Wether bioassay of animal pasture legumes. II. The oestrogenic activity of nine strains of Trifolium subterraneum L.

1964 ◽  
Vol 15 (4) ◽  
pp. 527 ◽  
Author(s):  
AJ Millington ◽  
CM Francis ◽  
NR McKeown

Nine strains of subterranean clover (Trifolium subterraneum L.) were assayed for oestrogenic activity with changes in wether teat length as the criterion. The isoflavone and coumestrol contents of each strain were determined by thin-layer chromatography. Significant differences were obtained between strains. Multiple regression analysis indicated a positive relationship between increase in teat length and the formononetin content of the strains. No such relationship was found for either genistein or biochanin A, the other isoflavones that were present in appreciable amounts.

Author(s):  
Dra. Rr. Sri Diniarti, S.E.

This research is an explanatory research, because it explaining about the relationship among the hypnotized variables. This research aims to knowing about the relationship and the influence between the accounts, location to the other bank, and loans to the net margin in Bank Jatim Madiun. The results are between accounts to net margin has positive relationship, between location to another bank to net margin has negative relationship, and between loans to net margin has positive relationship. Together, the variables have strong enough relationship to net margin, it showing by coefficient of determination value is 0,956, it means that the value close to 1. So, 4,4% of Profitability Net Margin influenced by the others variables.The method of research was by using field research that including observation, interview and library research.The analyzing data method was by using Multiple Regression Analysis, Correlation Analysis, and Coefficient of Determination Analysis.


1964 ◽  
Vol 15 (2) ◽  
pp. 223 ◽  
Author(s):  
AB Beck

A number of strains of subterranean clover has been examined for isoflavone content in an attempt to explain the differences of oestrogenic potency in sheep and cattle under field conditions. A method of thin-layer chromatography has been developed which allows a rapid semiquantitative estimation of the main isoflavones present in this species. The isoflavones are present in a combined form, probably as glycosides, and the free isoflavones are rapidly released after crushing the leaf. The percentages of formononetin, genistein, and biochanin A in the leaves are very much higher than reported by earlier workers and vary widely between strains. Moreover, the ratios of the isoflavones differ greatly among strains. The relevance of these plant data to the oestrogenic potency in the animal is discussed.


2003 ◽  
Vol 54 (2) ◽  
pp. 203 ◽  
Author(s):  
B. S. Dear ◽  
G. A. Sandral ◽  
D. Spencer ◽  
M. R. I. Khan ◽  
T. J. V. Higgins

A bxn gene, coding for the nitilase enzyme that catalyses the detoxification of the herbicide bromoxynil, was inserted into subterranean clover (Trifolium subterraneum L. subsp. yanninicum). The agronomic characteristics of 3 transgenic lines (BXN 5, 7, 10) and their response to herbicides containing bromoxynil, were compared with that of the non-transgenic parent, cv. Gosse, in 1 glasshouse and 2 field experiments.The application of bromoxynil at 1.5 L/ha resulted in a 50% reduction in the spring herbage yield of the non-transgenic control but no reduction in BXN 5 and 7. The level of the phyto-oestrogens, genistein and biochanin A, in the leaf tissue were low in Gosse and the 2 transgenic lines, BXN 7 and 10, but the level of genistein increased significantly (P < 0.05) from 0.85% in Gosse to 1.43% dry weight in BXN 5, and biochanin A increased from 0.35% in Gosse to 0.73% in BXN 5. The application of the herbicides bromoxynil, bromoxynil+MCPA, and Jaguar (bromoxynil+diflufenican) at the cotyledon stage in a glasshouse study significantly reduced the leaf area of the non-transgenic Gosse, but did not reduce the leaf area of the 3 transgenic lines. When applied at the 4–5 leaf stage, the leaf area of Gosse was again reduced by all the herbicides, but only the bromoxynil+MCPA treatment reduced the leaf area of the transgenic lines relative to the control. The application of bromoxynil or Jaguar at the 4–5 leaf stage depressed the herbage yield of Gosse by about 62% compared with 0–24% in the 3 transgenic lines. In the field studies, BXN 5 and 10 had similar seed yields to Gosse in the absence of herbicide application, but the seed yield of BXN 7 was significantly less than the other 2 transgenic lines. The level of hard-seed in BXN 7 was also lower at 25% compared with 55–57% in the other transgenic lines and Gosse.These findings stress the need to carefully assess the agronomic qualities of transgenic lines prior to release as although tolerance to bromoxynil may be greatly increased by the bxn gene, other important agronomic characteristics, such as seed yield, hardseededness, and isoflavone content, may change as result of the gene or transformation process.


1975 ◽  
Vol 15 (75) ◽  
pp. 541 ◽  
Author(s):  
DL Chatel ◽  
CM Francis

The resistance of 307 varieties of subterranean clover (Trifolium subterraneum) to the clover scorch disease pathogen was assessed in three locations in south west Australia. Disease intensity as measured by visual ratings of sward damage was found to vary with variety and location. Resistance was independent of subspecies groupings but dependent on country of origin. Spanish and Portugese varieties were generally less susceptible than North African selections. Disease severity was also related to plant characters by correlation and multiple regression analysis. Late maturing varieties were most resistant under the test conditions and rapidly growing earlier varieties, with large pale leaves and thick petioles, were least resistant.


2017 ◽  
Vol 11 (1) ◽  
pp. 115
Author(s):  
Sri Diniarti

This research is an explanatory research, because it explaining about the relationship among the hypnotized variables. This research aims to knowing about the relationship and the influence between the accounts, location to the other bank, and loans to the net margin in Bank Jatim Madiun. The results are between accounts to net margin has positive relationship, between location to another bank to net margin has negative relationship, and between loans to net margin has positive relationship. Together, the variables have strong enough relationship to net margin, it showing by coefficient of determination value is 0,956, it means that the value close to 1. So, 4,4% of Profitability Net Margin influenced by the others variables.The method of research was by using field research that including observation, interview and library research.The analyzing data method was by using Multiple Regression Analysis, Correlation Analysis, and Coefficient of Determination Analysis.


2016 ◽  
Vol 1 (1) ◽  
pp. 1-15
Author(s):  
Frederich Oscar Lontoh

This research is titled " The influence of sermon, church music and church facilities on the level of attendance”. The purpose of research is to identify and analyze whether sermon, church music and church facilities have influence on the the level of attendance. The target population in this study is a Christian church members who live in the city of Surabaya.. Sample required is equal to 47 respondents. Through sampling stratified Random techniques.These influence was measured using Pearson correlation coefficient and multiple regression analysis, t-test and analysis of variance. Descriptive  analysis  were taken to analyze the level of attendance according to demographic groups.The hypothesis in this study are the sermon, church music and church facilities have positive and significant on the level of attendance. The results showed that collectively, there are positive and significant correlation among the sermon, church music and church facilities on the level of attendance  96,2%. It means that 96,2 % of level of attendance influenced by sermon, church music and church facilities and the other 28,9% by others. All of the variable partially have significant correlation to level of attendance.


2019 ◽  
Vol 26 (6) ◽  
pp. 1001-1020
Author(s):  
Jackson de Souza ◽  
Luiz Mendes-Filho ◽  
Dimitrios Buhalis

This study focuses on the evaluation of the tourist destination advertising effectiveness. The destination advertising response DAR model was used to analyze data on the effectiveness of destination promotional campaigns on visitor expenditure, in six trip facets: destination, accommodations, attractions, restaurants, events, and shopping. Independent sample t-tests were conducted to identify any differences in total destination spending among the groups of those visitors influenced for each trip facet. A multiple regression analysis was performed to discriminate the performance of the travel facets expenditures in the estimation of total expenditures. Significant results indicate that the “destination,” “accommodations,” and “restaurants” facets directly influence the total expenditures. Self-planners had the highest variance, explaining in total visitor expenditure compared to the regression analysis results of the other two groups (i.e. travel agencies and online travel agencies). The study also explores how destinations can improve their competitiveness on tourist advertising by using technologies.


1969 ◽  
Vol 20 (6) ◽  
pp. 1043 ◽  
Author(s):  
RC Rossiter

In experiments with young plants of the Dwalganup strain of subterranean clover, nitrogen deficiency was associated with increased concentrations of isoflavones in the expanded leaves and cotyledons. In the first trifoliate leaves the concentration of total isoflavones (formononetin+genistein+ biochanin A) was approximately doubled at low nitrogen supply. Biochanin A was much less affected than the other two isoflavones. The increase in isoflavone contents of nitrogen-deficient leaves was associated with decreased protein synthesis, but not always with increased sugar contents. The supply of carbon substrates for isoflavone synthesis may depend on starch as well as sugar contents. Practical implications of the results are considered briefly.


1965 ◽  
Vol 16 (6) ◽  
pp. 915 ◽  
Author(s):  
JR Simpson

The extent of underground transference of nitrogen from three pasture legumes, each growing in association with a grass, has been studied in pot culture under several systems of management during the first 12–18 months after sowing. The three legumes performed quite differently. Subterranean clover did not release any nitrogen until senescence and then produced a rapid transference. White clover was competitive for nitrogen until the autumn-winter period. Lucerne released nitrogen gradually over the whole experimental period. Frequent defoliation of the legumes reduced competition for nitrogen by white clover but also reduced transference from the other legumes. Killing the perennial legumes produced only a small temporary increase in transference. Wilting and temporary drying treatments also reduced the transference. Thus there was no evidence that the nitrogen transference from lucerne was due to a shedding or decay of nodules induced by defoliation; it could equally well be due to direct excretion of nitrogen from the intact root system. The significance of the results in pasture establishment in infertile areas is discussed.


1955 ◽  
Vol 6 (2) ◽  
pp. 211 ◽  
Author(s):  
Y Aitken

The value of the annual legume Trifolium subterraneum L. (subterranean clover) in Australian agriculture warrants more precise knowledge of factors affecting flowering and prolific seeding. The effect of temperature and photoperiod on flower initiation in early and later flowering varieties has been investigated in an effort to determine the geographical limits of the use of subterranean clover in Australia. At any time of sowing, the length of the growing season of a variety depends greatly on the variety's response to the temperature level and to the photoperiod of the first few weeks after germination. In all varieties of subterranean clover so far examined flower initiation is accelerated by a period of low temperature. In the later varieties, flower initiation is prevented by an insufficient period of low temperature. The length of the necessary cold period is shortened under longer photoperiod. Early varieties are early flowering because they do not require so long a cold period or so low a temperature as late varieties.


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