87 Kinematic and morphological properties of Large White boar sperm under induced oxidative stress

2022 ◽  
Vol 34 (2) ◽  
pp. 280
Author(s):  
M. R. Ledwaba ◽  
M. L. Mphaphathi ◽  
M. A. Thema ◽  
C. M. Pilane ◽  
T. L. Nedambale
Keyword(s):  
Oxidative Stress ◽  
Morphological Properties ◽  
Large White ◽  
Boar Sperm

Involvement of oxidative stress in municipal landfill leachate-induced toxicity in boar sperm

2013 ◽  
Vol 36 (3) ◽  
pp. 972-978 ◽  
Author(s):  
Isaac A. Adedara ◽  
Oluwatosin O. Oyebiyi ◽  
Tajudeen A. Lawal ◽  
Adebayo A. Adesina ◽  
Ebenezer O. Farombi
Keyword(s):  
Oxidative Stress ◽  
Landfill Leachate ◽  
Municipal Landfill ◽  
Boar Sperm

scholarly journals 79 EFFECT OF PRE-FREEZE ADDITION OF PLATELET-ACTIVATING FACTOR AND PLATELET-ACTIVATING FACTOR:ACETYLHYDROLASE ON THE POST-THAW INTEGRITY OF FROZEN - THAWED BOAR SPERM

2005 ◽  
Vol 17 (2) ◽  
pp. 189
Author(s):  
R. Bathgate ◽  
B.M. Eriksson ◽  
W.M.C. Maxwell ◽  
G. Evans
Keyword(s):  
Platelet Activating Factor ◽  
Egg Yolk ◽  
Large White ◽  
Boar Sperm ◽  
Thawing Process ◽  
Potential Benefits ◽  
Boar Semen ◽  
Acrosome Integrity ◽  
And Control

The use of frozen-thawed boar sperm is not widespread, owing to reduced fertility rates and high cost per dose (Eriksson et al. 2004 Proc. Aust. Assoc. Pig Vet., 61–69). Improvements in post-thaw sperm survival are required for commercialization. Platelet-activating factor (PAF) is a phospholipid involved in regulating sperm function. PAF:acetylhydrolase (PAF:AH) regulates PAF activity by conversion to its inactive isoform. Both occur naturally in boar semen (Kordan et al. 2003 Pol. J. Vet. Sci. 6, 55–60). Removal of PAF and PAF:AH along with seminal plasma during the cryopreservation process may inhibit the ability of sperm to withstand the freeze-thawing process. The aim of this study was to assess the effect of PAF and PAF:AH, added to boar semen pre-freeze, on the post-thaw motility and acrosome integrity of sperm. The sperm rich fraction was collected from a mature Large White × Landrace boar, diluted with Androhep (1:2, semen:Androhep; Minitube, Verona, WI, USA), cooled to 17°C over 2 h, and then centrifuged (10 min, 800g). The sperm pellet was resuspended in cooling extender (11% (w/v) lactose solution with 20% (v/v) egg yolk; control), cooling extender plus 100 ng/mL PAF (PAF), or cooling extender plus 0.4% (v/v) PAF:AH (Pafase; ICOS Corporation, Seattle, Washington, USA), and cooled to 5°C over 2.5 h. Sperm were further diluted with cooling extender plus 9% (v/v) glycerol and 1.5% (v/v) Equex STM (freezing extender), loaded into 0.5-mL straws, and frozen. Straws were thawed (20 s, 42°C) and the motility and acrosome integrity (FITC-PNA; Mortimer etal. 1990 Hum. Reprod. 5, 99–103) assessed at 0, 3, and 6 h post-thaw after incubation at 37°C. Data from three replicates were analyzed by ANOVA and a Tukey test applied where significant differences were found. Post-thaw motility (0 and 3 h) was higher for PAF (60.0 ± 0.0% and 25.0 ± 2.9%) than for control (41.7 ± 1.7% and 10.0 ± 2.9%; P < 0.05), but was similar for Pafase (41.7 ± 1.7% and 16.7 ± 1.7%; P > 0.05). By 6 h post-thaw, motility was similar for PAF (1.7 ± 1.7%), Pafase (6.7 ± 6.8%), and control (1.7 ± 1.7%, all respectively; P > 0.05). Acrosome integrity was higher at 0, 3 and 6 h post-thaw for Pafase (55.7 ± 3.2%, 45.7 ± 3.7% and 23.0 ± 3.1%) than for control (42.7 ± 1.5%, 25.7 ± 5.7% and 12.3 ± 2.7%) and PAF (33.0 ± 3.7%, 26.3 ± 2.2% and 11.7 ± 0.3%, all respectively; P < 0.05), but was similar between control and PAF (P > 0.05). Supplementation of cooling extender with 100 ng/mL PAF increased initial post-thaw motility, but this benefit was lost after 6 h post-thaw. Pafase in the cooling extender improved the proportion of intact acrosomes, even after 6 h post-thaw. In vitro studies investigating the interaction between Pafase-treated frozen-thawed sperm and oviducal epithelial cells would be of interest to further establish the potential benefits of pre-freeze addition of Pafase on the fertilizing potential of frozen-thawed boar sperm.

scholarly journals Changes of sperm morphology in Duroc, Landrace and Large White boars depending on the ambient temperature during the year

2021 ◽  
Author(s):  
V Kamanova ◽  
P Nevrkla ◽  
Z Hadas ◽  
J Lujka ◽  
R Filipcik
Keyword(s):  
Czech Republic ◽  
Ambient Temperature ◽  
Sperm Morphology ◽  
Sperm Quality ◽  
Large White ◽  
Boar Sperm

Utilisation of artificial insemination has led to the increased role of male animals. They significantly contribute to the efficiency of productivity; therefore, sperm quality has been emphasised. The aim of this study was to analyse changes in the boar sperm morphology during the year in forty-two Duroc, Landrace and Large White boars in the Czech Republic. For each boar, a spermiogram was evaluated every month in 2018. The number of morphologically abnormal spermatozoa was recorded every month to evaluate the morphology. The abnormalities were categorised as abnormalities of the head, abnormalities of the acrosome, proximal cytoplasmic droplets and abnormalities of the tail. Throughout the study, the temperature in the stable was monitored. Changes in the sperm morphology were recorded in all breeds during the year, the changes were different for each breed. In the Landrace boars, the highest concentrations of morphologically abnormal spermatozoa were observed in July and August (P &lt; 0.05). In this period, the concentration of the tail defects increased (P &lt; 0.05), while, in October, a higher concentration (P &lt; 0.05) of spermatozoa with defects of the acrosome was recorded in the Landrace boars. In the Duroc and Large White boars, the highest morphologically abnormal spermatozoa values were observed in September and October (P &lt; 0.05). In the Duroc boars, the increase in the morphologically abnormal spermatozoa in this period was mainly caused by an increased occurrence of proximal cytoplasmic droplets (P &lt; 0.05). On the contrary, in the Large White boars, the increase was related to a higher incidence of tail defects (P &lt; 0.05). These results show that changes in the sperm morphology are influenced by the ambient temperature and the breed.

119 OXIDATIVE STRESS DURING THE CRYOPRESERVATION OF BOAR SPERMATOZOA

2007 ◽  
Vol 19 (1) ◽  
pp. 177 ◽  
Author(s):  
M. Hernandez ◽  
J. M. Vazquez ◽  
E. A. Martinez ◽  
J. Roca
Keyword(s):  
Oxidative Stress ◽  
Egg Yolk ◽  
Ros Production ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide ◽  
Intracellular Ros ◽  
Boar Sperm ◽  
Ros Formation ◽  
Boar Spermatozoa

The cryopreservation procedure causes dramatic changes in boar sperm survival but it is yet unclear where and how the process affects spermatozoa. Cryopreservation damage appears partly associated with oxidative stress and reactive oxygen species (ROS) generation. The present study evaluates the effect that various steps of a conventional cycle of cryopreservation have on the intracellular production of ROS by boar spermatozoa (spz). Sperm-rich fractions collected from 2 mature boars (3 ejaculates per boar), cooled to 17�C, and kept for 16 h were cryopreserved following a standard freeze–thaw process with 0.5-mL plastic straws. The production of ROS was recorded in 5 steps of the cryopreservation process. These steps were as follows: step (1) after collection, when the fresh semen was extended (1:1, v/v) in Beltsville Thawing Solution (BTS, 205 mM glucose, 20.39 mM NaCl, 5.4 mM KCl, 15.01 mM NaHCO3, and 3.35 mM EDTA); step (2) after cooling and storage for 16 h at 17�C; step (3) after centrifugation (2400g for 3 min) and re-extension of the pellet with lactose-egg yolk extender; step (4) at 5�C, after the addition of lactose-egg yolk-glycerol-Equex Stem Paste to 1 � 109 spz mL; and step (5) immediately after thawing at 37�C for 20 s. For the ROS measurement, all samples were re-extended in BTS (3 � 106 spz mL-1) and incubated without (basal ROS level) or with ROS inducers (1 mM tert-butyl hydroperoxide) for 120 min at 37�C and 5% CO2. Cells were simultaneously stained with 22,72-dichlorodihydrofluorescein diacetate (1 �M) to estimate the production of ROS, and propidium iodide (12 �M) to exclude dead sperm from the analysis. Samples were evaluated at 30 min and 120 min by flow cytometry (Coulter Epics XL; Coulter Corporation, Miami, FL, USA); further analyses of the parameters were done by FCSExpress software (DeNovo Software, Thornhill, Ontario, Canada). ROS production was expressed as the mean of the green intensity fluorescence units of the viable sperm population. Data from 3 replicates were analyzed as a split plot design using a mixed model ANOVA including cryopreservation step, boar, and incubation time as fixed effects and replicate as random effect. Results indicated that the basal ROS formation remained relatively low and constant (P = 0.95) through the cryopreservation process, without differences between boars (P = 0.559), although with a significant increase after 120 min of incubation (P &lt; 0.001). However, the exposure to tert-butyl hydroperoxide significantly increased the intracellular ROS formation in all of the steps (P &lt; 0.001), showing significant differences between them, and being especially raised at steps 3 and 4. In conclusion, the present study confirms that the basal intracellular ROS production during cryopreservation of boar sperm is low. Nevertheless, the susceptibility of those spermatozoa to external stresses vary through the cryopreservation process, especially after centrifugation and later extension at 17�C and after the slow cooling at 5�C. This work was supported by CICYT (AGF2005-00706), Madrid, Spain

scholarly journals Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1549
Author(s):  
Chengwen Feng ◽  
Zhendong Zhu ◽  
Wenjing Bai ◽  
Rongnan Li ◽  
Yi Zheng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sperm Quality ◽  
Redox Homeostasis ◽  
Membrane Integrity ◽  
Underlying Mechanism ◽  
Proline Dehydrogenase ◽  
Progressive Motility ◽  
Boar Sperm ◽  
Amine Structure ◽  
Boar Semen

Proline was reported to improve sperm quality in rams, stallions, cynomolgus monkeys, donkeys, and canines during cryopreservation. However, the underlying mechanism remains unclear. The aim of this study was to investigate the effect of proline on boar semen during liquid storage at 17 °C and explore the underlying mechanism. Freshly ejaculated boar semen was supplemented with different concentrations of proline (0, 25, 50, 75, 100, 125 mM) and stored at 17 °C for nine days. Sperm motility patterns, membrane integrity, ATP (adenosine triphosphate), reactive oxygen species (ROS), and GSH (glutathione) levels, and the activities of catalase (CAT) and superoxide dismutase (SOD) were evaluated after storage for up to five days. It was observed that boar sperm quality gradually decreased with the extension of storage time, while the ROS levels increased. Addition of 75 mM proline not only significantly improved sperm membrane integrity, motility, and ATP levels but also maintained the redox homeostasis via increasing the GSH levels and activities of CAT and SOD. When hydrogen peroxide (H2O2) was used to induce oxidative stress, addition of proline significantly improved sperm quality and reduced ROS levels. Moreover, addition of proline also improved sperm quality during the rapid cooling process. Notably, addition of DL-PCA (DL-pipecolinic acid) rescued the reduction of progressive motility and total motility caused by H2O2, and THFA (tetrahydro-2-furoic acid) failed to provide protection. Furthermore, addition of proline at 75 mM increased the activity of proline dehydrogenase (PRODH) and attenuated the H2O2-induced reduction in progressive motility. These data demonstrate that proline protects sperm against oxidative stress through the secondary amine structure and proline dehydrogenase-mediated metabolism.

scholarly journals 304 PRODUCTION OF PORCINE EMBRYOS OF A PREDETERMINED SEX AFTER IN VITRO FERTILIZATION OF IN VITRO-MATURED OOCYTES WITH SEX-SORTED FROZEN-THAWED BOAR SPERM

2005 ◽  
Vol 17 (2) ◽  
pp. 303 ◽  
Author(s):  
R. Bathgate ◽  
K.M. Morton ◽  
B.M. Eriksson ◽  
D. Rath ◽  
B. Seig ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Restriction Analysis ◽  
Motile Sperm ◽  
Large White ◽  
Vitro Fertilization ◽  
Boar Sperm ◽  
Fort Collins ◽  
Cattle And Sheep

Pre-sexed embryos and offspring have been produced after IVF and embryo transfer (ET) with sex-sorted frozen-thawed sperm in cattle and sheep (Maxwell et al. 2004 Anim. Reprod. Sci. 82–83, 79–95). The aims of this study were to demonstrate that sex-sorted frozen-thawed boar sperm could be incorporated into pig IVF for the production of embryos of a predetermined sex and that these embryos could be successfully nonsurgically transferred. Ovaries were collected from abattoir slaughtered gilts (n = 138) and selected COCs were matured in vitro (Long et al. 1999 Theriogenology 51, 1375–1390). Sperm were collected from a mature boar and diluted with Androhep (1:3, semen:Androhep; Minitube, Verona, WI, USA), stained with H33342, and separated into X and Y sperm using a SX MoFlo (Cytomation, Inc., Fort Collins, CO, USA). Sex-sorted sperm were cryopreserved in 0.5 mL straws using the Westendorf protocol modified for sorted sperm (Bathgate, unpublished). Thawed sperm (Y sperm only) were prepared for IVF by centrifugation (300g, 10 min) through a Porcipure gradient (Nidacon Int. AB, Gothenburg, Sweden), and washed (centrifugation 300g, 10 min) in mTALP-PVA. For IVF, COCs were denuded and groups of 100 oocytes were transferred to 200-μL drops of mTALP-PVA (Long et al. 1999) and incubated with 5,000 motile sperm for 4–6 (Short) or 18–20 h (Long) . Presumptive zygotes were washed and transferred to 100-μL drops of mNCSU-23 (Long et al. 1999) and cultured until Day 4 (Day 0 = IVF) in humidified 5% CO2, 6% O2, 89% N2. Oocyte cleavage was assessed 48 h post-insemination, and on Day 4 selected morulae were transferred to recipient sows (n = 7 Large White × Landrace; 65 morulae per sow) nonsurgically using a Firflex catheter (Magapor, Zaragoza, Spain). Sex of remaining embryos was confirmed by PCR and restriction analysis (Cong et al. 1993 Hum. Mol. Genet. 2 1187–1191). Data from three replicates were arc sin transformed and analyzed by ANOVA. Oocyte cleavage was similar after Short (724/1547; 46.8%) or Long (598/1528; 39.1%) co-incubation. Resort analysis showed sperm to be >91% purity, and all sexed morulae were of the predicted sex (16/16). Delayed return to estrus (>23 days) was observed in 5 recipient sows (71.4%). Fetal sacs were observed by transcutaneous ultrasound in one of these sows. Pre-sexed porcine IVP embryos can be successfully produced using sex-sorted frozen-thawed boar sperm, and these embryos are capable of initiating pregnancies when transferred to recipients. However, further refinement of porcine IVP and ET protocols are required to enable full in vivo development. This work was supported by XY, Inc., Fort Collins, CO, USA.

scholarly journals Effects of Dietary Supplementation of Oregano Essential Oil to Sows on Oxidative Stress Status, Lactation Feed Intake of Sows, and Piglet Performance

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Chengquan Tan ◽  
Hongkui Wei ◽  
Haiqing Sun ◽  
Jiangtao Ao ◽  
Guang Long ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Essential Oil ◽  
Feed Intake ◽  
Average Daily Gain ◽  
Thiobarbituric Acid ◽  
Late Gestation ◽  
Control Group ◽  
Large White ◽  
Oregano Essential Oil ◽  
Oxidative Stress Status

Fifty-four multiparous large white sows were used to determine the effects of supplementing oregano essential oil (OEO) to the gestation and lactation diets on oxidative stress status, lactation feed intake, and their piglet performance. Two groups were fed diets with (OEO;n=28) or without (Control;n=26) supplemental 15 mg/kg OEO during gestation and lactation. The serum levels of reactive oxygen species (ROS) (P<0.05), 8-hydroxy-deoxyguanosine (8-OHdG) (P<0.05), and thiobarbituric acid reactive substances (TBARS) (P<0.05) were higher during gestation (days 90 and 109) and lactation (days 1 and 3) than in early gestation (day 10). Compared with the control group, the OEO diet significantly reduced sows’ serum concentrations of 8-OHdG (P<0.05) and TBARS (P<0.01) on day 1 of lactation. The OEO diet increased the sows’ counts of faecallactobacillus(P<0.001) while reducingEscherichia coli(P<0.001) andEnterococcus(P<0.001). In the third week of lactation the treatment tended to increase sow’s feed intake (P=0.07), which resulted in higher average daily gain (P<0.01) of piglets. Our results demonstrated that there is an increased systemic oxidative stress during late gestation and early lactation of sows. The OEO supplementation to sows’ diet improved performance of their piglets, which may be attributed to the reduced oxidative stress.

scholarly journals Betaine and Isoquinoline Alkaloids Protect against Heat Stress and Colonic Permeability in Growing Pigs

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1024 ◽  
Author(s):  
Hieu Huu Le ◽  
Majid Shakeri ◽  
Hafiz Ansar Rasul Suleria ◽  
Weicheng Zhao ◽  
Rachel Mai McQuade ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heat Stress ◽  
Salivary Cortisol ◽  
Inflammatory Responses ◽  
Growing Pigs ◽  
Interleukin 1Β ◽  
Isoquinoline Alkaloids ◽  
Large White ◽  
Pork Production ◽  
Total Antioxidant

Heat stress (HS) compromises productivity of pork production, in part as a result of increased oxidative stress and inflammatory responses, particularly within the gastrointestinal tract. This study aimed to investigate whether plant-derived betaine and isoquinoline alkaloids could ameliorate HS in pigs. Fifty female Large White × Landrace grower pigs, which were acclimated to control (CON), control plus betaine (BET), or control plus isoquinoline alkaloids (IQA) diets for 14 days were then exposed to heat stress or thermoneutral condition. Both BET and IQA partially ameliorated increases in respiration rate (p = 0.013) and rectal temperature (p = 0.001) associated with HS conditions. Heat stress increased salivary cortisol concentrations and reduced plasma creatinine, lactate, and thyroid hormone concentrations. Heat stress increased colon FD4 permeability, which was reduced by IQA (p = 0.030). Heat stress increased inflammation in the jejunum and ileum, as indicated by elevated interleukin-1β (p = 0.022) in the jejunum and interleukin-1β (p = 0.004) and interleukin-8 (p = 0.001) in the ileum. No differences in plasma total antioxidant capacity (TAC) were observed with HS, but betaine increased plasma TAC compared to IQA. Dietary BET increased betaine concentrations in the jejunum, ileum (p < 0.001 for both), plasma, liver, kidney (p < 0.010 for all), urine (p = 0.002) and tended to be higher in muscle (p = 0.084). Betaine concentration was not influenced by HS, but it tended to be higher in plasma and accumulated in the liver. These data suggest that betaine and isoquinoline alkaloids supplementation ameliorated consequences of heat stress in grower pigs and protected against HS induced increases in colonic permeability.

Seminal plasma oxidative stress biomarkers do not predict boar sperm freezability

Cryobiology ◽  
2018 ◽  
Vol 85 ◽  
pp. 184
Author(s):  
Isabel Barranco ◽  
Cristina Pérez-Patiño ◽  
Junwei Li ◽  
Emilio A. Martínez ◽  
Inmaculada Parrilla ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Seminal Plasma ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Boar Sperm
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