Soil microbe Bacillus subtilis (GB03) induces biomass accumulation and salt tolerance with lower sodium accumulation in wheat

2014 ◽  
Vol 65 (5) ◽  
pp. 423 ◽  
Author(s):  
Jin-Lin Zhang ◽  
Mina Aziz ◽  
Yan Qiao ◽  
Qing-Qiang Han ◽  
Jing Li ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Bacillus Subtilis ◽  
Salt Tolerance ◽  
Biomass Accumulation ◽  
Dry Weight ◽  
Subtilis Strain ◽  
Nacl Solution ◽  
Tissue Mass ◽  
Wheat Seedlings ◽  
Sodium Accumulation

Bacillus subtilis strain GB03 has been shown to confer salt tolerance in Arabidopsis thaliana. In this study, the potential for GB03 to promote biomass accumulation and increase salt tolerance was investigated in wheat (Triticum aestivum). Soil-grown wheat seedlings were assayed for dry-weight increase. Endogenous Na+ and K+ contents were determined in plants with or without soil inoculation with GB03 along with 0, 25 or 100 mm NaCl solution added to the soil. We demonstrated that the introduction of GB03 in the soil triggered wheat biomass accumulation. Furthermore, GB03 improved salt tolerance as measured by increased tissue mass, lower Na+ accumulation and improved K+/Na+ ratio when GB03-inoculated plants were grown under elevated salt conditions. This study provides insight for the application of selected bacteria to monocot crops to combat saline toxicity.

scholarly journals Contribution of Ascorbate and Glutathione in Endobacteria Bacillus subtilis-Mediated Drought Tolerance in Two Triticum aestivum L. Genotypes Contrasting in Drought Sensitivity

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2557
Author(s):  
Dilara Maslennikova ◽  
Oksana Lastochkina
Keyword(s):  
Triticum Aestivum ◽  
Bacillus Subtilis ◽  
Drought Tolerance ◽  
Triticum Aestivum L ◽  
Subtilis Strain ◽  
Wheat Cultivars ◽  
Stress Markers ◽  
Drought Tolerant ◽  
Adaptation To Drought ◽  
Significant Activation

We evaluated the effect of endobacteria Bacillus subtilis (strain 10–4) as a co-inoculant for promoting plant growth and redox metabolism in two contrasting genotypes of Triticum aestivum L. (wheat): Ekada70 (drought tolerant (DT)) and Salavat Yulaev (drought susceptible (DS)) in early stages of adaptation to drought (12% PEG–6000). Results revealed that drought reduced growth and dramatically augmented oxidative stress markers, i.e., hydrogen peroxide (H2O2) and lipid peroxidation (MDA). Furthermore, the depletion of ascorbate (AsA) and glutathione (GSH), accompanied by a significant activation of ascorbate peroxidase (APX) and glutathione reductase (GR), in both stressed wheat cultivars (which was more pronounced in DS genotype) was found. B. subtilis had a protective effect on growth and antioxidant status, wherein the stabilization of AsA and GSH levels was revealed. This was accompanied by a decrease of drought-caused APX and GR activation in DS plants, while in DT plants additional antioxidant accumulation and GR activation were observed. H2O2 and MDA were considerably reduced in both drought-stressed wheat genotypes because of the application of B. subtilis. Thus, the findings suggest the key roles in B. subtilis-mediated drought tolerance in DS cv. Salavat Yulaev and DT cv. Ekada70 played are AsA and GSH, respectively; which, in both cases, resulted in reduced cell oxidative damage and improved growth in seedlings under drought.

scholarly journals Variable survival ability of rhizobacteria in cumin (Cuminum cyminum L.) rhizosphere

2016 ◽  
Vol 8 (3) ◽  
pp. 1699-1703
Author(s):  
Anurag Yadav ◽  
Kusum Yadav
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Rhizosphere Soil ◽  
Population Decline ◽  
Dry Weight ◽  
Bulk Soil ◽  
Subtilis Strain ◽  
Initial Population ◽  
Final Population ◽  
Better Than

A study was undertaken to compare the survival efficacy of two native, previously characterized bacterial biovars viz. Bacillus subtilis BCU5 and Pseudomonas fluorescens PCU17 with Bacillus subtilis strain MTCC1789 and Pseudomonas fluorescens strain MTCC4828, procured from Institute of Microbial Technology, Chandigarh,India in cumin rhizosphere and bulk soil. All the four bacterial types were made rifampicin resistant and the mutants were applied as inoculants at the dosage of 6 log, 7 log and 8 log colony forming units (cfu) g-1 dry soil weight in pots containing cumin seedlings. The cfu of rhizosphere and bulk soil of pots was observed per week for four weeks. The results show that the initial population decline is a common feature of bioinoculants. In rhizosphere and bulk soil, the native bacterial biovars survived better than their procured counterparts. The population of P. fluorescens strain MTCC4828r in rhizosphere soil declined faster and reached below detection limit whereas the P. fluorescens biovar PCUr rhizosphere final population dropped to 3.1 log, 2.9 log and 2.13 log cfu g-1 soil dry weight with 8 log, 7 log and 6 log cfu g-1 soil dry weight inoculum treatment, respectively. In contrast to P. fluorescens strain MTCC4828r, the population of B. subtilis strain MTCC1789r stabilized after some decline and was comparable with B. subtilis biovar BCU5 population. Study concludes that the inoculant population decline in soil was the result of lower microbial load carrying capacity of soil than the provided inoculum densities. Also, the native bacteria survived better than procured ones in rhizosphere soil.

Involvement of phytohormones in the development of interaction between wheat seedlings and endophytic Bacillus subtilis strain 11BM

2011 ◽  
Vol 59 (1) ◽  
pp. 134-140 ◽  
Author(s):  
A. A. Egorshina ◽  
R. M. Khairullin ◽  
A. R. Sakhabutdinova ◽  
M. A. Luk’yantsev
Keyword(s):  
Bacillus Subtilis ◽  
Subtilis Strain ◽  
Wheat Seedlings

Azospirillum inoculation in pregerminating wheat seeds

1996 ◽  
Vol 42 (1) ◽  
pp. 83-86 ◽  
Author(s):  
Cecilia M. Creus ◽  
Rolando J. Sueldo ◽  
Carlos A. Barassi
Keyword(s):  
Triticum Aestivum ◽  
Azospirillum Brasilense ◽  
Dry Weight ◽  
The Other ◽  
Distilled Water ◽  
Fresh Weight ◽  
Bacterial Concentration ◽  
Wheat Seedlings ◽  
Viable Cells ◽  
Wheat Seeds

Azospirillum cells were inoculated in pregerminating wheat during seed imbibition. Surface-sterilized seeds of Triticum aestivum cv. Buck Pucará were sequentially soaked for 3 h in water and 3 h in the inoculum of 3 × 108Azospirillum brasilense Sp 245 cells∙mL−1, to allow bacteria to enter during imbibition. Germination and seedling growth were accomplished in sterile distilled water at 20 °C, in the dark. To compare with more traditional methods based on plant–Azospirillum colonization after germination, seedlings from noninoculated seeds were inoculated in parallel by immersing roots in the same inoculum, for the same period of time. Autoclaved inocula were used as controls in all cases. We observed about 5 × 108Azospirillum cells∙g−1 fresh weight in 11-day-old wheat seedlings inoculated before or after seed germination. However, roots from seed-inoculated seedlings had higher both bacterial concentration and length. On the other hand, seeds inoculated during imbibition and dried to 14% water content retained 3.7 × 106 viable cells∙g−1 dry weight up to 27 days. Moreover, seeds stored for 30 days were not only able to germinate but also to harbor over 106 cells∙g−1 fresh weight in roots after 7 days growth. Here we present the possibility of obtaining in a simple and inexpensive way, seeds containing high numbers of viable Azospirillum cells, which could avoid the use of external carriers or adhesives.Key words: Azospirillum, wheat, inoculation.

scholarly journals Seed Priming with Endophytic Bacillus subtilis Modulates Physiological Responses of Two Different Triticum aestivum L. Cultivars under Drought Stress

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1810
Author(s):  
Oksana Lastochkina ◽  
Darya Garshina ◽  
Sergey Ivanov ◽  
Ruslan Yuldashev ◽  
Regina Khafizova ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Bacillus Subtilis ◽  
Drought Stress ◽  
Negative Impact ◽  
Growth Parameters ◽  
Triticum Aestivum L ◽  
Wheat Seedlings ◽  
Chl A ◽  
Drought Conditions ◽  
Germination And Growth

The protective effects against drought stress of the endophytic bacterium Bacillus subtilis 10-4 were measured by studying the priming response in two wheat (Triticum aestivum L.)—Ekada70 (E70) and Salavat Yulaev (SY)—lines, tolerant and susceptible to drought, respectively. B. subtilis 10-4 improved germination and growth parameters under normal conditions in both cultivars with the most pronounced effect observed in cv. E70. Under drought conditions, B. subtilis 10-4 significantly ameliorated the negative impact of stress on germination and growth of cv. E70, but had no protective effect on cv. SY. B. subtilis 10-4 induced an increase in the levels of photosynthetic chlorophyll (Chl) a, Chl b, and carotenoids (Car) in the leaves of cv. E70, both under normal and drought conditions. In cv. SY plants, bacterial inoculation decreased the contents of Chl a, Chl b, and Car under normal conditions, but pigment content were almost recovered under drought stress. B. subtilis 10-4 increased water holding capacity (WHC) of cv. E70 (but did not affect this parameter in cv. SY) and prevented the stress-induced decline in WHC in both cultivars. Notably, B. subtilis 10-4 increased endogenous salicylic acid (SA) concentration in both cultivars, especially in cv. E70. Moreover, B. subtilis 10-4 reduced drought-induced endogenous SA accumulation, which was correlated with the influence of endophyte on growth, indicating a possible involvement of endogenous SA in the implementation of B. subtilis-mediated effects in both cultivars. Overall, B. subtilis 10-4 inoculation was found to increase drought tolerance in seedlings of both cultivars, as evidenced by decreased lipid peroxidation, proline content, and electrolyte leakage from tissues of wheat seedlings primed with B. subtilis 10-4 under drought conditions.

Salt Tolerance Is Not Associated With the Sodium Accumulation of Two Maize Hybrids

1994 ◽  
Vol 21 (5) ◽  
pp. 675 ◽  
Author(s):  
GR Cramer ◽  
GJ Alberico ◽  
C Schmidt
Keyword(s):  
Salt Tolerance ◽  
Ion Transport ◽  
Growth Response ◽  
Dry Weight ◽  
Weight Basis ◽  
Ion Concentration ◽  
Na Transport ◽  
Maize Hybrids ◽  
Sodium Accumulation ◽  
Ion Concentrations

In this report, we test the hypothesis that Na+ accumulation in the shoot in maize is negatively correlated with salt tolerance. Salt tolerance is defined as a percentage of the control on a dry weight basis. Two hybrids (Pioneer hybrid 3578 and Pioneer hybrid 3772) differing widely in Na+ accumulation were compared. Plants were treated with two types of salinity for 15 days (80 mol m-3 NaCl or 80 mol m-3 NaCl plus 8.75 mol m-3 CaCl2). Ion concentrations (Na+, K+, Ca2+ and Cl-) were measured in the roots, stalks, sheaths and leaves of plants harvested every third day. Ion concentrations were significantly affected by the treatments. Na+ and Cl- concentrations increased with salinity treatments; K+ and Ca2+ concentrations decreased. Supplemental Ca2+ increased Ca2+ and decreased Na+ concentrations. Hybrid 3772 maintained very low Na+ concentrations in the shoots, whereas 3578 did not. The largest distinction between the hybrids was in the ability to transport Na+ to the shoot; hybrid 3578 transported Na+ at twice the rate of hybrid 3772. In general, ion transport to the shoot appeared to be a function of root ion concentration. This model could account for the effects of NaCl salinity and supplemental Ca2+ on ion transport, although Na+ transport was complicated by an apparent reabsorption mechanism in the root and mesocotyl. The lack of correlation of Na+ accumulation in the shoot and other ion parameters with growth indicated that the mineral nutrition of the plants was not correlated with salt tolerance. It was concluded that the growth response of maize to salinity was primarily affected by osmotic factors.

scholarly journals Tolerance of wheat seedlings to excess boron

Genetika ◽  
2008 ◽  
Vol 40 (1) ◽  
pp. 75-82 ◽  
Author(s):  
Milka Brdar ◽  
Ivana Maksimovic ◽  
Borislav Kobiljski ◽  
Tijana Zeremski-Skoric ◽  
Marija Kraljevic-Balalic
Keyword(s):  
Triticum Aestivum ◽  
Root Growth ◽  
Boron Content ◽  
Selection Criterion ◽  
Dry Weight ◽  
Triticum Aestivum L ◽  
Growth Suppression ◽  
Tolerance Mechanisms ◽  
Boron Tolerance ◽  
Wheat Seedlings

Present in excessive amounts, micronutrient boron may become toxic for plants. The aim of this study was to investigate boron tolerance in seedlings of 12 wheat (Triticum aestivum L.) genotypes treated with boric acid. Selection criterion was root growth suppression in the presence of boron, which varied between 15.2 (Apache) and 46.3% (Renan). Root lengths on control and root growth suppression on treatments were not correlated. Boron content and dry weight of seedlings varied in vast intervals in all groups on control, as well as on treatments, which is probably caused by different boron tolerance mechanisms. Root growth suppression in genotypes Apache, Trakija and Bezostaja 1 was less than 20% and they may be considered as boron tolerant. Renan, Fundulea 4, Magdalena, Pergamino Gaboto and Donjecka 48 are marked as boron sensitive owing to more than 30% shorter roots on boron treatments. Norin 10/Brevor 14, Radika, Zitarka and Mironovska 808 were medium tolerant to excess boron. .

Halophile plant growth-promoting rhizobacteria induce salt tolerance traits in wheat seedlings (Triticum aestivum L.)

Pedosphere ◽  
2020 ◽  
Vol 30 (5) ◽  
pp. 684-693 ◽  
Author(s):  
Maryam SAFDARIAN ◽  
Hossein ASKARI ◽  
Ghorbanali NEMATZADEH ◽  
Adriano SOFO
Keyword(s):  
Triticum Aestivum ◽  
Salt Tolerance ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Triticum Aestivum L ◽  
Wheat Seedlings ◽  
Plant Growth Promoting ◽  
Growth Promoting

Proteomic analysis on salicylic acid-induced salt tolerance in common wheat seedlings (Triticum aestivum L.)

2012 ◽  
Vol 1824 (12) ◽  
pp. 1324-1333 ◽  
Author(s):  
Guozhang Kang ◽  
Gezi Li ◽  
Beibei Zheng ◽  
Qiaoxia Han ◽  
Chenyang Wang ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Salicylic Acid ◽  
Salt Tolerance ◽  
Common Wheat ◽  
Proteomic Analysis ◽  
Triticum Aestivum L ◽  
Wheat Seedlings
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