Genetic diversity of Kensington mango in Australia

1996 ◽  
Vol 36 (2) ◽  
pp. 243 ◽  
Author(s):  
ISE Bally ◽  
GC Graham ◽  
RJ Henry
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Environmental Factors ◽  
Genetic Improvement ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Mangifera Indica ◽  
Genetic Variations ◽  
Oligonucleotide Primers

The genetic diversity of Kensington mangoes (Mangifera indica L.) was investigated using random amplified polymorphic DNA (RAPD) analysis. DNA was extracted from leaves of 27 'Kensington Pride', 2 'R2E2' and 1 seedling. RAPD analysis with 10 oligonucleotide primers allowed the scoring of 107 markers. The R2E2 trees (20% dissimilarity) and the seedling (10% dissimilarity) were distinct from the Kensington Pride. However, there was very little evidence of significant genetic variation within Kensington Pride selections. Fifteen of the selections were identical in all 107 markers. Only 2 selections, WEAN2 and ML2N1, differed by more than 5%. These plants provide the best options for use in genetic improvement of the Kensington Pride mango. Many of the differences found in Kensington mango orchards may be due to environmental factors not genetic variations.

scholarly journals ECOLOGY AND THE RAPD TECHNIQUES USED TO ASSESS THE GENETIC DIVERSITY IN PTEROLOBIUM HEXAPETALUM, A SCRAMBLING MEDICINAL SHRUB IN MARUTHAMALAI AND CHENNIMALAI HILLS, THE WESTERN GHATS

2019 ◽  
pp. 72-77 ◽  
Author(s):  
SHARMILA S. ◽  
AKILANDESWARI D. ◽  
RAMYA E. K. ◽  
MOWNIKA S.
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Tamil Nadu ◽  
Climatic Factors ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Climatic Conditions ◽  
Individual Bands ◽  
Reproductive Characters ◽  
Two Populations

Objective: To investigate the ecological and genetic diversity, climatic factors, edaphic factors morphological and reproductive characters and RAPD analysis of medicinal plant species Pterolobium hexapetalum in two hills viz., Maruthamalai (arid) and Chennimalai (very arid), which is located in Coimbatore and Erode districts, Tamil Nadu. Methods: The present research was carried out by using a random amplified polymorphic DNA (RAPD) analysis was made to determine the genetic variation between the two populations of the medicinal shrub, Pterolobium hexapetalum in an environmental gradient. Among the five primers tested, the OPN7 (80 %) and OPN17 (71.4 %) produced higher polymorphism was used in RAPD analysis. Results: The results of RAPD analysis showed the presence of 51 individual bands were formed, out of which, 29 were polymorphic bands which showed the existence of genetic variation between populations. A dendrogram was constructed based on Jaccard’s coefficient to determine the degree of genetic relationship among the two populations and analysed. The primers OPN7 and OPN17 were clustered together at a genetic distance level 10. Considering the elevation and proximity, the temperature ranges from 18 °C to 37.6 °C in Maruthamalai hill and 20 °C to 39.4 °C in Chennimalai hill. Conclusion: From the morphoecological studies the results indicated that both arid and very arid climatic conditions showed slight differences in their vegetative and reproductive characters.

Use of Random Amplified Polymorphic DNA (RAPD) to study genetic diversity within a population of blackfly, Simulium gravelyi from Palni hills, peninsular India

Biologia ◽  
2012 ◽  
Vol 67 (6) ◽  
Author(s):  
Sankarappan Anbalagan ◽  
Chinnapandi Bharathiraja ◽  
Jeyaraj Pandiarajan ◽  
Sundaram Dinakaran ◽  
Muthukalingan Krishnan
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Ecological Factors ◽  
High Elevation ◽  
Molecular Evidence ◽  
Ecological Diversity ◽  
Peninsular India ◽  
High Elevation Site

AbstractThe genetic variation of local subpopulations (Palni hills, India) of the blackfly Simulium gravelyi was surveyed at different altitudinal locations using random amplified polymorphic DNA (RAPD) analysis. By comparing the similarity of the bands produced by RAPDs, it may be concluded that there is variation within this population. These data suggest that the heterogeneity of band pattern of S. gravelyi steadily increased from low elevation to high elevation. Site 5 (1590 m altitude) was more polymorphic and it differed most from site 1 (290 m). This is established by the ecological diversity and statistical analyses (PCA) and predicting two major ecological factors of conductivity and current velocity. The speciation hypothesis is consistent with the molecular evidence, postulates a high elevational site 5 (1590 m) and a subsequent site 4 (1250 m).

Low levels of genetic diversity in red pine confirmed by random amplified polymorphic DNA markers

1992 ◽  
Vol 22 (9) ◽  
pp. 1332-1337 ◽  
Author(s):  
A. Mosseler ◽  
K.N. Egger ◽  
G.A. Hughes
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rapd Markers ◽  
Black Spruce ◽  
Random Amplified Polymorphic Dna ◽  
Red Pine ◽  
Gene Markers ◽  
Island Populations ◽  
Oligonucleotide Primers ◽  
Low Levels

Random amplified polymorphic DNA (RAPD) markers were used to characterize genetic variation in disjunct Newfoundland populations of red pine (Pinusresinosa Ait.) for comparison with individuals from throughout the mainland range of red pine. Red pine demonstrated a largely monomorphic profile for 69 arbitrary oligonucleotide primers. DNA samples from white spruce (Piceaglauca (Moench) Voss) and black spruce (Piceamariana (Mill.) B.S.P.) that were screened together with red pine for 11 oligonucleotide primers showed abundant polymorphisms, confirming the genetic heterogeneity that characterizes these Boreal Zone spruces. Results with RAPD markers correspond with genetic diversity estimates using isozyme gene markers for both spruce species and red pine. RAPD markers provided further confirmation of low levels of genetic variation for a random sample of the red pine genome. A period of between 8000 and 10 000 years of isolation on the island of Newfoundland has resulted in very little detectable genetic differentiation of island populations from mainland populations, and the mainland populations have not recovered from losses of genetic diversity following a hypothesized genetic bottleneck that may have been experienced during glacial episodes of the Holocene. The low levels of genetic variation observed in red pine demonstrate the long time periods required for recovery following a loss of genetic diversity in long-lived, long-generation organisms like trees.

Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 201-210 ◽  
Author(s):  
F. N. Wachira ◽  
R. Waugh ◽  
W. Powell ◽  
C. A. Hackett
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Southeast Asia ◽  
Camellia Sinensis ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Systematic Assessment ◽  
Tree Crop ◽  
Taxonomic Relationships

Camellia sinensis is a beverage tree crop native to Southeast Asia and introductions have been made into several nonindigenous countries. No systematic assessment of genetic variability in tea has been done anywhere. In this study, random amplified polymorphic DNA (RAPD) analysis was used to estimate genetic diversity and taxonomic relationships in 38 clones belonging to the three tea varieties, assamica, sinensis, and assamica ssp. lasiocalyx. Extensive genetic variability was detected between species, which was partitioned into between and within population components. Seventy percent of the variation was detected within populations. Analyses based on band sharing separated the three populations in a manner consistent with both the present taxonomy of tea and with the known pedigrees of some clones. RAPD analysis also discriminated all of the 38 commercial clones, even those which cannot be distinguished on the basis of morphological and phenotypic traits.Key words: genetic diversity, RAPDs, Camellia sinensis.

scholarly journals Genetic diversity within and between broodstocks of the white shrimp Litopenaeus vannamei (Boone, 1931) (Decapoda, Penaeidae) and its implication for the gene pool conservation

2007 ◽  
Vol 67 (4 suppl) ◽  
pp. 939-943 ◽  
Author(s):  
PD. Freitas ◽  
MR. Calgaro ◽  
PM. Galetti Jr.
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Litopenaeus Vannamei ◽  
Gene Pool ◽  
Rapd Analysis ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Brazilian Coast ◽  
The Pacific ◽  
Improvement Programs

Genetic variation within and between fifteen closed broodstock lines of the Pacific white shrimp Litopenaeus vannamei, reared at different hatcheries in the Brazilian coast, was assessed by RAPD analysis. Fifty two polymorphic loci were identified when a set of five decamer primers was used in PCR. The genetic diversity analysis within lines evidenced genetic variation loss probably related to bottleneck effects and inbreeding. In addition, the genetic divergence values between the different samples appear to reflect the initial founder composition of such stocks, in some cases, sharing a common origin, suggesting a putative importance of interbreeding for the establishment of genetic improvement programs for these broodstocks. The genetic variation monitoring appears to be helpful to the gene pool conservation of this aquaculture species, mainly if considered its exotic status in Brazil and the current impossibility of new introduction of wild individuals.

Comparative analysis of diversity based on morpho-metric and molecular markers in chickpea over different environments

2018 ◽  
Author(s):  
Indu Rialch ◽  
Rama Kalia ◽  
H. K. Chaudhary ◽  
B. Kumar ◽  
J. C. Bhandari ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Agronomic Traits ◽  
Rapd Analysis ◽  
Polymorphic Information Content ◽  
Random Amplified Polymorphic Dna ◽  
Similarity Coefficient ◽  
Breeding Programme ◽  
Metric Traits

Ten morpho-agronomic traits and 80 random amplified polymorphic DNA (RAPD) molecular markers were used to survey genetic diversity in 25 chickpea genotypes. Analysis of variance revealed significant variability among different genotypes for morpho-metric traits. The cluster analysis done using morpho-metric traits grouped 25 genotypes into seven and six clusters in Environment I (Env. I) and Environment II (Env. II), respectively. Three genotypes viz., ICCV-96904, HPG-17, ICCV-95503 and L-HR-1 belonging to diverse clusters were identified divergent and may use in heterosis breeding programme. Of 80 random RAPD markers, 25 were found polymorphic. Three major clusters were identified using 25 polymorphic RAPD markers. The genetic similarity coefficient among genotypes ranged from 0.57 to 0.91. The average polymorphic information content (PIC) for 25 RAPD markers ranges from 0.12 to 0.40. D2-statistic, RAPD analysis and study of genotypes performance revealed sufficient genetic diversity among chickpea genotypes which would be useful in future breeding programme.

scholarly journals Assessment of Genetic Diversity in Cultivated Tomato (Solanum Lycopersicum L.) Genotypes Using Rapd Primers

2013 ◽  
Vol 21 (1) ◽  
pp. 83-89 ◽  
Author(s):  
Saida Sharifova ◽  
Sabina Mehdiyeva ◽  
Konstantinos Theodorikas ◽  
Konstantinos Roubos
Keyword(s):  
Genetic Diversity ◽  
Rapd Analysis ◽  
Diversity Index ◽  
Random Amplified Polymorphic Dna ◽  
Arithmetic Mean ◽  
Similarity Coefficient ◽  
Group Method ◽  
Solanum Lycopersicum L ◽  
Pair Group ◽  
Upgma Cluster Analysis

Abstract Random Amplified Polymorphic DNA (RAPD) analysis was carried out on 19 Azerbaijan tomato genotypes, both cultivars and local populations. A total of 26 amplified products were revealed by 6 primers. The genetic similarity among evaluated genotypes ranged from 0.188 to 1.000. The lowest similarity was observed between cultivars ‘Azerbaijan’ and ‘Shakar’ (0.188), while the highest between ‘Elnur’ and ‘Garatag’ (1.000). The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analysis based on Jaccard’s similarity coefficient divided genotypes into four main groups. The first group was the largest and consisted of 12 genotypes, while the fourth group was the smallest consisted of 1 genotype only. The most polymorphic primer was OPB-18 that presented a genetic diversity index of 0.823, while the least informative was primer OPG-17 with an index of 0.349. The average genetic diversity calculated from RAPD data was 0.665.

scholarly journals Use of Random Amplified Polymorphic DNA Markers for Cultivar Identification in Mint

HortScience ◽  
2001 ◽  
Vol 36 (4) ◽  
pp. 761-764 ◽  
Author(s):  
A.L. Fenwick ◽  
S.M. Ward
Keyword(s):  
United States ◽  
Genetic Diversity ◽  
Dna Markers ◽  
Geographical Origin ◽  
Cultivar Identification ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
The United States ◽  
Randomly Amplified Polymorphic Dna ◽  
Commercial Oil

Seventeen mint accessions representing the three species grown for commercial oil production in the United States were characterized using randomly amplified polymorphic DNA (RAPD) analysis. The RAPD profiles readily identified the different Mentha species; calculation of genetic distance, based on the number of shared bands, indicated that M. spicata L. is more closely related to M. × gracilis than to M. × piperita. The RAPD profiles also distinguished among eight peppermint accessions of different geographical origin. However, only limited polymorphism was observed among the most widely grown peppermint and Scotch spearmint cultivars. These results indicate a potential lack of genetic diversity in mint cultivars grown for oil in the United States.

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