Influence of xylanase supplementation on the apparent metabolisable energy and ileal amino acid digestibility in a diet containing wheat and oats, and on the performance of three strains of broiler chickens

1999 ◽  
Vol 50 (7) ◽  
pp. 1159 ◽  
Author(s):  
V. Ravindran ◽  
L. I. Hew ◽  
G. Ravindran ◽  
R. J. Gill ◽  
P. H. Pittolo ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Broiler Chickens ◽  
Dry Matter ◽  
Performance Parameters ◽  
Ileal Digestibility ◽  
Commercial Broiler ◽  
Metabolisable Energy ◽  
Digesta Viscosity

A commercial xylanase product was assessed for its effects on the performance of, and apparent metabolisable energy (AME) and ileal amino acid digestibility in, 3 commercial broiler strains (Strain A, B, and C) fed on a diet containing wheat (407 g/kg) and oats (78 g/kg). Exogenous xylanase improved weight gains (P = 0.07) and feed/gain (P < 0.04) of broilers, irrespective of genotype. Performance parameters significantly differed among the broiler strains. Strains A and C consumed more (P < 0.04) feed, and grew faster (P < 0.01) and more efficiently (P = 0.07) than Strain B. Enzyme supplementation resulted in 2.8% improvement (P < 0.05) in the AME of the wheat-based diet. The AME tended to be higher (P = 0.09) with Strain A (13.46 MJ/kg dry matter) and Strain C (13.57 MJ/kg dry matter) than with Strain B (13.10 MJ/kg dry matter). The apparent ileal digestibility of all amino acids was 1–2 percentage units higher in birds fed on the enzyme-supplemented diet than in those fed on the unsupplemented diet (controls), but the differences were significant (P < 0.05) only for threonine, methionine, isoleucine, arginine, aspartic acid, serine, and glutamic acid. Highly significant (P < 0.001) strain effects were observed for apparent ileal digestibility of all amino acids, with Strain A recording the highest (0.807–0.945) and Strain B the lowest (0.710–0.912). Mean ileal digestibility coefficients of the 15 amino acids in wheat-based diets for Strains A, B, and C were 0.858, 0.791, and 0.828, respectively. Ileal digesta viscosity was low, and was not affected by dietary enzyme, suggesting that other factors were responsible for the observed improvements in bird performance.

scholarly journals Apparent ileal digestibility of amino acids and metabolisable energy value in grains for broilers

2008 ◽  
Vol 14 (4) ◽  
pp. 325 ◽  
Author(s):  
S. PERTTILÄ ◽  
J. VALAJA ◽  
T. JALAVA
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Broiler Chickens ◽  
Crude Protein ◽  
Dry Matter ◽  
Nitrogen Excretion ◽  
Ileal Digestibility ◽  
Metabolisable Energy ◽  
Feed Protein ◽  
Digestible Amino Acids

Using ileal digestible amino acids in feed optimising will intensify feed protein utilizing and decrease nitrogen excretion to the environment. The study determined the apparent ileal digestibility (AID) coefficients of amino acids in barley, wheat, oats, triticale, maize, and dehulled oats in the diets of 180 Ross broiler chickens (aged 24–35 days). The birds were fed semi-purified diets that contained grain as the sole protein source and chromium-mordanted straw as an indigestible marker. The AID coefficients of the nutrients were assessed using the slaughter technique, and the apparent metabolisable energy (AME) was determined using total excreta collection. The ileal digestibility of the dry matter and organic matter were the highest in maize. The AME of maize was higher than that of other cereals. The ileal digestibility of crude protein was higher in wheat than that in barley, oats and dehulled oats. The AME of wheat was similar to that of barley and oats but lower than that of triticale and dehulled oats. The amino acid AID was highest in wheat (0.86) and triticale (0.85) and lowest in oats (0.79) and barley 0.77). The average amino acid AID was 0.81 in dehulled oats. The threonine AID was the same in all tested ingredients. The lysine, methionine, and cystine AID coefficients were 0.81, 0.79, and 0.71 respectively for barley; 0.86, 0.84, and 0.38 respectively for oats; 0.87, 0.86, and 0.53 respectively for dehulled oats; 0.84, 0.90, and 0.66 respectively for maize; 0.89, 0.88, and 0.77 respectively for triticale; and 0.87, 0.85, and 0.71 respectively for wheat. Results indicated that AME –values of domestic grains (barley, oats and wheat) are in the same level. Especially, low AME value of wheat needs further investigation.;

scholarly journals A comparison of three xylanases on the nutritive value of two wheats for broiler chickens

2004 ◽  
Vol 92 (1) ◽  
pp. 53-61 ◽  
Author(s):  
M. Choct ◽  
A. Kocher ◽  
D. L. E. Waters ◽  
D. Pettersson ◽  
G. Ross
Keyword(s):  
Broiler Chickens ◽  
Elevated Level ◽  
Nutritive Value ◽  
Thermomyces Lanuginosus ◽  
Feed Conversion ◽  
Aspergillus Aculeatus ◽  
Ileal Digestibility ◽  
Humicola Insolens ◽  
Metabolisable Energy ◽  
Digesta Viscosity

Three xylanase products, xylanase A derived from Thermomyces lanuginosus, xylanase B from Humicola insolens and xylanase C from Aspergillus aculeatus, were examined for their effects on the nutritive value of wheat. The study investigated the effects of enzyme addition to broiler diets based on a low-metabolisable-energy (ME) wheat and a normal-ME wheat, with the emphasis on changes in composition of the NSP along the digestive tract in broiler chickens. There were significant (P>0·01) enzyme and wheat effects on the apparent ME, but there was no wheat×enzyme interaction on apparent ME. Weight gain and feed conversion efficiency (except xylanase C for normal wheat) were also significantly (P>0·01) increased by the xylanases. The enzymes differed in their effect on a number of important nutritional parameters. Xylanase A reduced (P>0·05) the excreta moisture level from 77·1% in birds fed the normal-ME wheat control to 73·4% and from 77·4% in those fed the low-ME wheat to 73·0%. The other two enzymes did not affect excreta moisture levels. The digesta viscosity in the duodenum, jejunum and ileum of birds fed both types of wheat was reduced (P>0·01) by xylanases A and C, but was increased (P>0·01) in the jejunum and ileum by xylanase B. The digestibility of the soluble NSP was mostly negative in the small intestine. The digestibility of the insoluble NSP differed (P>0·01) between the two wheats, with those in the low-ME wheat being more digestible (14 v. 28%). When supplemented with xylanase A, the ileal digestibility of the insoluble NSP in the normal- and low-ME wheats was increased by 28 and 42?%, respectively. Xylanases B and C did not affect the digestibility of the insoluble NSP regardless of wheat type. It may be concluded that wheats with low or normal ME values vary in their responses to xylanase supplementation. Apart from having an elevated level of soluble NSP, low-ME wheat may also contain insoluble NSP, which in the present study appeared to be more easily degradable in the gut of the chicken.

scholarly journals Amino acid digestibility coefficient values of animal protein meals with dietary protease for broiler chickens

2020 ◽  
Vol 4 (4) ◽  
Author(s):  
Antonio G Bertechini ◽  
Júlio C C de Carvalho ◽  
Andressa C Carvalho ◽  
Felipe S Dalolio ◽  
Jose O B Sorbara
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Broiler Chickens ◽  
High Specificity ◽  
Basal Diet ◽  
Weight Basis ◽  
Feather Meal ◽  
Collection Method ◽  
Ileal Digestibility ◽  
Poultry Byproducts

Abstract A digestibility experiment was conducted to evaluate the effects of dietary exogenous monocomponent protease on the coefficient of apparent total tract digestibility (ATTD) and coefficient apparent ileal digestibility (AID) of amino acids in meat and bones meal (MBM), poultry byproducts meal (PBPM), and feather meal (FM). A total of 512 Cobb-500 male broiler chickens (aged 14 d) were randomly placed into 64 metabolism cages (8 birds per pen) and were allocated to eight treatments with eight replicates in a semi-controlled environmental room. The experimental diets consisted of the basal diet (corn/SBM) and the replacement of 300 g/kg on a weight basis with MBM, PBPM, or FM. The excreta were collected during 3 d (19 to 21 d), and the ileal digesta (using Celite as an indigestible marker) only 1 d (21 d). The protease contained 75,000 PROT units/g. The use of the enzyme increased (P &lt; 0.05) ATTD for alanine, cysteine, glycine, and threonine in the basal diet and AID for the amino acids alanine, cysteine, glycine, lysine, threonine, and valine for the basal diet (vegetable). Regarding meals, there was an increase (P &lt; 0.05) in the amino acid digestibility in large part due to the amino acids of MBM (14) and PBPM (9), with only five amino acids for FM. The amino acids glycine and threonine showed increases (P &lt; 0.05) in both total and ileal digestibility for all animal meals and for the diet based on corn and soybean meal, which indicates a high specificity of the enzyme for these amino acids. The overall results obtained in this study found satisfactory effectiveness of this exogenous protease. The total collection method was lower by 1.83% of amino acids digestibility than the ileal method.

Studies on reproduction in prolific ewes:8. The concentrations and rates of accretion of amino acids in the foetuses

1985 ◽  
Vol 105 (1) ◽  
pp. 21-26 ◽  
Author(s):  
J. J. Robinson ◽  
I. McDonald ◽  
D. S. Brown ◽  
C. Fraser
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Gestational Age ◽  
Dry Matter ◽  
Microbial Protein ◽  
Foetal Life ◽  
Amino Acid Contents ◽  
The Individual

SUMMARYData are presented for the amino acid contents of 64 litters of Suffolk × (Finnish Landrace × Dorset Horn) foetuses varying in gestational age from 90 to 145 days and in litter size from one to five. The concentrations of glycine, cystine, arginine, proline and hydroxyproline in foetal dry matter increased with age, those for threonine, serine, glutamic acid, valine and methionine remained fairly constant while those for aspartic acid, phenylalanine, histidine and lysine tended to decrease. The most pronounced of these age effects were for cystine and hydroxyproline which increased in concentrations by 60 and 100% respectively over the final 2 months of foetal life. The rates of accretion of the individual amino acids are discussed in relation to estimates of the amounts that may be supplied by microbial protein.

Ileal digestibility of amino acids in pigs given a barley-based diet with increasing inclusion of lucerne leaf meal

1998 ◽  
Vol 67 (1) ◽  
pp. 131-138 ◽  
Author(s):  
M. Reverter ◽  
J. E. Lindberg
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Protein Intake ◽  
Crude Protein ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Growing Pigs ◽  
Ileal Digestibility ◽  
Leaf Meal

AbstractIleal digestibility of amino acids in barley-based diets with increasing inclusion of lucerne leaf meal (LLM; 0, 50, 100 and 200 g/kg) was studied in a change-over experiment with post-valve T-caecum cannulated growing pigs. The apparent Heal digestibility of crude protein, as well as of most of the essential (EAA) and non-essential amino acids (NEAA) was not significantly affected by the dietary inclusion of LLM. The exceptions were cystine, methionine, phenylalanine, glutamic acid and serine, all of which showed a significant (P< 0·05) reduction in apparent Heal digestibility with increasing inclusion of LLM. In contrast, the calculated true Heal digestibility of all EAA (with the exception of arginine and lysine) and the NEAA glutamic acid and serine were significantly (P< 0·05) reduced with the inclusion of LLM. Associated with an increased crude protein intake, there was a significant (P< 0·05) increase in the amount of absorbed EAA when the proportion of LLM was increased in the diet. With increasing proportion of fibre in the diet, there was a significant (P< 0·05) increase in the Heal flow of glucosamine.It can be concluded from the present data that the inclusion of LLM in a barley-based diet for growing pigs will result in an improvement in the absorbed amino-acid profile due to a significant increase in the absorption of all of the EAA.

scholarly journals Endogenous flow of amino acids in the avian ileum as influenced by increasing dietary peptide concentrations

2008 ◽  
Vol 101 (6) ◽  
pp. 822-828 ◽  
Author(s):  
Velmurugu Ravindran ◽  
Patrick C. H. Morel ◽  
Shane M. Rutherfurd ◽  
Donald V. Thomas
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Broiler Chickens ◽  
Amino Acid Profile ◽  
Dependent Manner ◽  
Endogenous Protein ◽  
High Concentrations ◽  
Protein Free Diet ◽  
Endogenous Amino Acid

The aim of the present study was to establish whether feeding broiler chickens with diets containing increasing dietary peptide concentrations would cause increases in ileal endogenous amino acid flow. The flow of N and most amino acids increased quadratically (P < 0·05 to 0·001) with increasing dietary concentrations of peptides. The exceptions were the flow of threonine, serine, glycine, tyrosine and cystine, which increased linearly (P < 0·001) with dietary peptide levels. Another notable exception to the general trend was the flow of proline, which was significantly higher (P < 0·01) in birds fed the protein-free diet. The amino acid profile of endogenous protein, expressed as proportion of crude protein, indicated that the ratios of threonine, glutamic acid, proline, glycine, leucine, histidine, arginine and cystine were influenced (P < 0·05) with increasing dietary peptide concentrations. In general, compared with the protein-free diet, the ratios of threonine and arginine in endogenous protein were lower (P < 0·05) and those of glutamic acid, glycine and histidine were greater (P < 0·05) in diets with high concentrations of peptides. The ratio of proline was found to decrease (P < 0·05) with increasing dietary peptide concentrations. These changes in the amino acid profile of endogenous protein are probably reflective of changes in the output of one or more of the components of endogenous protein. Overall, the present results demonstrated that increasing dietary peptide concentrations increased the flow of endogenous amino acid flow at the terminal ileum of broiler chickens in a dose-dependent manner and also caused changes in the composition of endogenous protein. The observed changes in endogenous amino flow will influence the maintenance requirements for amino acids and also have implications for the calculation of true digestibility coefficient of feedstuffs.

scholarly journals Differences in the amino acid composition of muscles from pheasant and broiler chickens

2006 ◽  
Vol 49 (5) ◽  
pp. 508-514 ◽  
Author(s):  
E. Straková ◽  
P. Suchý ◽  
F. Vitula ◽  
V. Večerek
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Broiler Chickens ◽  
Dry Matter ◽  
Nutritive Value ◽  
Matter Content ◽  
Dry Matter Content ◽  
Thigh Muscles

Abstract. The present paper explores amino acid composition of breast and thigh muscles of common pheasant and compares it with that in broiler chickens. The experimental feeding of both pheasant and broiler chickens proceeded for a period of 42 days at the identical conditions employing the same diet and rearing technology. Muscles were analysed for the content of following amino acids: Asp, Thr, Ser, Glu, Pro, Gly, Ala, Val, Met, Ile, Leu, Tyr, Phe, His, Lys, and Arg. The results show that the levels of most amino acids in thigh and breast muscles of pheasants (related to dry matter content) were significantly higher (P ≤ 0.01) than those in broiler chickens. In 42-day-old birds, the levels of individual amino acids in breast muscles (related to 100% of dry matter content) ranged from 8.02 to 127.32 g . kg-1 in pheasant chickens and from 19.77 to 110.33 g . kg-1 in broiler chickens while the corresponding average values in thigh muscles ranged from 14.77 g . kg-1 to 132.77 g . kg-1 in pheasant chickens and from 14.02 g . kg-1 to 93.53 g . kg-1 in broiler chickens. One interesting finding is that in the case of broiler chickens the levels of most amino acids in breast muscles were significantly higher (P ≤ 0.01) than those in thigh muscles. In the case of pheasant chickens, the difference between breast and thigh muscles was not confirmed which indicates that both muscles have high nutritive value. The results of amino acid composition of pheasant and broiler meat have proven a high nutritive value of pheasant meat in respect to human nutrition.

Standardized ileal amino acid digestibility in wheat, barley, pea and flaxseed for broiler chickens

2011 ◽  
Vol 91 (1) ◽  
pp. 103-111 ◽  
Author(s):  
A. Bandegan ◽  
A. Golian ◽  
E. Kiarie ◽  
R. L. Payne ◽  
G. H. Crow ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Broiler Chickens ◽  
Sole Source ◽  
Broiler Chicks ◽  
Test Diet ◽  
Nutrient Excretion ◽  
Ileal Digestibility ◽  
Coefficients Of Variation ◽  
The Mean

Bandegan A., Golian, A., Kiarie E., Payne R. L., Crow G. H., Guenter W. and Nyachoti C. M. 2011. Standardized ileal amino acid digestibility in wheat, barley, pea and flaxseed for broiler chickens. Can. J. Anim. Sci. 91: 103–111. Experiments were conducted to estimate ileal amino acids (AA) digestibility in six wheat (exp. 1; 288 birds), seven barley (exp. 2; 252 birds), five pea (exp. 3; 240 birds) and five flaxseed (exp. 4; 216 birds) samples in male Ross broiler chicks. Wheat, barley and pea were the sole source of dietary AA in exps. 1, 2 and 3, whereas, for exp. 4, flaxseed and wheat (50:50 wt/wt) or wheat provided dietary AA. Chromic oxide was the indigestible marker. Birds were fed chick starter for the first 14 d; on day 15 each test diet was randomly assigned to six cages, each with six (exps. 2 and 4) or eight (exps. 1 and 3) birds. On day 21, birds were killed and digesta were sampled to determine the apparent ileal AA digestibility (AID). The standardized ileal digestibility (SID) values were calculated using ileal endogenous AA losses previously determined in our laboratory from birds fed N-free diet. The mean SID values for indispensable AA ranged from 83.7% (Lys) to 93.8% (Phe), 80.4% (Arg) to 90.9% (Phe), 86.0% (Met+Cys) to 94.2% (Arg) and 47.4% (Thr) to 66.7% (Met) for wheat, barley, peas and flaxseed samples, respectively. Overall, SID AA estimates for flaxseed had higher (> 13%) coefficients of variation (CV) compared with other ingredients (CV<6%). These SID AA data will help nutritionists to formulate broiler diets that more closely match the birds' requirements and minimize nutrient excretion.

The effect ofin saccorumen incubation of a grass silage upon the total and D-amino acid composition of the residual silage dry matter

1984 ◽  
Vol 102 (3) ◽  
pp. 695-702 ◽  
Author(s):  
J. A. Rooke ◽  
H. A. Greife ◽  
D. G. Armstrong
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Dry Matter ◽  
Similar Proportion ◽  
Rumen Bacteria ◽  
Bacterial Protein ◽  
Grass Silage

SummaryGrass silage was incubated in polyester bags in the rumens of Jersey heifers for 2, 12, 24 and 48 h. The total (D + L) and D-amino acid contents of the silage and of the silage residues remaining after rumen incubation were determined. In addition, the contamination of the silage residues by rumen bacterial protein was measured by using35S as a marker of rumen bacterial protein. The amino acid profile of the residual silage dry matter differed markedly after 2 h of rumen incubation from that of the original silage; thereafter progressive changes in the amino acid composition of the residual silage dry matter occurred between 2 and 48 h of rumen incubation. The D-alanine content of the original silage was higher than that of D-glutamic acid. Both these D-amino acids disappeared almost completely from the silage after 2 h rumen incubation; between 2 and 48 h rumen incubation the quantities of D-alanine and D-glutamic acid in the residual silage dry matter increased. The residual silage dry matter contained more D-glutamic acid than D-alanine and these acids were in a similar proportion to that found in rumen bacteria; thus it was concluded that D-amino acids in the residual silage dry matter resulted from contamination of the residues by rumen bacteria. Contamination of residual silage protein by rumen bacterial protein increased with length of rumen incubation; the extent of contamination was similar for each incubation time whether assessed using35S or D-amino acids as markers of rumen bacterial protein. However, this contamination by rumen bacterial protein did not markedly alter the degradability of silage protein calculated from the disappearance of silage N incubatedin sacco.

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