In vivo embryo production in cows superovulated 1 or 2 days after ovum pick-up

2014 ◽  
Vol 26 (4) ◽  
pp. 527 ◽  
Author(s):  
Ricardo S. Surjus ◽  
Alexandre B. Prata ◽  
Marta Borsato ◽  
Fernanda C. S. Z. Mattos ◽  
Mariana C. Martins da Silveira ◽  
...  
Keyword(s):  
Least Squares ◽  
Corpora Lutea ◽  
Embryo Production

The present study evaluated superovulatory responses and in vivo embryo production in cows treated with FSH starting 1 or 2 days after ovum pick-up (OPU). Thirty-three non-lactating Nelore cows were subjected to aspiration of all follicles ≥3 mm for OPU. After OPU, cows were randomly divided into two groups in which the follicle superstimulatory treatments with FSH started 1 or 2 days after OPU (Groups D1 and D2, respectively). Data are presented as the least squares mean ± s.e.m. The number of follicles ≥3 mm before OPU was similar between groups (~34); however, cows in Group D2 had more follicles ≥3 mm on the first day of FSH (15.2 ± 2.3 vs 7.6 ± 1.7; P = 0.04) and a higher ratio of the number of follicles at first FSH/number of follicles before OPU (0.41 ± 0.04 vs 0.24 ± 0.02; P = 0.01). In addition, Group D2 cows had a greater superovulatory response than did cows in Group D1 (18.9 ± 2.8 vs 9.1 ± 1.9 corpora lutea, respectively; P < 0.03). However, there was no difference in the total number of recovered ova and embryos from cows in Groups D2 and D1 (5.1 ± 1.4 vs 4.9 ± 1.3, respectively; P > 0.10). Nevertheless Group D2 cows had more freezable embryos than Group D1 cows (3.2 ± 1.1 vs 1.3 ± 0.5, respectively; P < 0.05). Cows from Group D2 had a much higher proportion (P < 0.001) of follicles ≥8 mm compared with follicles ≥6 mm and <8 mm at the time of the last treatment with FSH. In conclusion, to obtain a greater production of viable embryos in superovulated cows after OPU, it is recommended to wait at least 2 days before starting FSH treatment.

scholarly journals Effect of pFSH dose reduction on in vivo embryo production in Dorper ewes

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4215
Author(s):  
João Bosco Loiola Filho ◽  
Alane Pains Oliveira do Monte ◽  
Thais Thatiane Dos Santos Souza ◽  
Mayara De Souza Miranda ◽  
Lívia Correia Magalhães ◽  
...  
Keyword(s):  
Dose Reduction ◽  
Fertilization Rate ◽  
Corpora Lutea ◽  
Device Removal ◽  
Embryo Production ◽  
Chi Square ◽  
Estrus Detection ◽  
Chi Square Test ◽  
Intravaginal Device

To evaluate the effect of pFSH dose on the in vivo embryo production of Dorper ewes in the semi-arid northeast of Brazil, 40 sheep females were distributed into two groups of 20 animals that received intravaginal CIDR for 14 days, and two days before device removal, they received one of the following treatments: in the FSH200 group, the ewes received 200 mg of pFSH; and in the FSH128 group, the ewes received a total of 128 mg in decreasing doses every 12 h. Beginning 12 h after the conclusion of the treatments, estrus detection was performed every four hours using two Dorper rams of proven fertility. The ewes were mated at estrus onset and 24 hours later. Seven days after intravaginal device removal, the superovulatory response was evaluated, and embryo collection was performed using the laparotomy method. The recovered flushings were subjected to embryo searches under a stereomicroscope and classified according to their qualities. Analyses of variance (ANOVAs) and LSD tests were used to compare the different parameters. The data expressed as percentages were analysed by chi-square test. The ovulation rate was higher in the FSH200 group, which had 16.3 ± 0.3 corpora lutea (CL), than in the FSH128 group, which had 11.3 ± 0.3 CL (P<0.05). However, higher fertilization rate (83.6% vs. 62.4%) and higher transferable (86.0% vs. 71.6%) and freezable (67.9% vs. 40.8%) embryo rates were observed in the FSH 128 group compared with the group that received 200 mg. Furthermore, no significant differences in the remaining parameters were observed between the experimental groups (P>0.05), demonstrating that pFSH dose reduction promoted a greater production of freezable and transferable embryos in Dorper ewes subjected to MOET.

scholarly journals Effect of pFSH dose reduction on in vivo embryo production in Dorper ewes

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4215 ◽  
Author(s):  
João Bosco Loiola Filho ◽  
Alane Pains Oliveira do Monte ◽  
Thais Thatiane Dos Santos Souza ◽  
Mayara De Souza Miranda ◽  
Lívia Correia Magalhães ◽  
...  
Keyword(s):  
Dose Reduction ◽  
Fertilization Rate ◽  
Corpora Lutea ◽  
Device Removal ◽  
Embryo Production ◽  
Chi Square ◽  
Estrus Detection ◽  
Chi Square Test ◽  
Intravaginal Device

<p>To evaluate the effect of pFSH dose on the <em>in vivo </em>embryo production of Dorper ewes in the semi-arid northeast of Brazil, 40 sheep females were distributed into two groups of 20 animals that received intravaginal CIDR for 14 days, and two days before device removal, they received one of the following treatments: in the FSH200 group, the ewes received 200 mg of pFSH; and in the FSH128 group, the ewes received a total of 128 mg in decreasing doses every 12 h. Beginning 12 h after the conclusion of the treatments, estrus detection was performed every four hours using two Dorper rams of proven fertility. The ewes were mated at estrus onset and 24 hours later. Seven days after intravaginal device removal, the superovulatory response was evaluated, and embryo collection was performed using the laparotomy method. The recovered flushings were subjected to embryo searches under a stereomicroscope and classified according to their qualities. Analyses of variance (ANOVAs) and LSD tests were used to compare the different parameters. The data expressed as percentages were analysed by chi-square test. The ovulation rate was higher in the FSH200 group, which had 16.3 ± 0.3 corpora lutea (CL), than in the FSH128 group, which had 11.3 ± 0.3 CL (P&lt;0.05). However, higher fertilization rate (83.6% vs. 62.4%) and higher transferable (86.0% vs. 71.6%) and freezable (67.9% vs. 40.8%) embryo rates were observed in the FSH 128 group compared with the group that received 200 mg. Furthermore, no significant differences in the remaining parameters were observed between the experimental groups (P&gt;0.05), demonstrating that pFSH dose reduction promoted a greater production of freezable and transferable embryos in Dorper ewes subjected to MOET.</p>

scholarly journals GnRH or estradiol benzoate combination with CIDR improves in-vivo embryo production in bovines (Bos indicus and Bos taurus) under subtropics

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12077
Author(s):  
Khalid Mahmood ◽  
Muhammad Zahid Tahir ◽  
Mahboob Ahmad Butt ◽  
Shazia Mansoor Qureshi ◽  
Amjad Riaz
Keyword(s):  
Bos Taurus ◽  
Bos Indicus ◽  
Estradiol Benzoate ◽  
Limiting Factors ◽  
Control Group ◽  
Corpora Lutea ◽  
Embryo Production ◽  
Varied Response ◽  
Donor Cows

Multiple Ovulation and Embryo Transfer (MOET) technology is a potential technique to upgrade livestock species’ genetics. The varied response to super-stimulatory treatments remains one of the limiting factors to this technology’s widespread use. The present study was aimed to improve the superovulation response and in-vivo embryo production by using controlled internal drug release (CIDR)-GnRH or CIDR-EB (Estradiol Benzoate) along with conventional superovulation protocol in Holstein Frisian (HF): Bos taurus; n = 42) and Crossbred (XB: Cholistani (Bos indicus) × HF; n = 28) cows. In the CIDR-GnRH/CIDR-EB treatment, CIDR was implanted in the cows after confirming the presence of a corpus luteum (CL) on the 8th day after estrus. 2 ml GnRH (Lecirelin acetate 0.0262 mg/ml) or 2 mg EB was also administered in CIDR-GnRH/CIDR-EB groups, respectively. Both groups were given super-stimulatory treatment from the 11th day after estrus (FSH in tapering doses twice a day for four consecutive days). On day 13, two doses of 2 ml prostaglandin (75 µg/ml of dextrorotatory cloprostenol) were administered (am: pm), and CIDR was removed the following day. Two artificial inseminations (AI) of the cows were performed (12 h apart) on the 15th day. No CIDR and GnRH/E.B were given in the control group, but the remaining superovulation protocol was the same. Later on, seven days after the first AI, non-surgical embryo flushing was done. The transferable embryos produced from three different superovulation protocols were then transferred into the recipient cows (n = 90) for determining their fertility. Statistical analysis revealed that the number of super-estrus follicles (SEF), multiple corpora lutea (MCL), ovulation/fertilization percentage, fertilized structures recovered (FSR), and transferable embryos (TEs) remained significantly higher (p < 0.05), and days taken for return to estrus (RTE) after embryo collection remained significantly lower (p < 0.05) in CIDR-GnRH (n = 18) and CIDR-EB (n = 15) groups as compared to the control (n = 37). The comparison between XB and HF cows revealed that the TEs production in CIDR-GnRH (XB = 5 vs HF = 13) and CIDR-EB (XB = 6 vs HF = 9) based superovulation protocols were 11.60  ±  4.08 vs 04.31  ±  0.98 and 09.33  ±  1.78 vs 05.22  ±  1.36, respectively. TEs production in XB cows (n = 5) of the CIDR-GnRH group was significantly higher (11.60  ±  4.08) than other groups. On the other hand, the days taken for RTE after embryo collection remained significantly lower (p < 0.05) in HF cows of treatment groups. However, the fertility of TEs was neither affected significantly (p > 0.05) by the superovulation protocol used nor by breed differences among donor cows. In conclusion, using CIDR-GnRH or CIDR-EB along with conventional superovulation protocol may enhance the efficiency of MOET programs in cattle. Furthermore, XB donor cows demonstrated a better performance than HF donor cows under subtropical conditions.

240 EFFECTS OF REPEATED SUPEROVULATION ON EMBRYO YIELDS IN SOME TURKISH NATIVE GOAT BREEDS

2013 ◽  
Vol 25 (1) ◽  
pp. 268
Author(s):  
M. Kaymaz ◽  
A. R. Agaoglu ◽  
K. Karakas ◽  
I. Pir Yagci ◽  
O. Korkmaz Agaoglu ◽  
...  
Keyword(s):  
Prostaglandin F2α ◽  
Repeated Administration ◽  
Corpora Lutea ◽  
Embryo Production ◽  
Recovery Rates ◽  
Embryo Recovery ◽  
The Third ◽  
Friedman's Test ◽  
Negative Effect

The Angora, Kilis, Honamli, Hair, and Norduz are native goat breeds in Turkey and are currently in danger of extinction. This study aimed to assess the efficacy of the repeated administration of a superovulatory (SOV) protocol for in vivo embryo production in these breeds. A total of 14 Angora, 15 Kilis, 10 Honamli, 10 Hair, and 9 Norduz goats were used in this work. The synchronization procedure was started on Day 5 after visible oestrus by using controlled internal drug release dispensers (CIDR®) for 11 days. Administration of FSH (Folltropin®) began on Day 9 (twice daily) and continued for 3 days (total dose: 200 mg; 50 mg × 2.30 mg × 2.20 mg × 2). A dose of prostaglandin F2α (1.6 mg; Dalmazin®) was injected together with first FSH injection. Gonadotropin-releasing hormone (Receptal®: 100 µg) was injected 6 h before mating. All goats in oestrus were naturally mated twice a day for 3 days. Ovarian examination (number of corpora lutea) and embryo recovery were performed by laparotomy on Day 6 after CIDR® withdrawal. Each uterine horn was flushed, and the embryos were recovered and counted. To avoid intra-abdominal adhesions, a 2.5% heparin solution was used during flushing. The SOV procedure was repeated once per year during the breeding season (2009 to 2011). Fertilization and recovery rates were calculated. Differences in the SOV response and embryo yields were evaluated by Friedman’s test. In Hair goats, the number of corpora lutea decreased significantly (P < 0.05) during the third SOV cycle (12.7 ± 6.2, 14.0 ± 9.1, and 6.8 ± 5.6, respectively, for cycles 1, 2, and 3), whereas no effect of the cycle was observed in the remainder of breeds. The number of expanded blastocysts increased considerably during the third cycle in Angora (0, 0.2 ± 0.8, and 1.4 ± 2.9, respectively, for cycles 1, 2, and 3), Kilis (0.2 ± 0.4, 0.3 ± 1.3, and 4.2 ± 5.0), and Honamli (0, 1.3 ± 1.7, and 3.6 ± 4.5) goats, whereas a significant decrease was observed in Norduz goats (2.4 ± 5.0, 1.8 ± 2.0, and 0.1 ± 0.3; P < 0.05). The mean numbers of unfertilized oocytes were found to be significantly increased in Angora (0.4 ± 1.6, 0, and 2.1 ± 4.1, respectively, for cycles 1, 2, and 3), Kilis (0, 1.3 ± 3.9, and 3.1 ± 5.2), and Honamli (0, 4.9 ± 5.2, and 4.5 ± 7.8) goats (P < 0.05). As a result, fertilization rates (%) showed a decrease in Angora (50, 100, 24.5, respectively, for cycles 1, 2, and 3) and Honamli (100, 42.5, and 56.3) goats (P < 0.05), whereas recovery rates showed no difference among the different breeds. The methodology presented in this study was found to be an efficient technique for superovulation of the Angora, Kilis, and Honamli goats. Because Hair and Norduz are relatively small breeds, the dosage of FSH might have had a negative effect on the superovulation and embryo yield. Additionally, the use of intra-abdominal washing solutions for preventing adhesions as observed in previous works (data not shown) is believed to have a positive effect on achieving high levels of efficiency in in vivo embryo production.

scholarly journals Effects of Extra-Long-Acting Recombinant Bovine FSH (bscrFSH) on Cattle Superovulation

Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 153
Author(s):  
Miguel A. Gutiérrez-Reinoso ◽  
Constanza J. Aguilera ◽  
Felipe Navarrete ◽  
Joel Cabezas ◽  
Fidel O. Castro ◽  
...  
Keyword(s):  
Embryo Quality ◽  
Ovarian Response ◽  
Superior Performance ◽  
Limiting Factors ◽  
Corpora Lutea ◽  
Sample Collection ◽  
Embryo Production ◽  
Expression Ratio ◽  
Long Acting

Over the last few years, several commercial FSH products have been developed for cattle superovulation (SOV) purposes in Multiple Ovulation and Embryo Transfer (MOET) programs. The SOV response is highly variable among individuals and remains one of the main limiting factors in obtaining a profitable number of transferable embryos. In this study, follicle stimulating hormone (FSH) from different origins was included in two SOV protocols, (a) FSH from purified pig pituitary extract (NIH-FSH-p; two doses/day, 12 h apart, four consecutive days); and (b) extra-long-acting bovine recombinant FSH (bscrFSH; a single dose/day, four consecutive days), to test the effects of bscrFSH on the ovarian response, hormone profile levels, in vivo embryo production and the pluripotency gene expression of the obtained embryos. A total of 68 healthy primiparous red Angus cows (Bos taurus) were randomly distributed into two experimental groups (n = 34 each). Blood sample collection for progesterone (P4) and cortisol (C) level determination was performed together with ultrasonographic assessment for ovarian size, follicles (FL) and corpora lutea (CL) quantification in each SOV protocol (Day 0, 4, 8, and 15). Moreover, FSH profiles were monitorised throughout both protocols (Day 0, 4, 5, 6, 7, 8, 9, 10, and 15). In vivo embryo quantity and quality (total structures, morulae, blastocysts, viable, degenerated and blocked embryos) were recorded in each SOV protocol. Finally, embryo quality in both protocols was assessed by the analysis of the expression level of crucial genes for early embryo development (OCT4, IFNt, CDX2, BCL2, and BAX). P4 and cortisol concentration peaks in both SOV protocols were obtained on Day 15 and Day 8, respectively, which were statistically different compared to the other time-points (p < 0.05). Ovarian dimensions increased from Day 0 to Day 15 irrespective of the SOV protocol considered (p < 0.05). Significant changes in CL number were observed over time till Day 15 irrespective of the SOV protocol applied (p < 0.05), being non- significantly different between SOV protocols within each time-point (p > 0.05). The number of CL was higher on Day 15 in the bscrFSH group compared to the NIH-FSH-p group (p < 0.05). The number of embryonic structures recovered was higher in the bscrFSH group (p = 0.025), probably as a result of a tendency towards a greater number of follicles developed compared to the NIH-FSH-p group. IFNt and BAX were overexpressed in embryos from the bscrFSH group (p < 0.05), with a fold change of 16 and 1.3, respectively. However, no statistical differences were detected regarding the OCT4, CDX2, BCL2, and BCL2/BAX expression ratio (p > 0.05). In conclusion, including bscrFSH in SOV protocols could be an important alternative by reducing the number of applications and offering an improved ovarian response together with better embryo quality and superior performance in embryo production compared to NIH-FSH-p SOV protocols.

Successive in vivo embryo production in Santa Inês sheep

2020 ◽  
Vol 60 (4) ◽  
pp. 497 ◽  
Author(s):  
Pedro Henrique Nicolau Pinto ◽  
Mario Felipe Alvarez Balaro ◽  
Helena Fabiana Reis de Almeida Saraiva ◽  
Viviane Lopes Brair ◽  
Vivian Angélico Pereira Alfradique ◽  
...  
Keyword(s):  
Recovery Rate ◽  
Doppler Ultrasonography ◽  
Corpora Lutea ◽  
Colour Doppler ◽  
Embryo Production ◽  
Previous Response ◽  
Embryo Recovery ◽  
Colour Doppler Ultrasonography ◽  
Santa Inês

Context In vivo embryo production, also called multiple ovulation and embryo transfer, can accelerate genetic gain, and thus improve animal production. However, there are issues limiting a wider use of this biotechnology in sheep livestock. Aims This study aimed to determine (1) whether a previous response to superovulation (SOV) can be used as a criterion to select ewes for in vivo embryo production, (2) whether the intensity of the SOV response (number of corpora lutea, CL) can affect the embryo recovery rate, and (3) whether the number of CL quantified by colour Doppler ultrasonography can be used to calculate the recovery rate. Methods Twenty-five Santa Inês ewes underwent SOV three times (SOV1, SOV2 and SOV3), with 200 mg FSH and natural mating. The number of CL after each SOV was determined by laparoscopy and by colour Doppler ultrasonography. Key results The number of CL significantly decreased (P &lt; 0.05) after SOV1 (7.5 ± 4.8) to 3.0 ± 5.0 at SOV 2 and 2.2 ± 3.5 at SOV3. Strong correlations were observed between SOV2 and SOV3 in terms of numbers of CL (r = 0.86, r2 = 0.74; P &lt; 0.0001) and viable embryos (r = 0.79, r2 = 0.63; P &lt; 00001). However, no correlations were observed between SOV1 and SOV2 or between SOV1 and SOV3. Recovery rate did not differ with the intensity of the SOV response (≤6, 7–10, &gt;10 CL) or between the methods used to quantify CL. Conclusions Ewes did not show the same pattern of response when submitted to successive FSH-based SOV. The intensity of the SOV response did not affect the recovery rate, and the number of CL estimated by colour Doppler ultrasonography can be used to calculate the recovery rate. Implications Selecting sheep embryo donors by a previous SOV response is not always feasible. The recovery rate is homogeneous and it is not affected by the intensity of the SOV response. A nonsurgical technique can be used to assess the recovery rate, improving animal welfare in MOET programs.

scholarly journals Ovarian response to P4-PGF-FSH Treatment in Suffolk sheep and P4-PGF-PMSG Synchronization in Cross-bred Ewes, for IVD and ET Protocol

2021 ◽  
Author(s):  
STEFAN CIORNEI ◽  
Dan DRUGOCIU ◽  
Liliana Margareta Ciornei ◽  
Petru ROŞCA
Keyword(s):  
Embryo Transfer ◽  
Development Program ◽  
Ovarian Response ◽  
Reproductive Activity ◽  
Corpora Lutea ◽  
Rapid Improvement ◽  
Embryo Production ◽  
Multiple Ovulation ◽  
Suffolk Sheep

Abstract BackgroundThe success of an embryo transfer protocol in sheep depends on many factors, but the choice of drugs for the desired superovulation as well as the conception rate are most essential. Reproductive activity in sheep is characterized by a seasonality influenced by several factors such as photoperiod, latitude, temperature, nutrition and breed. Reproductive seasonality and nutritional condition are the main factors that influence embryo production in sheep. In sheep, some anatomical peculiarities limit the application of traditional reproductive biotechnologies used in cattle. MethodsIn vivo embryo production is often referred to as “multiple ovulation and embryo transfer” and involves ovarian superstimulation of the donor female, insemination or mating, uterine flushing for embryo recovery, and either cryopreservation or transfer of collected embryos to recipients. A total number of 60 sheep and 3 rams were included in this study, divided into 2 groups (receptors/donors). Donor Suffolk sheep were treated for superovulation using the P4‐PGF‐FSH protocol while the cross-bred recipients’ group was synchronized with P4-PGF-PMSG. ResultsOn the first day after superovulation, all ovaries had more than 5 dominant follicles, while corpora lutea were later observed in 83.3% sheep. The recovery rate was 83.3% while 72,9% embryos were transferable. Embryos were transferred directly into recipients. Fertility after 30 days was 68.57%, lambing rate was 91.6%, and CR 62.85%. This study showed that veterinary drugs (P4, FSH, LH, PMSG, PGF) used for superovulation were capable to induce estrus and synchronize ovulation in sheep, are topical and in increasing use worldwide. ConclusionsThe aim of this study was to conclude on the effectiveness of a wider on farm in vivo embryo transfer development program in Suffolk sheep, using several veterinary hormones. The application of a multiple ovulation embryo transfer (MOET) protocol has a positive effect in the production of in vivo derived embryos in Suffolk sheep and can guarantee the success of embryo transfer activity to ewes with lower genetic merit. Our research aimed at representing a model for sheep farms for a rapid improvement of productive traits.

Comparison of plasma progesterone profiles in cyclic, pregnant, pseudopregnant and hysterectomized pigs between 8 and 27 days after oestrus

1988 ◽  
Vol 119 (1) ◽  
pp. 111-116 ◽  
Author(s):  
G. J. King ◽  
R. Rajamahendran
Keyword(s):  
Least Squares ◽  
Analysis Of Variance ◽  
The Other ◽  
Corpora Lutea ◽  
Plasma Progesterone ◽  
The Third ◽  
Secretory Potential ◽  
The Mean

ABSTRACT Plasma progesterone concentrations were compared in cyclic (n = 12), pregnant (n =12), oestradiol-induced pseudopregnant (n=12) and hysterectomized gilts (n=10) between days 8 and 27 after oestrus. The results were grouped into periods covering days 8–13, 14–20 and 21–27 and analysed by least-squares analysis of variance. Plasma progesterone concentrations were significantly (P<0·001) higher in hysterectomized compared with other groups between days 8 and 13. Progesterone concentrations declined rapidly after day 14 in cyclic females and gradually in the other groups. Throughout the third and fourth weeks the mean progesterone concentrations for hysterectomized animals were consistently higher than for pseudopregnant animals (P<0·05). The pregnant group means were below but not significantly different from the hysterectomized means in both of the last two periods. The greater progesterone concentrations in hysterectomized gilts indicated that secretion is high without any conceptus-produced or -mediated luteotrophin, and corpora lutea in cyclic, pregnant or pseudopregnant gilts may never reach full secretory potential. J. Endocr. (1988) 119, 111–116

scholarly journals Response of Bali cows on superovulation for in-vivo embryo production

2020 ◽  
Vol 465 ◽  
pp. 012046
Author(s):  
Muhammad Yusuf ◽  
Hasbi ◽  
Abd.Latief Toleng ◽  
Herry Sonjaya ◽  
Sahiruddin
Keyword(s):  
Embryo Production
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