Effect of calcium, pH and nitrogen on the growth and chemical composition of some tropical and temperate pasture legumes. I. Nodulation and growth

1976 ◽  
Vol 27 (5) ◽  
pp. 611 ◽  
Author(s):  
CS Andrew
Keyword(s):  
Dry Matter ◽  
Calcium Sulphate ◽  
Low Ph ◽  
Root Weight ◽  
Calcium Treatment ◽  
Pasture Legumes ◽  
Low Calcium ◽  
High Calcium ◽  
Effects Of Ph ◽  
Ph Range

The effects of a factorial combination of calcium (0.125, 2.0 mM calcium sulphate), pH (4.0, 4.5, 5.0, 6.0) and nitrogen (nil, 2.0mM ammonium nitrate) on the nodulation and growth of some tropical and temperate pasture legumes were determined. Macroptilium lathyroides and Lotononis bainesii were unaffected by low pH and low calcium (100% of plants nodulated at all treatments). In Stylosanthes humilis, Trifoliurn semipilosum, and T. rueppellianum reduced nodulation occurred only at pH 4.0. Desmodium uncinatum and Trifolium repens were sensitive to low pH, and nodulation was markedly reduced at pH 4.0 and below 5.0 respectively. Medicago sativa, M. truncatula, M. scutellata, and Glycine wightii were extremely sensitive, maximum nodulation occurring only at pH 6-0 and 2 . 0 mM calcium. Furthermore, the dry matter yields of plants that nodulated under inferior treatments were less than those of plants nodulated under optimum treatments. Nodulation and growth were strongly controlled by pH, and although interactions occurred with calcium treatment, these were primarily in the intermediate pH range. Dry matter yields of nodulated plants of S. humilis and L. bainesii were greater at the low calcium sulphate treatment (0.125mM) than at thc high treatment (2.0mM). The remaining legumes gave varying positive responses to the high calcium treatment. Root weight ratios of nodulated plants were decreased by the higher treatments, the minimum occurring at those treatments producing the highest dry matter. In the presence of applied nitrogen (no nodulation), the effects of pH and calcium on dry matter were small, but the trends were similar to those for nodulation and dry matter production of the nodulated plants in the Rhizobium series. Root weight ratios of plants in the nitrogen series were equal to or less than those for the maximum-yielding plants in the nodulation series. It is considered that both nodulation and subsequent plant growth are dominated by the hydrogen ion effect, and that the beneficial effect of calcium operates within certain pH limits. pH had little effect on plants that were well supplied with nitrogen.

Effect of calcium, pH and nitrogen on the growth and chemical composition of some tropical and temperate pasture legumes. II.* Chemical composition (calcium, nitrogen, potassium, magnesium, sodium and phosphorus)

1976 ◽  
Vol 27 (5) ◽  
pp. 625 ◽  
Author(s):  
CS Andrew ◽  
AD Johnson
Keyword(s):  
Chemical Composition ◽  
Dry Matter ◽  
Calcium Concentration ◽  
Dry Matter Production ◽  
Magnesium Concentration ◽  
Sand Culture ◽  
Calcium Treatment ◽  
Pasture Legumes ◽  
Low Calcium ◽  
Matter Production

The chemical composition of tops and roots of nodulated and non-nodulated plants of some tropical and temperate pasture legumes grown in sand culture is reported and discussed in relation to nodulation efficiency and dry matter production, and nutrient interaction. In most species, increasing pH raised the nitrogen concentrations in the tops of nodulated plants; calcium treatment had little effect. Increasing the calcium concentration of the nutrient solution from 0.125 to 2.0 mM markedly increased the calcium~ concentration in the tops of all species, but in the roots to only a minor extent. Concentrations in tops differed between species. Concentrations in the tops of modulated plants were increased as a result of increasing pH, particularly at the low calcium treatment and for those species sensitive to low pH (Glycine wightii, Medicago species). Increasing the calcium concentration lowered the magnesium concentration, but the total cation content was not significantly affected. In the tropical species, phosphorus concentrations in the tops were increased by reducing pH at both calcium treatments; values were higher at the low calcium treatment than at the high calcium treatment. Roots accumulated inore phosphorus than the tops. Differences in the response of the species cannot be directly related to their mineral composition. With respect to nodulation and dry matter production, it is considered that the pH is the dominant factor and operates in the nodule initiation phase and in subsequent nitrogen production. _______________ *Part I, Aust. J. Agric. Res., 27: 611 (1976).

The effect of solution pH and external calcium concentration on the early growth and nodulation of several annual Medicago species

1990 ◽  
Vol 41 (5) ◽  
pp. 933 ◽  
Author(s):  
MA Ewing ◽  
AD Robson
Keyword(s):  
Calcium Concentration ◽  
Acid Soils ◽  
Early Growth ◽  
Low Ph ◽  
Solution Ph ◽  
Nodule Number ◽  
Low Calcium ◽  
Wide Range ◽  
Effects Of Ph ◽  
Annual Medics

The study tested the hypothesis that annual medic species which nodulate well in acid soils in the field (M. murex Willd. and M. polymorpha L.) will nodulate better in acid solutions with low calcium concentrations than annual medics which nodulate poorly in acid soils (M, truncatula Gaertn.). Effects of pH (5.5 and 6.5) and calcium concentration (0.2, 0.5, 1 and 2 mM) on the early growth and nodulation of three annual medic species (M. truncatula, M. polymorpha and M. murex) were investigated. Increasing pH or calcium concentration did not increase plant growth for any of the species. However, nodulation was generally depressed by low pH for all species. Increasing calcium concentration in solution increased nodulation in all species. Effects of low pH and low calcium concentration in decreasing nodule number were much greater for M. truncatula than for M. polymorpha and M. murex. At pH 5.5, M. truncatula failed to nodulate at any calcium concentration, whereas a large proportion of M. murex plants nodulated at 1 mM calcium and some M. polymorpha plants nodulated at 2 mM calcium. At pH 6.5, M. polymorpha required 1 mM calcium in solution for maximum nodule number, and M. murex only 0.5 mM calcium, whereas nodule number for M. truncatula increased up to 2 mM calcium, the highest concentration used. The results provide the basis for a simple screening system to distinguish differences among annual medics in nodulation tolerance to acidity. The maintenance of ranking among species with respect to nodulation over a wide range of stresses induced by combinations of low pH and calcium concentration suggest that screening using a single stress combining these two components would be adequate. Nodulation differences between species can be simply and effectively assessed using a scoring system combining number size and location of nodules.

Ambient pH and Calcium Concentration as Modifiers of Growth and Calcium Dynamics of Brook Trout, Salvelinus fontinalis

1984 ◽  
Vol 41 (12) ◽  
pp. 1774-1780 ◽  
Author(s):  
David W. Rodgers
Keyword(s):  
Brook Trout ◽  
Calcium Concentration ◽  
Salvelinus Fontinalis ◽  
Axial Skeleton ◽  
Low Ph ◽  
Fish Growth ◽  
Calcium Dynamics ◽  
Experimental Fish ◽  
Low Calcium ◽  
High Calcium

Juvenile brook trout, Salvelinus fontinalis, were maintained in water of pH 5.3 or 6.5 and calcium concentrations of 5 or 40 mg/L to determine the effects of these factors on fish growth and calcium dynamics. Growth rates varied more than twofold and were significantly reduced by both low ambient calcium concentration and low pH. In contrast, calcium dynamics of the fish were significantly affected by calcium concentration but not pH. Brook trout in low-calcium water retained less labeled dietary calcium and deposited less labeled calcium in axial skeleton and visceral tissues than fish in high-calcium water. Calcium concentrations of the skin and fins were slightly but significantly reduced among fish in low-calcium water, but neither pH nor ambient calcium concentration significantly affected ash content or calcium concentration of axial skeleton and visceral tissues of experimental fish.

Sorption of Eu(III) at feldspar/water interface: effects of pH, organic matter, counter ions, and temperature

2017 ◽  
Vol 105 (12) ◽  
Author(s):  
Ping Li ◽  
Hanyu Wu ◽  
Jianjun Liang ◽  
Zhuoxin Yin ◽  
Duoqiang Pan ◽  
...  
Keyword(s):  
Organic Matter ◽  
Outer Sphere ◽  
Potassium Feldspar ◽  
Low Ph ◽  
High Ionic Strength ◽  
Counter Ions ◽  
Effects Of Ph ◽  
Alkaline Conditions ◽  
Ph Range ◽  
Interface Effects

AbstractThe sorption of Eu(III) on potassium feldspar (K-feldspar) was studied under various physicochemical conditions such as pH, temperature, counter ions and organic matter. The results showed that the sorption of Eu(III) on K-feldspar significantly increased with the increase of pH, and high Eu(III) concentration can inhibit such immobility to some extent. The presence of humic acid (HA) can increase the sorption of Eu(III) on K-feldspar in low pH range; while inhibit to a large extent under alkaline conditions. It is very interesting that at pH ~6.5, high ionic strength can promote the sorption of Eu(III) on K-feldspar in the presence of HA. In contrast, Eu(III) sorption was restricted obviously by NaCl in the absence of HA. The sorption procedure was involved with ion exchange and/or outer-sphere complexation as well as inner-sphere complexation. The presence of F

scholarly journals Studies of the relationship between the catalytic activity and binding of non-substrate ligands by the glutathione S-transferases

1984 ◽  
Vol 217 (1) ◽  
pp. 179-185 ◽  
Author(s):  
T D Boyer ◽  
D A Vessey ◽  
C Holcomb ◽  
N Saley
Keyword(s):  
Indocyanine Green ◽  
Bile Acids ◽  
Low Ph ◽  
Glutathione S Transferase ◽  
Effects Of Ph ◽  
Glutathione S Transferases ◽  
Ph Range ◽  
The Individual ◽  
Induced Changes ◽  
The Relationship

The dimeric enzyme glutathione S-transferase B is composed of two dissimilar subunits, referred to as Ya and Yc. Transferase B (YaYc) and two other transferases that are homodimers of the individual Ya and Yc subunits were purified from rat liver. Inhibition of these three enzymes by Indocyanine Green, biliverdin and several bile acids was investigated at different values of pH (range 6.0-8.0). Indocyanine Green, biliverdin and chenodeoxycholate were found to be effective inhibitors of transferases YaYc and YcYc at low (pH 6.0) but not high (pH 8.0) values of pH. Between these extremes of pH intermediate degrees of inhibition were observed. Cholate and taurochenodeoxycholate, however, were ineffective inhibitors of transferase YcYc at all values of pH. The observed differences in bile acids appeared to be due, in part, to differences in their state of ionization. In contrast with the above results, transferase YaYa was inhibited by at least 80% by the non-substrate ligands at all values of pH. These effects of pH on the three transferases could not be accounted for by pH-induced changes in the enzyme's affinity for the inhibitor. Thus those glutathione S-transferases that contain the Yc subunit are able to act simultaneously as both enzymes and binding proteins. In addition to enzyme structure, the state of ionization of the non-substrate ligands may also influence whether the transferases can perform both functions simultaneously.

Efficient Preconcentration of Cu2+, Zn2+ and Fe3+ with an Agarose-Schiff Base Sorbent

2007 ◽  
Vol 72 (7) ◽  
pp. 908-916 ◽  
Author(s):  
Payman Hashemi ◽  
Hatam Hassanvand ◽  
Hossain Naeimi
Keyword(s):  
Schiff Base ◽  
Metal Ions ◽  
Metal Ion ◽  
Good Accuracy ◽  
Kinetic Studies ◽  
Sample Volume ◽  
Effects Of Ph ◽  
Glass Column ◽  
High Concentrations ◽  
Ph Range

Sorption and preconcentration of Cu2+, Zn2+ and Fe3+ on a salen-type Schiff base, 2,2'- [ethane-1,2-diylbis(nitrilomethylidyne)]bis(2-methylphenol), chemically immobilized on a highly crosslinked agarose support, were studied. Kinetic studies showed higher sorption rates of Cu2+ and Fe3+ in comparison with Zn2+. Half-times (t1/2) of 31, 106 and 58 s were obtained for sorption of Cu2+, Zn2+ and Fe3+ by the sorbent, respectively. Effects of pH, eluent concentration and volume, ionic strength, buffer concentration, sample volume and interferences on the recovery of the metal ions were investigated. A 5-ml portion of 0.4 M HCl solution was sufficient for quantitative elution of the metal ions from 0.5 ml of the sorbent packed in a 6.5 mm i.d. glass column. Quantitative recoveries were obtained in a pH range 5.5-6.5 for all the analytes. The volumes to be concentrated exceeding 500 ml, ionic strengths as high as 0.5 mol l-1, and acetate buffer concentrations up to 0.3 mol l-1 for Zn2+ and 0.4 mol l-1 for Cu2+ and Fe3+ did not have any significant effect on the recoveries. The system tolerated relatively high concentrations of diverse ions. Preconcentration factors up to 100 and detection limits of 0.31, 0.16 and 1.73 μg l-1 were obtained for Cu2+, Zn2+ and Fe3+, respectively, for their determination by a flame AAS instrument. The method was successfully applied to the metal ion determinations in several river water samples with good accuracy.

scholarly journals Common Loon, Gavia immer, Breeding Success in Relation to Lake pH and Lake Size Over 25 Years

2009 ◽  
Vol 123 (2) ◽  
pp. 146 ◽  
Author(s):  
Robert Alvo
Keyword(s):  
Breeding Success ◽  
Low Ph ◽  
Single Pair ◽  
Gavia Immer ◽  
Common Loon ◽  
Sulphur Deposition ◽  
Acidic Lakes ◽  
Ph Range ◽  
Lake Size ◽  
Critical Ph

I monitored Common Loon (Gavia immer) breeding success in relation to lake pH (range 4.0–8.5) between 1982 and 2007 on 38 single-pair lakes (5–88 ha) in the Sudbury, Ontario, area. No chicks fledged on lakes with pH < 4.4. Chicks fledged on lakes with slightly higher pH only if the lakes were relatively large. Acidic lakes became less acidic as sulphur dioxide emissions from the Sudbury smelters and sulphur deposition from other long-range sources decreased. Two lakes initially too acidic to support successful loon reproduction eventually had successful reproduction. One loon pair used two large acidic lakes (combined area 140 ha) connected by shallow rapids, and one of the adults made extremely long dives (average = 99 s) while foraging for the chicks. One chick died on that lake after apparently ingesting a very large food item; the lack of smaller items was attributed to the lake’s acidity. My results suggest that a shortage of food for chicks is the main reason why low pH reduces breeding success. I suggest that, for lakes without high levels of dissolved organic carbon (DOC), the critical pH for loon breeding success is approximately 4.3, and the suboptimal pH is approximately 4.4–6.0.

Effect of phorbol myristate acetate on secretion of parathyroid hormone

1988 ◽  
Vol 254 (1) ◽  
pp. E63-E70 ◽  
Author(s):  
J. J. Morrissey
Keyword(s):  
Protein Kinase C ◽  
Parathyroid Hormone ◽  
Protein Kinase ◽  
Membrane Fraction ◽  
Hormone Secretion ◽  
Myristate Acetate ◽  
Kinase C ◽  
Low Calcium ◽  
Protein Kinase C Activity ◽  
High Calcium

The influence of phorbol myristate acetate (PMA), an activator of protein kinase c, on the secretion of parathyroid hormone from collagenase-dispersed bovine parathyroid cells was tested. The cells were incubated at low (0.5 mM) or high (2.0 mM) concentrations of calcium in the medium, and the hormone secreted into the medium was measured by a radioimmunoassay that recognizes both intact and C-terminal fragments of hormone. At low calcium, the secretory rate averaged 32 +/- 3.8 ng.h-1.(10(5) cells)-1. The addition of 1.6 microM PMA did not affect secretion. At high calcium there was a significant suppression of secretion by 38% to 19.8 +/- 3 ng.h-1.(10(5) cells)-1. The addition of 1.6 microM PMA significantly stimulated hormone secretion to 35.8 +/- 8 ng.h-1.(10(5) cells)-1, a rate indistinguishable from low calcium. This stimulatory effect of PMA at high calcium was seen at PMA concentrations as low as 1.6 nM, did not occur with a biologically inactive 4 alpha-isomer of phorbol ester, and was independent of changes in cellular adenosine 3',5'-cyclic monophosphate levels. Examination of 32P-labeled phosphoproteins by two-dimensional gel electrophoresis revealed acidic proteins of approximately 20,000 and 100,000 Da that were phosphorylated at low and high calcium + 1.6 microM PMA but not at high calcium alone. The protein kinase c activity associated with the membrane fraction of parathyroid cells significantly decreased 40% when the cells were incubated at high vs. low calcium. The data suggest that calcium may regulate parathyroid hormone secretion through changes in protein kinase c activity of the membrane fraction of the cell and protein phosphorylation.

Effects of Plant Population on Sole-crop Cassava in Sierra Leone

1978 ◽  
Vol 14 (3) ◽  
pp. 239-244 ◽  
Author(s):  
W. Godfrey-Sam-Aggrey
Keyword(s):  
Sierra Leone ◽  
Yield Components ◽  
Dry Matter ◽  
Weight Ratio ◽  
Land Plant ◽  
Plant Population ◽  
Root Weight ◽  
Plant Populations ◽  
Sole Crop ◽  
Yield And Yield Components

SUMMARYEffects of plant population on mean yield and yield components of 2-year sole cassava crops were studied on Njala upland soils of Sierra Leone in two experiments. Increasing plant population of multi-shoot Cocoa cassava over 7000/ha decreased all the parameters studied except top/root weight ratio, which increased. The observed effects were attributed to competition for environmental resources, since area of land/plant unit decreased as plant population increased. The relations between plant populations and yields of fresh root and cortex dry matter were asymptotic, indicating that the respective yields were products of the vegetative phase of cropping.

scholarly journals Secretion and degradation of parathormone as a function of intracellular maturation of hormone pools

1979 ◽  
Vol 83 (3) ◽  
pp. 521-528 ◽  
Author(s):  
JJ Morrissey ◽  
DV Cohn
Keyword(s):  
Cyclic Amp ◽  
Half Life ◽  
Calcium Concentration ◽  
Hormone Secretion ◽  
Ionized Calcium ◽  
Dibutyryl Cyclic Amp ◽  
Low Calcium ◽  
High Calcium ◽  
Parathormone Secretion

The biosynthesis, processing, and secretion of parthormone and the effect of calcium on these processes were measured in dispersed porcine parthyroid cells incubated with [(35)S]methionine. Proparathormone was detected at 10 min, the earliest time measured, and was rapidly and apparently quantitatively converted to parathormone. The half-life of the prohomormone pool was 15 min. Secretion of parathormone was detected by 20 min. In pulse-chase experiments there was a period between 20 and 40 min during which the wave of newly-synthesized parathormone was secreted. After 40 min during little additional radioactive hormone was secreted, but dibutyryl cyclic AMP, an agent that can mobilize stored parathormone, when added to the incubation mixtures enhanced radioactive parathormone secretion but only after 60 min, although it increased net hormone secretion as determined by radioimmunoassay to the same extent at all times studied. When the ionized calcium concentration of the medium was lowered, more radioactive hormone was secreted at all times but the effect was greatest on that hormone that was synthesized less than 60 min previously ; however, net hormone secretion in contrast to radioactive hormone was enhanced equally at all intervals. These data could mean that the refractoriness to secretion of parathormone 40-60 min of age was related to maturation of secretory container preparatory to storage. Low calcium (0.5 mM) stimulated hormone secretion up to fivefold compared to high calcium (3.0 mM) but did not affect synthesis of parathormone or proparathormne or conversion of the latter to hormone. During processing at least 70 percent of the intracellular parathormone was lost, presumably through proteolysis and this degradation was greater at high calcium. These data have been interpreted in light of the concept that two secretable pools of parathormone exist within the parathyroid.

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