Biochemical Markers in the Family Canidae.

1975 ◽  
Vol 23 (3) ◽  
pp. 411 ◽  
Author(s):  
P Clark ◽  
GE Ryan ◽  
AB Czuppon
Keyword(s):  
Genetic Markers ◽  
Biochemical Markers ◽  
Domestic Dogs ◽  
Marked Variation ◽  
Glucosephosphate Isomerase ◽  
Protein Species ◽  
Multiple Forms ◽  
The Family ◽  
Single Protein ◽  
High Degree

One quantitative and nine qualitative genetic markers were investigated in some 200 individuals of the family Canidae, including 34 breeds and species. A high degree of homogeneity was observed and only one marker, transferrin, displayed marked variation. The consistent differences were: wolf (one sample), transferrin; jackal (one sample), transferrin and glucosephosphate isomerase; foxes, albumin, transferrin and glucosephsophate isomerase. Two abnormal transferrins were observed in two siblings among six German short-haired pointers. All other markers were homogeneous, usually single protein species or multiple forms dispersed randomly through the group. The results suggest that domestic dogs and dingoes share a common pool of genes and that all canids but the foxes possess many genes in common. There are indications that the jackal and wolf may differ from other canids in some marker systems.

Glutamate Dehydrogenase of Pisum sativum: Heat-Dependent Interconversion of the Multiple Forms

1984 ◽  
Vol 39 (3-4) ◽  
pp. 257-260 ◽  
Author(s):  
Adelheid Ehmke ◽  
Heinz-Walter Scheid ◽  
Thomas Hartmann
Keyword(s):  
Glutamate Dehydrogenase ◽  
Direct Evidence ◽  
Molecular Forms ◽  
Stable Form ◽  
Protein Species ◽  
Multiple Forms ◽  
Single Protein ◽  
Catalytically Active ◽  
Pea Seeds ◽  
The Individual

Purified NAD-dependent glutamate dehydrogenase (EC 1.4.1.2) from pea seeds shows a pattern of seven catalytically active molecular forms. The individual forms display different heat stabilities. During incubation at 70 to 75 °C in the presence of protective agents (NADH, Ca2+, DTE) the more heat labile forms are converted into the most stable form. This result presents direct evidence that the multiple forms of pea glutamate dehydrogenase represent conform ational variants of a single protein species

The Inscriptions on the Gravestones in the Croatian Villages of Slovakia, Chunovo and Jarovce: The Specificity of Mixing Graphics Systems

2019 ◽  
Vol 14 (1-2) ◽  
pp. 159-173
Author(s):  
Darya Yu. Vashchenko
Keyword(s):  
Foreign Language ◽  
Ethnic Group ◽  
Ethnic Groups ◽  
Native Language ◽  
Close Contact ◽  
Funerary Monuments ◽  
Traditional Spelling ◽  
The Family ◽  
High Degree ◽  
The Village

The article discusses the inscriptions on funerary monuments from the Croatian villages of Cunovo and Jarovce, located in the South of Slovakia, near Bratislava. These inscriptions reflect the complicated sociocultural situation in the region, which is particularly specific due to the fact that this territory was included to Slovakia’s territory only after 1946, while earlier the village was part of Hungary. In addition, the local Croatian ethnic group was actively in close contact with the German and Hungarian communities. At the same time, the orthographic norms of the literary Croatian, German, Hungarian, and Slovak languages, which could potentially be owned by the authors of the inscriptions, differ in many ways, despite the Latin alphabet used on all the gravestones. All this is reflected in the tombstones, representing a high degree of mixing codes. The article identifies the main types of fusion on the monuments: separate orthograms, writing the maiden name of the deceased in the spelling of her native language, the traditional spelling of the family name. In addition, the mixing of codes can be associated with writing feminitives, also order of name and surname within the anthroponym. Moreover, the settlements themselves represent different ethnic groups coexistence within the village. Gravestones from the respective cemeteries also differ from each other in the nature of the prevailing trend of the mixing codes. In Jarovce, where the ethnic groups live compactly, fusion is often presented as a separate foreign language orthograms. In Cunovo, where the ethnic groups constitute a global conglomerate, more traditional presents for a specific family spelling of the names on the monument.

scholarly journals Involvement of loop 5 lysine residues and the N-terminal β-hairpin of the ribotoxin hirsutellin A on its insecticidal activity

2016 ◽  
Vol 397 (2) ◽  
pp. 135-145 ◽  
Author(s):  
Miriam Olombrada ◽  
Lucía García-Ortega ◽  
Javier Lacadena ◽  
Mercedes Oñaderra ◽  
José G. Gavilanes ◽  
...  
Keyword(s):  
Active Sites ◽  
Structural Changes ◽  
The Other ◽  
Spatial Proximity ◽  
Ribonucleolytic Activity ◽  
The Family ◽  
Lysine Residues ◽  
Hirsutella Thompsonii ◽  
Close Spatial Proximity ◽  
High Degree

Abstract Ribotoxins are cytotoxic members of the family of fungal extracellular ribonucleases best represented by RNase T1. They share a high degree of sequence identity and a common structural fold, including the geometric arrangement of their active sites. However, ribotoxins are larger, with a well-defined N-terminal β-hairpin, and display longer and positively charged unstructured loops. These structural differences account for their cytotoxic properties. Unexpectedly, the discovery of hirsutellin A (HtA), a ribotoxin produced by the invertebrate pathogen Hirsutella thompsonii, showed how it was possible to accommodate these features into a shorter amino acid sequence. Examination of HtA N-terminal β-hairpin reveals differences in terms of length, charge, and spatial distribution. Consequently, four different HtA mutants were prepared and characterized. One of them was the result of deleting this hairpin [Δ(8-15)] while the other three affected single Lys residues in its close spatial proximity (K115E, K118E, and K123E). The results obtained support the general conclusion that HtA active site would show a high degree of plasticity, being able to accommodate electrostatic and structural changes not suitable for the other previously known larger ribotoxins, as the variants described here only presented small differences in terms of ribonucleolytic activity and cytotoxicity against cultured insect cells.

INFLUENCE OF BACTRIO-CARRY IN THE FAMILY ON THE COURSE OF ATOPIC DERMATITIS IN A CHILD

2021 ◽  
pp. 114-119
Author(s):  
Vasileva E.A. ◽  
Kuyarov A.A. ◽  
Kuyarov A.V.
Keyword(s):  
Atopic Dermatitis ◽  
Secondary Infection ◽  
Family Members ◽  
Upper Respiratory Tract ◽  
Epidermal Barrier ◽  
The Family ◽  
Mucous Membranes ◽  
High Degree ◽  
Nose And Throat

The pathogenesis of atopic dermatitis (AD) is complex, and at present a unified concept of the development of the disease has not been formed. At the heart of pathogenesis, there is a complex interaction of numerous factors, such as genetic predisposition, dysfunction of the autonomic nervous system and the epidermal barrier. Previously, it has been proven that a high microbial contamination of the affected areas helps to maintain inflammation and aggravates skin dysfunction as a result of chronic inflammation, and favorable factors appear for the attachment of a secondary infection. However, little attention has been paid to dysfunctions of the epidermal barrier, the role of violations of the quantitative and qualitative composition of the microbiota in the development of AD, and this issue remains relevant at the present time. The aim of this study was to assess the effect of the carrier of bacteria on the mucous membranes of the upper respiratory tract in family members on the course of atopic dermatitis in a child. A bacteriological examination of the mucous membranes of the nose and throat was carried out in 34 children with AD and members of their families (74 people). When examining the mucous membranes of the nose and throat in family members of children with AD, it was found that the microflora isolated from family members in more than half of the cases has an increased potential for pathogenicity with a high degree of contamination (CFU lg 3), which forms an increased bacteriological load on the child. The tendency of bacterial carriage was revealed, contributing to the formation in the family of a permanent persistence of microflora with a high potential for pathogenicity. Asymptomatic carriage of bacteria forms an additional epidemiological load on the child, affects the severity of AD and is a risk factor in the development of secondary infection of the skin.

The rise of the land market in medieval south-west Finland

2018 ◽  
Vol 33 (2) ◽  
pp. 153-171
Author(s):  
Mika Kallioinen
Keyword(s):  
Vertical Integration ◽  
Social Groups ◽  
Land Market ◽  
Barriers To Entry ◽  
South West ◽  
The Family ◽  
High Degree

AbstractThis article aims to explain how the market for land functioned in medieval south-west Finland. The data show that in medieval times land was increasingly treated as something to be transferred in return for ready money, albeit within the limits set by the interests of the family. The land market was open to large segments of society, suggesting that barriers to entry were low. It was characterised by strong vertical integration, although asymmetric, as the majority of the transactions took place between participants from different social groups. The article will also consider the high degree of geographical integration in the land market.

scholarly journals An instance of intergeneric copulation in the family Rhopalidae (Heteroptera): structure, functioning and congruence of the genitalia in two different species from the standpoint of the lock-and-key hypothesis

2019 ◽  
Vol 28 (1) ◽  
pp. 3-18
Author(s):  
D.A. Gapon
Keyword(s):  
Seminal Fluid ◽  
Female Genitalia ◽  
Interspecific Crosses ◽  
The Third ◽  
The Family ◽  
Structural Isolation ◽  
High Degree

n event of mating between specimens of Corizus hyoscyami and Rhopalus parumpunctatus, registered in the Novgorod Province of Russia is considered. The structure of the aedeagi in their completely inflated state and the female genitalia, as well as the functioning of the genitalia, are described and illustrated. The process of unfolding and swelling of the aedeagi is traced using the method of hydraulic inflation of the membranous parts with glass microcapillaries. The congruence of the genitalia is discussed for each species, and also for the interspecific crosses in both combinations of sexes and species. Structures of insect terminalia are divided into three groups according to their functions: 1) auxiliary structures; 2) structures providing the mutual fixation of the genitalia; and 3) structures directly performing the function of transferring seminal fluid. It is shown that in the considered variants of interspecific crosses, the parts of the genitalia belonging to the second group have almost the same structure and functioning, and most likely cannot perform the function of structural isolation. The structures of the third group have taxon-specific morphological and functioning traits and a high degree of congruence within each species. Schemes of the functioning of the genitalia in interspecific crosses, speculatively showing the impossibility of normal insemination, are proposed.

scholarly journals Development of a multiplex system for determining 11 genetic markers of predisposition to obesity

2021 ◽  
Vol 57 (3) ◽  
pp. 300-309
Author(s):  
G. V. Sergeev ◽  
I. V. Haidukevich ◽  
A. M. Gorkavaya ◽  
M. D. Ameliyanovich ◽  
I. B. Mosse ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Fto Gene ◽  
Lepr Gene ◽  
High Degree ◽  
Multiplex System

A system has been developed to identify 11 genetic markers associated with the risk of obesity: rs10852521, rs11075990, rs1121980, rs1421085, rs1477196, rs17817449, rs3751812, rs7206790, rs8047395, rs9940128 (FTO gene) and rs1137101 (LEPR gene) by minisequencing (SNaPshot analysis). The conditions for carrying out the amplification and minisequencing reactions, as well as the compositions of the reaction mixtures, were optimized so that the analysis was carried out for all 11 markers simultaneously. The resulting system was tested and showed a high degree of reproducibility and sensitivity required for the detection of these polymorphisms.

scholarly journals Social Roles as Viewed by Speakers of Non-Cognate Languages.

2020 ◽  
Vol 3 (4) ◽  
pp. 71-83
Author(s):  
Olga Leonidovna Byessonova ◽  
◽  
Keyword(s):  
Native Speakers ◽  
Social Roles ◽  
Men And Women ◽  
Professional Activities ◽  
Male Roles ◽  
The Family ◽  
Degree Of Orientation ◽  
Native Speakers Of English ◽  
The Public Sphere ◽  
High Degree

Introduction. The article addresses the reflection of ideas about gender social roles in the conceptual and linguistic worldview of men and women. Materials and methods. The analysis is based on the material of the linguistic experi-ment conducted with native speakers of such English and Ukrainian. Results. The results of the experiment reveal the differences in the perception by the native speakers of English and Ukrainian of gender social roles. As the analysis of the material shows, in the Ukrainian society, the public sphere is associated to a greater de-gree with the professional activities of men and women, and there is a high degree of orientation of women towards motherhood. In English, outside the family, mainly male roles are defined, and the roles of women are mainly family and are in the sphere of personal, emotional ties. Discussion and Conclusions. The analysis of social roles shows that the Ukrainian lin-guistic community, to a greater extent than the English-speaking, is characterized by an orientation towards the traditional patriarchal family, in which a woman is the keeper of the hearth, the organizer of male consumption, and the man is the owner and head of the family. The results obtained in the course of the experiment enable to establish a systemic cor-relation between the language structure and the social structure, to establish correla-tions between the language phenomena and the gender of the communicants.

A single frizzled protein has a dual function in tissue polarity

Development ◽  
1994 ◽  
Vol 120 (7) ◽  
pp. 1883-1893 ◽  
Author(s):  
R.E. Krasnow ◽  
P.N. Adler
Keyword(s):  
Dual Function ◽  
Protein Species ◽  
Hsp70 Promoter ◽  
Tissue Polarity ◽  
Genetic Mosaic ◽  
Single Protein ◽  
Genetic Epistasis ◽  
Pupal Wing ◽  
Separate Protein ◽  
Transgenic Flies

The Drosophila frizzled (fz) gene is required for the development of normal tissue polarity in the epidermis. Genetic epistasis experiments argue that fz is at the top of a regulatory hierarchy that controls the subcellular site for prehair initiation within the cells of the pupal wing (Wong and Adler, 1993; J. Cell Biol. 123, 209–221). Genetic mosaic experiments indicate that fz has both cell autonomous and cell non-autonomous functions that are separately mutable (Vinson and Adler, 1987; Nature 329, 549–551). Two species of fz mRNA have been identified, raising the question as to whether the two functions are provided by a single protein or by two separate protein species. We generated transgenic flies that express each of these mRNAs under the control of an hsp70 promoter. Only one of the transgenes (hsfzI) showed any fz activity. At 29 degrees C, the hsfzI transgene provided almost complete rescue of a null fz mutation, indicating that the protein encoded by this cDNA can fulfill both fz functions. Overexpression of the hsfzI transgene resulted in two distinct tissue polarity phenotypes depending on the time of heat shock.

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