scholarly journals Mapping and QTL analysis of the barley population Sloop × Halcyon

2003 ◽  
Vol 54 (12) ◽  
pp. 1145 ◽  
Author(s):  
B. J. Read ◽  
H. Raman ◽  
G. McMichael ◽  
K. J. Chalmers ◽  
G. A. Ablett ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Major Gene ◽  
Doubled Haploid Population ◽  
Phenotypic Variance ◽  
Net Blotch ◽  
Hordeum Vulgare L ◽  
Haploid Population ◽  
Spring Type ◽  
Grain Colour ◽  
Emergence Response

A genetic linkage map of Hordeum vulgare L. 1280 cM in length, composed of 257 AFLP, RFLP, SNP, and microsatellite markers, has been constructed. The map was based on a doubled haploid population made from the cross Sloop (spring type) × Halcyon (winter type). The genetic map was used to identify qualitative major genes and quantitative trait loci (QTLs) affecting traits related to growth and flowering, grain colour, and disease resistance. Nine QTLs associated with grain colour (brightness, redness, yellowness, blue aleurone colour), plant height, 'intrinsic lateness', awn emergence, response to photoperiod, and spring or winter habit were located on 1H, 2H, 3H, 4H, and 5H. Eight QTLs associated with resistance to scald, net form of net blotch, leaf rust and powdery mildew were identified on chromosomes 1H, 2H, 3H, 4H, 5H, and 7H. The estimated magnitude of the QTL effects ranged from 9 to 85% of the total phenotypic variance. Resistances to leaf scald, net blotch, and leaf rust, and photoperiod and grain colour, were each controlled by at least one major gene.

scholarly journals Mapping Quantitative Trait Loci for 1000-Grain Weight in a Double Haploid Population of Common Wheat

2020 ◽  
Vol 21 (11) ◽  
pp. 3960 ◽  
Author(s):  
Tao Liu ◽  
Lijun Wu ◽  
Xiaolong Gan ◽  
Wenjie Chen ◽  
Baolong Liu ◽  
...  
Keyword(s):  
Quantitative Trait ◽  
Genetic Distances ◽  
Snp Markers ◽  
Grain Weight ◽  
High Yield ◽  
Doubled Haploid Population ◽  
Phenotypic Variance ◽  
Yield Trait ◽  
Haploid Population ◽  
Trait Locus

Thousand-grain weight (TGW) is a very important yield trait of crops. In the present study, we performed quantitative trait locus (QTL) analysis of TGW in a doubled haploid population obtained from a cross between the bread wheat cultivar “Superb” and the breeding line “M321” using the wheat 55-k single-nucleotide polymorphism (SNP) genotyping assay. A genetic map containing 15,001 SNP markers spanning 2209.64 cM was constructed, and 9 QTLs were mapped to chromosomes 1A, 2D, 4B, 4D, 5A, 5D, 6A, and 6D based on analyses conducted in six experimental environments during 2015–2017. The effects of the QTLs qTgw.nwipb-4DS and qTgw.nwipb-6AL were shown to be strong and stable in different environments, explaining 15.31–32.43% and 21.34–29.46% of the observed phenotypic variance, and they were mapped within genetic distances of 2.609 cM and 5.256 cM, respectively. These novel QTLs may be used in marker-assisted selection in wheat high-yield breeding.

Mapping of major spot-type and net-type net-blotch resistance genes in the Ethiopian barley line CI 9819

Genome ◽  
2006 ◽  
Vol 49 (12) ◽  
pp. 1564-1571 ◽  
Author(s):  
O.M. Manninen ◽  
M. Jalli ◽  
R. Kalendar ◽  
A. Schulman ◽  
O. Afanasenko ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Resistance Genes ◽  
Phenotypic Variation ◽  
Major Gene ◽  
Net Blotch ◽  
Hordeum Vulgare L ◽  
Gene Effects ◽  
Barley Line ◽  
Infection Response ◽  
Spot Type

Net blotch of barley ( Hordeum vulgare L.), caused by the fungal phytopathogen Pyrenophora teres Drechs. f. teres Smedeg., constitutes one of the most serious constraints to barley production worldwide. Two forms of the disease, the net form, caused by P. teres f. teres, and the spot form, caused by P. teres f. maculata, are differentiated by the type of symptoms on leaves. Several barley lines with major gene resistance to net blotch have been identified. Earlier, one of these was mapped in the Rolfi × CI 9819 cross to barley chromosome 6H, using a mixture of 4 Finnish isolates of P. teres f. teres. In this study, we used the same barley progeny to map resistance to 4 spot-type isolates and 4 net-type isolates of P. teres. With all net-type isolates, a major resistance gene was located on chromosome 6H, in the same position as described previously, explaining up to 88% of the phenotypic variation in infection response in the progeny. We designate this gene Rpt5. Several minor resistance genes were located on chromosomes 1H, 2H, 3H, 5H, and 7H. These minor genes were not genuinely isolate-specific, but their effect varied among isolates and experiments. When the spot-type isolates were used for infection, a major isolate-specific resistance gene was located on chromosome 5H, close to microsatellite marker HVLEU, explaining up to 84% of the phenotypic variation in infection response in the progeny. We designate this gene Rpt6. No minor gene effects were detected in spot-type isolates. The Ethiopian 2-rowed barley line CI 9819 thus carries at least 2 independent major genes for net-blotch resistance: Rpt5, active against net-type isolates; and Rpt6, active against specific spot-type isolates.

scholarly journals Identification and chromosomal location of major genes for resistance to Pyrenophora teres in a doubled-haploid barley population

Genome ◽  
2006 ◽  
Vol 49 (7) ◽  
pp. 855-859 ◽  
Author(s):  
T L Friesen ◽  
J D Faris ◽  
Z Lai ◽  
B J Steffenson
Keyword(s):  
Doubled Haploid ◽  
Doubled Haploid Population ◽  
Pyrenophora Teres ◽  
Net Blotch ◽  
Seedling Resistance ◽  
Drechslera Teres ◽  
Haploid Population ◽  
Barley Population ◽  
Spot Type ◽  
Simple Sequence

Net blotch, caused by Pyrenophora teres, is one of the most economically important diseases of barley worldwide. Here, we used a barley doubled-haploid population derived from the lines SM89010 and Q21861 to identify major quantitative trait loci (QTLs) associated with seedling resistance to P. teres f. teres (net-type net blotch (NTNB)) and P. teres f. maculata (spot-type net blotch (STNB)). A map consisting of simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers was used to identify chromosome locations of resistance loci. Major QTLs for NTNB and STNB resistance were located on chromosomes 6H and 4H, respectively. The 6H locus (NTNB) accounted for as much as 89% of the disease variation, whereas the 4H locus (STNB resistance) accounted for 64%. The markers closely linked to the resistance gene loci will be useful for marker-assisted selection.Key words: disease resistance, Drechslera teres, molecular markers.

Identification of novel QTLs for seedling and adult plant leaf rust resistance in a wheat doubled haploid population

2009 ◽  
Vol 119 (2) ◽  
pp. 263-269 ◽  
Author(s):  
C.-G. Chu ◽  
T. L. Friesen ◽  
S. S. Xu ◽  
J. D. Faris ◽  
J. A. Kolmer
Keyword(s):  
Leaf Rust ◽  
Doubled Haploid ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
Doubled Haploid Population ◽  
Plant Leaf ◽  
Haploid Population

scholarly journals Bivariate analysis of barley scald resistance with relative maturity reveals a new major QTL on chromosome 3H

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Xuechen Zhang ◽  
Ben Ovenden ◽  
Beverley A. Orchard ◽  
Meixue Zhou ◽  
Robert F. Park ◽  
...  
Keyword(s):  
Physical Map ◽  
Major Gene ◽  
Genetic Material ◽  
Seedling Stage ◽  
Bivariate Analysis ◽  
Doubled Haploid Population ◽  
Infected Leaf ◽  
Seedling Resistance ◽  
Haploid Population ◽  
Major Qtl

AbstractThe disease scald of barley is caused by the pathogen Rhynchosporium commune and can cause up to 30–40% yield loss in susceptible cultivars. In this study, the Australian barley cultivar ‘Yerong’ was demonstrated to have resistance that differed from Turk (Rrs1 (Rh3 type)) based on seedling tests with 11 R. commune isolates. A doubled haploid population with 177 lines derived from a cross between ‘Yerong’ and the susceptible Australian cultivar ‘Franklin’ was used to identify quantitative trait loci (QTL) for scald resistance. A QTL on chromosome 3H was identified with large effect, consistent with a major gene conferring scald resistance at the seedling stage. Under field conditions, a bivariate analysis was used to model scald percentage of infected leaf area and relative maturity, the residuals from the regression were used as our phenotype for QTL analysis. This analysis identified one major QTL on chromosome 3H, which mapped to the same position as the QTL at seedling stage. The identified QTL on 3H is proposed to be different from the Rrs1 on the basis of seedling resistance against different R. commune isolates and physical map position. This study increases the current understanding of scald resistance and identifies genetic material possessing QTLs useful for the marker-assisted selection of scald resistance in barley breeding programs.

QTL mapping of net blotch resistance genes in a doubled-haploid population of six-rowed barley

Euphytica ◽  
2004 ◽  
Vol 137 (3) ◽  
pp. 291-296 ◽  
Author(s):  
ZhengQiang Ma ◽  
Nora L.V. Lapitan ◽  
Brian Steffenson
Keyword(s):  
Qtl Mapping ◽  
Resistance Genes ◽  
Doubled Haploid ◽  
Doubled Haploid Population ◽  
Net Blotch ◽  
Haploid Population

scholarly journals Genetic Diversity and Population Structure of Serbian Barley (Hordeum vulgare L.) Collection during a 40-Year Long Breeding Period

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 118
Author(s):  
Ljiljana Brbaklić ◽  
Dragana Trkulja ◽  
Sanja Mikić ◽  
Milan Mirosavljević ◽  
Vojislava Momčilović ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Hordeum Vulgare ◽  
Crop Improvement ◽  
Genetic Erosion ◽  
Breeding Period ◽  
Phenotypic Variance ◽  
Hordeum Vulgare L ◽  
Phenotypic Data ◽  
Breeding Material

Determination of genetic diversity and population structure of breeding material is an important prerequisite for discovering novel and valuable alleles aimed at crop improvement. This study’s main objective was to characterize genetic diversity and population structure of a collection representing a 40-year long historical period of barley (Hordeum vulgare L.) breeding, using microsatellites, pedigree, and phenotypic data. The set of 90 barley genotypes was phenotyped during three growing seasons and genotyped with 338 polymorphic alleles. The indicators of genetic diversity showed differentiation changes throughout the breeding periods. The population structure discriminated the breeding material into three distinctive groups. The principal coordinate analysis grouped the genotypes according to their growth habit and row type. An analysis of phenotypic variance (ANOVA) showed that almost all investigated traits varied significantly between row types, seasons, and breeding periods. A positive effect on yield progress during the 40-year long breeding period could be partly attributed to breeding for shorter plants, which reduced lodging and thus provided higher yield stability. The breeding material revealed a considerable diversity level based on microsatellite and phenotypic data without a tendency of genetic erosion throughout the breeding history and implied dynamic changes in genetic backgrounds, providing a great gene pool suitable for further barley improvement.

Direct PCR-based genetic mapping of rice telomeric repeat associated sequences

Genome ◽  
1998 ◽  
Vol 41 (2) ◽  
pp. 193-198 ◽  
Author(s):  
Lishuang Shen ◽  
Lihuang Zhu
Keyword(s):  
Genetic Mapping ◽  
Oryza Sativa L ◽  
Telomeric Repeat ◽  
Doubled Haploid Population ◽  
Direct Pcr ◽  
Japonica Variety ◽  
Rapd Primer ◽  
Associated Sequences ◽  
Haploid Population ◽  
Pcr Method

Direct PCR-based genetic mapping of telomeric repeat associated sequences (TASs) was achieved using a RAPD primer mediated asymmetric PCR method. Twenty-two TAS loci were mapped in a rice doubled haploid population derived from a cross between an indica variety (Zhaiyeqing8) and a japonica variety (Jingxi17). Of these, 11 loci were mapped to the most distal position of seven chromosome arms and lengthened the linkage groups by 7.4-22.6 cM, five were mapped to the approximate positions of the centromeric regions, and six were mapped to other interstitial chromosomal regions.Key words: rice, Oryza sativa L., genetic mapping, telomeric repeat, telomeric repeat associated sequences, RAPD primer mediated PCR.

Kernel colour varies with cultivars and environments in barley

1998 ◽  
Vol 78 (2) ◽  
pp. 217-222 ◽  
Author(s):  
M. J. Edney ◽  
T. M. Choo ◽  
D. Kong ◽  
T. Ferguson ◽  
K. M. Ho ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Key Words ◽  
Western Canada ◽  
Net Blotch ◽  
Hordeum Vulgare L ◽  
Eastern Canada ◽  
Key Words Barley

Kernel colour is an important marketing trait for both malting and feed barleys. Therefore a study was initiated to investigate the kernel colour of 75 Canadian barley (Hordeum vulgare L.) cultivars at three locations (Charlottetown, Ottawa and Bentley) across Canada in 1991 and 1992. Kernel colour was measured by an Instrumar Colormet Spectrocolorimeter. Kernel colour was found to be brighter at the two locations in eastern Canada (Charlottetown and Ottawa) than at the location in western Canada (Bentley). Two-row cultivars on average were more discoloured than six-row cultivars; eastern two-row were more discoloured than western two-row. Covered barleys were less discoloured than hulless barleys in five of the six environments, but covered barleys at Bentley in 1992 were more discoloured than hulless barleys. Kernel discolouration appeared to be associated with susceptibility to net blotch for six-row cultivars. More studies are needed on kernel discolouration of barley. Key words: Barley, Hordeum vulgare, kernel colour

Sign in / Sign up

Export Citation Format

Share Document