Hydroponic grown tobacco plants respond to zinc oxide nanoparticles and bulk exposures by morphological, physiological and anatomical adjustments

2019 ◽  
Vol 46 (4) ◽  
pp. 360 ◽  
Author(s):  
Maryam Mazaheri Tirani ◽  
Maryam Madadkar Haghjou ◽  
Ahmad Ismaili

Zinc oxide nanoparticles (NPs) are the third highest in terms of global production among the various inorganic nanoparticles, and there are concerns because of their worldwide availability and accumulation in the environment. In contrast, zinc is an essential element in plant growth and metabolism, and ZnO NPs (nano-ZnO) may have unknown interactions with plants due to their small sizes as well as their particular chemical and physical characteristics. The present study examined the effect of nano-ZnO (25nm) and bulk or natural form (<1000nm, bulk-ZnO), compared with zinc in the ionic form (ZnSO4) on Nicotiana tabacum seedlings in a nutrient solution supplemented with either nano-ZnO, bulk-ZnO (0.2, 1, 5 and 25µM) or ZnSO4 (control) for 21 days. Results showed that nano-ZnO at most of the levels and 1µM bulk-ZnO positively affected growth (root and shoot length/dry weight), leaf surface area and its metabolites (auxin, phenolic compounds, flavonoids), leaf enzymatic activities (CAT, APX, SOD, POX, GPX, PPO and PAL) and anatomical properties (root, stem, cortex and central cylinder diameters), while bulk-ZnO caused decreases at other levels. The activities of enzymes were induced to a greater extent by intermediate nano-ZnO levels than by extreme concentrations, and were higher in nano-ZnO treated than in bulk treated tobacco. As the ZnO level increased, the vascular expansion and cell wall thickening of the collenchyma/parenchyma cells occurred, which was more pronounced when treated by NPs than by its counterpart. The Zn content of root and leaf increased in most of ZnO treatments, whereas the Fe content of leaves decreased. Our findings indicate that tobacco responded positively to 1µM bulk-ZnO and to nearly all nano-ZnO levels (with the best levels being at 0.2µM and 1µM) by morphological, physiological and anatomical adjustments.

Zinc deficiency is one of the major malnutritional problems worldwide specifically in developing and developed countries. The zinc uptake takes place in human body in ionic form and metabolized naturally and converted into nano form. In the present study zinc oxide nanoparticles (ZnONPs) were synthesized by starch. The synthesized different ZnONPs were characterized by Fourier Transform Infra-Red spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) coupled with Energy Dispersive X ray analysis (EDX). FTIR analysis revealed Zinc peak at 405.05 cmˉ1 .The average size of the synthesized ZnONPs was in the range from 200-300 nm with flower like shape. Furthermore, toxicity and level of nanoparticle accumulation were evaluated in young and adult guppies (Poecilia reticulata) using different concentration of ZnONPs. To explore the toxicological effect, the different concentration of nanoparticles administered in young and adult guppies. There was a dose dependent toxicological effects were noted in the guppies.


2019 ◽  
Vol 8 (3) ◽  
pp. 2292-2294

Zinc oxide–Polyvinyl alcohol (ZnO/PVA) nanocomposites doped with varying concentrations of nano ZnO were prepared by solution casting method. Zinc oxide nanoparticles were synthesized by simple chemical route. Structural, Optical and Spectroscopic properties of the prepared nanocomposites were studied by using Ultra Violet Visible Spectrophotometer (UV-Vis), X-ray diffraction (XRD), Fourier Transform Infra Red Spectrophotometer (FTIR) and SEM techniques. XRD and FTIR are used to determine the effect of the synthetic conditions and resulting processing on the material structure. XRD reveals the presence of nano ZnO with hexagonal wurtzite phase in the polymer matrix. The peaks in FTIR spectra of the prepared films show the interaction between nano ZnO and the PVA polymer. SEM shows homogenous dispersion of zinc oxide nanoparticles in the polyvinyl alcohol matrix. The optical properties of ZnO/PVA nanocomposites have been investigated.


2014 ◽  
Vol 29 (10) ◽  
pp. 1039
Author(s):  
TAN Man-Lin ◽  
WANG Yan-Tao ◽  
ZHANG Wei-Li ◽  
FU Dong-Ju ◽  
LI Dong-Shuang ◽  
...  

Author(s):  
Hams H. H. Alfattli ◽  
Ghufran Zuhair Jiber ◽  
Ghaidaa Gatea Abbass

This study which designed to evaluate the inhibitory effect of Ethanolic extract of (Quercusrobur) and Zinc oxide nanoparticles on the growth of one genus of enterobacteriacae (Salmonella). In vitro. For this purpose graduate concentrates for plant extract (50, 100, 200, 400 )mg/ml which prepared and compared with Zinc oxide nanoparticles of different concentration (2, 1, 0.5, 0.25) μg/ml,and examined. The result showed that the studied medicinal plant has antibacterial activity against this bacteria which used. The result showed that the plant has good activity in decrease the growth of this bacteria. The results of the study also showed that the nano-ZnO has very effective antibacterial action against the studied bacteria which was Salmonella,nanoparticles concentrations lead to increasing in the inhibition zones of tested bacterial growth. We also study the effect of three antibiotics Lomefloxacin (LOM), Ciprofloxacin (SIP) and Rifampin (RA) and the result showed,in a comparison within the tested bacteria,Salmonella had a significant inhibition increase in Lomefloxacin ; the ciprofloxacin showed effect on tested bacteria. However,Rifampin does not show any effect on tested bacteria.


2019 ◽  
Vol 20 (7) ◽  
pp. 542-550 ◽  
Author(s):  
Nahla S. El-Shenawy ◽  
Reham Z. Hamza ◽  
Fawziah A. Al-Salmi ◽  
Rasha A. Al-Eisa

Background: Zinc oxide nanoparticles (ZnO NPs) are robustly used biomedicine. Moreover, no study has been conducted to explore the consequence of green synthesis of ZnO NPs with Camellia sinensis (green tea extract, GTE) on kidneys of rats treated with monosodium glutamate (MSG). Methods: Therefore, the objective of the research was designed to explore the possible defensive effect of GTE/ZnO NPs against MSG-induced renal stress investigated at redox and histopathological points. Results: The levels of urea and creatinine increased as the effect of a high dose of MSG, in addition, the myeloperoxidase and xanthine oxidase activates were elevated significantly with the high dose of MSG. The levels of non-enzymatic antioxidants (uric acid, glutathione, and thiol) were decreased sharply in MSG-treated rats as compared to the normal group. Conclusion: The data displayed that GTE/ZnO NPs reduced the effects of MSG significantly by reduction of the level peroxidation and enhancement intracellular antioxidant. These biochemical findings were supported by histopathology evaluation, which showed minor morphological changes in the kidneys of rats.


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