Change with length of feeding period in the oestrogenic response to ovariectomized ewes to ingested coumestans

1975 ◽  
Vol 26 (2) ◽  
pp. 305 ◽  
Author(s):  
RW Kelly ◽  
DR Lindsay

Three experiments were conducted to test the effect of the period of intake of feed containing coumestans, and the amount of coumestans in the feed, on the production of cervical mucus in ovariectomized ewes. After long periods of feeding of medic cubes, ewes produced much less mucus than after shorter periods of feeding when feed containing 525 ppm coumestrol and 935 ppm 4'-methoxycoumestrol was ingested. When feed containing approximately one-third of these amounts of coumestans was ingested, the production of cervical mucus initially increased, being greater after 4 than 1 day's exposure to the feed. After 8–16 days of feeding on these diets, ewes produced the same amount of mucus as those fed on a non-oestrogenic diet. The decline in the cervical mucus response suggested either that a deactivation mechanism for coumestans develops in the ewe, or that the ewe becomes refractory to coumestans.

1942 ◽  
Vol 1 (4) ◽  
pp. 285-292 ◽  
Author(s):  
Bradford Knapp ◽  
Ralph W. Phillips ◽  
W. H. Black ◽  
R. T. Clark

1956 ◽  
Vol 15 (3) ◽  
pp. 846-853 ◽  
Author(s):  
L. E. Lloyd ◽  
H. E. Peckham ◽  
E. W. Crampton
Keyword(s):  

2011 ◽  
Vol 31 (12) ◽  
pp. 1489-1495 ◽  
Author(s):  
Jiying WEI ◽  
Guifang FAN ◽  
Feng JIANG ◽  
Zhenzhong ZHANG ◽  
Lan ZHANG

2020 ◽  
Vol 220 ◽  
pp. 106361
Author(s):  
Laura Abril-Parreño ◽  
Jack Morgan ◽  
Anette Krogenæs ◽  
Xavier Druart ◽  
Mary E. Gallagher ◽  
...  
Keyword(s):  

2020 ◽  
Vol 98 (12) ◽  
Author(s):  
Hung Phuc Nguyen ◽  
Thinh Van Do ◽  
Hau Duc Tran

Abstract A 16-wk growth trial was conducted to examine the effects of dietary replacement of fish meal by defatted soybean meal (SBM) and fermented soybean meal (FSBM) with taurine supplementation on growth performance, nutrient apparent digestibility coefficient (ADC) and biological parameters of pompano fish. The FSBM was produced by fermenting SBM with Lactobacillus spp. Seven isonitrogenous and isoenergetic diets were formulated to replace 35% or 50% of fish meal by SBM or FSBM with taurine supplementation. The diets are denoted as follows: FM, SBM35, SBM35T, FSBM35T, SBM50, SBM50T, and FSBM50T. The FM (the basal diet) contained fish meal as a main source of dietary protein. Taurine was supplemented to SBM35T, FSBM35T, SBM50T, and FSBM50T at the level of 15 g/kg diet. Pompano juveniles with an initial body weight (BW) of 80 g reared in floating net cages were fed the experimental diets twice daily for 16 wk. Results showed that the final BW, weight gain, and feed conversion ratio of fish fed SBM35 and SBM50 were significantly lower than those of fish fed FM (P < 0.05), indicating that the replacement of fish meal by SBM at the rate of 35% in the diet is excessive for pompano. Supplementation of taurine to the SBM-included diets significantly increased growth performance and feed utilization (P < 0.05); however, these diets did not restore the performance back to a level equivalent to that of fish offered the basal diet. Meanwhile, fish fed FSBM35T had comparable growth and feed performances to those fed FM. Hematocrit values, total biliary bile acid levels, whole body lipid contents, and tissue taurine concentrations of fish fed SBM35 and SBM50 were the lowest among the treatments, but these parameters were improved by taurine supplementation and FSBM inclusion in the diet. Taurine supplementation increased lipid ADC, and SBM fermentation slightly enhanced both lipid and protein ADCs of the fish. These findings suggest that the combination of FSBM and taurine supplementation is an effective way to improve growth performance, nutrient digestibility, and biological parameters, and that FSBM with taurine supplementation can replace 35% of fish meal in pompano diets without any negative effects on growth and feed performances in a long-term feeding period.


2021 ◽  
Vol 611 ◽  
pp. 117976
Author(s):  
Aurélien Bonnin ◽  
Yannick Pouilloux ◽  
Vincent Coupard ◽  
Denis Uzio ◽  
Ludovic Pinard

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1582
Author(s):  
Jihwan Lee ◽  
Suhyun Lee ◽  
Younbae Park ◽  
Seokhyun Lee ◽  
Seungmin Ha ◽  
...  

To improve reproductive performance in cattle, the accurate detection of estrus and optimization of insemination relative to ovulation are necessary. However, poor heat detection by farm staff leads to a decreased conception rate, thus inflicting economic damage to the beef and dairy industries. This study aimed to develop monoclonal antibodies (mAb) that can specifically bind to the bovine lactoferrin (bLF) protein, which we have previously demonstrated to be overexpressed in bovine cervical mucus during estrus. Female rats were intraperitoneally immunized with bLF protein as the antigen. Anti-bLF mAbs were then purified by affinity chromatography, and their binding affinity for the bLF antigen was examined using ELISA. We found a high binding affinity between mAbs and bLF. Finally, we developed a rapid bovine heat detection kit using the anti-bLF mAbs that we generated and tested on cervical mucus from 12 cows (estrous synchronization, n = 2; natural cycling, n = 10). We found that the kits accurately detected estrus. Overall, our fabricated heat detection kit based on rat anti-bLF mAbs could pave the way for the development of potent tools for heat detection devices for dairy cattle, thereby preventing economic loss.


2021 ◽  
Vol 23 (14) ◽  
pp. 8916-8925
Author(s):  
Leila Shahrokh ◽  
Reza Omidyan ◽  
Gholamhassan Azimi

Excited-state deactivation mechanism of protonated cytosine and thymine is investigated based on ab initio and NAMD simulation methods. The ring deformation from C6 region is suggested to play the most prominent role in deactivation mechanism.


2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Yasaman Zia ◽  
Jennifer Velloza ◽  
Lynda Oluoch ◽  
Richard Momanyi ◽  
Sarah Mbugua ◽  
...  

Abstract Background For couples affected by HIV, and serodifferent couples in particular, pregnancy desire is often juxtaposed against the risk of HIV transmission between the couple and the potential neonate leading to thinking about measures to minimize risk of HIV transmission. We assess the use of fertility awareness methods [FAM] and evaluate the drivers of alignment between indicators of fertility and sexual behavior among HIV-serodifferent couples desiring pregnancy. Methods HIV-serodifferent couples from Thika, Kenya were enrolled into an open-label pilot evaluation of safer conception strategies. Women responded to daily 7-item short message service [SMS] surveys on FAM and sexual activity. Menstrual cycles were categorized as having condomless sex aligned, not aligned, or partially aligned to the predicted peak fertility. We used binomial logit models with generalized estimating equations to assess alignment between condomless sex during peak fertility days and FAM results. We used Cox proportional hazards to compare pregnancy incidence among months with sex and peak fertility aligned and mis-aligned. Results A total of 6929 SMS surveys across 252 menstrual cycles of 65 women were included. Reporting “sticky” cervical mucus (adjusted odds ratio [aOR]: 2.25, 95% confidence interval [95% CI]: 1.30, 3.90) and positive ovulation prediction kit [OPK] result (aOR: 2.07, 95% CI: 1.11, 3.86) were associated with increased likelihood of alignment of condomless sex during peak fertility. Pregnancy incidence was statistically similar among periods with sex aligned and not aligned with peak fertility. Conclusions Among women engaged in a comprehensive safer conception program, a moderate percentage of women aligned condomless sex and predicted peak fertility days at least once. While FAM, particularly cervical mucus and OPK, are an inexpensive option for couples to consider using as a component of their safer conception strategies, antiretroviral-based strategies remain important to minimize risk.


1978 ◽  
Vol 235 (1) ◽  
pp. R29-R34 ◽  
Author(s):  
P. R. McHugh ◽  
T. H. Moran

In seven male monkeys, Macaca mulatta, the infusion of nutrients into the stomach just prior to or 20 h before a 4-h feeding period reduced the feeding by an amount comparable to the calories infused. Pure carbohydrates, fat, protein, and mixtures were employed as infusions and given in a random fashion over a caloric range of 75-300 kcal. In a second series of experiments, monkeys were partially fasted on 1 day and in this way deprived of 75, 150, 300, or 450 kcal. On successive days, they overate to compensate for this deprivation. The smaller deprivations (75 and 150 kcal) were corrected on the first recovery day. The 300-kcal deprivation required 2 days to be corrected while the 450-kcal deficit was only partially restored. These experiments demonstrate the capacities of the monkey to respond with precision to caloric supply and deprivation so as to maintain a constant caloric intake.


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