Morphology, physiology and AFLP markers validate that green box is a hybrid of Eucalyptus largiflorens and E. gracilis (Myrtaceae)

2013 ◽  
Vol 26 (2) ◽  
pp. 156 ◽  
Author(s):  
Georgia R. Koerber ◽  
Peter A. Anderson ◽  
Jack V. Seekamp
Keyword(s):  
Nitrogen Isotope ◽  
Aflp Markers ◽  
Putative Hybrid ◽  
Hybrid Index ◽  
Length Polymorphisms ◽  
Murray River ◽  
Species Specific ◽  
Mature Stands ◽  
Physiological Characters ◽  
Green Box

Prolonged drought and salinity on the Chowilla floodplain of the Murray River have caused deterioration of E. largiflorens F.Muell. A putative hybrid with E. gracilis F.Muell, green box, withstands the saline conditions. We aimed to substantiate that green box is a hybrid and to test for agreement between morphological and physiological characters with amplified fragment length polymorphisms (AFLP). Mature stands were measured for leaf, trunk, floral, cotyledon, carbon and nitrogen isotope discrimination, specific leaf area (SLA) and AFLP. Green box was placed between E. largiflorens and E. gracilis according to categorical principal components analysis (CATPCA) of 21 morphological and physiological characters and character states. The hybrid index of 11 AFLP markers that were 78% species specific separated E. gracilis and E. largiflorens, and the majority of green box plants displayed indices ranging from 0.42 to 0.53, reflecting mostly additive inheritance. Calculation of the hybrid index with all 232 AFLP markers, using maximum likelihood, similarly placed green box between E. gracilis and E. largiflorens. Our morphological, physiological and AFLP-marker observations substantiated that green box is a hybrid between E. largiflorens and E. gracilis.

scholarly journals Statistical associations between morphology, physiology and AFLP DNA markers enable selection of a putative eucalypt hybrid able to tolerate salt affected floodplains

2012 ◽  
Vol 61 (1-6) ◽  
pp. 236-246 ◽  
Author(s):  
G. R. Koerber ◽  
T. Hancock
Keyword(s):  
Aflp Markers ◽  
Physiological Traits ◽  
Putative Hybrid ◽  
Naturally Occurring ◽  
Morphological And Physiological Traits ◽  
Component Loadings ◽  
Revegetation Strategies ◽  
Selection Of ◽  
Better Than ◽  
Green Box

Abstract A naturally occurring putative hybrid between Eucalyptus largiflorens F. Muell and Eucalyptus gracilis F. Muell called Green Box tolerates saline conditions of the River Murray floodplains better than E. largiflorens. Revegetation strategies utilizing seedlings of Green Box have had limited success because only a few are Green Box and the majority are throw backs to E. gracilis and E. largiflorens. Therefore, the purpose of this study was to identify traits characteristic of Green Box and AFLP markers associated with the traits enabling selection at the seedling stage. This was done by non-linear canonical correlation analysis (OVERALS) to test for statistically significant associations between morphological and physiological traits with 232 AFLP markers from 9 primer combinations. OVERALS with all markers produced 1st and 2nd dimensions accounting for 80 and 74% of variation respectively. Green Box plants were placed intermediate between E. gracillis and E. largiflorens according to leaf colour, gloss and nitrogen with component loadings (lc) of 0.340, 0.615 and 0.294 respectively. A second approach of simple linear regression of morphological and physiological traits against all 232 AFLP markers singled out 17 with significance P<0.05. Thirteen of these were also identified by OVERALS. Four occurred with high frequency in Green Box and E. largiflorens distinguishing them from E. gracilis. In order to separate Green Box and E. largiflorens, the segregation of a further three markers can be used to align Green Box with E. gracilis. Therefore, the segregation of 7 markers can be utilized to select Green Box.

A putative hybrid of Eucalyptus largiflorens growing on salt- and drought-affected floodplains has reduced specific leaf area and leaf nitrogen

2012 ◽  
Vol 60 (4) ◽  
pp. 358 ◽  
Author(s):  
Georgia R. Koerber ◽  
Jack V. Seekamp ◽  
Peter A. Anderson ◽  
Molly A. Whalen ◽  
Stephen D. Tyerman
Keyword(s):  
Leaf Area ◽  
Specific Leaf Area ◽  
Carbon Isotope Discrimination ◽  
South Australia ◽  
Dry Weight ◽  
Leaf Nitrogen ◽  
Putative Hybrid ◽  
Murray River ◽  
Ambient Co2 ◽  
Green Box

A putative hybrid between Eucalyptus largiflorens F.Muell. and Eucalyptus gracilis F.Muell., called green box, has attracted attention for its ability to grow on the salt- and drought-affected Chowilla floodplain of the Murray River in South Australia. Relationships between carbon isotope discrimination (Δ13C) and the ratio of substomatal to ambient CO2 (ci/ca) indicated that green box was not as water use efficient as E. largiflorens. Specific leaf area of green box and E. gracilis was significantly lower compared with E. largiflorens (38.38 and 36.96 versus 43.71 cm2 g–1). Leaf nitrogen for green box and E. gracilis was significantly lower compared with E. largiflorens (12.66 and 11.35 versus 15.07 mg g–1 dry weight, P = 0.004 and 0.001, respectively) and leaf carbon of E. gracilis was significantly higher compared with green box and E. largiflorens (541.75 versus 514.90 and 519.82 mg g–1 dry weight, P = 0.002 and 0.011 respectively). There were significantly (P = 0.016) more occurrences of elevated ci/ca below a minimum gs in E. gracilis compared with E. largiflorens, with green box being intermediate (means = 21.6, 6.8 and 9.4). After 10 years, E. largiflorens trunk circumference had significantly increased (P = 0.017) and height had significantly decreased (P = 0.026) due to visible dieback. Green box and E. gracilis grew slower, conserving resources, illustrating a useful strategy to consider when choosing plants for revegetation efforts.

Spatial partitioning in the use of structural woody habitat supports the cohabitation of two cod species in a large lowland river

2016 ◽  
Vol 67 (12) ◽  
pp. 1835 ◽  
Author(s):  
J. A. Lieschke ◽  
J. P. Lyon ◽  
P. D. Moloney ◽  
S. J. Nicol
Keyword(s):  
Lowland River ◽  
Specific Interactions ◽  
Negative Consequences ◽  
Catch Per Unit Effort ◽  
Spatial Partitioning ◽  
Murray River ◽  
Eastern Australia ◽  
The Common ◽  
Species Specific ◽  
Murray Cod

Many freshwater fish worldwide have been shown to use Structural Woody Habitat (SWH) for a variety of reasons. The mid reaches of the Murray River, a large lowland river in south-eastern Australia, was surveyed by boat electrofishing, to investigate the use of SWH type (hollows, rootmass and solids), SWH distance to bank (near bank, intermediate to bank and mid-channel) and the interaction between SWH type and distance to bank. The study found that Murray cod catch per unit effort (CPUE) increased in near-bank areas when hollows were a component of the SWH. The CPUE of trout cod was higher when hollows were present. However, the interactions between distance to bank and hollow SWH were complex and dependent on presence or absence of rootmass. The species-specific interactions between SWH microhabitat and distance to bank found within this study has important relevance for stream managers. The common practice of realigning SWH favours Murray cod over trout cod, which could have negative consequences for the endangered trout cod. More broadly, managers may need to consider a balance of SWH type and where it is placed in the river for the species they are targeting when rehabilitating rivers via the introduction of SWH.

AFLP markers and cytotaxonomic analysis reveal hybridisation in the genus Schoenus (Cyperaceae)

Genome ◽  
2002 ◽  
Vol 45 (2) ◽  
pp. 222-228 ◽  
Author(s):  
Ivan Scotti ◽  
Anna Mariani ◽  
Valentino Verona ◽  
Alberto Candolini ◽  
Carlo A Cenci ◽  
...  
Keyword(s):  
Pollen Grains ◽  
Aflp Markers ◽  
Morphological Data ◽  
Hybrid Population ◽  
Putative Hybrid ◽  
Cytological Evidence ◽  
Northeastern Italy ◽  
Natural Range ◽  
The Alps ◽  
Schoenus Nigricans

Molecular, cytological, and morphological data support the existence of a hybrid population between Schoenus nigricans and Schoenus ferrugineus. This population was found in northeastern Italy, where S. nigricans is central with respect to its natural range and S. ferrugineus is marginal, being most common in the Alps and in central and northern Europe. Molecular marker data show that the putative hybrid population is genetically intermediate between nearby populations of the parent species. Cytological evidence confirmed the hybrid nature of this population, as does the almost complete sterility of plants within the population. Although no seeds were produced by the hybrid population, some possibly fertile pollen grains were produced; this suggests that the possibility of introgression between the two species through the hybrids cannot completely be excluded.Key words: Schoenus, AFLP markers, chromosome behaviour, introgression.

scholarly journals Genetic Relationships among Schizolobium parahybum (Vell.) Blake (Leguminosae) Ecotypes from Ecuador and other Countries

2007 ◽  
Vol 56 (1-6) ◽  
pp. 214-221 ◽  
Author(s):  
H. F. Canchignia-Martínez ◽  
S. Hernández-Delgado ◽  
M. González-Paz ◽  
E. Motte ◽  
N. Mayek-Pérez
Keyword(s):  
Costa Rica ◽  
Fragment Length ◽  
Geographical Origin ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Aflp Markers ◽  
Diversity Patterns ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Genetic Status

Abstract Fifteen ecotypes of Schizolobium parahybum (Vell.) Blake collected in Ecuador (9), Brazil (3), Bolivia (1) Costa Rica (1), and Peru (1) were analyzed using Random Amplified Polymorphic DNA (RAPDs), Amplified Fragment Length Polymorphisms (AFLPs) and microsatellites (SSRs) in order to determine their genetic relationships and diversity patterns among ecotypes and to identify the origin of cultivated germplasm in Ecuador. Although AFLP markers were the most informative technique based on amplified products, SSRs clearly differentiated the ecotypes of Ecuador based on their geographical origin or genetic status into two groups: commercial ecotypes growing at western Ecuador very similar to the ecotype from Costa Rica, and native germplasm from eastern Ecuador and ecotypes from Brazil, Peru and Bolivia.

scholarly journals A Minisatellite Sequence within the Propeptide Region of the Vacuolar Carboxypeptidase Y Gene ofSchizosaccharomyces pombe

1998 ◽  
Vol 180 (14) ◽  
pp. 3727-3729 ◽  
Author(s):  
Susham S. Ingavale ◽  
Rupinder Kaur ◽  
Parul Aggarwal ◽  
Anand K. Bachhawat
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Single Locus ◽  
Carboxypeptidase Y ◽  
Experimental Demonstration ◽  
Length Polymorphisms ◽  
Species Specific

ABSTRACT We describe the presence of a minisatellite sequence that displays length polymorphisms in the fission yeast Schizosaccharomyces pombe. The minisatellite sequence was found to reside within the propeptide region of the vacuolar carboxypeptidase Y gene. The minisatellite sequence, which was found only at a single locus, was mitotically stable and displayed length polymorphisms between the two varieties of S. pombe (S. pombe var.pombe and S. pombe var.malidevorans). The minisatellite sequence, however, appeared to be species specific and was absent in other members of theSchizosaccharomyces genus. This report constitutes the first experimental demonstration of the presence of such sequences in yeasts.

scholarly journals Species-specific AFLP markers for identification of Zingiber officinale, Z. montanum and Z. zerumbet (Zingiberaceae)

2011 ◽  
Vol 10 (1) ◽  
pp. 218-229 ◽  
Author(s):  
S. Ghosh ◽  
P.B. Majumder ◽  
S. Sen Mandi
Keyword(s):  
Zingiber Officinale ◽  
Aflp Markers ◽  
Species Specific

Genetic diversity of the Andean blackberry (Rubus glaucus Benth.) in Ecuador assessed by AFLP markers

2020 ◽  
Vol 18 (4) ◽  
pp. 243-250
Author(s):  
Patricia Garrido ◽  
Eduardo Morillo ◽  
Wilson Vásquez-Castillo
Keyword(s):  
Genetic Diversity ◽  
Genetic Resources ◽  
Basic Research ◽  
Aflp Markers ◽  
Issr Primer ◽  
Inter Simple Sequence Repeats ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Main Production ◽  
Production Areas

AbstractAndean blackberry (Rubus glaucus Benth.) is an emerging fruit crop with significant commercial potential. Despite its growing popularity, basic research about its genetic resources and breeding remains insufficient. The aim of this study was to assess the genetic diversity of Andean blackberry cultivars and related berries species from the main production areas in Ecuador. We analysed a total of 106 samples and performed DNA screening with different molecular markers: random-amplified polymorphic DNAs (RAPDs), inter-simple sequence repeats (ISSRs) and a set of representative samples with amplified fragment length polymorphisms (AFLPs). The tested RAPD primers did not reveal any differentiation among accessions identified as R. glaucus, however one ISSR primer was useful to find polymorphisms allowing the selection of 29 accessions for the analysis with AFLP markers. AFLP-M13 technology was used for screen genetic variations among these accessions and eight wild Rubus accessions. We scored 203 bands using five primer combinations; out of these 152 were informative in R. glaucus. AFLP markers clearly distinguish R. glaucus from the screened wild Rubus species, also an unexpected genetic structure was revealed among R. glaucus cultivars. This genetic differentiation and detection of admixed genotypes suggest a possible introgression of wild Rubus species in R. glaucus. Our findings are relevant for blackberry genetic breeding and use of these genetic resources.

Highly polymorphic genetic markers in meadow voles (Microtus pennsylvanicus) revealed by a murine major histocompatibility complex (MHC) probe

1991 ◽  
Vol 69 (1) ◽  
pp. 213-220 ◽  
Author(s):  
Yves Plante ◽  
Peter T. Boag ◽  
Bradley N. White ◽  
Rudy Boonstra
Keyword(s):  
Major Histocompatibility Complex ◽  
Mhc Class Ii ◽  
Population Sample ◽  
Meadow Vole ◽  
Major Histocompatibility ◽  
Genomic Libraries ◽  
Histocompatibility Complex ◽  
Length Polymorphisms ◽  
Species Specific ◽  
High Degree

We performed cross-species hybridization at the major histocompatibility complex (MHC) to evaluate the ability of murine probes to reveal polymorphism in wild meadow vole populations. Genomic hybridization of a mouse MHC class II I-Aα cDNA revealed extensive restriction fragment length polymorphisms when meadow vole genomic DNA was cleaved with BamHI EcoRI, HindIII, or HincII. The polymorphisms were interpreted by analyzing 10 half-sib families, comprising 6 sires, 10 dams, and 34 offspring. At least 26 different alleles were found with HincII in the family material, and the estimated heterozygosity at those sites is 96%. Several other alleles were found in a population sample. The high degree of genetic variation revealed by this system indicates that it is a viable alternative to multilocus "genetic fingerprinting" probes for the analysis of small mammal pedigrees or strains. Several mouse MHC probes are readily available and they provide a quick route to screening populations and remove the need to extract species-specific markers from genomic libraries.

scholarly journals Genetic Diversity in Hypericum and AFLP Markers for Species-Specific Identification of H. perforatum L.

Planta Medica ◽  
2007 ◽  
Vol 73 (15) ◽  
pp. 1614-1621 ◽  
Author(s):  
Ryan Percifield ◽  
Jennifer Hawkins ◽  
Joe-Ann McCoy ◽  
Mark Widrlechner ◽  
Jonathan Wendel
Keyword(s):  
Genetic Diversity ◽  
Aflp Markers ◽  
Specific Identification ◽  
Species Specific
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