scholarly journals A comparison of the excretion rate of endogenous purine derivatives in the urine of Bos indicus and Bos taurus steers

2006 ◽  
Vol 57 (2) ◽  
pp. 173 ◽  
Author(s):  
M. K. Bowen ◽  
D. P. Poppi ◽  
S. R. McLennan ◽  
V. J. Doogan

Estimates of microbial crude protein (MCP) production by ruminants, using a method based on the excretion of purine derivatives in urine, require an estimate of the excretion of endogenous purine derivatives (PD) by the animal. Current methods allocate a single value to all cattle. An experiment was carried out to compare the endogenous PD excretion in Bos taurus and high-content B. indicus (hereafter, B. indicus) cattle. Five Holstein–Friesian (B. taurus) and 5 Brahman (> 75% B. indicus) steers (mean liveweight 326 ± 3.0 kg) were used in a fasting study. Steers were fed a low-quality buffel grass (Cenchrus ciliaris; 59.4 g crude protein/kg dry matter) hay at estimated maintenance requirements for 19 days, after which hay intake was incrementally reduced for 2 days and the steers were fasted for 7 days. The excretion of PD in urine was measured daily for the last 6 days of the fasting period and the mean represented the daily endogenous PD excretion. Excretion of endogenous PD in the urine of B. indicus steers was less than half that of the B. taurus steers (190 µmol/kg W0.75.day v. 414 µmol/kg W0.75.day; combined s.e. 37.2 µmol/kg W0.75.day; P < 0.001). It was concluded that the use of a single value for endogenous PD excretion is inappropriate for use in MCP estimations and that subspecies-specific values would improve precision.

2016 ◽  
Vol 56 (10) ◽  
pp. 1643 ◽  
Author(s):  
L. F. Prados ◽  
S. C. Valadares Filho ◽  
S. A. Santos ◽  
D. Zanetti ◽  
A. N. Nunes ◽  
...  

Forty 3/4 Bos indicus × 1/4 Bos taurus bulls (initial bodyweight = 214 ± 4 kg; 11 ± 0.2 months) were used in the study. Four bulls were slaughtered as reference and the other 36 bulls were allowed ad libitum consumption of treatment diets following a completely randomised 3 × 3 factorial design. The design included three diet concentrations of calcium (Ca) and phosphorus (P): standard, 50% of standard, and 75% of standard and three periods of feedlot confinement: 8, 16, and 24 weeks with four bulls per treatment combination. The diets were isonitrogenous and consisted of 60 : 40 corn silage to concentrate. Dry matter intake, crude protein, ether extract, organic matter, non-fibrous carbohydrates, Ca, and P, expressed as kg/day, were greater (P < 0.05) for bulls confined for longer periods. Nutrient digestibility and average daily gain was not affected (P > 0.05) by Ca and P concentration of diet or by feedlot phase. Dressing were decreased (P < 0.05) for bulls that remained in confinement for a shorter period. The daily maintenance requirements of Ca and P were 13.66 and 21.51 mg/kg empty bodyweight, respectively, and the absorption coefficients of Ca and P were 0.70 and 0.67, respectively. We concluded that for crossbred cattle in the feedlot, dietary levels of Ca and P recommended by BR-CORTE (2010), NRC (2000), and AFRC (1991) could be reduced by 62%, 66%, and 57%, respectively, for Ca and by 14%, 15%, and 43%, respectively, for P with significant savings in costs. This study shows that Ca and P can be reduced to 1.8 and 2.2 g/kg DM respectively, in the diets for crossbred cattle in feedlot.


1985 ◽  
Vol 53 (3) ◽  
pp. 649-656 ◽  
Author(s):  
R. A. Hunter ◽  
B. D. Siebert

1. In a number of experiments voluntary food intake of three low-quality roughages, either alone or supplemented with rumen-degradable nitrogen and sulphur and minerals, was measured in Brahman (Bos indicus) and Hereford (Bos taurus) steers. The chaffed hays were Spear grass (Heteropogon conform) (6.2 g N/kg organic matter (OM)), Pangola grass (Digitaria decumbens) (7.9 g N/kg OM), and Pangola grass (12.0 g N/kg OM). Rumen characteristics relating to rate of fluid outflow from the rumen were also determined.2. There was no significant difference between breeds in the dry-matter intakes of the unsupplemented diets which ranged from 11.3 to 17.8 g/kg body-weight (BW) by Herefords and from 11.8 to 16.1 g/kg BW by Brahmans.3. Supplementation of Spear grass with N and S significantly (P < 0.05) increased intake by Herefords (24%) but not by Brahmans. When the lower-N Pangola grass was supplemented there was a significant increase in intake by both breeds with the magnitude of the response in Herefords (42%) (P < 0.001) being greater than that in Brahmans (15%) (P < 0.05). The intakes of both the supplemented Spear grass and the lower-N Pangola diets were significantly (P < 0.05) greater by Herefords than Brahmans. There was no breed difference in intake when the higher-N Pangola grass was supplemented. Both breeds recorded an 8% intake response to supplementation, although the increase was only significant (P < 0.05) in Herefords.4. The mean retention time of fluid in the rumen on the unsupplemented Pangola grass diet of lower N content was 12.7 h in Brahmans compared with 17.5 h in Herefords (P < 0.01). When the higher-N Pangola was fed, both alone and supplemented, the mean retention times were similar on both diets (10.5 and 9.9 h for Herefords; 9.5 and 8.1 h for Brahmans for unsupplemented and supplemented diets respectively).5. Plasma urea concentrations were higher in Brahmans than in Herefords on all diets. Rumen ammonia concentrations were significantly (P < 0.001) higher in Brahmans than Herefords when the lower-N Pangola grass diet was unsupplemented.6. The intakes and the variable intake responses to supplementation between breeds and diets are discussed in relation to a number of animal and dietary factors.


1998 ◽  
Vol 67 (1) ◽  
pp. 49-58 ◽  
Author(s):  
P. Grimaud ◽  
D. Richard ◽  
A. Kanwé ◽  
C. Durier ◽  
M. Doreau

AbstractThe effect of underfeeding and ref eeding on digestion was studied in Bos taurus and Bos indicus cows. Eight nonlactating cows, four B. taurus and four B. indicus (live weight 156 kg and 207 kg respectively) were first given a forage-based diet at a level above energy maintenance requirements for 4 weeks (3·65 and 4·66 kg dry matter (DM) per day respectively for B. taurus and B. indicus). They were then restricted at a low level of intake for 2 months (1·83 and 2·33 kg DM per day respectively for B. taurus and B. indicus) and finally refed at the first level for 2 months. Digestion measurements were made before the underfeeding period, at 3 and 8 weeks of underfeeding and at 3 and 8 weeks ofref eeding. Organic matter apparent digestibility decreased with underfeeding and increased with refeeding (0·637, 0·591, 0·652, 0·692 and 0·669 in B. taurus and 0·674, 0·560, 0·580, 0·698 and 0·692 in B. indicus, respectively 1 week before, 3 and 8 weeks after underfeeding, and 3 and 8 weeks after refeeding). This lower apparent digestibility at low level of intake was not expected either by ruminal particle retention time, which increased when intake decreased, or by measurements ofmicrobial activity: DM degradability measured in situ and ruminal particle size did not vary with level of intake. An effect of the length of underfeeding and refeeding was seen: the apparent digestibility tended to increase after several weeks of undernutrition and was higher after refeeding than before underfeeding. No difference was observed between the two genotypes.


1968 ◽  
Vol 10 (4) ◽  
pp. 373-380 ◽  
Author(s):  
A. Rogerson ◽  
H. P. Ledger ◽  
G. H. Freeman

1. The voluntary dry-matter and water intakes of indigenous and exotic cattle on high plane rations have been compared under similar conditions of management.2. At similar live weights, exotic cattle have a much higher feed intake relative to maintenance than indigenous cattle.3. Water intake was closely related to dry-matter intake, irrespective of live weight, and varied only very slightly between the two types of cattle.4. Exotic cattle were more than twice as efficient as indigenous cattle in converting feed into live-weight gain. It is suggested that this is largely a reflection of the increased feed intake, relative to maintenance requirements.5. Stage of maturity, degree of fatness and their relation to conversion efficiency are discussed.


2008 ◽  
Vol 48 (11) ◽  
pp. 1415 ◽  
Author(s):  
R. Watson ◽  
R. Polkinghorne ◽  
A. Gee ◽  
M. Porter ◽  
J. M. Thompson ◽  
...  

The effect of several different hormonal growth promotant (HGP) implant strategies on the palatability and carcass traits of different muscles in beef carcasses was investigated using samples from heifer and steer carcasses from a Bos indicus composite breed. In experiment 1, there were seven different implant strategies evaluated in heifers that were given different combinations of up to three implants (implanted at weaning, during backgrounding and at feedlot entry). A total of 112 heifers were slaughtered and 11 muscles or portions were collected from both sides [Mm. adductor femoris, gracilus, semimembranosus, longissimus dorsi lumborum, triceps brachii caput longum, semispinalis capitis, serratus ventralis cervicis, spinalis dorsi, biceps femoris (syn. gluteobiceps), tensor fasciae latae, gluteus medius (both the ‘D’ and the ‘eye’ portions) rectus femoris, vastus intermedius, vastus lateralis and vastus medialis]. These muscles were used to prepare a total of 1030 sensory samples which were aged for either 7 or 21 days and frozen. Thawed samples were cooked using different cooking methods (grill, roast and stir frying) before being evaluated by a consumer taste panel that scored samples for tenderness, juiciness, like flavour and overall liking. Experiment 2 used the steer portion from the same calving, which were treated to a similar array of HGP strategies, except that they were given up to four implants between weaning and slaughter at ~3 years of age. In experiment 2, there was a total of 12 different HGP implant strategies tested. At boning, three muscles (Mm. psoas major, longisimuss dorsi thoracis and lumborum portions) were collected from each of 79 carcasses with a total of 237 steak samples that consumers tested as grilled steaks. For both experiments, the mean of the HGP implant strategies resulted in increased ossification scores (P < 0.05) and decreased marbling scores (P < 0.05) compared with the controls, with the effect on ossification being much larger in the older steer groups. In both experiments, the different HGP strategies decreased (P < 0.05) all sensory scores compared with the controls, for all cooking method and muscle combinations. In experiment 1, there was no interaction between the mean HGP effect and muscle (P > 0.05), and aging rates differed among the muscles (P < 0.05). In experiment 2, there was a significant (P < 0.05) muscle × HGP treatment interaction, with a decrease in tenderness score due to HGP implant strategies in the M. longisimuss thoracis and lumborum portions, compared with no significant effect in the M. psoas major. For both experiments, there were no significant differences among the different implantation strategies on sensory scores (P > 0.05).


1955 ◽  
Vol 6 (5) ◽  
pp. 776 ◽  
Author(s):  
DF Dowling

The mean total skin thicknesses (mm) for the breeds sampled were: Devon 8.15, Hereford 6.7, Zebu cross 6.43, Australian Illawarra Shorthorn 6.23, Friesian 6.0, Zebu 5.77, Aberdeen Angus 5.75, Shorthorn 5.69, and Jersey 5.46. The early maturing Shorthorn (Bos taurus L.) and the Zebu (Bos indicus L.) differed significantly in the depth of the papillary and reticular layers, and in the relative thickness of these skin layers. The mean thickness for the papillary layer; was 0.98 mm in the Zebu, 1.40 mm in the Zebu cross, and 1.70 mm in the Shorthorn. Thus the larger, more active sweat glands of the Zebu are more superficial. The corresponding reticular layer averaged 4.45 mm in the Zebu, 4.5 mm in the Zebu cross, and 4.08 mm in the Shorthorn. The thickness of the papillary layer relative to the total skin thickness was 18.3, 23.7, and 29.3 per cent., for the Zebu, Zebu cross, and Shorthorn respectively. The Zebu and the Jersey have relatively thin skins. Therefore a thick skin is not essential for adaptability to a hot environment. Inherent differences in the function of the follicles and the glands of the papillary layer may be critical characteristics for heat tolerance.


1969 ◽  
Vol 49 (3) ◽  
pp. 383-388
Author(s):  
A. L. Hoogendoorn ◽  
C. M. Grieve

Chromic oxide (Cr2O3) in paper pellets was used as an indicator for digestibility studies with four Holstein-Friesian cows fed rations composed of different roughage to concentrate ratios. Cows A and B received one pellet, or 3.46 g of Cr2O3, whereas cows C and D received three pellets, or 10.38 g of Cr2O3, daily.Recovery of Cr2O3 in the feces varied from 84 to 103% for animals A and B and from 92 to 95% for animals C and D. The coefficients of apparent digestion of dry matter were lower (P < 0.01) when the Cr2O3 method was used instead of the conventional method. When samples were taken at 0500, 0900, 1300 and 1700 hours, the Cr2O3 method gave average estimates of digestibility for animal pairs A, B and C, D that were 94.2 and 97.5% of those obtained by the conventional method. Standard deviations for both the Cr2O3 and conventional methods were relatively high.Excretion patterns of Cr2O3 were not signicantly correlated with those of crude protein, phosphorus and calcium. The average concentrations of crude protein, phosphorus and calcium in compounded feces samples collected at 0500, 0900, 1300 and 1700 hours were 101.3, 100.6 and 96.7% of those in samples of the total excretions.The daily administration of 10.38 g of Cr2O3 at 0900 hours for eight days, and the collection of fecal grab samples at 0500, 0900, 1300 and 1700 hours daily during the last five days seemed satisfactory for estimating apparent digestibility of dry matter.


1994 ◽  
Vol 45 (4) ◽  
pp. 795 ◽  
Author(s):  
H Hearnshaw ◽  
PF Arthur ◽  
R Barlow ◽  
PJ Kohun ◽  
RE Darnell

Post-weaning growth and body condition, puberty and pelvic size of 197 heifers comprising straightbred Hereford (HxH) and Brahman (BxB), first-cross (BxH) and back-cross (HxBH and BxBH) heifers were evaluated. The heifers were born over a 3 year period, and grazed improved and semi-improved pastures following weaning at Grafton, New South Wales. Prior to weaning, heifers had been reared by dams on three pasture systems (high, medium and low quality pastures). Heifers from low quality pre-weaning pasture had higher (P < 0.05) post-weaning liveweight gain than those from high and medium quality pastures. BxH heifers gained 71 g/day more (P < 0.05) than the mean gain of their contemporaries of the other genotypes, whose gains were similar, from weaning to either 26 or to 30 months of age. Liveweight at all ages was influenced by genotype x pre-weaning pasture system interaction. At 30 months of age, BxH heifers from high and medium pre-weaning pastures were the heaviest. At the same age, but from low quality pre-weaning pasture, heifers with crossbred dams (HxBH and BxBH) were the heaviest. Wither height depended significantly (P < 0.05) on the proportion of Bos indicus genes, increasing from 113.8 cm in the HxH heifers to 124.4 cm in the BxB heifers at 30 months of age. BxH heifers had a higher (P < 0.05) body condition score than their contemporaries of the other genotypes, which were in similar condition, at 26 and 30 months of age. On average (across pre-weaning pasture system), 9% of BxB heifers had reached puberty by 22 months of age compared to 62, 95, 82 and 64% (s.e.= 9) for HxH, HxBH, BxH and BxBH heifers respectively. No significant genotype differences were obtained in the height, width and size of the pelvic opening of the heifers, measured just prior to the beginning of the mating season at 26 months of age.


1973 ◽  
Vol 13 (61) ◽  
pp. 117 ◽  
Author(s):  
JE Frisch

The mortality rates, weight changes and associated effects of a. draught in 1969 were observed in a herd of Shorthorn x Hereford (SH), Brahman x SH (BX) and Africander x SH (AX) crossbred cows and small herds of purebred Brahman and Africander cows at 'Belmont', Queensland. About half of the cows calved over a two month period from mid-October 1969. All the cows were weighed in February 1968, February and October 1969 and in January 1970 and the data from the crossbreds analysed by the method of least squares. The weights and weight changes of both the calving and non-calving groups followed a similar pattern though the weight changes of the non-calving group were of greater magnitude. The weights showed that the three crossbreds reached the same mature weight but at different rates. The SH cows reached mature weight least rapidly, had the highest mean weight loss during the drought and had the lowest mean gains after the season broke. Weight changes both during and after the drought were however dependent on lactational status in February 1969. The mean weight loss of cows that weaned a calf in 1969 (wet) was similar for all breeds. However, the mean loss of the dry SH cows was significantly greater than that of the dry BX and AX cows. Previously wet cows exhibited compensatory gains after calving but the gain of the wet SH cows was the lowest of the crossbred means. The mean birth weight of the SH calves was reduced below the long term mean by about 4 kg but birthweights of BX and AX calves were similar to those in other years. During the drought the purebred Brahmans lost the least and the purebred Africanders lost the most weight of all breeds. However, the nature of the data precludes any strict comparisons from being drawn. Mortality rates of breeding cows were recorded from 1960 to 1969. Rates were generally highest for the SH, particularly in the drought years of 1965 and 1969. Some reasons are advanced which may explain the differences in performance of the breed types.


2010 ◽  
Vol 22 (1) ◽  
pp. 248 ◽  
Author(s):  
J. H. F. Pontes ◽  
K. C. F. Silva ◽  
A. C. Basso ◽  
C. R. Ferreira ◽  
G. M. G. Santos ◽  
...  

In recent years, Brazil has become the leading country in the world for the number of embryos produced in vitro (Thibier M 2009 IETS Embryo Transfer Newsletter 22, 12-19). This is partly due to the large numbers of Bos indicus animals in Brazil, making up about 80% of the total cattle. The mean oocyte production per ultrasound-guided follicular aspiration from Bos indicus is higher than those for European breeds (Pontes JHF et al. 2009 Theriogenology 71, 690-697). In the present study, we analyzed 5407 ovum pick ups (OPU) and compared the average production of total (n = 90,086) and viable (n = 64,826) oocytes and the number of embryos produced in vitro from Gir (Bos taurus indicus), Holstein (Bos taurus taurus), 1/4 Holstein × 3/4 Gir, and 1/2 Holstein-Gir crossbreed cows. To obtain oocytes, OPU was repeated from 4 to 7 times (mean = 5.7 ± 2.4) in each donor cow aged from 3 to 7 years (mean = 5.0 ± 2.3) during a 12-mo period. COCs (n = 90,086) obtained were classified according to the presence of cumulus cells and the oocyte cytoplasm aspect (homogeneous or heterogeneous/fragmented). The viable oocytes (n = 64,826) were in vitro matured for 24 h at 38.8°C in an atmosphere of 5% CO2 in air. Since this was a commercial programm, frozen sexed semen (2 × 106 mL-1) from Gir (n = 8) or Holstein (n = 7) sires previously tested for high efficiency was used for IVF. Fertilization was carried out (18-20 h) and the presumed embryos were cultured for 7 days in the same conditions as were used for IVM. Data were analyzed by ANOVA. On average, 16.7 ± 6.2 oocytes were obtained per OPU/IVF procedure and 71.96% were considered viable. The mean numbers of total oocytes per OPU/IVF procedure were 17.1 ± 4.4 for Gir cows (n = 617), 11.4 ± 3.9 for Holstein cows (n = 180), 20.4 ± 5.8 for 1/4 Holstein × 3/4 Gir (n = 44), and 31.4 ± 5.6 for 1/2 Holstein-Gir crossbreed females (n = 37, P < 0.01). The mean numbers of viable oocytes per OPU/IVF procedure were 12.1 ± 3.8 for Gir cows, 8.0 ± 2.6 for Holstein cows, 16.8, ± 5.0 for 1/4 Holstein × 3/4 Gir, and 24.3 ± 4.7 for 1/2 Holstein-Gir crossbreed females (P < 0.01). The average number of embryos produced by OPU/IVF were 3.2 (n = 12,243/3378) for Gir cows, 2.2 (n = 2426/1138) for Holstein cows, 3.9 (n = 1033/267) for 1/4 Holstein × 3/4 Gir, and 5.5 (n = 1222/224) for 1/2 Holstein-Gir. The average number of embryos produced per IVF session from 1/2 taurus × indicus donor cows was greater (P < 0.01) than from Bos indicus cows. The number of recoverable and viable oocytes and the number of embryos produced in vitro from Bos indicus donors were higher than from Bos taurus females. Therefore, the highest oocyte yield and the greatest embryo production were obtained from 1/2 taurus × indicus females. This work was supported by In Vitro Brasil.


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