73 Use of embryo transfer technology to salvage the germplasm of elite dromedary camels (Camelus dromedarius) infected with brucellosis

2021 ◽  
Vol 33 (2) ◽  
pp. 144
Author(s):  
H. A. Abouhefnawy ◽  
N. A. Wani
Keyword(s):  
Embryo Transfer ◽  
Treatment Protocol ◽  
P Value ◽  
Dromedary Camels ◽  
Single Intramuscular Injection ◽  
Gonadotrophin Releasing Hormone ◽  
And Control ◽  
Experimental Group ◽  
Fertile Male ◽  
Group 1

The present study was conducted to evaluate the potential of embryo transfer technology to salvage invivo-produced embryos from elite dromedary camels infected with brucellosis without transmission of the pathogen to the recipients or offspring. Ten elite racing champions who tested positive for brucella comprised the experimental group, whereas 3 donors negative for brucella acted as the control. Each donor animal received a combination of 2500IU of equine chorionic gonadotrophin (eCG; Folligon; Intervet), given as a single intramuscular injection on Day 1 of the treatment protocol, and 400mg of pFSH (Folltropin; Bioniche), injected twice daily in declining doses of 2×80mg, 2×60mg, 2×40mg, and 2×20mg over 4 days, also beginning on Day 1. They were mated with a fertile bull on the 11th day after the start of treatment. The donors of the group 1 received a combination of tetracycline, and streptomycin on alternate days for 2 weeks before mating with a fertile male. An injection of 20µg of gonadotrophin-releasing hormone (Receptal, Intervet) was given to them immediately after mating to induce ovulation. The embryos were collected by the non-surgical method on Day 7 day after ovulation as per the guidelines published by IETS. All embryos were washed at least 3 times in holding medium supplemented with antibiotics and then transferred individually into the left uterine horn of synchronized recipients. All recipients and calves born were tested for brucellosis every 3 weeks. The data were analysed using a two-sample t-test (Minitab statistical software, Minitab Ltd.). Results are shown in Table 1. No difference was observed in the number of embryos collected per flushing and pregnancies established at Day 60. The proportion of pregnancies reaching term from the total number of embryos transferred and from the pregnancies established on Day 60 did not differ from embryos obtained from brucella-infected and control donors. All recipients tested negative for brucellosis during their gestation and until weaning. All calves born were also negative for brucellosis on birth and until weaning and handing over to clients. In conclusion, this is the first study in camels wherein we have demonstrated that invivo-produced embryos from elite dromedary females infected with brucellosis could be collected and transferred to synchronized recipients without transmitting the pathogen to the recipients or offspring. Table 1. Pregnancies established from invivo-produced embryos collected from elite dromedary camels infected with brucellosis Donor group (N) Total number of flushings No. of embryos collected per flushing (n±s.e.m.) Pregnancies on Day 60 (% mean±s.e.m.) Pregnancies reaching term (% mean±s.e.m.) from: Embryos transferred Pregnancies on Day 60 Brucella (10) 30 5.4±0.6 55.1±2.6 44.4±2.3 83.2±3.6 Control (3) 6 5.3±0.6 65.7±6.3 53.3±6.8 80.9±7.7 P-value 0.969 0.173 0.262 0.797

scholarly journals Pirzi Doll”: A Method for Increasing Children’s Knowledge and Attitude in Balanced Nutrition

2019 ◽  
Vol 8 (2S9) ◽  
pp. 165-169
Keyword(s):  
Nutrition Education ◽  
Control Group ◽  
P Value ◽  
Nutritional Education ◽  
Control Groups ◽  
School Aged Children ◽  
The Mean ◽  
Knowledge And Attitude ◽  
And Control ◽  
Experimental Group

Nutrition is one of the main problems in the world, where the number of malnourished patients reaches less than 104 million children and one third of all causes of child mortality worldwide are still caused by malnutrition. Nutrition knowledge in school-aged children is one of the factors determining patterns food consumption and nutritional status. Nutrition education in school-aged children can improve children's nutritional knowledge and play a role in food selection and eating habits. Nutrition education should start from an early age. Nutrition and health education began to be directed at kindergarten and elementary school students, since this age group has a habit of attitude that is relatively easy to be formed. This study aims to determine the effect of nutrition education by story’s telling with pirzi doll media to increase knowledge and attitude of children about balanced nutrition. This study used quasi-experimental design with two group pre and post-test design sampling was carried out with a systematic random sampling. Sample of research were children enrolled in Kindergarten IT Rabbani and IT Menara Fitrah in Ogan Ilir District. There were 37 samples on control group dan 39 samples on experimental group. The results of statistical analysis using Mann-Whitney test. The mean score of knowledge after being given a nutritional education was 26.15 ± 3.10 in the experimental group and in the control group was 26.05± 3.16 (p-value=0.817). The mean score of attitudes after being given a nutritional education was 13.69 ± 1.97 in the experimental group and in the control group was 11.24 ± 3.67 (p-value=0.002). There was no difference in mean score of knowledge between the experimental and control groups after being given a nutritional education. There was a difference in mean values of attitudes between the experimental and control groups after being given a nutritional education.

Interspecies embryo transfer in camelids: the birth of the first Bactrian camel calves (Camelus bactrianus) from dromedary camels (Camelus dromedarius)

2009 ◽  
Vol 21 (2) ◽  
pp. 333 ◽  
Author(s):  
A. Niasari-Naslaji ◽  
D. Nikjou ◽  
J. A. Skidmore ◽  
A. Moghiseh ◽  
M. Mostafaey ◽  
...  
Keyword(s):  
Endangered Species ◽  
Embryo Transfer ◽  
Single Injection ◽  
Bactrian Camel ◽  
Dromedary Camels ◽  
First Report ◽  
Camelus Bactrianus ◽  
Bactrian Camels ◽  
Mature Size ◽  
Fertile Male

Interspecies embryo transfer is a possible approach that can be used to conserve endangered species. It could provide a useful technique to preserve the Iranian and wild Bactrian camels, both of which are threatened with extinction. In the present study, one Bactrian camel was superovulated using decreasing doses of FSH (60, 40, 30, 30, 20, 20 mg, b.i.d.; Folltropin-V; Bioniche, London, ON, Canada) for 6 days, followed by a single injection of FSH (20 mg, i.m.) on Day 7. Daily ovarian ultrasonography was performed until most of the growing follicles had reached a mature size of 13–17 mm, at which time the camel was mated twice, 24 h apart, with a fertile male Bactrian camel. At the time of first mating, female camels were given 20 μg, i.v., buserelin (Receptal; Intervet, Boxmeer, The Netherlands). One day after the donor camel had been mated, the dromedary recipients (n = 8) were injected with 25 mg, i.v., porcine LH (Lutropin-V; Bioniche) to induce ovulation. Embryos were recovered on Day 8.5 after the first mating and transferred non-surgically into recipients on Day 7.5 after LH injection. Pregnancy was diagnosed 25 days after embryo transfer. Healthy Bactrian camel calves (n = 4) were born without any particular complications at the time of parturition (e.g. dystocia and neonatal diseases). The present study is the first report of the birth of Bactrian camel calves from dromedary camels, as well as the first report of interspecies embryo transfer in old world camelids.

scholarly journals The Effect of Strategy-Based Instruction on Students’ Speaking Skills atLakidende University

2014 ◽  
Vol 1 (1) ◽  
pp. 91
Author(s):  
Irawati . ◽  
Haryanto . ◽  
Syarifuddin Dollah
Keyword(s):  
Control Group ◽  
P Value ◽  
Speaking Test ◽  
Post Test ◽  
Speaking Skill ◽  
The Mean ◽  
Based Instruction ◽  
And Control ◽  
Experimental Group ◽  
Very High

This study aimed at investigating the effect of strategies-based instruction on students’ speaking skill at Lakidende University and the students’ interest in learning English through strategies-based instruction. This research employed Quasi Experimental Method, with non-equivalent control group design. This research assigned experimental group and control group (30 students for each).Speaking test was used to see the effect of strategies-based instruction on students’ speaking skill and questionnaire was used to see students’ interest in learning English through strategies-based instruction. The data obtained through the speaking test were analyzed by using descriptive statistics and inferential statistics. Meanwhile, the data from questionnaire were analyzed in percentage and descriptive statistics. The students’ speaking skill covered three components: accuracy, fluency, and comprehensibility. The findings showed that there was an improvement on the students’ speaking ability after the treatment. There was a significant difference between the results of post-test of experimental and control group, where the mean score of post-test of experimental group was 70.37, which was higher than the mean score of post-test of control group was 59.63.The value of P-value or sig. (2-tailed) shows that P-value or sig. (2-tailed) is smaller than α (0.00 < 0.05).Furthermore, the data that were collected from questionnaire showed that the students were very high interest toward strategies-based instruction. It is showed by the mean score in scale of 89.6, which was categorized as very high interest. Keywords: effect, strategy-based instruction, speaking skill

scholarly journals Video versus Traditional Teaching Strategy for Enhancing Intramuscular Injection Skills among College of Nursing Students in Kuwait: A Comparative Study

2021 ◽  
Vol 6 (3) ◽  
pp. 66-88
Author(s):  
Abeer William ◽  
Victoria Vidal ◽  
Helalia Moham
Keyword(s):  
Nursing Students ◽  
Intramuscular Injection ◽  
Demographic Data ◽  
Theory And Practice ◽  
Control Group ◽  
P Value ◽  
Unique Contribution ◽  
Pertinent Data ◽  
And Control ◽  
Experimental Group

Purpose: The study aims to assess the performance and compare the effectiveness of intramuscular injection skills of nursing students utilizing two different teaching strategies –traditional (with the use of performance checklist only) versus video (together with the performance checklist). Methodology: A quasi-experimental research design was used. The sample was divided into two groups - control group, 28 female nursing students and experimental group, 27 male nursing students. Socio-demographic data sheet and an observational checklist were used to collect pertinent data.. Analysis was performed using descriptive statistics and analytical tests, such as the independent t-test between the experimental and control group, regarding the preparation and injection of medication. P value of < 0.05 was considered to be significant.   Findings:  Most of the experimental and control group subjects are non-Kuwaitis. In medication preparation, the experimental group showed higher scores in the following steps: mixing solution; withdrawing prescribed amount of medication. The control group, on the other hand, scored higher in the following steps: removing needle cap, withdrawing into syringe amount of air equal to volume of medication to be withdrawn; carefully inserting needle into upright vial; and withdrawing prescribed amount of medication. In medication administration, the control group obtained higher scores in putting on clean gloves and applying gentle pressure at the site. The experimental group had higher score only in putting on clean gloves. A highly significant positive correlation was found between medication preparation and injection of medication and socio-demographic parameters of age and nationality (p= 0.008, p= 0.007) respectively.  There is no statistically significant correlation detected between control and experimental group in relation to injection of the medication.  Unique Contribution to Theory and Practice: The results of this study indicated that while traditional and video-assisted teaching are equally effective, there is no substitute for clinical demonstration.

scholarly journals Comparative study of efficacy of drotaverine hydrochloride and valethamate bromide with control in first stage of labour

2013 ◽  
Vol 11 (1) ◽  
pp. 38-42 ◽  
Author(s):  
P Dahal ◽  
B Banerjee ◽  
DK Uprety ◽  
BP Das ◽  
A Thakur ◽  
...  
Keyword(s):  
Side Effects ◽  
Mode Of Delivery ◽  
Active Phase ◽  
Fetal Outcome ◽  
Control Group ◽  
P Value ◽  
Drotaverine Hydrochloride ◽  
Group 2 ◽  
And Control ◽  
Group 1

Background: Labour is the most perilous journey a woman has to undertake. Painless and short labour is desired by every woman and is a constant aim for obstetrician.Objective: To analyze and compare the efficacy and safety of drotaverine and valethamate with control group on improving cervical dilatation and promoting progress of labour. Method: Three hundred demographically similar women, both primigravida and multigravida with a term pregnancy in established labour were randomly divided into three groups. One hundred women (group 1) were given injection valethamate intramuscularly,100 women (group 2) were given drotaverine and the rest 100 (group 3) were not given any drug. Comparative analysis was carried out as regards to duration of first stage of labour, mode of delivery, maternal side effects and fetal outcome. Results: The mean duration of active phase of labour in group 1, 2 and 3 was 254.29±96.621 min, 178.31±73.412 min and 346.31±123.351 min respectively. The duration of injection to end of first stage of labour in valethamate and Drotaverine group was 228.12±84.626min and 168.89±69.576min respectively (p value<0.0001). The duration of injection to delivery in Valethamate was 249.13± 88.321 min and in Drotaverine was 192.56±75.479min (p value<0.0001). There were no serious maternal and fetal adverse effects in any group but minor side effects were more common in valethamate group. Conclusion: The reduction of the duration of the first stage of labour was apparently more in drotaverine group as compared to valethamate and control group. Drotaverine was found to be safe with minimal or no adverse effect on the mother and the fetus. Health Renaissance, January-April 2013; Vol. 11 No.1; 38-42 DOI: http://dx.doi.org/10.3126/hren.v11i1.7600

101 Treatment with gonadotrophin-releasing hormone on Day 7 or 21 does not reduce pregnancy loss in dairy heifers receiving invitro-produced embryos

2020 ◽  
Vol 32 (2) ◽  
pp. 176
Author(s):  
B. J. Duran ◽  
R. V. Sala ◽  
P. L. J. Monteiro ◽  
C. Gamarra ◽  
M. Fosado ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Pregnancy Loss ◽  
Body Condition Score ◽  
Prostaglandin F2α ◽  
P Value ◽  
Releasing Hormone ◽  
Dairy Heifers ◽  
Condition Score ◽  
Pregnancy Status ◽  
Gonadotrophin Releasing Hormone

Previous research has shown that induction of an accessory corpus luteum (CL) by administration of gonadotrophin-releasing hormone (GnRH) on Day 5 increases circulating progesterone and reduces pregnancy loss between Days 33 and 60 in heifers receiving invitro-produced (IVP) embryos. Therefore, the objective of the present study was to determine whether timing of induction of an accessory CL influenced pregnancy loss in IVP recipients. Holstein heifers (n=1,658) were synchronized using a modified 5-day CIDR CO-Synch protocol. Briefly, heifers received an intravaginal progesterone device (CIDR) on Day −8, Day −3: CIDR removed and first prostaglandin F2α (PGF2α) treatment, Day −2: second PGF2α, and Day 0: GnRH (G1, 100µg of gonadorelin acetate). Oestrus expression was evaluated on Day 0 with the use of an oestrus detection device. Transfer of fresh IVP embryos was performed on Day 7±1 after G1. At the time of transfer, heifers were randomly assigned, in a 2×2 factorial design, to receive GnRH (200µg of gonadorelin acetate) or remain as Control (untreated) at two different times: Day 7 (time of embryo transfer (ET)) or Day 21. Thus, the following groups were formed: Control-Control (n=410); Control-GnRH (n=409); GnRH-Control (n=419); and GnRH-GnRH (n=420). All heifers were evaluated by transrectal ultrasonography to determine number, size, and location of CL on Days 5, 14, 21, 28, 32, and 60 and pregnancy status on Days 28, 32, and 60. Data collected from each heifer included embryo stage and quality, oestrus expression, body condition score, number of transfers, and technician. Pregnancies per embryo transfer (P/ET) and pregnancy loss data were analysed by logistic regression (SAS 9.4). Ovulation to Day 7 treatment was greater (P&lt;0.01) in GnRH treated heifers (70.0%; 585 out of 836) than in untreated Controls (2.7%; 22 of 819). Ovulation to Day 21 treatment was determined only in animals pregnant at Day 28 and was greater (P&lt;0.01) in heifers treated with GnRH (37.6%; 153 of 407) than in untreated controls (1.0%; 4 of 390). There was no effect of Day 7 treatment (P&gt;0.68) or Day 21 treatment (P&gt;0.18), nor a Day 7×Day 21 treatment interaction (P&gt;0.48) on P/ET at Day 32 or 60 (Table 1). Treatment with GnRH on Day 7 or 21 did not alter pregnancy loss between Days 32 and 60 (P&gt;0.10). Heifers with an accessory CL present at Day 32 (11.7%; 41 of 350) had similar (P=0.55) pregnancy loss compared with heifers with no accessory CL (14.4%; 54 of 375). The number of CL present on Day 32 did not affect (P=0.23) pregnancy loss; however, heifers with 3 CL (4.9%; 3 of 61) tended (P&lt;0.10) to have reduced pregnancy loss compared with heifers with 1 CL (14.4%; 54 of 375) or 2 CL (13.2%; 38 of 289). In conclusion, treatment with GnRH on Day 7 or 21 induced an accessory CL; however, it did not affect P/ET on Days 32 or 60 and pregnancy loss. Table 1.Pregnancies per embryo transfer (P/ET) and pregnancy loss in embryo recipients based on timing of treatment with gonadotrophin-releasing hormone (GnRH) Treatment P/ET Day 32% (n) P/ET Day 60% (n) Pregnancy loss% (n) Day 7 Day 21 Control Control 42.4 (410) 36.1 (410) 15.0 (174) GnRH 46.0 (409) 40.7 (409) 11.7 (188) GnRH Control 43.7 (419) 36.8 (419) 15.9 (183) P-value GnRH 43.1 (420) 38.8 (420) 10.0 (181) Day 7 treatment 0.68 0.72 0.96 Day 21 treatment 0.51 0.18 0.11 Interaction 0.48 0.66 0.71

Physical intervention to relieve pain in women with primary dysmenorrhoeaA new perspective

2020 ◽  
Vol 11 (4) ◽  
pp. 5071-5075
Author(s):  
Sujatha B ◽  
Jagatheesan Alagesan ◽  
Tinu Priya R ◽  
Sarumathi S
Keyword(s):  
Standard Deviation ◽  
Treatment Protocol ◽  
Control Group ◽  
P Value ◽  
Daily Life Activities ◽  
Primary Dysmenorrhoea ◽  
Relieve Pain ◽  
Post Test ◽  
New Perspective ◽  
Experimental Group

Dysmenorrhoea is menstruation with pain involving abdominal cramps. It can affect females daily life activities and productivity. Aim of the study is to find the effectiveness of exercise and various stretching techniques used as a treatment protocol for primary dysmenorrhoea. Forty-five students were selected based on a questionnaire. Out of this, 38 subjects were selected and divided into two groups as the control group (n=19) and the experimental group (n=19). Each group were given separate exercise protocol. The post-test value will be taken after eight weeks (2 menstrual cycles). The exercises were performed from the sixth day of the menstrual cycle. The results were determined from the statistical analysis. It has been revealed that there is a significant improvement in the experimental group than the control group. In the control group, the pre-test mean is 7.84, with the standard deviation being 1.30 and the post-test mean 5.10 with a standard deviation of 1.96. P-value in the control group was less than 0.0001. In the experimental group, the pre-test mean is 7.52, with the standard deviation being 1.54 and the post-test mean being 4.57 and a standard deviation of 2.19. P-value in the experimental group was less than 0.0001. The overall results show that there is a significant improvement in pain (VAS) in both treatment groups. The results obtained suggested that both strengthening and stretching techniques have a positive effect on the treatment of primary dysmenorrhoea. The improvement is more significant in the experimental group.

The evolution of improved and simplified superovulation protocols in cattle

2012 ◽  
Vol 24 (1) ◽  
pp. 278 ◽  
Author(s):  
Reuben J. Mapletoft ◽  
Gabriel A. Bó
Keyword(s):  
Embryo Transfer ◽  
Treatment Protocol ◽  
Bovine Embryo ◽  
Treatment Protocols ◽  
Wave Dynamics ◽  
Follicular Wave ◽  
Recent Treatment ◽  
Gonadotrophin Releasing Hormone ◽  
Animal Handling ◽  
On Farm

Superovulation protocols have improved greatly since the early days of bovine embryo transfer when purified gonadotrophins were not available, follicular wave dynamics were unknown physiological phenomena and prostaglandins were not available. Although superstimulatory protocols in cattle are normally initiated mid-cycle, elective control of follicular wave emergence and ovulation have had a great impact on the application of on-farm embryo transfer. However, the most common treatment for the synchronisation of follicular wave emergence involves the use of oestradiol, which cannot be used in many parts of the world. Therefore, the need for alternative treatments has driven recent research. An approach that has shown promise is to initiate follicle-stimulating hormone (FSH) treatments at the time of the emergence of the new follicular wave following ovulation induced by gonadotrophin-releasing hormone. Alternatively, it has been shown that it may be possible to ignore follicular wave status and, by extending the treatment protocol, induce subordinate follicles to superovulate. Finally, the short half-life of pituitary FSH necessitates twice-daily treatments, which are time-consuming, stressful and subject to error. Recent treatment protocols have permitted superstimulation with a single FSH treatment or two treatments 48 h apart, reducing the need for animal handling during gonadotrophin treatments.

Zinc Supplementation in Preterm Neonates with Late-Onset Sepsis: Is It Beneficial?

2020 ◽  
Author(s):  
Mohamed Shawky Elfarargy ◽  
Ghada Al-Ashmawy ◽  
Sally Abu-Risha ◽  
Haidy Khattab
Keyword(s):  
Late Onset ◽  
Intervention Group ◽  
Preterm Neonates ◽  
Control Group ◽  
P Value ◽  
Late Onset Sepsis ◽  
Sepsis Score ◽  
The Mean ◽  
And Control ◽  
Group 1

Objective Neonatal sepsis (NS) is a serious neonatal disease. The aim of this study was to detect the role of zinc (Zn) supplementation in preterm neonates with late-onset sepsis (LOS). Study Design A prospective randomized clinical trial study which was done at Tanta University Hospital from August 2016 to March 2018 on 180 preterm neonates with LOS. The studied neonates were divided into two groups: group 1 (90 neonates), which received Zn and antibiotics, and group 2 (90 neonates), which received antibiotics and placebo. In group 1, the neonates received 1.4 mg elemental Zn/kg/d orally for 10 days. Sepsis score, C-reactive protein (CRP), and procalcitonin (PCT) were done for both groups. Results As regards sepsis score, it showed that before beginning the treatment, there were 85 and 84 neonates who had high probable sepsis (HPS) in intervention and control groups, respectively, and this revealed nonstatistically significant difference (non-SSD) between both groups (p-value is 0.756) and after 10 days of treatment, there were 1 and 4 neonates who had HPS in intervention and control group, respectively, and this revealed SSD between both groups (p-value is 0.045*). As regards CRP and PCT, the results showed that before beginning the treatment, the mean ± standard deviation (SD) of CRP and PCT were 39.4 ± 10.1 mg/L and 5.2 + 1.8 ng/mL, respectively, in intervention group, while it was 39.6 + 9.9 mg/L and 5.1 + 1.9 ng/mL, respectively, in control group and this revealed non-SSD between both groups (p-value is 0.893 and 0.717, respectively) and after 10 days of treatment, the mean ± SD of CRP and PCT were 5.3 ± 1.8 mg/L and 0.39 ± 0.13 ng/mL, respectively, in intervention group and 6.1 + 2 mg/L and 0.61 + 0.22 ng/mL, respectively, in control group and this revealed SSD between both groups (p-value is 0.008* and 0.044*, respectively). Conclusion Zn supplementation in preterm neonates with LOS is beneficial in improving the clinical and laboratory finding. Recommendation Zn supplementation for preterm neonates with LOS. Key Points

scholarly journals THE EFFECTIVENESS OF KNOW –WANT-LEARN (KWL) STRATEGY IN TEACHING READING COMPREHENSION

2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Husnaini Husnaini
Keyword(s):  
Reading Comprehension ◽  
Alternative Hypothesis ◽  
Research Result ◽  
Control Group ◽  
P Value ◽  
Test Form ◽  
Eighth Grade Students ◽  
Quasi Experimental ◽  
And Control ◽  
Experimental Group

The objectives of the research is to find out whether or not the use of KWL strategy is more effective than non KWL strategy in improving students’ interpretive comprehension. The research applied quasi experimental design in which the researcher used two groups namely experimental group with treatment by using KWL strategy and control group non KWL strategy. The subject of this research was the eighth grade students of SMP Neg. 9 Palopo with the number of sample is 60 students, 30 students in experimental group and 30 students in control group. The researcher gave pretest before treatment to both groups and gave treatment to experimental group in six meetings and the last the researcher gave posttest. The data were collected through reading comprehension test in objective test form. The research result indicated that the use of KWL strategy is more effective than non KWL strategy in improving students’ interpretive comprehension. It was proven by the t-test of students’ posttest on this level of comprehension between experimental and control group where Probability value was lower than alpha (.000<0.05). It indicates that null hypothesis (H0) is rejected and alternative hypothesis (H1) is accepted because P-value < α.

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