Effect of exogenous gonadotrophins on ovarian morphology and oocyte maturation in the southern hairy nosed wombat Lasiohinus latifrons during the breeding season

2006 ◽  
Vol 18 (4) ◽  
pp. 477 ◽  
Author(s):  
C. H. McDonald ◽  
D. A. Taggart ◽  
W. G. Breed ◽  
G. V. Druery ◽  
G. A. Shimmin ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
Follicular Development ◽  
Germinal Vesicle ◽  
Control Group ◽  
Antral Follicles ◽  
Nuclear Maturation ◽  
Significant Difference ◽  
Ovarian Morphology ◽  
The Mean ◽  
The Right

The effect of the exogenous administration of porcine follicle-stimulating hormone (pFSH) and pregnant mare serum gonadotrophin (PMSG) on ovarian follicular development and oocyte maturation in the southern hairy nosed wombat Lasiorhinus latifrons was investigated. Three experimental groups were administered pFSH at various doses and for different treatment lengths, followed by 25 mg porcine luteinising hormone (pLH) 12 h after the last dose of pFSH. Another group was given PMSG followed 72 h later by 25 mg pLH. Animals were killed 24 h after pLH. The left ovary was fixed for histology and the morphology of the antral follicles was determined, whereas follicular oocytes in the right ovary were aspirated, fixed, stained with 4′,6′-diamidino-2-phenylindole, and viewed for nuclear maturation. There was no significant difference in the mean number of ovarian follicles >1 mm, or in the size class of follicles assessed between control and experimental groups. However, a trend was observed suggesting a possible increase in follicles >3.0 mm in experimental groups compared with control animals. In all females administered exogenous porcine gonadotrophins, but not controls, some of the mural granulosa cells of large tertiary antral follicles had markedly enlarged nuclei (approximately 14 µm in diameter). All oocytes from the control group remained at the germinal vesicle stage, whereas approximately 40% of oocytes retrieved from the pFSH groups and 82.4% retrieved from the PMSG-primed animals had undergone germinal vesicle break down, with a small number reaching meiosis II. The present study shows that exogenous administration of either pFSH or PMSG to hairy nosed wombats can induce follicular growth and oocyte maturation. Such findings could be useful in the development of reproductive technology in this species.

scholarly journals Effect of Hyaluronan on Developmental Competence and Quality of Oocytes and Obtained Blastocysts fromIn VitroMaturation of Bovine Oocytes

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Jolanta Opiela ◽  
Joanna Romanek ◽  
Daniel Lipiński ◽  
Zdzisław Smorąg
Keyword(s):  
Dna Fragmentation ◽  
Oocyte Maturation ◽  
Developmental Competence ◽  
Meiotic Maturation ◽  
Nuclear Maturation ◽  
Significant Difference ◽  
The Mean ◽  
Bovine Oocytes

The objective of the present study was to evaluate the effect of hyaluronan (HA) during IVM on meiotic maturation, embryonic development, and the quality of oocytes, granulosa cells (GC), and obtained blastocysts. COCs were maturedin vitroin control medium and medium with additional 0.035% or 0.07% of exogenous HA. The meiotic maturity did not differ between the analysed groups. The best rate and the highest quality of obtained blastocysts were observed when 0.07% HA was used. A highly significant difference (P<0.001) was noted in the mean number of apoptotic nuclei per blastocyst and in the DCI between the 0.07% HA and the control blastocysts (P<0.01). Our results suggest that addition of 0.035% HA and 0.07% HA to oocyte maturation media does not affect oocyte nuclear maturation and DNA fragmentation. However, the addition of 0.07% HA during IVM decreases the level of blastocysts DNA fragmentation. Finally, our results suggest that it may be risky to increase the HA concentration during IVM above 0.07% as we found significantly higherBaxmRNA expression levels in GC cultured with 0.07% HA. The final concentration of HA being supplemented to oocyte maturation media is critical for the success of the IVP procedure.

Does Unilateral Cleft Lip and Palate Affect the Maxillary Sinus Volume?

2017 ◽  
Vol 55 (2) ◽  
pp. 168-172 ◽  
Author(s):  
Omer Demirtas ◽  
Fahrettin Kalabalik ◽  
Asim Dane ◽  
Ali Murat Aktan ◽  
Ertugrul Ciftci ◽  
...  
Keyword(s):  
Maxillary Sinus ◽  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Control Group ◽  
Maxillary Sinus Volume ◽  
Intergroup Comparison ◽  
Significant Difference ◽  
The Mean ◽  
And Gender ◽  
The Right

Objective: The purposes of this study were to evaluate and compare the maxillary sinus volume (MSV) of patients with a unilateral cleft lip and palate (UCLP) between the cleft side and noncleft side and between adolescent patients with UCLP and a control (noncleft) group using cone beam computed tomography (CBCT). Methods: CBCT images of 44 UCLP patients (29 males and 15 females, with a mean [SD] age of 13.5 [5.0] years) and 44 (22 males and 22 females, with a mean [SD] age 14.9 [4.2] years) age- and sex-matched controls were evaluated in this study. Each maxillary sinus was assessed 3-dimensionally, segmented, and its volume was calculated. Results: There were no statistically significant differences between the age and gender distributions of the groups. There was a statistically significant difference in the MSVs of the cleft (10996.78±3522.89 mm3) versus the noncleft side (10382.3±3416.2 mm3; P < .05)] but no significant difference between the MSVs of the right and left sides ( P > .05). In the intergroup comparison, the mean MSVs of the UCLP patients (10701.52±3369.33 mm3) were significantly smaller than those of the control group (16054.08 ± 5293.96 mm3; P < .001). Conclusions: The MSVs of the UCLP patients showed a statistically significant decrease compared to those of the controls ( P < .001). There was also a significant difference in the MSVs of the cleft and noncleft sides of the UCLP patients ( P < .05).

Electroretinogram B-Wave Amplitude in Panic Disorder

CNS Spectrums ◽  
2001 ◽  
Vol 6 (3) ◽  
pp. 210-213 ◽  
Author(s):  
Paolo Castrogiovanni ◽  
Fulvio Pieraccini ◽  
Sonia Iapichino ◽  
Claudia Pacchierotti ◽  
Letizia Bossini ◽  
...  
Keyword(s):  
Panic Disorder ◽  
Wave Amplitude ◽  
Dopaminergic System ◽  
Bright Light ◽  
Light Response ◽  
Control Group ◽  
Significant Difference ◽  
The Mean ◽  
The Right ◽  
Retinal Response

AbstractAbnormal light-related behaviors have been described for patients with panic disorder (PD). The present study was undertaken to investigate the retinal light response in PD using electroretinography (ERG). The authors conducted bwave ERG measurements with a bright light (after dark adaptation) in 28 patients with PD and 28 control subjects. There were no significant differences in the mean b-wave amplitude between the two groups, but the retinal response to light in PD patients was generally lower than in healthy subjects. A large interindividual variability was found; also noted was a significant difference in the mean b-wave amplitude between the right and left eyes in the control group. The data indicate subtle variation of retinal photosensitivity in a subgroup of patients with PD. Because dopaminergic retinal activity affects b-ERG amplitude, the authors hypothesize that the dopaminergic system is involved in the response to light in PD patients.

scholarly journals Can the coronavirus disease 2019 (COVID-19) cause choroiditis and optic neuropathy?

2021 ◽  
Vol 19 ◽  
pp. 205873922110590
Author(s):  
Kürşad Ramazan Zor ◽  
Gamze Yıldırım Biçer ◽  
Nesrin Tutaş Günaydın ◽  
Erkut Küçük ◽  
Uğur Yılmaz
Keyword(s):  
Choroidal Thickness ◽  
Cross Sectional Study ◽  
Control Group ◽  
Fiber Layer ◽  
Cross Sectional ◽  
Subfoveal Choroidal Thickness ◽  
Rnfl Thickness ◽  
Significant Difference ◽  
The Mean ◽  
The Right

Objectives: We aim to investigate the involvement of the choroid and retinal nerve fiber layer (RNFL) in COVID-19 patients using spectral domain optical coherence tomography. Methods: This cross-sectional study was conducted between April and June 2020. 40 patients (23 female and 17 male) with COVID-19 and 42 healthy individuals (26 female and 16 male) were included in the study. The OCT scans were performed 4 weeks after the COVID-19 diagnosis. Results: In the COVID-19 group, in the right eyes, the mean nasal choroidal thickness was 295.70 ± 7,046 μm ( p = 0.017), mean subfoveal choroidal thickness was 333.25 ± 6,353 μm ( p = 0.003), mean temporal choroidal thickness was 296.63 ± 6,324 μm ( p = 0.039), and mean RNFL was 89.23 ± 1.30 μm ( p = 0.227). In the left eyes, mean nasal choroidal thickness was 287.88 ± 9,033 μm ( p = 0.267), mean subfoveal choroidal thickness was 333.80 ± 9,457 μm ( p = 0.013), mean temporal choroidal thickness was 298.50 ± 9,158 μm ( p = 0.079), and mean RNFL was 89.48 ± 1,289 μm ( p = 0.092). Compared with the control group, the patient group had significant thickening of the choroidal thickness in all quadrants of the right eyes, and significant thickening of the subfoveal choroidal thickness in the left eyes. There was no significant difference in the RNFL thickness between groups ( p > 0.05). Conclusion: COVID-19 may cause a subclinical involvement in the choroidal layer.

167 Effect of Storage Time and Temperature of a Commercial Embryo Holding Medium on the Maturation Kinetics of Immature Bovine Oocytes

2018 ◽  
Vol 30 (1) ◽  
pp. 223
Author(s):  
O. B. Pascottini ◽  
M. Catteeuw ◽  
A. Van Soom ◽  
G. Opsomer
Keyword(s):  
Oocyte Maturation ◽  
Germinal Vesicle ◽  
Holding Time ◽  
Control Group ◽  
Nuclear Maturation ◽  
Maturation Medium ◽  
Maturation Rate ◽  
Maturation Stages ◽  
Bovine Oocytes ◽  
Kinetics Of

The effect of holding time and temperature during storage of immature bovine oocytes in a commercial embryo holding medium (EHM; Syngro® Ltd., Livingston, United Kingdom) was evaluated. Ovaries were collected at the local slaughterhouse and processed within 2 h. Cumulus-oocyte complexes (COC) were collected and allocated to groups of 60. The COC were held in 1-mL sterile glass osmometer tubes, filled to the top with the EHM to limit the amount of air. Vials were capped and covered with parafilm to ensure a tight seal and prevent leakage. Tubes were stored for 6 h at 4°C, room temperature (RT), or 38.5°C; for 10 h at 4°C and RT; and for 14 h at RT. Next, oocytes were fixed after storage in EHM (immature holding) or fixed after being held in EHM and subsequent 22-h maturation at 38.5°C in 5% CO2 in humidified air (mature holding). Maturation medium consisted of modified bicarbonate-buffered TCM-199 supplemented with gentamycin and epidermal growth factor. During all experiments, a control group was included each time. The control consisted of groups of 60 COC immediately fixed after collection or transferred to maturation medium for 22 h and subsequently fixed. Nuclear maturation of oocytes was assessed after Hoechst 33342 staining, using a 400× magnification fluorescence microscope. A total of 3043 COC were evaluated in 3 replicates. Oocytes maturation stages were classified as (1) oocytes in germinal vesicle stage, (2) oocytes in meiotic progression (diakinesis, metaphase I, or anaphase), (3) matured (telophase I or metaphase II), and (4) degenerated (degraded chromatin). Oocytes remained at the germinal vesicle stage when held in EHM (without subsequent maturation) regardless of holding time and temperature (P > 0.05). When oocytes were held for 6 h and subsequently matured (Table 1), the number of matured oocytes was significantly lower for oocytes held at 38.5°C compared with the other groups (control, RT, and 4°C). When held for 10 h, the oocyte maturation rate was similar between the control and RT groups (P > 0.05), but it was significantly lower in oocytes held at 4°C. Last, when compared with oocytes held at RT for 14 h, the maturation rate was higher in the control group (P < 0.05). To conclude, immature bovine oocytes can be successfully held in EHM at RT for up to 10 h. Storing immature oocytes in EHM can delay oocyte maturation and concomitantly synchronize maturation. Table 1.Kinetics of cumulus-oocyte complex nuclear status after storage in embryo holding medium for different times and temperatures and subsequent 22-h maturation

scholarly journals Diffusion-weighted imaging of normal appearing corticospinal tracts in patients with multiple sclerosis

2017 ◽  
Vol 31 (2) ◽  
pp. 142-146
Author(s):  
Kadihan Yalçın-Şafak ◽  
Ahmet Akça ◽  
Özlem Elibol ◽  
İrem Sarı
Keyword(s):  
Corticospinal Tract ◽  
Internal Capsule ◽  
Control Group ◽  
Corticospinal Tracts ◽  
Significant Difference ◽  
Adc Values ◽  
Study Population ◽  
The Mean ◽  
The Right ◽  
And Control

Aim To determine if there are differences in ADC values between normal appearing corticospinal tracks in patients with MS compared to ADC values in controls. Material and methods The study population comprised 62 consecutive MS patients (36 women and 26 men; mean age 36.45±8.63 years). 50 control subjects with no neurological disabilities or intracranial were included the study (32 women and 18 men; mean age 40.18±12.25 years). All ADC maps were independently evaluated by two experienced radiologists. ROI of approximately 15–18 mm2 in capsula interna and 10–12 mm2 in mesencephalon were placed bilaterally for measurement of ADC values. Three circular ROIs were placed-one each side for internal capsule-and 6 total ROIs from right and left internal capsule were averaged for each patient. Mesencephelon ADC measurements were performed similarly. Result The mean ADC values of the left internal capsule in MS patients were significiantly lower than the control group (p:0.002). No statistically significant difference was found between the MS patients and control group mean ADC values of the right internal capsule (p>0.05). The mean ADC values of the right and left mesencephalon in MS patients were significiantly lower than the control group (respectively; p:0.031, p<0.001). The mean ADC values of the left internal capsule were significiantly lower than the right internal capsule in MS patients (p<0.001). The mean ADC values of the left mesencephalon were significiantly lower than the right mesencephalon in MS patients (p<0.001). Conclusion The mean ADC values of the normal-appearing corticospinal tract in MS patients were significantly lower than the control group except for the right internal capsule.

65 OVULATION OF IMMATURE OOCYTES WITH HIGH COMPETENCE RATES

2017 ◽  
Vol 29 (1) ◽  
pp. 140
Author(s):  
A. M. Taiyeb ◽  
S. A. Muhsen-Alanssari ◽  
M. E. Kjelland ◽  
S. M. Taiyeb ◽  
A. I. Haji ◽  
...  
Keyword(s):  
Germinal Vesicle ◽  
Developmental Competence ◽  
Meiotic Maturation ◽  
Control Group ◽  
Cortical Granule ◽  
Germinal Vesicle Breakdown ◽  
Immature Oocytes ◽  
Antral Follicles ◽  
Significant Difference ◽  
High Competence

Collection of immature oocytes from antral follicles in superovulated mice is a widely established technique for retrieval of germinal vesicle (GV) and metaphase I (MI) oocytes. Investigators use their experience to select antral follicles under a microscope before puncturing the follicles. This is sometimes followed by screening of oocytes based on morphology and diameter, and usually mouse oocytes of small diameters or abnormal morphologies are excluded. Shortcomings with the current technique may include varied oocyte yields and collection of oocytes from primary and secondary follicles. Moreover, such immature oocytes were observed to have different chromatin configurations, cortical granule distributions, spindle-chromosome organizations, fertilization rates, and diameters. This study was designed to investigate the potential of ovulated immature oocytes, resultant from superovulated mice treated with an FDA approved phosphodiesterase 3A inhibitor named cilostazol (CLZ), to substitute for ovarian immature oocytes collected from antral follicles of superovulated mice. Swiss Webster mice were superovulated and gavaged with 7.5 mg of CLZ once, at the same time as hCG injection, or twice, at the same time as hCG plus 6 h post-hCG injection, to result in ovulation of MI or GV oocytes, respectively. Control ovarian GV or MI oocytes were collected from ovarian antral follicles of superovulated mice not treated with CLZ. Ten mice were used in each treatment or control group. Single or multiple administrations of CLZ resulted in mice ovulating 85.8 ± 3.9% MI oocytes or 95.2 ± 3% GV oocytes (mean ± SEM), respectively. Treated GV oocytes had significantly higher rates of advanced chromatin configuration and cortical granule distribution than did control GV oocytes. Treated GV oocytes had lower cAMP levels and higher rates of meiotic maturation, IVF, and blastocyst formation than did control GV oocytes (P < 0.0001). Treated MI oocytes had significantly higher rates of normal spindle and chromosomes aligned at the metaphase plates and offspring than did control MI oocytes. Control or treated GV oocytes were found to have greater diameters than did control or treated MI oocytes, respectively (P < 0.007), indicating that initiation of meiotic maturation is associated with reduction in oocyte diameters and utilisation of cytoplasm proteins and cofactors. Moreover, control GV oocytes were found to have greater diameters than did treated GV oocytes (P = 0.007). This may refer to the readiness of treated GV oocytes to undergo germinal vesicle breakdown and transition into the MI stage, especially treated GV oocytes had high rates of meiotic development in comparison to control GV oocytes. Diameters of GV nuclei in treated GV oocytes were smaller than those in control GV oocytes (P = 0.006), which may also indicate a germinal vesicle that had started to undergo germinal vesicle breakdown. A similar significant difference was also noted with control and treated MI oocytes. In summary, we present a novel method for retrieval of immature oocytes at different stages of meiotic maturation. Treated ovulated immature oocytes had more uniform diameters and high developmental competence than did ovarian immature oocytes. Treated ovulated immature oocytes may substitute for ovarian immature oocytes and become an additional research resource.

FOLLICULAR DEVELOPMENT, OOCYTE MATURATION AND OVULATION IN GONADOTROPHIN-TREATED PREPUBERAL CALVES

1975 ◽  
Vol 55 (2) ◽  
pp. 193-199 ◽  
Author(s):  
K. N. BEDIRIAN ◽  
R. D. BAKER
Keyword(s):  
Oocyte Maturation ◽  
Metaphase Plate ◽  
Follicular Development ◽  
Group Iv ◽  
Control Group ◽  
Group V ◽  
Group Iii ◽  
Nuclear Maturation ◽  
Group I ◽  
Gonadotrophin Releasing Hormone

Twenty pre-puberal calves, ranging from 85 to 140 kg in body weight, were divided randomly into five groups of four. Group I served as an untreated control. Group II received 1,000 IU pregnant mare’s serum gonadotrophin (PMSG) 5 days prior to slaughter. Group III received 1,000 IU human chorionic gonadotrophin (HCG) 3 days prior to slaughter. Group IV was treated with 1,000 IU PMSG, followed 5 days later with 1,000 IU HCG and slaughtered 30 h later. Group V received 200 μg of a synthetic gonadotrophin-releasing hormone (GnRH) twice daily for 5 days prior to slaughter. All injections were given intramuscularly. PMSG alone and in combination with HCG increased the number of follicles > 5 mm. Only the PMSG and HCG combination resulted in multiple ovulations (mean 12.5 CL per animal) and follicular oocyte maturation (41% in 2nd metaphase plate). Fewer than 1% of oocytes surrounded by a compact cumulus underwent nuclear maturation, whereas 81% of those with an expanded cumulus underwent nuclear maturation (P < 0.05). Alone, neither GnRH nor HCG caused any appreciable follicular development, oocyte maturation or ovulation.

scholarly journals Malposition of Unerupted Mandibular Second Premolar in Children with Palatally Displaced Canines

2009 ◽  
Vol 79 (4) ◽  
pp. 796-799 ◽  
Author(s):  
Miri Shalish ◽  
Stella Chaushu ◽  
Atalia Wasserstein
Keyword(s):  
Control Sample ◽  
Control Group ◽  
Dental Age ◽  
Genetic Etiology ◽  
Tooth Formation ◽  
Significant Difference ◽  
The Mean ◽  
Inferior Border ◽  
The Right ◽  
Age And Sex

Abstract Objective: To test the hypotheses that (1) the distal angulation of unerupted mandibular premolar (MnP2) is significantly greater in children with palatally displaced canines (PDC) than in those in a control sample; and (2) delayed tooth formation is significantly more frequent in children with both malposed MnP2 and PDC than in children with PDC only. Materials and Methods: We examined retrospectively panoramic radiographs from 43 patients with PDC who had no previous orthodontics. A control sample consisted of age- and sex-matched patients. The distal angle formed between the long axis of MnP2 and the tangent to the inferior border was measured. Dental age was evaluated using the Koch classification. Results: A significant difference was observed between the mean inclination of the right side MnP2 in the PDC group (75.4 degrees) and that of the control group (85.8 degrees). This difference was highly statistically significant (P &lt; .0001). The same evaluation was carried out for the left side, with similar results. The average dental age was found to be delayed in patients who showed both abnormalities (malposed MnP2 and PDC) compared with patients who showed the PDC anomaly only. Conclusion: Both hypotheses are retained. Statistically, PDC and MnP2 malposition are significantly associated suggesting a common genetic etiology, despite taking place on opposite jaws. While the presence of PDC or MnP2 anomaly has been associated with a delay in tooth formation, we find the presence of both anomalies to show a more profound delay. Our findings suggest a delay in tooth formation as a possible common genetic mechanism for these 2 malposition anomalies.

Determination some Immunological Aspects in Pulmonary Tuberculosis Patients in Qadisiyah Province

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Syoof Khowman Alramahy ◽  
Akram Hadi Hamza
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Statistical Difference ◽  
Positive Culture ◽  
Control Group ◽  
Tuberculosis Patients ◽  
Significant Difference ◽  
Lowenstein Jensen ◽  
The Mean ◽  
Igg Level

This study was carried out to study of some immunological aspects among the pulmonary Tuberculosis patients infected with causative agent, Mycobacterium tuberculosis. A Total of 200 sputum samples were collected from patients attending the consultant Clinic for Chest and Respiratory disease center, Diwaniya. Control group (No=15) also included. According to acid fast stain of sputum, the patients were classified as positive (No=91,45.5%) and negative (No=109,54.5, Lowenstein Jensen medium used for the cultivation of samples, on which 70% of sputum samples where positive culture for this microorganism. The grown microorganism were identified as M. tuberculosis, based on positive A.F.B, Niacin producers ,negative for catlase at 68c. The mean IgG level was l184.053±76.684 mg/100 ml in tuberculosis group compared with 1016.533 ± 44.882 mg/100ml in control group, rendering the statistical difference significant. For IgA and IgM levels, they were at mean of 315.880±38.552 mg/100 ml and 119.527±8.464 mg/100 ml in control group compared with 396.358±38.776 mg/100 ml and 134.207±11.696 mg/100 ml in patients group respectively with significant difference

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