A massively parallel barcoded sequencing pipeline enables generation of the first ORFeome and interactome map for rice
2020 ◽
Vol 117
(21)
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pp. 11836-11842
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Keyword(s):
Systematic mappings of protein interactome networks have provided invaluable functional information for numerous model organisms. Here we developPCR-mediatedLinkage of barcodedAdaptersTo nucleic acidElements forsequencing (PLATE-seq) that serves as a general tool to rapidly sequence thousands of DNA elements. We validate its utility by generating the ORFeome forOryza sativacovering 2,300 genes and constructing a high-quality protein–protein interactome map consisting of 322 interactions between 289 proteins, expanding the known interactions in rice by roughly 50%. Our work paves the way for high-throughput profiling of protein–protein interactions in a wide range of organisms.
2020 ◽
2021 ◽
Keyword(s):
2018 ◽
Vol 18
(20)
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pp. 1719-1736
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2018 ◽
Vol 46
(6)
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pp. 1593-1603
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2013 ◽
pp. 222-238