A calcium sensor-interacting protein kinase negatively regulates salt stress tolerance in rice (Oryza sativa)

2011 ◽  
Vol 38 (6) ◽  
pp. 441 ◽  
Author(s):  
Xiao-Lan Rao ◽  
Xiu-Hong Zhang ◽  
Rong-Jun Li ◽  
Hai-Tao Shi ◽  
Ying-Tang Lu
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Protein Kinase ◽  
Transgenic Plants ◽  
Stress Tolerance ◽  
Negative Regulator ◽  
Calcium Sensor ◽  
Salt Stress Tolerance ◽  
Interacting Protein ◽  
Salt Stress Response

Protein kinases are signal transduction factors that play a central role in acclimation. In this study, the function of a calcium sensor-interacting protein kinase, OsCIPK03, was characterised in the salt stress response of rice (Oryza sativa L.). Transgenic plants overexpressing OsCIPK03 exhibited an increased sensitivity to salt stress during both seed germination and seedling growth. By contrast, transgenic RNA interference lines that underexpressed OsCIPK03 were significantly more tolerant to NaCl stress than the wild-type. In response to salt stress, rice that underexpressed OsCIPK03 accumulated more proline than non-transformed plants. Furthermore, several stress-responsive genes were identified as being differentially expressed in the transgenic plants. Together, these results suggest that OsCIPK03 functions as a negative regulator of salt stress tolerance in rice.

scholarly journals TaCIPK29, a CBL-Interacting Protein Kinase Gene from Wheat, Confers Salt Stress Tolerance in Transgenic Tobacco

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e69881 ◽  
Author(s):  
Xiaomin Deng ◽  
Wei Hu ◽  
Shuya Wei ◽  
Shiyi Zhou ◽  
Fan Zhang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Protein Kinase ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Salt Stress Tolerance ◽  
Kinase Gene ◽  
Interacting Protein ◽  
Protein Kinase Gene

A maize calcineurin B‐like interacting protein kinase ZmCIPK42 confers salt stress tolerance

2020 ◽  
Vol 171 (1) ◽  
pp. 161-172
Author(s):  
Xunji Chen ◽  
Guo Chen ◽  
Jianping Li ◽  
Xiaoyan Hao ◽  
Zumuremu Tuerxun ◽  
...  
Keyword(s):  
Salt Stress ◽  
Protein Kinase ◽  
Stress Tolerance ◽  
Salt Stress Tolerance ◽  
Interacting Protein ◽  
Calcineurin B

scholarly journals Overexpression of Zinc Finger Transcription Factor ZAT6 Enhances Salt Tolerance

2018 ◽  
Vol 13 (1) ◽  
pp. 431-445 ◽  
Author(s):  
Wei Tang ◽  
Caroline Luo
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Protein Kinase ◽  
Stress Tolerance ◽  
Zinc Finger ◽  
Salt Stress Tolerance ◽  
Dependent Protein Kinase ◽  
Zinc Finger Transcription Factor ◽  
Transgenic Cell ◽  
Dependent Protein

AbstractThe purpose of the present investigation is to examine the function of the C2H2-type zinc finger transcription factor of Arabidopsis thaliana 6 (ZAT6) in salt stress tolerance in cells of rice (Oryza sativa L.), cotton (Gossypium hirsutum L.) and slash pine (Pinus elliottii Engelm.). Cells of O. sativa, G. hirsutum, and P. elliottii overexpressing ZAT6 were generated using Agrobacterium-mediated genetic transformation. Molecular and functional analysis of transgenic cell lines demonstrate that overexpression of ZAT6 increased tolerance to salt stress by decreasing lipid peroxidation and increasing the content of abscisic acid (ABA) and GA8, as well as enhancing the activities of antioxidant enzymes such as ascorbate peroxidise (APOX), catalase (CAT), glutathione reductase (GR), and superoxide dismutase (SOD). In rice cells, ZAT6 also increased expression of Ca2+-dependent protein kinase genes OsCPK9 and OsCPK25 by 5–7 fold under NaCl stress. Altogether, our results suggest that overexpression of ZAT6 enhanced salt stress tolerance by increasing antioxidant enzyme activity, hormone content and expression of Ca2+-dependent protein kinase in transgenic cell lines of different plant species.

scholarly journals Overexpression of a S-Adenosylmethionine Decarboxylase from Sugar Beet M14 Increased Araidopsis Salt Tolerance

2019 ◽  
Vol 20 (8) ◽  
pp. 1990 ◽  
Author(s):  
Meichao Ji ◽  
Kun Wang ◽  
Lin Wang ◽  
Sixue Chen ◽  
Haiying Li ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Transgenic Plants ◽  
Sugar Beet ◽  
Stress Tolerance ◽  
Membrane Damage ◽  
Pcr Analysis ◽  
Ros Generation ◽  
Salt Stress Tolerance ◽  
Adenosylmethionine Decarboxylase

Polyamines play an important role in plant growth and development, and response to abiotic stresses. Previously, differentially expressed proteins in sugar beet M14 (BvM14) under salt stress were identified by iTRAQ-based quantitative proteomics. One of the proteins was an S-adenosylmethionine decarboxylase (SAMDC), a key rate-limiting enzyme involved in the biosynthesis of polyamines. In this study, the BvM14-SAMDC gene was cloned from the sugar beet M14. The full-length BvM14-SAMDC was 1960 bp, and its ORF contained 1119 bp encoding the SAMDC of 372 amino acids. In addition, we expressed the coding sequence of BvM14-SAMDC in Escherichia coli and purified the ~40 kD BvM14-SAMDC with high enzymatic activity. Quantitative real-time PCR analysis revealed that the BvM14-SAMDC was up-regulated in the BvM14 roots and leaves under salt stress. To investigate the functions of the BvM14-SAMDC, it was constitutively expressed in Arabidopsis thaliana. The transgenic plants exhibited greater salt stress tolerance, as evidenced by longer root length and higher fresh weight and chlorophyll content than wild type (WT) under salt treatment. The levels of spermidine (Spd) and spermin (Spm) concentrations were increased in the transgenic plants as compared with the WT. Furthermore, the overexpression plants showed higher activities of antioxidant enzymes and decreased cell membrane damage. Compared with WT, they also had low expression levels of RbohD and RbohF, which are involved in reactive oxygen species (ROS) production. Together, these results suggest that the BvM14-SAMDC mediated biosynthesis of Spm and Spd contributes to plant salt stress tolerance through enhancing antioxidant enzymes and decreasing ROS generation.

scholarly journals Meta-Analysis of Salt Stress Transcriptome Responses in Different Rice Genotypes at the Seedling Stage

Plants ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 64 ◽  
Author(s):  
Weilong Kong ◽  
Hua Zhong ◽  
Ziyun Gong ◽  
Xinyi Fang ◽  
Tong Sun ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Meta Analysis ◽  
Calvin Cycle ◽  
Seedling Stage ◽  
Mitogen Activated Protein Kinase ◽  
Molecular Response ◽  
Salt Stress Tolerance ◽  
Salt Stress Response ◽  
Rice Genotypes

Rice (Oryza sativa L.) is one of the most important staple food crops worldwide, while its growth and productivity are threatened by various abiotic stresses, especially salt stress. Unraveling how rice adapts to salt stress at the transcription level is vital. It can provide valuable information on enhancing the salt stress tolerance performance of rice via genetic engineering technologies. Here, we conducted a meta-analysis of different rice genotypes at the seedling stage based on 96 public microarray datasets, aiming to identify the key salt-responsive genes and understand the molecular response mechanism of rice under salt stress. In total, 5559 genes were identified to be differentially expressed genes (DEGs) under salt stress, and 3210 DEGs were identified during the recovery process. The Gene Ontology (GO) enrichment results revealed that the salt-response mechanisms of shoots and roots were different. A close-knit signaling network, consisting of the Ca2+ signal transduction pathway, the mitogen-activated protein kinase (MAPK) cascade, multiple hormone signals, transcription factors (TFs), transcriptional regulators (TRs), protein kinases (PKs), and other crucial functional proteins, plays an essential role in rice salt stress response. In this study, many unreported salt-responsive genes were found. Besides this, MapMan results suggested that TNG67 can shift to the fermentation pathway to produce energy under salt stress and may enhance the Calvin cycle to repair a damaged photosystem during the recovery stage. Taken together, these findings provide novel insights into the salt stress molecular response and introduce numerous candidate genes for rice salt stress tolerance breeding.

scholarly journals OsPEX11, a Peroxisomal Biogenesis Factor 11, Contributes to Salt Stress Tolerance in Oryza sativa

2016 ◽  
Vol 7 ◽  
Author(s):  
Peng Cui ◽  
Hongbo Liu ◽  
Faisal Islam ◽  
Lan Li ◽  
Muhammad A. Farooq ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Stress Tolerance ◽  
Salt Stress Tolerance ◽  
Peroxisomal Biogenesis
Sign in / Sign up

Export Citation Format

Share Document