scholarly journals Plasmid interactions in Staphylococcus aureus: nonadditivity of compatible plasmid DNA pools.

1975 ◽  
Vol 72 (12) ◽  
pp. 5031-5035 ◽  
Author(s):  
C. Ruby ◽  
R. P. Novick
Keyword(s):  
Staphylococcus Aureus ◽  
Plasmid Dna ◽  
Dna Pools ◽  
Compatible Plasmid

scholarly journals Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages

mBio ◽  
2015 ◽  
Vol 6 (3) ◽  
Author(s):  
Ian R. Monk ◽  
Jai J. Tree ◽  
Benjamin P. Howden ◽  
Timothy P. Stinear ◽  
Timothy J. Foster
Keyword(s):  
Staphylococcus Aureus ◽  
Plasmid Dna ◽  
High Efficiency ◽  
Recombinant Dna ◽  
Genetic Manipulation ◽  
Bacterial Pathogen ◽  
Type I ◽  
Content Type ◽  
E Coli ◽  
Adenine Methylation

ABSTRACTStaphylococcus aureusis a prominent global nosocomial and community-acquired bacterial pathogen. A strong restriction barrier presents a major hurdle for the introduction of recombinant DNA into clinical isolates ofS. aureus. Here, we describe the construction and characterization of the IMXXB series ofEscherichia colistrains that mimic the type I adenine methylation profiles ofS. aureusclonal complexes 1, 8, 30, and ST93. The IMXXB strains enable direct, high-efficiency transformation and streamlined genetic manipulation of majorS. aureuslineages.IMPORTANCEThe genetic manipulation of clinicalS. aureusisolates has been hampered due to the presence of restriction modification barriers that detect and subsequently degrade inappropriately methylated DNA. Current methods allow the introduction of plasmid DNA into a limited subset ofS. aureusstrains at high efficiency after passage of plasmid DNA through the restriction-negative, modification-proficient strain RN4220. Here, we have constructed and validated a suite ofE. colistrains that mimic the adenine methylation profiles of different clonal complexes and show high-efficiency plasmid DNA transfer. The ability to bypass RN4220 will reduce the cost and time involved for plasmid transfer intoS. aureus. The IMXXB series ofE. colistrains should expedite the process of mutant construction in diverse genetic backgrounds and allow the application of new techniques to the genetic manipulation ofS. aureus.

Interspecific relationships of antibiotic resistance in Staphylococcus sp.: isolation and comparison of plasmids determining tetracycline resistance in S. aureus and S. epidermidis

1979 ◽  
Vol 25 (12) ◽  
pp. 1468-1475 ◽  
Author(s):  
David J. Groves
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Dna Sequences ◽  
Plasmid Dna ◽  
Tetracycline Resistance ◽  
Cleavage Sites ◽  
Dna Homology ◽  
Reference Plasmid ◽  
High Degree ◽  
Degree Of Similarity

Tetracycline resistance in Staphylococcus aureus and S. epidermidis was confirmed to be determined by plasmids of the same size. Digestion of plasmids from each strain with restriction endonucleases EcoRl, HindIII, and AluI showed a high degree of similarity in their DNA sequences. At least 10 cleavage sites which appear to be common to both plasmids were detected. An additional three cleavage sites appear to be unique to the S. epidermidis plasmid. Further, a survey of recent clinical isolates of tetracycline-resistant staphylococci detected 7 of 10 S. aureus strains and 8 of 9 S. epidermidis strains with plasmids which were of similar size to the purified reference plasmids and which, by hybridization, showed extensive DNA homology to the S. aureus reference plasmid DNA.

scholarly journals Unstable drug resistance inStaphylococcus aureusM4

1981 ◽  
Vol 38 (3) ◽  
pp. 217-223 ◽  
Author(s):  
W. B. Grubb ◽  
D. I. Annear
Keyword(s):  
Staphylococcus Aureus ◽  
Drug Resistance ◽  
Plasmid Dna ◽  
Streptomycin Resistance ◽  
Erythromycin Resistance ◽  
Resistance To Penicillin ◽  
Lincomycin Resistance

SUMMARYStaphylococcus aureusM4 has chromosomal resistance to streptomycin, plasmid-borne resistance to penicillin and tetracycline and probably chromosomal inducible erythromycin resistance. It also has constitutive erythromycin resistance which is unstable and linked to kanamycin and lincomycin resistance and the ability to produce a diffusible pigment. Variants have been isolated which have stable kanamycin, lincomycin and constitutive erythromycin resistance and these do not produce the diffusible pigment. In transduction experiments kanamycin, lincomycin and constitutive erythromycin resistance were always co-transduced together with streptomycin resistance. The transduction frequencies were approximately 100 times higher with the stable variants compared with the parent. The transductants, irrespective of the donor used, all had stable resistance and did not produce the diffusible pigment. Although transduction with UV-irradiated transducing lysates was characteristic of a plasmid, no corresponding plasmid DNA has been detected.

scholarly journals The synthesis and evaluation of the antitumor and antibacterial activity of two novel oxovanadium complexes

2021 ◽  
pp. 174751982110458
Author(s):  
Jing Wang ◽  
Bin Huang ◽  
Liqiang Wang ◽  
Guijjuan Jiang ◽  
Jianxin Cheng ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Biological Activity ◽  
Antitumor Activity ◽  
Plasmid Dna ◽  
Dna Cleavage ◽  
Biofilm Formation ◽  
Cleavage Activity ◽  
Dna Cleavage Activity ◽  
Mcf 7

Two novel oxovanadium(IV) complexes ([VO(hntdtsc)(BPIP)] and [VO(hntdtsc)(MOPIP)] (hntdtsc = 2-hydroxy-1-naphthaldehydethiosemicarbazone, BPIP = 2-(4-bromophenyl)-imidazo[4,5- f]-1,10-phenanthroline, MOPIP = 2-(4-methoxyphenyl)-imidazo[4,5- f]1,10-phenanthroline), are synthesized and characterized. Subsequently, the Methyl Thiazolyl Tetrazolium (MTT) assay is used to investigate the antitumor activity of the ligand and two complexes in vitro.The results indicate that both complexes could significantly inhibit selected tumor cells (SH-SY5Y, MCF-7, and SK-N-SH). In addition, the antibacterial activity of VO(hntdtsc)(BPIP) against Staphylococcus aureus is further investigated. Interestingly, VO(hntdtsc)(BPIP) can efficiently attenuate S. aureus growth and abrogate α-hemolysin secretion and biofilm formation. The plasmid DNA cleavage activity of both complexes is also investigated. The results suggest that supercoiled plasmid DNA is efficiently cleaved after treatment with each complex, which might contribute to the biological activity of these oxovanadium(IV) complexes.

The sensitivity of phage DNA and plasmid DNA for a restriction enzyme from Staphylococcus aureus

1979 ◽  
Vol 45 (1) ◽  
pp. 19-23
Author(s):  
Ellen E. Stobberingh ◽  
J. A. Meijers ◽  
Johanna H. van Kats-Renaud
Keyword(s):  
Staphylococcus Aureus ◽  
Restriction Enzyme ◽  
Plasmid Dna ◽  
Phage Dna
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