Identification of stay-green and early senescence phenotypes in high-yielding winter wheat, and their relationship to grain yield and grain protein concentration using high-throughput phenotyping techniques

2014 ◽  
Vol 41 (3) ◽  
pp. 227 ◽  
Author(s):  
Sebastian Kipp ◽  
Bodo Mistele ◽  
Urs Schmidhalter

Yield and grain protein concentration (GPC) represent crucial factors in the global agricultural wheat (Triticum aestivum L.) production and are predominantly determined via carbon and nitrogen metabolism, respectively. The maintenance of green leaf area and the onset of senescence (Osen) are expected to be involved in both C and N accumulation and their translocation into grains. The aim of this study was to identify stay-green and early senescence phenotypes in a field experiment of 50 certified winter wheat cultivars and to investigate the relationships among Osen, yield and GPC. Colour measurements on flag leaves were conducted to determine Osen for 20 cultivars and partial least square regression models were used to calculate Osen for the remaining 30 cultivars based on passive spectral reflectance measurements as a high-throughput phenotyping technique for all varieties. Using this method, stay-green and early senescence phenotypes could be clearly differentiated. A significant negative relationship between Osen and grain yield (r2 = 0.81) was observed. By contrast, GPC showed a significant positive relationship to Osen (r2 = 0.48). In conclusion, the high-throughput character of our proposed phenotyping method should help improve the detection of such traits in large field trials as well as help us reach a better understanding of the consequences of the timing of senescence on yield.

2001 ◽  
Vol 81 (3) ◽  
pp. 373-383 ◽  
Author(s):  
G. P. Lafond ◽  
Y. T. Gan ◽  
A. M. Johnston ◽  
D. Domitruk ◽  
F. C. Stevenson ◽  
...  

The recent advances in no-till seeding technology are providing new N management options for crop production on the prairies. The objectives of this study were to evaluate the potential interaction between P and N fertilizer on winter wheat production in a one-pass seeding and fertilizing system and to determine the feasibility of side-banding all N requirements using urea or anhydrous ammonia at planting as compared with the current practice of broadcasting ammonium nitrate early in the spring. Three forms of N fertilizer (urea, anhydrous ammonia, ammonium nitrate), three rates of N (50, 75 and 100 kg ha–1) and three rates of P (0, 9 and 17 kg P ha–1) were investigated. Urea and anhydrous ammonia were applied during the seeding operation, whereas ammonium nitrate was broadcast the following spring. Applying P fertilizer to the side and below the seed at planting with rates > 9 kg Pha–1 increased grain yield in 3 out of 6 site-years when ammonium nitrate was broadcast early in the spring. The positive yield response to P corresponded to soil test levels of 24 kg P ha–1. With soil test levels greater than 34 kg P ha–1, grain yield response to P fertilizer was not observed. When urea was banded at planting, together with P fertilizer, the yield increases with the increased P rates was shown only in 1 out of 6 site-years. At 5 of th e 6 site-years, grain protein concentration was not affected by P fertilizer; while for 1 site-year, the high rate of P fertilization decreased grain protein concentration. Responses of total grain N and P yields to P fertilization were parallel to the corresponding responses of P fertilization to grain yield, and were rarely associated with N or P concentrations in the grain. Applying N fertilizer at rates of 50 to 100 kg N ha–1 increased winter wheat grain yields by 3 to 8% in 3 out of 6 site-years. The high N rates increased grain protein concentrations in all 6 site-years. Grain protein concentration was 6% greater with N fertilizer applied as ammonium nitrate in early spring than when banding urea or anhydrous ammonia at planting. More consistent improvements in grain yield and grain protein concentration were obtained when the N fertilizer was applied as ammonium nitrate in the spring. Further research is required to determine the benefits of applying some of the crop’s N fertilizer requirements at planting, to reduce the risks of N stresses when the spring application is delayed because of adverse weather or soil conditions. Key words: Ammonium nitrate, anhydrous ammonia, grain yield, nitrogen timing, phosphorus, protein, urea


2010 ◽  
Vol 61 (15) ◽  
pp. 4303-4312 ◽  
Author(s):  
Matthieu Bogard ◽  
Vincent Allard ◽  
Maryse Brancourt-Hulmel ◽  
Emmanuel Heumez ◽  
Jean-Marie Machet ◽  
...  

1989 ◽  
Vol 69 (4) ◽  
pp. 1135-1147 ◽  
Author(s):  
M. H. ENTZ ◽  
D. B. FOWLER

Nitrogen and water are important variables that influence both grain yield and quality of wheat. The present study was conducted to investigate the combined effects of N and water on the growth, water use, yield and grain protein concentration of stubbled-in winter wheat produced in Saskatchewan. Seven field experiments were conducted between 1984 and 1986 on a range of soil types. Irrigation to approximately 150% of normal growing season precipitation significantly increased grain yield in five of the seven trials. A significant water × N interaction was recorded for grain yield in the remaining two trials. Grain yield response to irrigation averaged 10.9 kg ha−1 mm−1 at total available N levels above 140 kg ha−1. In trials where residual soil NO3–N to 61 cm averaged 40 kg ha−1, 62% of the grain yield increase was due to fertilizer N while 38% of the grain yield increase was due to the interaction between water and fertilizer N. Increases in grain yields due to N and water were attributed to increased levels of pre-anthesis dry matter, a higher number of kernels per square meter and an improved water use efficiency (kg ha−1 grain mm−1 total crop water use). The semidwarf cultivar Norwin and the tall cultivar Norstar responded similarly to N fertilization. In one trial, where moisture conditions were very favorable, a water × cultivar interaction indicated a greater response of Norwin to irrigation. The Gompertz equation was used to describe grain protein concentration-N response. The coefficient that describes the initial lag phase of this function was correlated with dry matter at anthesis (r = 0.97**) and root zone extractable water at stem elongation (r = 0.85**). These observations demonstrate that as pre-anthesis growing conditions improve more N is required to produce an increase in grain protein concentration above a minimum 8.2%.Key words: Wheat (winter), water use, nitrogen


2002 ◽  
Vol 82 (4) ◽  
pp. 489-498 ◽  
Author(s):  
B G McConkey ◽  
D. Curtin ◽  
C A Campbell ◽  
S A Brandt ◽  
F. Selles

We examined 1990-1996 crop and soil N data for no-tillage (NT), minimum tillage (MT) and conventional tillage (CT) systems from four long-term tillage studies in semiarid regions of Saskatchewan for evidence that the N status was affected by tillage system. On a silt loam and clay soil in the Brown soil zone, spring what (Triticum aestivum L.) grain yield and protein concentration were lower for NT compared with tilled (CT or MT) systems for a fallow-wheat (F-WM) rotation. Grain protein concentration for continuous wheat (Cont W) was also lower for NT than for MT. For a sandy loam soil in the Brown soil zone, durum (Triticum durum L.) grain protein concentration was similar for MT and NT for both Cont W and F-W, but NT had higher grain yield than MT (P < 0.05 for F-W only). For a loam soil in the Dark Brown soil zone, wheat grain yield for NT was increased by about 7% for fallow-oilseed-wheat (F-O-W) and wheat-oilseed-wheat (W-O-W) rotations. The higher grain yields for NT reduced grain protein concentration by dilution effect as indicated by similar grain N yield. However, at this site, about 23 kg ha-1 more fertilizer N was required for NT than for CT. Elimination of tillage increased total organic N in the upper 7.5 cm of soil and N in surface residues. Our results suggest that a contributing factor to decreased availability of soil N in medium- and fine-textured soils under NT was a slower rate of net N mineralization from organic matter. Soil nitrates to 2.4 m depth did not indicate that nitrate leaching was affected by tillage system. Current fertilizer N recommendations developed for tilled systems may be inadequate for optimum production of wheat with acceptable grain protein under NT is semiarid regions of Saskatchewan. Key words: Tillage intensity, N availability, soil N fractions, N mineralization, crop residue decomposition, grain protein


2002 ◽  
Vol 82 (3) ◽  
pp. 507-512 ◽  
Author(s):  
H. Wang ◽  
M. R. Fernandez ◽  
F. R. Clarke ◽  
R. M. DePauw ◽  
J. M. Clarke

Although leaf spotting diseases have been reported to have a negative effect on grain yield and seed characteristics of wheat (Triticum spp.), the magnitude of such effects on wheat grown on dryland in southern Saskatchewan is not known. A fungicide experiment was conducted at Swift Current (Brown soil) and Indian Head (Black soil) from 1997 to 1999 to determine the effect of leaf spotting diseases on yield and seed traits of wheat. Two fungicides, Folicur 3.6F and Bravo 500, were applied at different growth stages on three common wheat (Triticum aestivum L.) and three durum wheat (T. turgidum L. var durum) genotypes. Fungicide treatments generally did not affect yield, kernel weight, test weight or grain protein concentration, and these effects were relatively consistent among genotypes. Folicur applied at head emergence in 1997 and at flag leaf emergence and/or head emergence in 1998 increased yield at Indian Head (P < 0.05). Fungicides applied at and before flag leaf emergence tended to increase kernel weight. Grain protein concentration increased only in treatments of Bravo applications at Indian Head in 1998. These results suggested that under the dryland environment and management in southern Saskatchewan leaf spotting diseases generally have a small effect on yield, kernel weight, test weight and protein concentration. Key words: Wheat, leaf spotting diseases, fungicide, yield


1996 ◽  
Vol 36 (4) ◽  
pp. 443 ◽  
Author(s):  
MG Mason ◽  
RW Madin

Field trials at Beverley (19911, Salmon Gums (1991; 2 sites) and Merredin (1992; 2 sites), each with 5 rates of nitrogen (N) and 3 levels of weed control, were used to investigate the effect of weeds and N on wheat grain yield and protein concentration during 1991 and 1992. Weeds in the study were grasses (G) and broadleaf (BL). Weeds reduced both vegetative dry matter yield and grain yield of wheat at all sites except for dry matter at Merredin (BL). Nitrogen fertiliser increased wheat dry matter yield at all sites. Nitrogen increased wheat grain yield at Beverley and Merredin (BL), but decreased yield at both Salmon Gums sites in 1991. Nitrogen fertiliser increased grain protein concentration at all 5 sites-at all rates for 3 sites [Salmon Gums (G) and (BL) and Merredin (G)] and at rates of 69 kg N/ha or more at the other 2 sites [Beverley and Merredin (BL)]. However, the effect of weeds on grain protein varied across sites. At Merredin (G) protein concentration was higher where there was no weed control, possibly due to competition for soil moisture by the greater weed burden. At Salmon Gums (G), grain protein concentration was greater when weeds were controlled than in the presence of weeds, probably due to competition for N between crop and weeds. In the other 3 trials, there was no effect of weeds on grain protein. The effect of weeds on grain protein appears complex and depends on competition between crop and weeds for N and for water at the end of the season, and the interaction between the two.


2012 ◽  
Vol 4 (11) ◽  
Author(s):  
Ali Hafeez Malik ◽  
Allan Andersson ◽  
Ramune Kuktaite ◽  
Muhammad Yaqub Mujahid ◽  
Bismillah Khan ◽  
...  

1981 ◽  
Vol 21 (111) ◽  
pp. 424 ◽  
Author(s):  
WM Strong

Eighteen fertilizer trials, each with five levels of nitrogen (N) and three levels of phosphorus (PI, were conducted on black earth soils of the Darling Downs to establish optimal economic rates of N fertilizer in commercial, irrigated wheat crops. The optimal economic rate of N with a fertilizer: wheat price ratio (kg N: kg grain) of 5:l, the yield response of 100 kg/ha of applied N, the yield without fertilizer, and the yield with fertilizer not limiting were calculated from derived yield response relations at each site. A multi-variate regression procedure was used to determine which soil or crop management factors significantly influenced the rate of N needed to optimize wheat yield. Delay in planting after June 1 and the level of residual mineral N in the soil at planting had strong negative effects on the response to fertilizer and the optimal rate of fertilizer required. The results indicate that yields of irrigated wheat may be below the economic optimum because of sub-optimal applications of N. Other soil and management factors such as available soil P and number of irrigations also affected grain yield. At 1 3 sites low protein wheat (< 1 1.4�1~) was produced with all but the highest two rates of N fertilizer and at two sites even the highest rate produced low protein wheat. The effect of N fertilizer applied at planting on grain protein concentration was changed by the yield response to the fertilizer application. Grain protein concentration was curvilinearly related (R2 = 0.81) to relative grain yield (yield as a proportion of the maximum yield); grain protein was at its minimum at a relative yield of 0.5. Although heavy rates of N fertilizer at planting increased grain protein concentration on a few sites, usually these applications led to an inefficient use of N fertilizer; apparent incorporation of fertilizer N into grain decreased with increasing rate of fertilizer.


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