Molecular Imaging of Red Blood Cells by Raman Spectroscopy

2011 ◽  
Vol 64 (5) ◽  
pp. 593 ◽  
Author(s):  
Bayden R. Wood ◽  
Paul R. Stoddart ◽  
Donald McNaughton
Keyword(s):  
Biological Sciences ◽  
Raman Spectroscopy ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Early Stage ◽  
Imaging Techniques ◽  
Excitation Power ◽  
Diagnostic Potential ◽  
Wide Range ◽  
Technical Advances

Raman spectroscopy allows visualization of 2D and 3D chemical distributions at high spatial resolution in a wide range of samples. It is insensitive to water, which makes it particularly attractive for applications in the biological sciences. At the same time, technical advances have allowed the laser excitation power to be reduced on thermally sensitive samples, without sacrificing acquisition times. This review highlights the analytical and diagnostic potential of Raman imaging techniques by reference to recent studies of red blood cells. In the case of red blood cells infected with low-pigmented forms of the malaria parasite, molecular images reveal sub-micron-inclusions of haemozoin, which suggests that the technique has potential for early-stage diagnosis of the disease.

scholarly journals An Evaluation of Morphological Changes and Deformability of Suspended Red Blood Cells Prepared Using Whole Blood with Different Hemoglobin Levels of Tibetans

2021 ◽  
pp. 1-10
Author(s):  
Rui Zhong ◽  
Dingding Han ◽  
Xiaodong Wu ◽  
Hong Wang ◽  
Wanjing Li ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Morphological Changes ◽  
Osmotic Fragility ◽  
Spherical Shape ◽  
Hypoxic Environment ◽  
Wide Range ◽  
Group A ◽  
Cell Membrane Protein

Background: The hypoxic environment stimulates the human body to increase the levels of hemoglobin (HGB) and hematocrit and the number of red blood cells. Such enhancements have individual differences, leading to a wide range of HGB in Tibetans’ whole blood (WB). Study Design: WB of male Tibetans was divided into 3 groups according to different HGB (i.e., A: >120 but ≤185 g/L, B: >185 but ≤210 g/L, and C: >210 g/L). Suspended red blood cells (SRBC) processed by collected WB and stored in standard conditions were examined aseptically on days 1, 14, 21, and 35 after storage. The routine biochemical indexes, deformability, cell morphology, and membrane proteins were tested. Results: Mean corpuscular volume, adenosine triphosphate, pH, and deformability were not different in group A vs. those in storage (p > 0.05). The increased rate of irreversible morphology of red blood cells was different among the 3 groups, but there was no difference in the percentage of red blood cells with an irreversible morphology after 35 days of storage. Group C performed better in terms of osmotic fragility and showed a lower rigid index than group A. Furthermore, SDS-PAGE revealed similar cross-linking degrees of cell membrane protein but the band 3 protein of group C seemed to experience weaker clustering than that of group A as detected by Western Blot analysis after 35 days of storage. Conclusions: There was no difference in deformability or morphological changes in the 3 groups over the 35 days of storage. High HGB levels of plateau SRBC did not accelerate the RBC change from a biconcave disc into a spherical shape and it did not cause a reduction in deformability during 35 days of preservation in bank conditions.

scholarly journals Carbon Based Nanodots in Early Diagnosis of Cancer

2021 ◽  
Vol 9 ◽  
Author(s):  
Gurpal Singh ◽  
Harinder Kaur ◽  
Akanksha Sharma ◽  
Joga Singh ◽  
Hema Kumari Alajangi ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Early Stage ◽  
Imaging Techniques ◽  
Graphene Quantum Dots ◽  
Carbon Quantum Dots ◽  
Diagnostic Methods ◽  
Early Diagnosis Of Cancer ◽  
Successful Treatment Outcome ◽  
Wide Range ◽  
Carbon Based

Detection of cancer at an early stage is one of the principal factors associated with successful treatment outcome. However, current diagnostic methods are not capable of making sensitive and robust cancer diagnosis. Nanotechnology based products exhibit unique physical, optical and electrical properties that can be useful in diagnosis. These nanotech-enabled diagnostic representatives have proved to be generally more capable and consistent; as they selectively accumulated in the tumor site due to their miniscule size. This article rotates around the conventional imaging techniques, the use of carbon based nanodots viz Carbon Quantum Dots (CQDs), Graphene Quantum Dots (GQDs), Nanodiamonds, Fullerene, and Carbon Nanotubes that have been synthesized in recent years, along with the discovery of a wide range of biomarkers to identify cancer at early stage. Early detection of cancer using nanoconstructs is anticipated to be a distinct reality in the coming years.

Red Blood Cells in Patients with Arrhythmias Using Optical Tweezers Raman Spectroscopy

2011 ◽  
Vol 48 (2) ◽  
pp. 021701
Author(s):  
吴智辉 Wu Zhihui ◽  
莫华 Mo Hua ◽  
黄代政 Huang Daizheng ◽  
陈朝旺 Chen Chaowang
Keyword(s):  
Raman Spectroscopy ◽  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells

scholarly journals Haemorheology in dilute, semi-dilute and dense suspensions of red blood cells

2019 ◽  
Vol 872 ◽  
pp. 818-848 ◽  
Author(s):  
Naoki Takeishi ◽  
Marco E. Rosti ◽  
Yohsuke Imai ◽  
Shigeo Wada ◽  
Luca Brandt
Keyword(s):  
Red Blood Cells ◽  
Intrinsic Viscosity ◽  
Blood Cells ◽  
Volume Fraction ◽  
Relative Viscosity ◽  
Shear Plane ◽  
Constitutive Law ◽  
Stable Mode ◽  
Volume Fractions ◽  
Wide Range

We present a numerical analysis of the rheology of a suspension of red blood cells (RBCs) in a wall-bounded shear flow. The flow is assumed as almost inertialess. The suspension of RBCs, modelled as biconcave capsules whose membrane follows the Skalak constitutive law, is simulated for a wide range of viscosity ratios between the cytoplasm and plasma,$\unicode[STIX]{x1D706}=0.1$–10, for volume fractions up to$\unicode[STIX]{x1D719}=0.41$and for different capillary numbers ($Ca$). Our numerical results show that an RBC at low$Ca$tends to orient to the shear plane and exhibits so-called rolling motion, a stable mode with higher intrinsic viscosity than the so-called tumbling motion. As$Ca$increases, the mode shifts from the rolling to the swinging motion. Hydrodynamic interactions (higher volume fraction) also allow RBCs to exhibit tumbling or swinging motions resulting in a drop of the intrinsic viscosity for dilute and semi-dilute suspensions. Because of this mode change, conventional ways of modelling the relative viscosity as a polynomial function of$\unicode[STIX]{x1D719}$cannot be simply applied in suspensions of RBCs at low volume fractions. The relative viscosity for high volume fractions, however, can be well described as a function of an effective volume fraction, defined by the volume of spheres of radius equal to the semi-middle axis of a deformed RBC. We find that the relative viscosity successfully collapses on a single nonlinear curve independently of$\unicode[STIX]{x1D706}$except for the case with$Ca\geqslant 0.4$, where the fit works only in the case of low/moderate volume fraction, and fails in the case of a fully dense suspension.

scholarly journals Direct detection of malaria infected red blood cells by surface enhanced Raman spectroscopy

2016 ◽  
Vol 12 (6) ◽  
pp. 1445-1451 ◽  
Author(s):  
Funing Chen ◽  
Briana R. Flaherty ◽  
Charli E. Cohen ◽  
David S. Peterson ◽  
Yiping Zhao
Keyword(s):  
Raman Spectroscopy ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Direct Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Cell-derived microparticles in haemostasis and vascular medicine

2009 ◽  
Vol 101 (03) ◽  
pp. 439-451 ◽  
Author(s):  
Laurent Burnier ◽  
Pierre Fontana ◽  
Brenda R. Kwak ◽  
Anne Angelillo-Scherrer
Keyword(s):  
Endothelial Cells ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Essential Role ◽  
Cell Types ◽  
Vascular Medicine ◽  
Disease Markers ◽  
Wide Range ◽  
Definition Of ◽  
Different Cell Types

SummaryConsiderable interest for cell-derived microparticles has emerged, pointing out their essential role in haemostatic response and their potential as disease markers, but also their implication in a wide range of physiological and pathological processes. They derive from different cell types including platelets – the main source of microparticles – but also from red blood cells, leukocytes and endothelial cells, and they circulate in blood. Despite difficulties encountered in analyzing them and disparities of results obtained with a wide range of methods, microparticle generation processes are now better understood. However, a generally admitted definition of microparticles is currently lacking. For all these reasons we decided to review the literature regarding microparticles in their widest definition, including ectosomes and exosomes, and to focus mainly on their role in haemostasis and vascular medicine.

A Numerical Study of Plasma Skimming in Small Vascular Bifurcations

1994 ◽  
Vol 116 (1) ◽  
pp. 79-88 ◽  
Author(s):  
G. Enden ◽  
A. S. Popel
Keyword(s):  
Red Blood Cells ◽  
Cross Section ◽  
Newtonian Fluid ◽  
Blood Cells ◽  
Numerical Study ◽  
Flux Ratio ◽  
Parent Vessel ◽  
Plasma Skimming ◽  
Wide Range

Owing in part to a plasma-skimming mechanism, the distribution of red blood cells (RBCs) into branches of microvascular bifurcations typically differs from the distribution of the bulk blood flow. This paper analyzes the plasma-skimming mechanism that causes phase separation due to uneven distribution of red blood cells at the inlet cross section of the parent vessel. In a previous study, the shape of the surface that divides the flow into the branches was found by numerical simulation of three-dimensional flow of a homogeneous Newtonian fluid in T-type bifurcations. Those findings are used in this study to determine, as a first approximation, the side-to-parent vessel RBC flux ratio and discharge hematocrit ratio as a function of corresponding flow ratios. Calculations are based on the assumption that RBCs move along streamlines of a homogeneous Newtonian fluid and are uniformly distributed within a concentric core at the inlet cross section of the parent vessel. The results of our calculations agree well for a wide range of flow parameters with experimental data from in vivo and in vitro studies.

scholarly journals Conversaziones 1951

1951 ◽  
Vol 9 (1) ◽  
pp. 138-143
Keyword(s):  
Biological Sciences ◽  
Medical Research ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Vice President ◽  
Living Cells ◽  
The Other ◽  
Freezing And Thawing ◽  
Men And Women ◽  
Rabbit Blood

The Society’s Conversaziones this year were held on Thursday, 24 May 1951 and on Thursday, 28 June 1951. At the former there were thirty exhibits and at the latter twenty-four. The exhibits ranged through physical and biological sciences and once again the Society gratefully acknowledges the contribution of the exhibitors towards making these evenings, when the Society is host to its many friends, so successful. At both Conversaziones two films were shown, one entitled ‘ The freezing and thawing of living cells ’ which illustrated the work of Mr C. Polge, Mr J. Smiles and Dr Audrey Smith of the National Institute for Medical Research, London : the other film was entitled ‘ Medical aspects of venomous snakes ’ made by the Wellcome Foundation Limited. The former film showed how suspensions of fowl spermatozoa and of rabbit blood cells in the presence of glycerol could be taken to temperatures of — 60° C and on raising the temperature, the spermatozoa resumed fertility and the red blood cells are preserved from haemolysis. The film describing the snakes was in colour : that part of the photography dealing with living snakes was made at the London Zoo—not without some interesting moments ! These films, and the exhibits for the first Soirée, were seen by some 250 scholars on the morning of Friday, 25 May. Sir David Brunt, Vice-President and Physical Secretary, welcomed these young men and women of science, and Professor Finch, Chairman of the Soiree Committee, addressed them on some of the exhibits which they were about to see. From communications received since, it is clearly evident that this invitation to science scholars in the London area is greatly appreciated.

scholarly journals Deformation and dynamics of red blood cells in flow through cylindrical microchannels

Soft Matter ◽  
2014 ◽  
Vol 10 (24) ◽  
pp. 4258-4267 ◽  
Author(s):  
Dmitry A. Fedosov ◽  
Matti Peltomäki ◽  
Gerhard Gompper
Keyword(s):  
Blood Flow ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Flow Resistance ◽  
Flow Conditions ◽  
Hydrodynamic Simulations ◽  
Wide Range ◽  
Flow Through ◽  
Cell Partitioning

The behavior of red blood cells (RBCs) in microvessels plays an important role in blood flow resistance and in the cell partitioning within a microcirculatory network. We employ mesoscopic hydrodynamic simulations to study the behavior and deformation of single RBCs in microchannels yielding the construction of diagrams of RBC shapes for a wide range of flow conditions.

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