Physiological and proteomic analyses of Tunisian local grapevine (Vitis vinifera) cultivar Razegui in response to drought stress

2021 ◽  
Author(s):  
Wassim Azri ◽  
Rahma Jardak ◽  
Pascal Cosette ◽  
Clément Guillou ◽  
Jawaher Riahi ◽  
...  
Author(s):  
Md Atikur Rahman ◽  
Iftekhar Alam ◽  
Shamima Akhtar Sharmin ◽  
Ahmad Humayan Kabir ◽  
Yong-Goo Kim ◽  
...  

PROTEOMICS ◽  
2010 ◽  
Vol 10 (14) ◽  
pp. 2661-2677 ◽  
Author(s):  
Sheng Zhang ◽  
Fugui Chen ◽  
Shuming Peng ◽  
Wujun Ma ◽  
Helena Korpelainen ◽  
...  

2014 ◽  
Vol 239 (6) ◽  
pp. 1067-1075 ◽  
Author(s):  
Chryssanthi Pateraki ◽  
Spiros Paramithiotis ◽  
Agapi I. Doulgeraki ◽  
Stamatina Kallithraka ◽  
Yorgos Kotseridis ◽  
...  

2013 ◽  
Vol 12 (11) ◽  
pp. 5110-5123 ◽  
Author(s):  
José Valero-Galván ◽  
Raquel González-Fernández ◽  
Rafael M Navarro-Cerrillo ◽  
Eustaquio Gil-Pelegrín ◽  
Jesús V. Jorrín-Novo

Plant Disease ◽  
2017 ◽  
Vol 101 (9) ◽  
pp. 1606-1615 ◽  
Author(s):  
Zhen-Hua Cui ◽  
Wen-Lu Bi ◽  
Xin-Yi Hao ◽  
Peng-Min Li ◽  
Ying Duan ◽  
...  

Reddish-purple coloration on the leaf blades and downward rolling of leaf margins are typical symptoms of grapevine leafroll disease (GLD) in red-fruited grapevine cultivars. These typical symptoms are attributed to the expression of genes encoding enzymes for anthocyanins synthesis, and the accumulation of flavonoids in diseased leaves. Drought has been proven to accelerate development of GLD symptoms in virus-infected leaves of grapevine. However, it is not known how drought affects GLD expression nor how anthocyanin biosynthesis in virus-infected leaves is altered. The present study used HPLC to determine the types and levels of anthocyanins, and applied reverse transcription quantitative polymerase chain reaction (RT-qPCR) to analyze the expression of genes encoding enzymes for anthocyanin synthesis. Plantlets of Grapevine leafroll-associated virus 3 (GLRaV-3)-infected Vitis vinifera ‘Cabernet Sauvignon’ were grown in vitro under PEG-induced drought stress. HPLC found no anthocyanin-related peaks in the healthy plantlets with or without PEG-induced stress, while 11 peaks were detected in the infected plantlets with or without PEG-induced drought stress, but the peaks were significantly higher in infected drought-stressed plantlets. Increased accumulation of total anthocyanin compounds was related to the development of GLD symptoms in the infected plantlets under PEG stress. The highest level of up-regulated gene expression was found in GLRaV-3-infected leaves with PEG-induced drought stress. Analyses of variance and correlation of anthocyanin accumulation with related gene expression levels found that GLRaV-3-infection was the key factor in increased anthocyanin accumulation. This accumulation involved the up-regulation of two key genes, MYBA1 and UFGT, and their expression levels were further enhanced by drought stress.


2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Yuan Cao ◽  
Qiuxiang Luo ◽  
Yan Tian ◽  
Fanjuan Meng

2011 ◽  
Vol 78 (2) ◽  
pp. 97-103 ◽  
Author(s):  
Stanisław Weidner ◽  
Monika Karolak ◽  
Magdalena Karamać ◽  
Ryszard Amarowicz

Grapevine seedlings<em> Vitis vinifera</em> were grown in a greenhouse under optimum conditions (soil moisture ca 70%) and under drought stress (soil moisture ca 35%). In addition, some of the plants subjected to drought underwent subsequent regeneration under optimum conditions. Drought stress caused accumulation of total phenolic compounds in grapevine roots, which may indicate that these compounds play an important role in the adaptation of roots to growth under stress conditions. Phenolic acids found in the roots occurred in the ester-bound form only. p-coumaric acid was present in the highest concentrations (6.2 to 10.5 µg/g fresh matter). The content of ferulic acid was lower, ranging from 2.4 to 4.6 µg/g fresh matter. The lowest concentration in grapevine roots was achieved by caffeic acid (2.4 to 2.9 µg/g fresh matter). The levels of p-coumaric and ferulic acids in roots rose significantly under the drought stress, while the concentration of caffeic acid increased during the post-drought recovery period. This may suggest that some of the phenolic acids protect plants under stress conditions. All the extracts from grapevine roots had antioxidative properties, but the antiradical activity of the extracts obtained from roots subjected to drought stress was inferior to the control. The same extracts were also characterised by depressed reducing power. The results imply that tolerance of grapevine to soil drought may be associated with the value of antioxidative potential in root tissues of these plants.


Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
M Halabalaki ◽  
S Bertrand ◽  
A Stefanou ◽  
J Boccard ◽  
S Kostidis ◽  
...  

2020 ◽  
Vol 61 (10) ◽  
pp. 1760-1774
Author(s):  
Jinjun Liang ◽  
Pingyin Guan ◽  
Zhenhua Liu ◽  
Yan Wang ◽  
Jiayi Xing ◽  
...  

Abstract Multicarpellate fruits are larger and produce more seeds than mono- or bicarpellate fruits, enhancing the reproductive capacity of the plant. To identify the phenotypic and molecular differences among florets of different carpel types, we studied carpel formation and fusion in the grapevine (Vitis vinifera) cultivar ‘Xiangfei’, which produces a high proportion of multicarpellate fruit. We also determined the function of VvSUPERMAN-like (VvSUP-like) and explored its relationship with VvWUS (VvWUSCHEL) and VvAG1 (VvAGAMOUS), which is related to the formation of carpel primordia. We showed that carpel formation and fusion were largely consistent between bicarpellate and tricarpellate ovaries, which both involve congenital fusion; rather, the differences between these ovary types arose from variation in carpel primordia number and location. Transgenic tomato (Solanum lycopersicum) plants expressing VvSUP-like produced significantly fewer carpels and other floral organs than the wild type. Moreover, transcriptome sequencing results indicate that VvSUP-like was more highly expressed in bicarpellate than in tricarpellate ‘Xiangfei’ florets. Luciferase reporter assays indicated that VvSUP-like inhibits the expression of VvAG1 and VvWUS by directly binding to their promoters, and VvWUS promotes VvAG1 expression by directly binding to its promoter. VvSUP-like inhibits the feedback signaling between VvWUS and VvAG1. Together, these results suggest that VvSUP-like negatively regulates the number of carpels that develop by inhibiting VvAG1 and VvWUS expression.


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