Evaluation of the Effect of Nanoparticles Zinc Oxide/Camellia sinensis Complex on the Kidney of Rats Treated with Monosodium Glutamate: Antioxidant and Histological Approaches

2019 ◽  
Vol 20 (7) ◽  
pp. 542-550 ◽  
Author(s):  
Nahla S. El-Shenawy ◽  
Reham Z. Hamza ◽  
Fawziah A. Al-Salmi ◽  
Rasha A. Al-Eisa
Keyword(s):  
Zinc Oxide ◽  
Camellia Sinensis ◽  
Zinc Oxide Nanoparticles ◽  
Morphological Changes ◽  
Monosodium Glutamate ◽  
Oxide Nanoparticles ◽  
High Dose ◽  
Enzymatic Antioxidants ◽  
Zno Nps ◽  
Tea Extract

Background: Zinc oxide nanoparticles (ZnO NPs) are robustly used biomedicine. Moreover, no study has been conducted to explore the consequence of green synthesis of ZnO NPs with Camellia sinensis (green tea extract, GTE) on kidneys of rats treated with monosodium glutamate (MSG). Methods: Therefore, the objective of the research was designed to explore the possible defensive effect of GTE/ZnO NPs against MSG-induced renal stress investigated at redox and histopathological points. Results: The levels of urea and creatinine increased as the effect of a high dose of MSG, in addition, the myeloperoxidase and xanthine oxidase activates were elevated significantly with the high dose of MSG. The levels of non-enzymatic antioxidants (uric acid, glutathione, and thiol) were decreased sharply in MSG-treated rats as compared to the normal group. Conclusion: The data displayed that GTE/ZnO NPs reduced the effects of MSG significantly by reduction of the level peroxidation and enhancement intracellular antioxidant. These biochemical findings were supported by histopathology evaluation, which showed minor morphological changes in the kidneys of rats.

The Interaction of Zinc Oxide/Green Tea Extract Complex Nanoparticles and its Effect on Monosodium Glutamate Toxicity in Liver of Rats

2019 ◽  
Vol 20 (6) ◽  
pp. 465-475 ◽  
Author(s):  
Fawziah A. Al-Salmi ◽  
Reham Z. Hamza ◽  
Nahla S. El-Shenawy
Keyword(s):  
Oxidative Stress ◽  
Zinc Oxide ◽  
Green Tea ◽  
Zinc Oxide Nanoparticles ◽  
Monosodium Glutamate ◽  
Green Tea Extract ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Tea Extract

Background: Zinc oxide nanoparticles (ZnO NPs) are increasingly utilized in both industrial and medical applications. Therefore, the study was aimed to investigate the effect of green nanoparticle complex (green tea extract/zinc oxide nanoparticles complex, GTE/ZnO NPs) on oxidative stress induced by monosodium glutamate (MSG) on the liver of rats. Methods: Wistar male rats (n=64) weighing between 200-250 g were divided randomly into eight groups: control group was given physiological saline (1 mg/kg), two groups were treated with two different doses of MSG (MSG-LD, MSG-HD; 6 and 17.5 mg/Kg, respectively), GTE was given 1 mg/mL, 5th group was treated with ZnO NPs and 6th group was treated with GTE/ZnO NPs complex while, 7th and 8th groups were treated with MSG-LD + GTE/ZnO NPs complex and MSG-HD + GTE/ZnO NPs complex, respectively. All substances were given orally for 30 consecutive days. At the end of the study, the liver was homogenized for measurement of the oxidative stress status and anti-inflammatory biomarkers as well as histological and transmission alternations. Results: Results showed that the antioxidant enzymes activity and glutathione level were significantly decreased in MSG groups than control in a dose-dependent manner. Conversely, the malondialdehyde and inflammatory cytokines levels were significantly increased in MSG groups than the control group. The liver indicated no evidence of alteration in oxidative status, anti-inflammatory and morphological parameters in GTE, ZnO NPs and GTE/ZnO NPs complex groups. Conclusion: In conclusion, MSG at both doses caused oxidative stress and inflammation on liver after 28 days of exposure that supported histological analysis and transmission view of hepatic parenchyma. GTE/ZnO NPs act as partial hepato-protective against MSG.

scholarly journals Evaluation of the effects of the green nanoparticles zinc oxide on monosodium glutamate-induced toxicity in the brain of rats

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7460 ◽  
Author(s):  
Reham Z. Hamza ◽  
Fawziah A. Al-Salmi ◽  
Nahla S. El-Shenawy
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Monosodium Glutamate ◽  
Food Additive ◽  
Control Treatment ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Neuronal Necrosis ◽  
Tea Extract ◽  
The Brain

Background Monosodium glutamate (MSG) is used extensively as a food additive in the diets of many countries around the world. Aim of the study Our aim was to determine the effects of green zinc oxide nanoparticles on MSG-induced oxidative damage, neurotransmitter changes, and histopathological alternation in the cerebral cortexes of rats. Methods MSG was administered orally at two doses of 6 and 17.5 mg/kg body weight. The higher dose was associated with a significant decline in the activities of superoxide dismutase, catalase, and glutathione peroxidase, as well as the levels of brain-derived neurotrophic factor (BDNF) and glutathione (GSH) in the cerebral cortex of rats. Results The administration of zinc oxide nanoparticles/green tea extract (ZnO NPs/GTE) to 17.5 mg/kg MSG-treated rats was associated with significant improvements in all parameters previously shown to be altered by MSG. The higher dose of MSG induced significant histopathological variation in brain tissue. Co-treatment of rats with ZnO NPs/GTE and MSG-HD inhibited the reduction of neurotransmitters and acetylcholinesterase by MSG. Conclusions ZnO NPs/GTE have the potential to protect against oxidative stress and neuronal necrosis induced by MSG-HD. ZnO NPs/GTE conferred a greater benefit than the control treatment or ZnO NPs or GTE administered separately.

Zinc oxide nanoparticles with green tea extract complex in the pancreas of rats against monosodium glutamate toxicity

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Reham Z. Hamza ◽  
Fawziah A. Al-Salmi ◽  
Nahla S. El-Shenawy
Keyword(s):  
Zinc Oxide ◽  
Green Tea ◽  
Zinc Oxide Nanoparticles ◽  
Monosodium Glutamate ◽  
Green Tea Extract ◽  
Glutamate Toxicity ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Pancreatic Cell ◽  
Tea Extract

AbstractObjectivesNanotechnology is an exciting field for investigators. Green zinc oxide nanoparticles (ZnO NPs) with Camellia sinensis extract complex are proved to be used in the treatment of the toxicity of monosodium glutamate (MSG) in the liver, kidney, and testis of rats. Therefore, the synthesized complex of green nanoparticles using green tea extract (GTE) was tested against the toxicity of MSG on the pancreas.MethodsThe glucose and insulin levels were estimated as well as some biochemical parameters for evaluating the antioxidant status of the pancreas tissue. The histopathological change of the pancreas also has been determined.ResultsIt indicates the biomedical capability of ZnO NPs/GTE to act as potent antidiabetic through decreasing blood glucose and increasing serum insulin also, inhibition of lipid peroxidation and enhancement of the antioxidant parameters.ConclusionsThe ZnO NPs/GTE enhanced the pancreatic cell and Langerhans islets as well lowered the sugar levels and stimulated insulin.

scholarly journals Cardioprotective Effect of Zinc Oxide Nanoparticles/Green Tea Extract Complex on Monosodium Glutamate Toxicity

2019 ◽  
pp. 1-5
Author(s):  
Nahla S. El-Shenawy ◽  
Reham Z. Hamza ◽  
Fawziah A. Al-Salmi
Keyword(s):  
Zinc Oxide ◽  
Body Weight ◽  
Green Tea ◽  
Zinc Oxide Nanoparticles ◽  
Monosodium Glutamate ◽  
Heart Tissue ◽  
Green Tea Extract ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Tea Extract

Monosodium glutamate (MSG) is used worldwide as a food additive, the survey has disclosed some of MSG's deleterious effects on various organs and tissues of rats. This research was achieved to determine the impact of MSG on the albino rats' antioxidant and histology of cardiac tissue. 48 male rats were divided into six groups of eight rats each. Group one used for monitoring and normal saline, whereas rats of group two were given the lower dosage of MSG 6 mg/Kg, rats in group three received 17.5 m/kg body weight of MSG, while rats in group four were given 10 mg/kg body weight of zinc oxide nanoparticles /green tea extract (ZnO NPs/GTE) complex, the fifth and sixth groups were treated with the lower and the higher doses of MSG with ZnO NP/GTE complex for 30 days. Each animal was sacrificed at the end of the treatment period and the heart was thoroughly separated, determining the antioxidant parameter and the histopathological changes. MSG administration to the rats has shown a substantial rise in peroxidation of cardiac heart tissue, decline in the activities of catalase, superoxide dismutase, and glutathione peroxidase beside the decrease in the glutathione level as compared to those in the control animals. The treatment the induced-rats with MSG using complex showed some degree of recovery by a reduction in LPO of the heart and enhancement of antioxidant enzymes. The findings suggested that the intrinsic antioxidant of the heart tissue can significantly be alternated with the use of MSG in a dose-dependent manner and these changes could be improved by using the green ZnO NPs complex. This complex acts as a factor to decrease the histological damage that could be happening by the induction of MSG.

scholarly journals Antioxidant Activities of Green Tea Extract/Zinc Oxide Nanoparticles Against Monosodium Glutamate in Different Organs

2020 ◽  
Vol 8 (1) ◽  
pp. 49-52
Author(s):  
Reham Z. Hamza ◽  
Fawzeh A. Al-Salmi ◽  
Nahla S. El-Shenawy
Keyword(s):  
Zinc Oxide ◽  
Green Tea ◽  
Zinc Oxide Nanoparticles ◽  
Antioxidant Activities ◽  
Monosodium Glutamate ◽  
Toxic Substance ◽  
Green Tea Extract ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Tea Extract

This brief review provided evidence for the role of green tea extract (GTE)/zinc oxide nanoparticles (ZnO NPs) in a variety of biomedical applications against the toxic effect of monosodium glutamate (MSG). MSG is used to enhance the taste, however it causes oxidative stress in the long-term. Many effects of MSG consumption on the brain, obesity, sex organs, and metabolism have been verified. This review covered the effect of GTE/ZnO NPs on many different organs including the liver, kidney, heart, spleen, testis, brain, and pancreas after being exposed to MSG. The review indicated that the toxicity induced by MSG could be restored by GTE/ZnO NPs in different organs. Accordingly, the green nanoparticles could be attended as a futuristic approach to be used against any toxic substance.

Genotoxicity evaluation of zinc oxide nanoparticles in Swiss mice after oral administration using chromosomal aberration, micronuclei, semen analysis, and RAPD profile

2017 ◽  
Vol 33 (11) ◽  
pp. 821-834 ◽  
Author(s):  
Anurag Kumar Srivastav ◽  
Akhilesh Kumar ◽  
Jyoti Prakash ◽  
Dhirendra Singh ◽  
Pankaj Jagdale ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Oral Administration ◽  
Chromosomal Aberration ◽  
Zinc Oxide Nanoparticles ◽  
Dose Group ◽  
High Dose Group ◽  
Oxide Nanoparticles ◽  
High Dose ◽  
Zno Nps ◽  
Swiss Mice

The expanded uses of zinc oxide nanoparticles (ZnO NPs) have grown rapidly in the field of nanotechnology. Thus, rising production of nanoparticles (NPs) increases the possible risks to the environment and occupationally exposed humans. Hence, it is indispensable to appraise the safety toxicity including genotoxicity for these NPs. In the present study, we have evaluated the genotoxic effect of ZnO NPs after oral administration to Swiss mice at dose levels of 300 and 2000 mg/kg body weight. These doses were administered for 2 days at 24 h apart. Chromosomal aberration (CA) and micronucleus tests were conducted following Organization for Economic Co-operation and Development guidelines. DNA damage was evaluated at 0, 24, 48, and 72 h posttreatment using a randomly amplified polymorphic DNA (RAPD) assay; additionally, semen analyses were also performed at 34.5 days post oral exposure. The reactive oxygen species (ROS), 8-oxo-2′-deoxyguanosine and CAs were increased ( p < 0.05) at the highest dosage (2000 mg/kg) of ZnO NPs compared to controls. Aberrant sperm morphology with reduced sperm count and motility were also present ( p < 0.05) in the high-dose group. Based on the RAPD assay, the genomic template stability within the high-dose group (<90%) was less than the controls (100%). The results suggested that ZnO NPs are mildly genotoxic in a dose-related manner and this toxicity were induced by generation of ROS.

scholarly journals Effect of Green Tea and Zinc oxide Nanoparticles Complex on Histopathology of Spleen of Male Rats Induced by Monosodium Glutamate

2019 ◽  
pp. 04-11
Author(s):  
Fawziah A Al-Salmi ◽  
Reham Z Hamza ◽  
Nahla S El-Shenawy
Keyword(s):  
Zinc Oxide ◽  
Green Tea ◽  
Zinc Oxide Nanoparticles ◽  
Monosodium Glutamate ◽  
Male Rats ◽  
Albino Rats ◽  
Oxide Nanoparticles ◽  
White Pulp ◽  
Zno Nps ◽  
Red Pulp

Background and objective: Synthesis of zinc oxide nanoparticles (ZnO NPs) with green tea extract (GTE) to form a complex is known to be one of the most multiuse nanoparticles with its application in treatment the toxicity of monosodium glutamate (MSG) on liver, kidney, testis, and pancreas. Therefore, the present study was concerned with the pontifical effect of ZnO NPs / GTE complex on the histological structure of spleen exposed to MSG. Materials and Methods: The toxicity of MSG was evaluated in male albino rats using two dosages (low, 6.0 and high, 17.5 mg/kg). The albino rats were taken for the experiment and randomly assigned into six groups; control, ZnO NPs, MSG-LD, MSG-HD, ZnO NPs / GTE + MSG-LD, and ZnO NPs / GTE + MSG-HD. The animals were decapitated after 30 days of exposure and spleens were dissected out and processed for the histological examination by light microscope. Results: The result revealed that MSG causes shrinkage in the white pulp nodule with increasing the area of the white pulp and degeneration of red pulp as compared to the control. The changes were more prominent in the rats treated with the higher dosage of MSG. The finding suggests that MSG may effect on adhesion of splenocytes and degeneration of red pulp in the rat leading to the reduced immunogenic response. Conclusions: The data could be demonstrated the effect of MSG on spleen tissue was a dose-dependent and led to hypertrophy of white pulp of the spleen. The ZnO NPs/GTE complex could provide a protective benefit against MSG-induced splenomegaly through its potent antioxidant properties due to the presence of GTE and reduction of the ZnO. The future study will be a concern on the thymus histology as it acts as the center of lymphoid organ. Keywords: Monosodium glutamate, ZnO nanoparticles, Spleen, Histology, Rats

scholarly journals Evaluation of the Toxicological Effects of Zinc Oxide Nanoparticles in Albino Male Mice

2020 ◽  
pp. 42-58
Author(s):  
Sarab Mohammed M. Razooki ◽  
Adel M. Rabee
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Seminiferous Tubules ◽  
Oxide Nanoparticles ◽  
High Dose ◽  
Dependent Manner ◽  
Male Mice ◽  
Zno Nps ◽  
Toxicological Effect ◽  
Histopathological Effects

The acute and sub chronic toxicity effects of 25.16 nm intraperitoneally- injected zinc oxide nanoparticles (ZnO NPs) were evaluated. Albino male mice were exposed to three different doses (25, 50 ,and 100 mg/kg ), depending on the value of calculated LD50, for 2 and 4 weeks . Considerable changes in organ indexes were shown with a good relevance to the illustrated histopathological effects which ranged from multiple hemorrhagic foci in liver, mild swelling and dilatation in kidney tubules, thickening of intestinal villi, moderate interstitial pneumonia, especially with the high dose , and sever necrosis of seminiferous tubules in testes of all studied groups. Significant changes in both hematological and biochemical parameters as well as thyroid hormones were observed with a considerable increase in the levels of antioxidant enzymes, in dose and exposure time dependent manner. The highest accumulated Zn mean values were recorded in the small intestine, kidney, liver, and spleen, respectively, followed by testes , heart , lung , and brain. These values followed the same order of the dose dependent manner, which explains the adverse effects that were recorded. This study proved the ability of using organ indexes as good tools side by side with the biochemical indicators to explain the histopathological changes. This study also revealed some histopathological effects that were not previously recorded as a toxicological effect of ZnO NPs in animal models.   

scholarly journals Ameliorating the Adverse Effects of Tomato mosaic tobamovirus Infecting Tomato Plants in Egypt by Boosting Immunity in Tomato Plants Using Zinc Oxide Nanoparticles

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1337
Author(s):  
Ahmed R. Sofy ◽  
Mahmoud R. Sofy ◽  
Ahmed A. Hmed ◽  
Rehab A. Dawoud ◽  
Abd El-Aleem M. Alnaggar ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Tomato Mosaic Virus ◽  
Systemic Resistance ◽  
Oxide Nanoparticles ◽  
Enzymatic Antioxidants ◽  
Growth Indices ◽  
Zno Nps ◽  
Tomato Plants ◽  
Challenge Control

Tomato mosaic virus (ToMV) is one of the economically damageable Tobamovirus infecting the tomato in Egypt that has caused significant losses. It is therefore of great interest to trigger systemic resistance to ToMV. In this endeavor, we aimed to explore the capacity of ZnO-NPs (zinc oxide nanoparticles) to trigger tomato plant resistance against ToMV. Effects of ZnO-NPs on tomato (Solanum lycopersicum L.) growth indices and antioxidant defense system activity under ToMV stress were investigated. Noticeably that treatment with ZnO-NPs showed remarkably increased growth indices, photosynthetic attributes, and enzymatic and non-enzymatic antioxidants compared to the challenge control. Interestingly, oxidative damage caused by ToMV was reduced by reducing malondialdehyde, H2O2, and O2 levels. Overall, ZnO-NPs offer a safe and economic antiviral agent against ToMV.

Zinc oxide nanoparticles help to enhance plant growth and alleviate abiotic stress: A review

2020 ◽  
Vol 21 ◽  
Author(s):  
Mohammad Faizan ◽  
Fangyuan Yu ◽  
Chen Chen ◽  
Ahmad Faraz ◽  
Shamsul Hayat
Keyword(s):  
Zinc Oxide ◽  
Plant Growth ◽  
Abiotic Stresses ◽  
Zinc Oxide Nanoparticles ◽  
Growth And Yield ◽  
Oxide Nanoparticles ◽  
Main Concern ◽  
Negative Effects ◽  
Zno Nps ◽  
Agricultural Yield

: Abiotic stresses arising from atmosphere change belie plant growth and yield, leading to food reduction. The cultivation of a large number of crops in the contaminated environment is a main concern of environmentalists in the present time. To get food safety, a highly developed nanotechnology is a useful tool for promoting food production and assuring sustainability. Nanotechnology helps to better production in agriculture by promoting the efficiency of inputs and reducing relevant losses. This review examines the research performed in the past to show how zinc oxide nanoparticles (ZnO-NPs) are influencing the negative effects of abiotic stresses. Application of ZnO-NPs is one of the most effectual options for considerable enhancement of agricultural yield globally under stressful conditions. ZnO-NPs can transform the agricultural and food industry with the help of several innovative tools in reversing oxidative stress symptoms induced by abiotic stresses. In addition, the effect of ZnO-NPs on physiological, biochemical, and antioxidative activities in various plants have also been examined properly. This review summarizes the current understanding and the future possibilities of plant-ZnO-NPs research.

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