nut shell and castor) at low doses (150 mg/kg DM) has the potential to improve ruminal fermentation in grazing beef cattle receiving supplements, but medium and high doses of EO can have adverse effects. Implications EO blends could be an alternative to MON for grazing beef cattle with access to supplements.

scholarly journals In vitro gas production kinetics and digestibility in ruminant diets with different levels of cashew nut shell liquid

2018 ◽  
Vol 39 (4) ◽  
pp. 1669
Author(s):  
Tatiana García Díaz ◽  
Antonio Ferriani Branco ◽  
Luís Carlos Vinhas Ítavo ◽  
Geraldo Tadeu dos Santos ◽  
Silvana Teixeira Carvalho ◽  
...  
Keyword(s):  
Gas Production ◽  
Ammoniacal Nitrogen ◽  
Ruminal Fermentation ◽  
Cashew Nut Shell Liquid ◽  
Ruminal Ph ◽  
Cashew Nut ◽  
Production Kinetics ◽  
Ruminant Diets ◽  
Cashew Nut Shell

The aim of this study was to evaluate the effect of increasing levels of cashew nut shell liquid (CNSL) in ruminant diets on in vitro dry matter digestibility (IVDMD), gas production kinetics, ruminal fermentation parameters, ammoniacal nitrogen concentration (NH3-N), and pH of the artificial rumen contents. The experimental design was completely randomized in a factorial 5 x 4 + 1 design, with five concentrate levels (200, 400, 600, 800, and 1,000 g kg-1 DM) and four CNSL levels (0, 0.3, 0.6, and 1.2 g kg-1 DM), as well as a control diet comprising only whole-plant corn silage, totaling 21 treatments. The inclusion of concentrate linearly increased IVDMD, while CNSL levels showed a quadratic effect, with the maximum estimated at 0.5 g kg-1 of CNSL. The total gas production, the disappearance of the substrate, the fraction of slow degradation (fraction VF2), and the respective degradation rate (fraction µ2) linearly increased with increasing levels of concentrate in the diet. Increasing concentrate levels resulted in a linear increase in the concentration of NH3-N and a reduction in the pH of the rumen liquid. Increasing CNSL levels decreased the concentration of NH3-N and increased the ruminal pH. The inclusion of 0.5 g CNSL kg-1 in the ruminant diets improved IVDMD, without altering the kinetic parameters of ruminal fermentation. The addition of CNSL to ruminant diets reduces ammoniacal nitrogen production and can avoid drastic reductions in ruminal pH, favoring better fermentation in the rumen.

scholarly journals Does partial replacement of corn with glycerin in beef cattle diets affect in vitro ruminal fermentation, gas production kinetic, and enteric greenhouse gas emissions?

PLoS ONE ◽  
2018 ◽  
Vol 13 (6) ◽  
pp. e0199577 ◽  
Author(s):  
Pedro Del Bianco Benedeti ◽  
Mozart Alves Fonseca ◽  
Teshome Shenkoru ◽  
Marcos Inácio Marcondes ◽  
Eduardo Marostegan de Paula ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Greenhouse Gas Emissions ◽  
Greenhouse Gas ◽  
Gas Production ◽  
Partial Replacement ◽  
Ruminal Fermentation ◽  
Gas Emissions

In vitro fermentability and methane production of some alternative forages in Australia

2016 ◽  
Vol 56 (3) ◽  
pp. 641 ◽  
Author(s):  
Z. Durmic ◽  
P. J. Moate ◽  
J. L. Jacobs ◽  
J. Vadhanabhuti ◽  
P. E. Vercoe
Keyword(s):  
Brassica Napus ◽  
Methane Production ◽  
Volatile Fatty Acids ◽  
Plantago Lanceolata ◽  
Ammonia Concentration ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Brassica Napus L

A study was conducted to examine in vitro ruminal fermentation profiles and methane production of some alternative forage species (n = 10) in Australia. Extent of fermentation was assessed using an in vitro batch fermentation system, where total gas production, methane production, and concentrations in ruminal fluid of volatile fatty acids (VFA) and ammonia were measured. Forages varied in their fermentability, with highest total gas, methane, VFA and ammonia production recorded from selected samples of Brassica napus L. cv. Winfred. Lowest methane production (i.e. 30% less than that formed by the highest-producing one) was observed in Plantago lanceolata L. cv. Tonic and Cichorium intybus L. cv. Choice. Selected plants, including P. lanceolata L. cv. Tonic, Brassica rapa L. cv. Marco, Brassica napus L. cv. Hunter had reduced acetate : propionate ratio and/or ammonia concentration, along with relatively low methane production compared with other species tested, while overall fermentation was not affected. It was concluded that selected novel forages have some advantageous fermentability profiles in the rumen and, in particular, inhibit methane production. However, before these can be recommended as valuable supplementary feedstuffs for ruminants in Australia, further studies are needed to confirm these effects over a range of samples, conditions and in vivo.

scholarly journals In Vitro Fermentation Characteristics and Methane Mitigation Responded to Flavonoid Extract Levels from Alternanthera sissoo and Dietary Ratios

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 109
Author(s):  
Sukruthai Sommai ◽  
Anusorn Cherdthong ◽  
Chanon Suntara ◽  
Sarong So ◽  
Metha Wanapat ◽  
...  
Keyword(s):  
Methane Production ◽  
Crude Protein ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Acid Detergent Fiber ◽  
In Vitro Fermentation

Two experiments were conducted under this study: Experiment 1 was to study production yield, chemical composition, and in vitro degradability of Brazilian spinach (Alternanthera sissoo; BS) leaf and leaf + leaf-stalk at various maturity ages of 15, 30, 45, and 60 days after plantation and regrowth and Experiment 2 was to evaluate the effect of flavonoid extract from BS leaf and leaf + leaf-stalk and dietary ratios on ruminal gas production, fermentation characteristics, and in vitro degradability. Experiment 1 showed that maturity ages after planting and regrowth increased, the yield significantly increased. Increasing maturity ages significantly (p < 0.05) increased neutral detergent fiber and acid detergent fiber content and decreased crude protein content, total flavonoid (TF) content, and degradability for both leaf and leaf + leaf-stalk. Maturity ages from 15 to 30 days after plantation and regrowth resulted (p < 0.05) the highest TF content and degradability for both leaf and leaf + leaf-stalk. Thus, BS leaf and leaf + leaf-stalk samples from 15 to 30 days of age were used for flavonoid extraction and used in the Experiment 2. Experiment 2 was conducted according to a 3 × 5 factorial experiment. Three roughage to concentrate (R:C) ratios at 50:50, 40:60, and 30:70 were used, and five levels of flavonoid extract (FE) at 0, 10, 20, 30, and 40 mg of substrate dry matter (DM) were supplemented. Experiment 2 showed that R:C ratio and FE had an interaction effect only on acetate to propionate ratio. Varying R:C ratios significantly increased (p < 0.05) in vitro DM degradability, total volatile fatty acids (VFA), and propionate (C3) concentration. FE supplementation linearly (p < 0.05) increased total VFA and C3 concentration and decreased methane production and protozoal population. This study could conclude that FE from BS could effectively modulate ruminal fermentation and decrease methane production. However, in vivo study needs to elucidate in order to validate the present results.

scholarly journals Babassu mesocarp meal for ewe lambs feeding: In vitro ruminal fermentation and in vivo apparent digestibility

2020 ◽  
Vol 43 ◽  
pp. e51056
Author(s):  
Luciano Fernandes Sousa ◽  
Jhone Tallison Lira de Sousa ◽  
Érica Beatriz Schultz ◽  
Gilberto de Lima Macedo Júnior
Keyword(s):  
Block Design ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Test Technique ◽  
Ewe Lambs ◽  
Randomized Design ◽  
Ruminant Diets ◽  
Four Blocks

The purpose of the current study was to evaluate the in vitro fermentation (IVRF) and apparent in vivo digestibility (AIVD) of diets formulated with four inclusion levels of babassu mesocarp meal (BMM) (0.0; 7.5; 15.0 and 22.5%) in ewe lambs. The IVRF test was performed through the "Hohenheim Gas Test" technique, following a randomized complete block design, with four blocks (rumen fluids from four different bovines). Gas production was measured at 3, 6, 9, 12, 24, 48, 72, and 96 hours after incubation. For the AIVD trial, 20 ewe lambs were used in a completely randomized design with five replicates. The animals were housed in metabolic cages during the digestibility test. Diets with BMM showed lower fermentation levels than those without BMM. The BMM reduced the gas production per incubation time and dry matter effective degradability (DMED), possibly due to its chemical characteristics, or even to the influence of other factors, such as physical properties. The BMM negatively influenced the AIVD of DM. Thus, it can be stated that, despite being a starch source, BMM reduces ruminal fermentation parameters when included in ruminant diets, negatively interfering with diet digestibility. Therefore, its use should be cautious.

scholarly journals PSXI-17 Influence of hops on in vitro ruminal fermentation of corn grain

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 408-408
Author(s):  
Yamicela Castillo-Castillo ◽  
Raul Solis ◽  
Armando A Quintana ◽  
Claudio Arzola ◽  
Ana Luisa Olivas-Palacios ◽  
...  
Keyword(s):  
Methane Production ◽  
Beef Cows ◽  
Gas Production ◽  
Co2 Production ◽  
Ruminal Fermentation ◽  
Ruminal Fluid ◽  
Fermentation Parameters ◽  
Corn Grain

Abstract An in vitro incubation was carried out to evaluate the potential of hops (Humulus lupulus) as an alternative to antibiotics for upgrading animal production. Whole pellets of hops (Variety Galena) were ground and incubated in a batch culture of ruminal fluid (2000 mg of ground corn grain + 10 mL of fresh rumen liquor). Ruminal fluid was collected from two beef cows through an esophageal tube. The hops were incubated by 24 h at levels of 0, 800, 1600 and 2400 µg/mL. Data were analyzed statistically by analysis of variance using PROC GLM of SAS. Hops addition linearly decreased (P &lt; 0.01) gas production (GP; 90.89, 61.73, 36.63 and 28.37 µmol/g respectively) and methane production (MP; 9.76, 1.70, 1.30 and 0.46 µmol/g respectively). The CO2 production linearly increased as levels of hops increased (P &lt; 0.02; 87.5, 88.4, 98.1 and 99.3 µmol/g respectively). The ammonia-N production was reduced in treatment 2 with respect to other treatments (P &lt; 0.03; 12.6, 9.2, 13.7 and 13.5 µmol/g). Effects on ruminal fermentation of corn grain were dose dependent of hops. Addition of hops in ruminant feeding may offer a means to decrease ruminal methane production. Further research is needed to test efficacy of hops on other in vivo rumen-fermentation parameters.

scholarly journals Manipulation of rumen fermentation by bioindustrial products of cashew nut shell (Anacardiumoccidentale) to reduce methane and enhance other products

2018 ◽  
Vol 23 (2) ◽  
pp. 61
Author(s):  
Andi Saenab ◽  
Wiryawan KG ◽  
Retnani Y ◽  
Elizabeth Wina
Keyword(s):  
Propionic Acid ◽  
Ammonia Concentration ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Feed Additive ◽  
Plant Materials ◽  
Cashew Nut ◽  
Cashew Nut Shell ◽  
Different Levels

One of the strategies to reduce methane produced by ruminants is by the application of feed additive from plant materials. One of the potential plants is cashew plant especially its shell. The cashew nut shell can be processed to become 3 bioindustrial products; ie biofat, biochar, biosmoke. The aim of this research was to evaluate the effectivity of different levels of biofat, biochar and biosmoke against methane and other fermented products.This experiment was arranged in block randomised design. The treatments were: Control (substrate=S), Biofat: S+0.25 µLmL<sup>-1</sup>, S+0.5 µLmL<sup>-1</sup>, S+0.75 µLmL<sup>-1</sup>; Biochar: S+0.1 mgmL<sup>-1</sup>, S+0.2 mgmL<sup>-1</sup>, S+0.3 mgmL<sup>-1</sup>; Biosmoke: S + 2.5 µLmL<sup>-1</sup>, S+5.0 µLmL<sup>-1</sup>, S + level7.5 µLmL<sup>-1</sup>. Each treatment was done in duplicates and the<em> in vitro</em> experiment was repeated 4 times. The measured variables were: total gas production, methane production, (dry matter, organic matter, NDF) degradability, ammonia concentration (NH<sub>3</sub>) and partial VFA concentration. The results showed that biofat, biochar and biosmoke reduced methane productionas much as  43.88%, 24.21%, 37.88%  at the highest level of inclusion, respectively. NH<sub>3</sub> slightly increased by biochar and biosmoke addition compared to control. Molar proportion of acetic acid decreased and propionic acid increased by addition of cashew nut shell bioindustrial products compared to control. Organik matter degradability decreased significantly with biofat and biosmoke addition. Different mechanisms of biofat, biochar and biosmoke were proposed in affecting rumen fermentation. It can be concluded that each bioindustrial product of cashew nut shell (biofat, biochar and biosmoke) can be utilised as feed additive to reduce methane and increase propionic acid in the rumen fermentation.

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