18 All aboard the polar express: transferability of a cryopreservation protocol between anuran species

2022 ◽  
Vol 34 (2) ◽  
pp. 243
Author(s):  
I. Burger ◽  
L. D. Chen ◽  
D. Barber ◽  
V. Poole ◽  
D. Smith ◽  
...  
Keyword(s):  
Anuran Species ◽  
Cryopreservation Protocol

The advertisement call of Allobates niputidea (Anura: Aromobatidae)

Zootaxa ◽  
2019 ◽  
Vol 4656 (1) ◽  
pp. 196-200
Author(s):  
ANA MARÍA OSPINA-L. ◽  
DANIELA MURILLO-BEDOYA ◽  
DANIELA GARCÍA-COBOS ◽  
ZUANIA COLÓN-PIÑEIRO ◽  
ANDRÉS ACOSTA-GALVIS
Keyword(s):  
Acoustic Signal ◽  
Phylogenetic Relationships ◽  
Advertisement Call ◽  
Evolutionary Processes ◽  
Behavioral Interactions ◽  
Diagnostic Characters ◽  
Anuran Species

In anurans, acoustic signal traits are useful for understanding patterns of evolutionary processes, behavioral interactions, and providing diagnostic characters for inferring phylogenetic relationships and delimiting species (Cocroft & Ryan 1995). The advertisement call, which is the vocalization emitted to attract females or segregate conspecific males, is the most conspicuous and studied acoustic signal (Toledo et. al. 2014). However, it remains unknown for many anuran species (Köhler et al. 2017; Guerra et al. 2018). 

scholarly journals Effects of Cryoprotective Medium Composition, Dilution Ratio, and Freezing Rates on Spotted Halibut (Verasper variegatus) Sperm Cryopreservation

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2153
Author(s):  
Irfan Zidni ◽  
Yun Ho Lee ◽  
Jung Yeol Park ◽  
Hyo Bin Lee ◽  
Jun Wook Hur ◽  
...  
Keyword(s):  
Previous Experiment ◽  
Activity Index ◽  
Medium Composition ◽  
Freezing Rate ◽  
Dilution Ratio ◽  
Sperm Cryopreservation ◽  
Ringer’S Solution ◽  
Potential Market ◽  
Spotted Halibut ◽  
Cryopreservation Protocol

The spotted halibut is species that has a high potential market value in Korea, but the supply of seed is unstable because of the limited milt production of males. The objective of this research was to explore different aspects, such as CPAs, diluents, dilution ratio, and freezing rates, to develop an optimal sperm cryopreservation. The parameters assessed were movable sperm ratio, sperm activity index, survival rate, and DNA damage. The CPAs tested in this research were propylene glycol, dimethyl sulfoxide (DMSO), methanol, ethylene glycol, and glycerol. Different diluents, including 300 mM sucrose, 300 mM glucose, Stain’s solution, and Ringer’s solution, were investigated. The previous experiment showed that the optimal CPA for cryopreservation was DMSO with a concentration of 15% with 300 mM as diluent. To determine the effect of the dilution ratio, sperm was diluted to 1:1, 1:2, 1:10, 1:100, and 1:1000 with 300 mM sucrose containing DMSO at a final concentration of 15%. Lastly, the optimal freezing rate of the sperm was evaluated with four different freezing rates (−1, −5, −10, and −20 °C/min). Post-thaw sperm motility was higher with a dilution ratio lower than 1:2, and the freezing rate was less than −5 °C/min. In conclusion, these findings represent the development of a cryopreservation protocol for spotted halibut.

Larval diets of two anuran species, Pelodytes punctatus and Bufo bufo, in SW Spain

1989 ◽  
Vol 10 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Carmen Diaz-Paniagua
Keyword(s):  
Water Column ◽  
Bufo Bufo ◽  
Sw Spain ◽  
Anuran Species

AbstractDiets of both species studied were basically composed of algae and detritus. For Pelodytes punctatus, other food types exhibited minor contributions to the diet. For Bufo bufo, phanerogams reached considerable proportions too and the frequency of animals may be considered noteworthy if related to other species in the area. Morphologically they seem to be both conditioned to bottom dwelling rather than to the use of water column.

Investigating the species-specific association between anuran calling phenology and air temperature

2021 ◽  
Author(s):  
Samantha Wong
Keyword(s):  
Climate Change ◽  
Air Temperature ◽  
Management Strategies ◽  
Temporal Trends ◽  
Interspecific Variation ◽  
Northern Ontario ◽  
Anuran Species ◽  
Calling Behaviour ◽  
Survival And Development ◽  
Species Specific

Climate change has been associated in phenological shifts for a variety of taxa. Amphibians, specifically the order Anura (frogs and toads), are considered particularly vulnerable due to their sensitivity to anthropogenic and environmental change. Previous research has documented shifts in the timing of anuran breeding that can be attributed, in part, to climate change, with potential implications for reproduction, survival, and development. This study aims to investigate how air temperature is associated with anuran calling phenology. I will examine the temporal trends in spring and summer air temperature in a lake in northern Ontario, Canada. and quantify seasonal patterns of calling anuran species using acoustic monitoring over a four-month period. I predict that there will be interspecific variation in peak calling associated with air temperature. Additionally, I expect to find asymmetrical association between air temperature and anuran species’ calling behaviour – wherein prolonged breeding species will have a larger optimal temperature range for calling compared to explosive breeding species. The findings of this research will aid in future conservation and provide insight for management strategies of anurans in Canada in response to anticipated climate warming.

scholarly journals Composition and diversity of anurans in the Restinga of the Conde municipality, northern coast of the state of Bahia, northeastern Brazil

2016 ◽  
Vol 16 (3) ◽  
Author(s):  
Felipe Augusto Trindade Gondim-Silva ◽  
Alessandra Rodrigues Santos Andrade ◽  
Rafael Oliveira Abreu ◽  
Jamile Santos Nascimento ◽  
Geovane Paixão Corrêa ◽  
...  
Keyword(s):  
Species Richness ◽  
Species Diversity ◽  
Species Composition ◽  
Rainy Season ◽  
Dominant Species ◽  
The State ◽  
Northeastern Brazil ◽  
Freshwater Wetland ◽  
Northern Coast ◽  
Anuran Species

Abstract The Conde municipality is located in the northern coast of the state of Bahia (NC), northeastern Brazil, and is part of the Atlantic Tropical domain. The anuran fauna of the northern portion of the NC is still poorly known if compared to the southern portion. The Restinga is one of the predominant environments of the coastal plains of the NC and it is characterized essentially by presenting sandy soil covered by herbaceous and shrubby vegetation. The objective of this study was to determine the anuran species composition and diversity for the Restinga of the Conde municipality. Sampling was carried out at night by active search over four periods of five consecutive days each, two over the 'main rainy season' and two in a 'lesser rainy season', using 14 sample units (SUs) and five extra sample plots (EPs). We calculated dominance and species diversity using the Berger-Parker and Shannon-Wiener H' indices, respectively. We used accumulation curves and the Jackknife 1 estimator to estimate anuran species richness, considering only the data obtained from the SUs. We recorded 713 anuran specimens distributed within 33 species, 13 genera and five families (Bufonidae, Craugastoridae, Hylidae, Leptodactylidae and Microhylidae). The Hylidae and Leptodactylidae families had the highest species richness. Considering only the SUs (Jackknife 1 estimator in brackets), we recorded 28 species in the study area (33.9 ± 2.3), 13 in Shrubby Vegetation Zones - SVZ (20.8 ± 2.9) and 25 in Freshwater Wetland Zones - FWZ (28.9 ± 1.9). The abundance and species diversity of the FWZ (n = 638 specimens; H'= 2.4) were higher than those recorded for the SVZ (n = 52 specimens; H' = 1.9). The SVZ and FWZ showed distinct dominant species, wherein Pristimantis paulodutrai was the dominant species in SVZ and Scinax fuscomarginatus in FWZ. The Restinga of the Conde municipality stands out as the one with the highest anuran species richness already recorded considering only SVZ and FWZ. Moreover, its anuran species composition represented 55% of the anuran species known for the NC and included taxa common to three different morphoclimatic domains (Tropical Atlantic, Cerrado and Caatinga).

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