Variation in fatty acid composition among genetically homogeneous seeds of canola (Brassica napus), and implications for genotypic selection based on single seeds

2008 ◽  
Vol 59 (10) ◽  
pp. 926 ◽  
Author(s):  
M. N. Aslam ◽  
S. Kailis ◽  
M. N. Nelson ◽  
K. L. Bayliss ◽  
W. A. Cowling
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Brassica Napus ◽  
Sampling Variance ◽  
Oleic Acid Content ◽  
Brassica Napus L ◽  
Single Seed ◽  
Fa Composition ◽  
Single Seeds ◽  
Repeated Samples

Selection of canola (Brassica napus L.) for fatty acid (FA) composition is often based on single seeds or small seed lots, but information is lacking on variation among repeated samples. Variation in FA composition was measured among repeated samples of B. napus seeds by gas chromatography on oil extracted by cold solvents, from a selfed doubled-haploid line (Monty-028DH), and an open-pollinated B. napus cv. Surpass 501TT. The variation in FA composition among repeated samples of 1, 3, 5, and 10 whole seeds was compared with the equivalent weight of ground seeds or Soxhlet-extracted oil. The standard deviation of oleic acid for whole seeds of Monty-028DH decreased from 2.12% (single-seed samples) to 0.12% (groups of 10 seeds), but was constant across an equivalent range in weight of ground seed (0.15%) and in volume of Soxhlet-extracted oil (0.05%). A single-seed sample is not sufficient to separate two B. napus genotypes differing in mean oleic acid content of 60% and 70% at the 95% confidence interval, based on the sampling variance measured in these experiments. Similar trends were found for all the major FAs in both varieties.

scholarly journals Overexpression of Soybean Transcription Factors GmDof4 and GmDof11 Significantly Increase the Oleic Acid Content in Seed of Brassica napus L.

Agronomy ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 222 ◽  
Author(s):  
Qinfu Sun ◽  
Jueyi Xue ◽  
Li Lin ◽  
Dongxiao Liu ◽  
Jian Wu ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Transcription Factors ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Brassica Napus ◽  
Acid Content ◽  
Fatty Acid Biosynthesis ◽  
Oleic Acid Content ◽  
Acid Biosynthesis ◽  
Brassica Napus L

Rapeseed (Brassica napus L.) with substantial lipid and oleic acid content is of great interest to rapeseed breeders. Overexpression of Glycine max transcription factors Dof4 and Dof11 increased lipid accumulation in Arabidopsis and microalgae, in addition to modifying the quantity of certain fatty acid components. Here, we report the involvement of GmDof4 and GmDof11 in regulating fatty acid composition in rapeseeds. Overexpression of GmDof4 and GmDof11 in rapeseed increased oleic acid content and reduced linoleic acid and linolenic acid. Both qPCR and the yeast one-hybrid assay indicated that GmDof4 activated the expression of FAB2 by directly binding to the cis-DNA element on its promoters, while GmDof11 directly inhibited the expression of FAD2. Thus, GmDof4 and GmDof11 might modify the oleic acid content in rapeseed by directly regulating the genes that are associated with fatty acid biosynthesis.

scholarly journals Early Maturing Rapeseed (Brassica Napus L.) F1 Hybrid Cultivar 'Joan' with High Yield and Oleic Acid Content

2021 ◽  
Vol 53 (3) ◽  
pp. 305-310
Author(s):  
Kwang-Soo Kim ◽  
Yong-Hwa Lee ◽  
Ji-Eun Lee ◽  
Young-Lok Cha ◽  
Da-Hee An ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Brassica Napus ◽  
Acid Content ◽  
High Yield ◽  
Oleic Acid Content ◽  
Brassica Napus L ◽  
F1 Hybrid ◽  
Early Maturing ◽  
Hybrid Cultivar

scholarly journals Nitro-Oleic Acid in Seeds and Differently Developed Seedlings of Brassica napus L.

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 406 ◽  
Author(s):  
Martin Vollár ◽  
Gábor Feigl ◽  
Dóra Oláh ◽  
Attila Horváth ◽  
Árpád Molnár ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Brassica Napus ◽  
Oleic Acid Content ◽  
Brassica Napus L ◽  
No Donor ◽  
High Oleic ◽  
Signalling Molecules ◽  
First Time ◽  
Sensitive Electrode

Similar to animals, it has recently been proven that nitro-fatty acids such as nitro-linolenic acid and nitro-oleic acid (NO2-OA) have relevant physiological roles as signalling molecules also in plants. Although NO2-OA is of great therapeutic importance, its presence in plants as a free fatty acid has not been observed so far. Since Brassica napus (oilseed rape) is a crop with high oleic acid content, the abundance of NO2-OA in its tissues can be assumed. Therefore, we quantified NO2-OA in B. napus seeds and differently developed seedlings. In all samples, NO2-OA was detectable at nanomolar concentrations. The seeds showed the highest NO2-OA content, which decreased during germination. In contrast, nitric oxide (•NO) levels increased in the early stages of germination and seedling growth. Exogenous NO2-OA treatment (100 µM, 24 h) of Brassica seeds resulted in significantly increased •NO level and induced germination capacity compared to untreated seeds. The results of in vitro approaches (4-Amino-5-methylamino-2′,7′-difluorofluorescein (DAF-FM) fluorescence, •NO-sensitive electrode) supported the •NO liberating capacity of NO2-OA. We observed for the first time that Brassica seeds and seedlings contain free NO2-OA which may be involved in germination as an •NO donor as suggested both by the results of exogenous NO2-OA treatment of seeds and in vitro approaches. Due to their high NO2-OA content, Brassica sprouts can be considered as a good source of dietary NO2-OA intake.

scholarly journals TILLING-by-Sequencing+ Reveals the Role of Novel Fatty Acid Desaturases (GmFAD2-2s) in Increasing Soybean Seed Oleic Acid Content

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1245
Author(s):  
Naoufal Lakhssassi ◽  
Valéria Stefania Lopes-Caitar ◽  
Dounya Knizia ◽  
Mallory A. Cullen ◽  
Oussama Badad ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Content ◽  
Edible Oils ◽  
Fatty Acid Desaturase ◽  
Soybean Seed ◽  
Oleic Acid Content ◽  
Fatty Acid Production ◽  
Tilling By Sequencing

Soybean is the second largest source of oil worldwide. Developing soybean varieties with high levels of oleic acid is a primary goal of the soybean breeders and industry. Edible oils containing high level of oleic acid and low level of linoleic acid are considered with higher oxidative stability and can be used as a natural antioxidant in food stability. All developed high oleic acid soybeans carry two alleles; GmFAD2-1A and GmFAD2-1B. However, when planted in cold soil, a possible reduction in seed germination was reported when high seed oleic acid derived from GmFAD2-1 alleles were used. Besides the soybean fatty acid desaturase (GmFAD2-1) subfamily, the GmFAD2-2 subfamily is composed of five members, including GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E. Segmental duplication of GmFAD2-1A/GmFAD2-1B, GmFAD2-2A/GmFAD2-2C, GmFAD2-2A/GmFAD2-2D, and GmFAD2-2D/GmFAD2-2C have occurred about 10.65, 27.04, 100.81, and 106.55 Mya, respectively. Using TILLING-by-Sequencing+ technology, we successfully identified 12, 8, 10, 9, and 19 EMS mutants at the GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E genes, respectively. Functional analyses of newly identified mutants revealed unprecedented role of the five GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E members in controlling the seed oleic acid content. Most importantly, unlike GmFAD2-1 members, subcellular localization revealed that members of the GmFAD2-2 subfamily showed a cytoplasmic localization, which may suggest the presence of an alternative fatty acid desaturase pathway in soybean for converting oleic acid content without substantially altering the traditional plastidial/ER fatty acid production.

Variability of Seed Fatty Acid Composition to Growing Degree-Days in High Oleic Acid Sunflower Genotypes

Helia ◽  
2015 ◽  
Vol 38 (62) ◽  
Author(s):  
Claudio Ferfuia ◽  
Maurizio Turi ◽  
Gian Paolo Vannozzi
Keyword(s):  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Composition ◽  
Inbred Line ◽  
Acid Content ◽  
Growing Degree Days ◽  
Environment Interaction ◽  
Oleic Acid Content ◽  
Degree Days

AbstractHigh temperature enhances the oleic acid content in the oil of normal cultivars but conflicting results are reported on temperature effects on oleic acid content in HO cultivars: either no effect or an increase in oleic acid content with temperature. To investigate the effects of temperature on HO genotypes under natural field conditions, a three-year field trial was conducted using two sowing dates and three HO genotypes (two inbred lines and one hybrid). To compare our results with previous works, growing degree-days (GDD) were computed (base temperature=6°C). GDD accumulated during the “flowering – 25 days after flowering” period influenced fatty acid composition of seed. Oleic and linoleic acid contents were affected by accumulated GDD in two HO genotypes (one inbred line and the hybrid). There was an increase of about 3% in oleic acid content as response to more high GDD accumulated. Their content was not modified by GDD in the other inbred line. There was a genotype×environment interaction that we suppose depending on modifier genes. These genetic factors affected oleic acid content. This indicated the importance of breeding targeted to select hybrids with a stable oleic acid content and higher than 90%. Saturated fatty acids (palmitic and stearic) were also influenced by temperature, and there was genetic variability among genotypes.

scholarly journals Unconditional and conditional QTL analyses of seed fatty acid composition in Brassica napus L.

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Feng Chen ◽  
Wei Zhang ◽  
Kunjiang Yu ◽  
Lijie Sun ◽  
Jianqin Gao ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Brassica Napus ◽  
Acid Composition ◽  
Brassica Napus L ◽  
Conditional Qtl ◽  
Seed Fatty Acid
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