Assessment of yellowtail kingfish (Seriola lalandi) as a surrogate host for the production of southern bluefin tuna (Thunnus maccoyii) seed via spermatogonial germ cell transplantation

2016 ◽  
Vol 28 (12) ◽  
pp. 2051 ◽  
Author(s):  
Ido Bar ◽  
Andre Smith ◽  
Erin Bubner ◽  
Goro Yoshizaki ◽  
Yutaka Takeuchi ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cell Transplantation ◽  
Type A ◽  
Bluefin Tuna ◽  
Dead End ◽  
Southern Bluefin Tuna ◽  
Surrogate Host ◽  
Yellowtail Kingfish ◽  
Seriola Lalandi ◽  
Type A Spermatogonia

Germ cell transplantation is an innovative technology for the production of interspecies surrogates, capable of facilitating easier and more economical management of large-bodied broodstock, such as the bluefin tuna. The present study explored the suitability of yellowtail kingfish (Seriola lalandi) as a surrogate host for transplanted southern bluefin tuna (Thunnus maccoyii) spermatogonial cells to produce tuna donor-derived gametes upon sexual maturity. Germ cell populations in testes of donor T. maccoyii males were described using basic histology and the molecular markers vasa and dead-end genes. The peripheral area of the testis was found to contain the highest proportions of dead-end-expressing transplantable Type A spermatogonia. T. maccoyii Type A spermatogonia-enriched preparations were transplanted into the coelomic cavity of 6–10-day-old post-hatch S. lalandi larvae. Fluorescence microscopy and polymerase chain reaction analysis detected the presence of tuna cells in the gonads of the transplanted kingfish fingerlings at 18, 28, 39 and 75 days after transplantation, indicating that the transplanted cells migrated to the genital ridge and had colonised the developing gonad. T. maccoyii germ cell-derived DNA or RNA was not detected at later stages, suggesting that the donor cells were not maintained in the hosts’ gonads.

Establishment of novel monoclonal antibodies for identification of type A spermatogonia in teleosts†

2019 ◽  
Vol 101 (2) ◽  
pp. 478-491 ◽  
Author(s):  
Makoto Hayashi ◽  
Kensuke Ichida ◽  
Sakiko Sadaie ◽  
Misako Miwa ◽  
Ryo Fujihara ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Monoclonal Antibodies ◽  
Rainbow Trout ◽  
Cell Surface ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Germ Cells ◽  
Type A ◽  
Germ Cell Transplantation ◽  
Type A Spermatogonia

AbstractWe recently established a germ cell transplantation system in salmonids. Donor germ cells transplanted into the body cavity of recipient embryos migrate toward and are incorporated into the recipient gonad, where they undergo gametogenesis. Among the various types of testicular germ cells, only type A spermatogonia (A-SG) can be incorporated into the recipient gonads. Enriching for A-SG is therefore important for improving the efficiency of germ cell transplantation. To enrich for A-SG, an antibody against a cell surface marker is a convenient and powerful approach used in mammals; however, little is known about cell surface markers for A-SG in fish. To that end, we have produced novel monoclonal antibodies (mAbs) against cell-surface molecules of rainbow trout (Oncorhynchus mykiss) A-SG. We inoculated mice with living A-SG isolated from pvasa-GFP transgenic rainbow trout using GFP-dependent flow cytometry. By fusing lymph node cells of the inoculated mice with myeloma cells, we generated 576 hybridomas. To identify hybridomas that produce mAbs capable of labeling A-SG preferentially and effectively, we screened them using cell ELISA, fluorescence microscopy, and flow cytometry. We thereby identified two mAbs that can label A-SG. By using flow cytometry with these two antibodies, we could enrich for A-SG with transplantability to recipient gonads from amongst total testicular cells. Furthermore, one of these mAbs could also label zebrafish (Danio rerio) spermatogonia. Thus, we expect these monoclonal antibodies to be powerful tools for germ cell biology and biotechnology.

The Pacific bluefin tuna (Thunnus orientalis ) dead end gene is suitable as a specific molecular marker of type A spermatogonia

2013 ◽  
Vol 80 (10) ◽  
pp. 871-880 ◽  
Author(s):  
Ryosuke Yazawa ◽  
Yutaka Takeuchi ◽  
Tetsuro Morita ◽  
Masashi Ishida ◽  
Goro Yoshizaki
Keyword(s):  
Molecular Marker ◽  
Type A ◽  
Bluefin Tuna ◽  
Pacific Bluefin Tuna ◽  
Thunnus Orientalis ◽  
Dead End ◽  
The Pacific ◽  
Type A Spermatogonia

scholarly journals The Southern Bluefin Tuna mucosal microbiome is influenced by husbandry method, net pen location, and anti-parasite treatment

2020 ◽  
Author(s):  
Jeremiah J Minich ◽  
Cecilia Power ◽  
Michaela Melanson ◽  
Rob Knight ◽  
Claire Webber ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Fish Species ◽  
Bluefin Tuna ◽  
Farmed Fish ◽  
Microbiome Composition ◽  
Southern Bluefin Tuna ◽  
Yellowtail Kingfish ◽  
Seriola Lalandi ◽  
And Function ◽  
The Impact

AbstractAquaculture is the fastest growing primary industry worldwide. Marine finfish culture in open ocean net pens, or pontoons, is one of the largest growth areas and is currently the only way to rear high value fish such as bluefin tuna. Ranching involves catching wild juveniles, stocking in floating net pens and fattening for four to eight months. Tuna experience several parasite-induced disease challenges in culture that can be mitigated by application of praziquantel (PZQ) as a therapeutic. In this study, we characterized the microbiome of ranched southern Bluefin Tuna, Thunnus maccoyii, across four anatomic sites (gill, skin, digesta, and anterior kidney) and evaluated environmental and pathological factors that influence microbiome composition, including the impact of PZQ treatment on microbiome stability. Southern bluefin tuna gill, skin, and digesta microbiome communities are unique and potentially influenced by husbandry practices, location of pontoon growout pens, and treatment with the antiparasitic PZQ. There was no significant relationship between the fish mucosal microbiome and incidence or abundance of adult blood fluke in the heart or fluke egg density in the gill. An enhanced understanding of microbiome diversity and function in high-value farmed fish species such as bluefin tuna is needed to optimize fish health and improve aquaculture yield. Comparison of the bluefin tuna microbiome to other fish species, including Seriola lalandi (yellowtail kingfish), a common farmed species from Australia, and Scomber japonicus (Pacific mackerel), a wild caught Scombrid relative of tuna, showed the two Scombrids had more similar microbial communities compared to other families. The finding that mucosal microbial communities are more similar in phylogenetically related fish species exposes an opportunity to develop mackerel as a model for tuna microbiome and parasite research.

Studies of Sl/Sld in equilibrium with +/+ mouse aggregation chimaeras. II. Effect of the steel locus on spermatogenesis

Development ◽  
1988 ◽  
Vol 102 (1) ◽  
pp. 117-126 ◽  
Author(s):  
H. Nakayama ◽  
H. Kuroda ◽  
H. Onoue ◽  
J. Fujita ◽  
Y. Nishimune ◽  
...  
Keyword(s):  
Mast Cells ◽  
Germ Cell ◽  
Germ Cells ◽  
Cross Sections ◽  
Sertoli Cells ◽  
Type A ◽  
Precursor Cells ◽  
Intrinsic Defect ◽  
Serial Sections ◽  
Type A Spermatogonia

Mutant mice of Sl/Sld genotype are deficient in melanocytes, erythrocytes, mast cells and germ cells. Deficiency of melanocytes, erythrocytes and mast cells is not attributable to an intrinsic defect in their precursor cells but to a defect in the tissue environment that is necessary for migration, proliferation and/or differentiation. We investigated the mechanism of germ cell deficiency in male Sl/Sld mice by producing aggregation chimaeras from Sl/Sld and +/+ embryos. Chimaeric mice with apparent white stripes were obtained. Two of four such chimaeras were fertile and the phenotypes of resulting progenies showed that some Sl/Sld germ cells had differentiated into functioning sperms in the testis of the chimaeras. In cross sections of the testes of chimaeras, both differentiated and nondifferentiated tubules were observed. However, the proportions of type A spermatogonia to Sertoli cells in both types of tubules were comparable to the values observed in differentiated tubules of normal +/+ mice. We reconstructed the whole length of four tubules from serial sections. Differentiated and nondifferentiated segments alternated in a single tubule. The shortest differentiated segment contained about 180 Sertoli cells and the shortest nondifferentiated segment about 150 Sertoli cells. These results suggest that Sertoli cells of either Sl/Sld or +/+ genotype make discrete patches and that differentiation of type A spermatogonia does not occur in patches of Sl/Sld Sertoli cells.

Flow-cytometric enrichment of Pacific bluefin tuna type A spermatogonia based on light-scattering properties

2017 ◽  
Vol 101 ◽  
pp. 91-98 ◽  
Author(s):  
Kensuke Ichida ◽  
Kazuyoshi Kise ◽  
Tetsuro Morita ◽  
Ryosuke Yazawa ◽  
Yutaka Takeuchi ◽  
...  
Keyword(s):  
Light Scattering ◽  
Type A ◽  
Bluefin Tuna ◽  
Pacific Bluefin Tuna ◽  
Flow Cytometric ◽  
Type A Spermatogonia

Differential effects of epidermal growth factor on the differentiation of type A spermatogonia in adult mouse cryptorchid testes in vitro

1991 ◽  
Vol 128 (3) ◽  
pp. 383-388 ◽  
Author(s):  
T. Haneji ◽  
S. S. Koide ◽  
Y. Tajima ◽  
Y. Nishimune
Keyword(s):  
Cell Differentiation ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Germ Cell ◽  
Type A ◽  
Induced Differentiation ◽  
Testicular Germ Cell ◽  
Germ Cell Differentiation ◽  
Epidermal Growth ◽  
Type A Spermatogonia

ABSTRACT The effect of epidermal growth factor (EGF) on testicular germ cell differentiation was investigated. Testicular fragments from surgically prepared cryptorchid testes of adult mice were cultured for 9 days in serum-free media containing various concentrations of EGF. Histological sections of testis were examined under a light microscope and each type of germ cell and mitotic cell in the seminiferous tubules was counted per 1000 Sertoli cells. EGF at concentrations ranging from 100 to 200 ng/ml induced differentiation of type A spermatogonia. The observed maximal stimulatory activity of EGF at a concentration of 100 ng/ml was 30% of the positive control cultures treated with calf serum. EGF at concentrations ranging from 1 to 100 ng/ml significantly inhibited the mitotic activity of FSH, FSH plus retinol, or FSH plus fetuin on type A spermatogonia and their differentiation. The number of type A spermatogonia in testes cultured with FSH, FSH plus retinol, or FSH plus fetuin decreased when EGF was added. On the other hand, EGF stimulated the differentiation of type A spermatogonia induced with fetuin but did not influence retinol-induced differentiation. It is proposed that EGF inhibits testicular germ cell differentiation by blocking the proliferation of type A spermatogonia stimulated by FSH. Journal of Endocrinology (1991) 128, 383–388

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