Comparison of antimicrobial resistance in thermophilic Campylobacter strains isolated from conventional production and backyard poultry flocks

Campylobacteriosis is the leading cause of human bacterial gastroenteritis, very often associated with poultry consumption. Thermophilic Campylobacter (Campylobacter jejuni and Campylobacter coli) isolates (n = 158) recovered from broiler neck skin and caecal contents in Ireland over a one-year period, resistant to at least one of three clinically relevant antimicrobial classes, were screened for resistance determinants. All ciprofloxacin-resistant isolates (n = 99) harboured the C257T nucleotide mutation (conferring the Thr-86-Ile substitution) in conjunction with other synonymous and nonsynonymous mutations, which may have epidemiological value. The A2075G nucleotide mutation and amino acid substitutions in L4 and L22 were detected in all erythromycin-resistant isolates (n = 5). The tetO gene was detected in 100% (n = 119) of tetracycline-resistant isolates and three of which were found to harbour the mosaic tetracycline resistance gene tetO/32/O. Two streptomycin-resistant C. jejuni isolates (isolated from the same flock) harboured ant(6)-Ib, located in a multidrug resistance genomic island, containing aminoglycoside, streptothricin (satA) and tetracycline resistance genes (truncated tetO and mosaic tetO/32/O). The ant(6)-Ie gene was identified in two streptomycin-resistant C. coli isolates. This study highlights the widespread acquisition of antimicrobial resistance determinants among chicken-associated Campylobacter isolates, through horizontal gene transfer or clonal expansion of resistant lineages. The stability of such resistance determinants is compounded by the fluidity of mobile genetic element.

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Genetic Structure and Antimicrobial Resistance of Escherichia coli and Cryptic Clades in Birds with Diverse Human Associations

Applied and Environmental Microbiology ◽

10.1128/aem.00861-15 ◽

2015 ◽

Vol 81 (15) ◽

pp. 5123-5133 ◽

Cited By ~ 24

Author(s):

Michaela D. J. Blyton ◽

Hongfei Pi ◽

Belinda Vangchhia ◽

Sam Abraham ◽

Darren J. Trott ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Multidrug Resistant ◽

Wild Birds ◽

Phylogenetic Groups ◽

Treatment Protocols ◽

Content Type ◽

Backyard Poultry ◽

Wildlife Rehabilitation ◽

Wilderness Areas

ABSTRACTThe manner and extent to which birds associate with humans may influence the genetic attributes and antimicrobial resistance of their commensalEscherichiacommunities through strain transmission and altered selection pressures. In this study, we determined whether the distribution of the differentEscherichia coliphylogenetic groups and cryptic clades, the occurrence of 49 virulence associated genes, and/or the prevalence of resistance to 12 antimicrobials differed between four groups of birds from Australia with contrasting types of human association. We found that birds sampled in suburban and wilderness areas had similarEscherichiacommunities. TheEscherichiacommunities of backyard domestic poultry were phylogenetically distinct from theEscherichiacommunities sourced from all other birds, with a large proportion (46%) of poultry strains belonging to phylogenetic group A and a significant minority (17%) belonging to the cryptic clades. Wild birds sampled from veterinary and wildlife rehabilitation centers (in-care birds) carriedEscherichiaisolates that possessed particular virulence-associated genes more often thanEscherichiaisolates from birds sampled in suburban and wilderness areas. TheEscherichiaisolates from both the backyard poultry and in-care birds were more likely to be multidrug resistant than theEscherichiaisolates from wild birds. We also detected a multidrug-resistantE. colistrain circulating in a wildlife rehabilitation center, reinforcing the importance of adequate hygiene practices when handling and caring for wildlife. We suggest that the relatively high frequency of antimicrobial resistance in the in-care birds and backyard poultry is due primarily to the use of antimicrobials in these animals, and we recommend that the treatment protocols used for these birds be reviewed.

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Antimicrobial resistance profiling and DNA Amplification Fingerprinting (DAF) of thermophilic Campylobacter spp. in human, poultry and porcine samples from the Cork region of Ireland

Journal of Applied Microbiology ◽

10.1046/j.1365-2672.2000.01153.x ◽

2000 ◽

Vol 89 (5) ◽

pp. 727-734 ◽

Cited By ~ 12

Author(s):

B. Lucey ◽

C. Feurer ◽

P. Greer ◽

P. Moloney ◽

B. Cryan ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Dna Amplification ◽

Thermophilic Campylobacter ◽

Campylobacter Spp

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Antimicrobial resistance and genomic screening of clinical isolates of thermophilic Campylobacter spp. from south-east Queensland, Australia

Journal of Applied Microbiology ◽

10.1046/j.1365-2672.2003.01856.x ◽

2003 ◽

Vol 94 (3) ◽

pp. 495-500 ◽

Cited By ~ 10

Author(s):

D.A. Alfredson ◽

R.J. Akhurst ◽

V. Korolik

Keyword(s):

Antimicrobial Resistance ◽

Clinical Isolates ◽

Thermophilic Campylobacter ◽

Genomic Screening ◽

Campylobacter Spp

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Prevalence of Thermophilic Campylobacter and their Antimicrobial Resistance Profile in Food Animals in Lare District, Nuer Zone, Gambella, Ethiopia

Journal of Drug Research and Development ◽

10.16966/2470-1009.108 ◽

2016 ◽

Vol 1 (2) ◽

Cited By ~ 2

Author(s):

Abamecha A

Keyword(s):

Antimicrobial Resistance ◽

Resistance Profile ◽

Thermophilic Campylobacter ◽

Antimicrobial Resistance Profile ◽

Food Animals

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Antimicrobial resistance in Enterococcus spp. isolated from laying hens of backyard poultry floks

Annals of Agricultural and Environmental Medicine ◽

10.5604/12321966.1185771 ◽

2015 ◽

Vol 22 (4) ◽

pp. 665-669 ◽

Cited By ~ 6

Author(s):

Fabrizio Bertelloni ◽

Claudia Salvadori ◽

Annalisa Moni ◽

Domenico Cerri ◽

Paolo Mani ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Laying Hens ◽

Backyard Poultry ◽

Enterococcus Spp

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Genomic Characterization of Fluoroquinolone-Resistant Thermophilic Campylobacter Strains Isolated from Layer Chicken Feces in Gangneung, South Korea by Whole-Genome Sequencing

Genes ◽

10.3390/genes12081131 ◽

2021 ◽

Vol 12 (8) ◽

pp. 1131

Author(s):

Noel Gahamanyi ◽

Dae-Geun Song ◽

Kye-Yoon Yoon ◽

Leonard E. G. Mboera ◽

Mecky I. Matee ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Whole Genome Sequencing ◽

Genome Sequencing ◽

Resistance Genes ◽

Virulence Genes ◽

Coli Strain ◽

Whole Genome ◽

Thermophilic Campylobacter ◽

Antimicrobial Resistance Genes ◽

Campylobacter Species

Thermophilic Campylobacter species of poultry origin have been associated with up to 80% of human campylobacteriosis cases. Layer chickens have received less attention as possible reservoirs of Campylobacter species. Initially, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of two archived Campylobacter isolates (Campylobacter jejuni strain 200605 and Campylobacter coli strain 200606) from layer chickens to five antimicrobials (ciprofloxacin, nalidixic acid, erythromycin, tetracycline, and gentamicin) were determined using broth microdilution while the presence of selected antimicrobial resistance genes was performed by polymerase chain reaction (PCR) using specific primers. Whole-genome sequencing (WGS) was performed by the Illumina HiSeq X platform. The analysis involved antimicrobial resistance genes, virulome, multilocus sequence typing (MLST), and phylogeny. Both isolates were phenotypically resistant to ciprofloxacin (MIC: 32 vs. 32 µg/mL), nalidixic acid (MIC: 128 vs. 64 µg/mL), and tetracycline (MIC: 64 vs. 64 µg/mL), but sensitive to erythromycin (MIC: 1 vs. 2 µg/mL) and gentamicin (MIC: 0.25 vs. 1 µg/mL) for C. jejuni strain 200605 and C. coli strain 200606, respectively. WGS confirmed C257T mutation in the gyrA gene and the presence of cmeABC complex conferring resistance to FQs in both strains. Both strains also exhibited tet(O) genes associated with tetracycline resistance. Various virulence genes associated with motility, chemotaxis, and capsule formation were found in both isolates. However, the analysis of virulence genes showed that C. jejuni strain 200605 is more virulent than C. coli strain 200606. The MLST showed that C. jejuni strain 200605 belongs to sequence type ST-5229 while C. coli strain 200606 belongs to ST-5935, and both STs are less common. The phylogenetic analysis clustered C. jejuni strain 200605 along with other strains reported in Korea (CP028933 from chicken and CP014344 from human) while C. coli strain 200606 formed a separate cluster with C. coli (CP007181) from turkey. The WGS confirmed FQ-resistance in both strains and showed potential virulence of both strains. Further studies are recommended to understand the reasons behind the regional distribution (Korea, China, and Vietnam) of such rare STs.

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